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4-stroke CI-engine Con-rod for Truck
Židek, Lukáš ; Svída, David (referee) ; Dundálek, Radim (advisor)
Brief roundup resolution of problem, tendency and new technologies. Elaboration filling proportion and ordering engine and his exploitation. Suggested proportions of con-rod and his constructive resolution with respekt on strength, functional property, production, service and lifetime. Check strength calculation con-rod eye, shank, top, connecting-rod bolt.
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Dynamical Behaviour of Matrix Proteins from Mason-Pfizer Monkey Virus
Srb, Pavel ; Lang, Jan (advisor) ; Žídek, Lukáš (referee) ; Brus, Jiří (referee)
Title: Dynamical behaviour of matrix proteins from Mason-Pfizer Monkey Virus Author: Pavel Srb Department: Department of Low temperature physics Supervisor: doc. RNDr. Jan Lang, PhD. Abstract: We studied the oligomeric properties of betaretroviral nonmyristoylated ma- trix protein (MA) and its R55F mutant from the Mason-Pfizer monkey virus in solution by means of NMR spectroscopy. We have proven that the wild- type (WT) MA forms oligomers in solution. The final model of oligomeriza- tion of the WT MA was derived by concerted use of chemical shift mapping and diffusion-ordered spectroscopy measured on a set of protein samples with varying concentrations. We found that the Mason-Pfizer monkey virus WT MA exists in a monomer-dimer-trimer equilibrium in solution. Further a combination of NMR relaxation measurements and advanced analysis of molecular dynamics simulation trajectory provided an unprecedentedly de- tailed insight into internal mobility of matrix proteins of the Mason-Pfizer monkey virus. Strong evidence have been obtained that the oligomerization capacity of the wild-type matrix protein is closely related to the enhanced dynamics of several parts of its backbone on a nanosecond time scale. In- creased flexibility has been observed for two regions: the loop between he- lices α2 and α3 and the C-terminal...
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Sexual dimorphism in the mouse olfactory system
Kuntová, Barbora ; Stopka, Pavel (advisor) ; Havlíček, Jan (referee) ; Žídek, Lukáš (referee)
Sexually dimorphic behaviour of the house mouse (Mus musculus musculus) relies on various physical and chemical cues, however, chemical signals are the most essential cues for individual recognition and in causing various priming effects on reproductive behaviour of the receiver. House mice belong to macrosmatic mammals, and thus, their sense of smell is highly developed and is able to recognize a wide spectrum of ligands from other individuals and from their surrounding environment. Volatile signals belong to organic compounds that are produced by most tissues, and may have harmfull effects on cells, and thus they are transported out of the body with lipocalin transporters where some of them may function as signals. These volatile signals are able to stimulate chemosensory neuronal receptors, and thus, yield particular responses in neural circuits. The ligand sensing has a differential effect upon males and females, however, it has not been shown yet in wild mice whether these differences are also caused by the variation in receptors and neural processing, or rather by differential expression of signals typical for each sex. The aim of this thesis was to perform comparative analysis of orofacial mucosal tissues to determine the specificity of expression of particular lipocalins. For the first time...
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Structure and function of selected lipocalins and their role in chamical communication in mammals
Stopková, Romana ; Stopka, Pavel (advisor) ; Sedláček, František (referee) ; Žídek, Lukáš (referee)
Sunrulnv or Pu.D.rHnsrs Chemical communicationis facilitated by various substancesand occurs in all organismal forms from cellular to multicellular organisms. This system of communicationspecializedduring evolutionand incorporatedspecificmeansof transferanddetectionof signalsincludingolfactoryreceptorsanddiversificationof specifictransportersof thesemolecules.Amongtransporters,lipocalinsareproteins thatdueto theirspecificbetabanel formingtertiarysffucutrehavehighafinityto bind various volatile ligands for which they have importantrole in chemical communication. MajorUrinaryProteinsarea well knownexamplein miceaswell asOdorantbinding proteins.However,thereare otherlipocalinsin this proteinfamily of which the function is as yet understudied.In my Ph.D. disertation,I aimed at detailed understandingofthe variabilityin lipocalinfamilyandto suggestotherpotentialroles of theseleastknownproteins.I am presentinga reviewpublicationstemmingout of my bio-informaticanalysis,one paperon Major Urinary Proteinsin two speciesof mice and a paperthatis now submittedand which dealswith novel lipocalin (Obp) genes in Myodes glareolus similar to afrodisin - a protein that was previously describedonly in hamsters.This paperis basedon the sequencesthatwereanalyzed and submittedto GenBank.This last resultopenednew streamof...
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Investigation and inhibition of α-synuclein aggregation
Afitska, Kseniia ; Yushchenko, Dmytro (advisor) ; Žídek, Lukáš (referee) ; Bařinka, Cyril (referee)
α-Synuclein (AS) is a small intrinsically disordered protein expressed in neurons and abundantly present in synapses where it is involved in regulation of synaptic vesicle-mediated protein trafficking. Misfolding of AS into amyloid fibrils is a key process in progression of Parkinson's disease (PD), the second most common neurodegenerative disorder which has no cure to date. Inhibition of AS aggregation and blocking of cell-to-cell spreading of AS fibrils is a promising strategy for PD treatment. However, rational design of inhibitors of this type remains complicated due to the lack of thorough knowledge about the mechanisms of aggregation. Therefore, the aim of this thesis was to gain deeper knowledge about AS aggregation and to apply it for developing inhibitors of AS fibrillization. In my work on the mechanisms of AS aggregation, I first determined that the concentration of AS that enables the fibril growth is an order of magnitude lower than the concentration of AS required for initial fibril formation from monomers. I explored fibril disaggregation at AS concentrations below its Kd value, and characterized AS aggregation at low micromolar concentrations. I then investigated how different modifications of AS C-terminus (namely, extensions of various sizes and charges) affect fibril growth and...
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Sexual dimorphism in the mouse olfactory system
Kuntová, Barbora ; Stopka, Pavel (advisor) ; Havlíček, Jan (referee) ; Žídek, Lukáš (referee)
Sexually dimorphic behaviour of the house mouse (Mus musculus musculus) relies on various physical and chemical cues, however, chemical signals are the most essential cues for individual recognition and in causing various priming effects on reproductive behaviour of the receiver. House mice belong to macrosmatic mammals, and thus, their sense of smell is highly developed and is able to recognize a wide spectrum of ligands from other individuals and from their surrounding environment. Volatile signals belong to organic compounds that are produced by most tissues, and may have harmfull effects on cells, and thus they are transported out of the body with lipocalin transporters where some of them may function as signals. These volatile signals are able to stimulate chemosensory neuronal receptors, and thus, yield particular responses in neural circuits. The ligand sensing has a differential effect upon males and females, however, it has not been shown yet in wild mice whether these differences are also caused by the variation in receptors and neural processing, or rather by differential expression of signals typical for each sex. The aim of this thesis was to perform comparative analysis of orofacial mucosal tissues to determine the specificity of expression of particular lipocalins. For the first time...
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Dynamical Behaviour of Matrix Proteins from Mason-Pfizer Monkey Virus
Srb, Pavel ; Lang, Jan (advisor) ; Žídek, Lukáš (referee) ; Brus, Jiří (referee)
Title: Dynamical behaviour of matrix proteins from Mason-Pfizer Monkey Virus Author: Pavel Srb Department: Department of Low temperature physics Supervisor: doc. RNDr. Jan Lang, PhD. Abstract: We studied the oligomeric properties of betaretroviral nonmyristoylated ma- trix protein (MA) and its R55F mutant from the Mason-Pfizer monkey virus in solution by means of NMR spectroscopy. We have proven that the wild- type (WT) MA forms oligomers in solution. The final model of oligomeriza- tion of the WT MA was derived by concerted use of chemical shift mapping and diffusion-ordered spectroscopy measured on a set of protein samples with varying concentrations. We found that the Mason-Pfizer monkey virus WT MA exists in a monomer-dimer-trimer equilibrium in solution. Further a combination of NMR relaxation measurements and advanced analysis of molecular dynamics simulation trajectory provided an unprecedentedly de- tailed insight into internal mobility of matrix proteins of the Mason-Pfizer monkey virus. Strong evidence have been obtained that the oligomerization capacity of the wild-type matrix protein is closely related to the enhanced dynamics of several parts of its backbone on a nanosecond time scale. In- creased flexibility has been observed for two regions: the loop between he- lices α2 and α3 and the C-terminal...
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