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Diagnosis of invasive meningococcal disease using the PCR method
Bronská, Eva ; Marešová, Vilma (advisor) ; Rožnovský, Luděk (referee) ; Žemličková, Helena (referee) ; Novák, Ivan (referee)
lntroduction: lnvasive meningococcal disease continues to be a life-threatening condition. Rapid diagnosis is important for the administration of appropriate treatment. Our study focuses on evaluation of importance of PCR method for diagnosis of meningococcal disease. We describe in detail the dynamics of PCR method and its relationship to other diagnostic methods (LA, culture) and to the course of the disease. We also evaluated the influence of previously appl ied antibiotics therapy to results ofthese diagnostic methods. Methods: We have investigated cerebrospinal fluid and serum samples collected during the 1 sl week of hospitalization from patients hospitalized at the department of infectious diseases with laboratory confirmed invasi ve meningococcal disease. Bacterial DNA was isolated from biological material by Qiagen kit and one-step and seminested PCR method was used. PCR products were detected on the 2% gel electrophoresis. In parallel, we performed culture and LA methods. Results: The PCR positivity in cerebrospinal fluid and serum achieved 92 % and 46 %, respectively, LA positivity in cerebrospinal fluid, serum and urine achieved 47 %, 42 % and 24 %, respectively. Culture of CSF and blood was positive only in 35 % and 39 %, respectively. The latest PCR positivity was detected at Day 7 in...
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Organizational culture in terms of preschool education
Žemličková, Helena ; Šikýř, Martin (advisor) ; Vláčil, Jan (referee)
The submitted diploma thesis focuses on the organizational culture in terms of preschool education, particularly at nursery schools. There are specific elements of the organizational culture for preschool education as well as factors that affect organizational culture at nursery schools within the external and internal environment of the school. This thesis diagnoses the organizational culture at a particular nursery school. Based on this diagnosis, we can say that a strong organizational culture can be created even at a nursery school. There is also the relationship between organizational culture, effectiveness of organization and quality of education that implies from the positive evaluation of this nursery school. The thesis draws up recommendations for this specific nursery school, which are focused primarily on communication and foreknowledge. Recommendations are designed for founders of public nursery school as well. In terms of public nursery school, there should not be an argument of a lack of funds from this perspective, there should rather be a better utilization of that a strong organizational culture offers.
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Residential market at a time of financial crisis
Žemličková, Helena ; Čermáková, Klára (advisor) ; Jaklín, Jiří (referee)
The topic of this bachelor thesis is the residential market of the Czech Republic and changes of housing construction at a time of financial crisis. Residential market can be affected not only by financial crisis, but also by other factors that are not influenced by economic recession. The work is firstly aimed at the change of value added tax law in 2008 and its possible impact on the number of completed dwellings. Supply and demand could be influenced not only by these changes but by uncertainty about future form of these changes, too. The second part of this work is aimed at possible impact of demographic structure on started housing construction. There are two time series that are correlated -- the number of inhabitants of the Czech Republic between the ages of 25 to 39 and the number of started dwellings. The results show that newly started housing construction is not depended on the number of inhabitants at determined age group in conditions of the Czech Republic. The conclusion of this thesis on terms of chosen assumptions and used statistical data is that the changing number of started dwellings cannot be caused by development in the demographic structure.
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