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Communication Between Teaching Assisants and Teachers at a Lower-Secondary School
ŠTEFLOVÁ, Jana
The thesis is devoted to communication between teaching assistants and teachers at lowersecondary schools in the Czech Republic. High-quality and honest communication between the assistant and the teacher is one of the key prerequisites for introducing the principles of inclusion into practice. The work is divided into two parts. The theoretical part consists of chapters where basic concepts are defined and the framework of teacher-teaching assistant cooperation is outlined. Next, a qualitative research is presented (five interviews with teaching assistants analysed using IPA).
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Determination of complexation constants of weak electrolyte analytes by Affinity capillary electrophroesis
Ansorge, Martin ; Šteflová, Jana (advisor) ; Ušelová, Kateřina (referee)
Capillary electrophoresis is not only excellent separation technique but it is often advantageously used for determination of physical-chemical parameters of compounds such as complexation constants of analyte and appropriate complexation agent. The most frequently used electrophoretic method for determination of complexation constants is affinity capillary electrophoresis. In the frame of this work the affinity capillary electrophoresis is used for determination of complexation constants of R and S enantiomers of flurbiprofen, particularly both its neutral and fully dissociated form with neutral β- cyclodextrin. For this purpose we utilized classical method introduced in literature, where complexation constants are determined at two pHs, where analyte is fully and partially dissociated, respectively. Simultaneously, the complexation constants were observed by the new method based on multidimensional nonlinear regression, where all data measured at different pHs and β-cyclodextrin concentrations were utilized for evaluation. Results show that classical method can be highly confusing, especially if unsuitable pH is chosen for determination of stability constants of neutral form of analyte. Multidimensional regression method provides substantially more reliable data but with higher error of estimate....
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Guide service quality assessment from the perspective of visually impaired users
ŠTEFLOVÁ, Jana
The bachelor thesis deals with the evaluation of guide service quality from the perspective of users with visual impairment. According to Act No. 108/2006 Coll., on Social Services, as amended, a social service is an activity or a complex of activities that provide assistance and support to people with the aim of integrating them into society or preventing their social exclusion. Matoušek (2003) states that these are short-term or long-term services that can only be used by people with a legitimate claim. The purpose of these services is to improve the quality of life of the user, but also to defend the interests and rights of all citizens of the society. The bachelor thesis is divided into theoretical and practical parts. The theoretical part consists of two chapters. The first chapter contains the terminology and classification of people with visual impairment. There are also possibilities of compensation of visual impairment and at the end of the first chapter the specifics in the mobility of people with visual impairment here are defined. Loss of vision also means mobility restrictions and complications. Restrictions and complications are evident in the overall mobility capacity of each visually impaired individual, especially in the area of spatial orientation and independent motion. Therefore, the elements of spatial orientation and independent motion are described here. The practical part of the thesis contains the goal of the research and a research question. The data was collected through a qualitative survey. These methods were used - semi-structured interviews and document analysis. Observation of the participants was not realized due to an unwillingness to cooperate. Five communication partners voluntarily participated in the research. The aim of the thesis is to evaluate the quality of guide services from the perspective of visually impaired users by qualitative research. In accordance with the defined objective, the following research question was formulated: what factors are essential for visually impaired people in order to perceive guide service as quality service? The following factors emerged from the qualitative analysis: professional competence (guide qualification, guide's attention, mutual respect, communication, empathy, fitness), availability of service at home, flexibility, affordability, security and trust. The bachelor thesis can be used as study material due to the narrow focus on ophthalmopaedia. Furthermore, as a basis for community planning.
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Peak shape distortions in capillary electrophoresis
Kalus, Michal ; Dovhunová, Magda (advisor) ; Šteflová, Jana (referee)
This Bachelor thesis dissertates about the influence of electromigration dispersion and Taylor dispersion to the total peak dispersion. Firstly, it deals with a solution to a new mathematical theory for peaks influenced by the EMD that was discovered in our research group and it experimentally proves the rightness of the solution. Specifically, it deals with comigration of two analytes with similar mobilities. Secondly, the goal was to find how the Taylor dispersion influences the separation process. This opens a way to further research on how these two effects collide and how they influence the system and separation process together.
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Determination binding constant of beta-blockers by capillary zone electrophoresis
Kanizsová, Lívia ; Zusková, Iva (advisor) ; Šteflová, Jana (referee)
Capillary zone electrophoresis identifies and determines various analytes based on their mobilities in a solution of the background electrolyte, which is exposed to an effect of the electric field. Besides that, it is a suitable method for determination of dissociation and complexation (stability) constants. In this paper the adrenergic β-blockers were chosen as analytes. β-blockers have at least one chiral center in the structure and separation of their enantiomers in presence of the chiral selectors by capillary electrophoresis is possible. For the enantioseparation, variously modified β-cyclodextrins are most often added to the background electrolyte. If the enantiomers of β-blockers have a different affinity to the chiral selector, they exhibit different effective mobilities. Based on a dependence of the effective mobility of analytes on the concentration of chiral selector, we are able to determine their stability constants. Key words: β-blockers, cyclodextrins, stability constant, capillary zone electrophoresis
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Study of inclusion complexes of pesticides with cucurbiturils
Smitka, Jaroslav ; Tesařová, Eva (advisor) ; Šteflová, Jana (referee)
In this work we report the formation of inclusion complexes of the pesticides 1-naphthylacetic acid, 1-naphthylacetamide, napropamide and carbaryl with cucurbit[n]uril (n=7 and 8) and -cyclodextrin hosts. We also report results of the photochemistry of these compounds when free in aqueous solution and included within these nano-containers. The formation of inclusion complexes was studied by electrospray ionization mass spectrometry (ESI-MS) which gave us information about stoichiometry of the complexes and about their reactivity in the gas phase. The photodecomposition and formation of products was followed by high performance liquid chromatography coupled to both mass spectrometry (HPLC-MS) and UV/VIS detector, and spectrophotometry. The studied CBs and their host-guest complexes were readily detected in the gas phase by ESI-MS, mainly as single and double charged ions containing either H+ , Na+ or K+ . The assignments were made based on the m/z values of the observed signals and further confirmed by fragmentation (tandem mass spectrometry - MSn ). All pesticides formed 1:1 (host:guest) complexes. Carbaryl, napropamide and 1-naphthylacetamide also formed 1:2 (host:guest) complexes. Under some solution chemistry conditions, namely for concentrations of cucurbiturils above 50 µM and at low acid and...
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Determination of complexation constants of weak electrolyte analytes by Affinity capillary electrophroesis
Ansorge, Martin ; Šteflová, Jana (advisor) ; Ušelová, Kateřina (referee)
Capillary electrophoresis is not only excellent separation technique but it is often advantageously used for determination of physical-chemical parameters of compounds such as complexation constants of analyte and appropriate complexation agent. The most frequently used electrophoretic method for determination of complexation constants is affinity capillary electrophoresis. In the frame of this work the affinity capillary electrophoresis is used for determination of complexation constants of R and S enantiomers of flurbiprofen, particularly both its neutral and fully dissociated form with neutral β- cyclodextrin. For this purpose we utilized classical method introduced in literature, where complexation constants are determined at two pHs, where analyte is fully and partially dissociated, respectively. Simultaneously, the complexation constants were observed by the new method based on multidimensional nonlinear regression, where all data measured at different pHs and β-cyclodextrin concentrations were utilized for evaluation. Results show that classical method can be highly confusing, especially if unsuitable pH is chosen for determination of stability constants of neutral form of analyte. Multidimensional regression method provides substantially more reliable data but with higher error of estimate....
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