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Instagram aesthetics and its interaction with tourism
Šimůnková, Petra ; Šisler, Vít (advisor) ; Wančová, Nina (referee)
The diploma thesis analyzes the effects of instagramization on specific tourist destinations and the ways in which those places deal with this phenomena. The analysis includes five places from all over the world and apart from description of places and strategies of dealing with instagramization, the analysis also provides categorization of its impacts. The thesis also places the relationship between Instagram, its aesthetics and tourism into a broader theoretical framework, and thus introduces their mutual and close connection. Key words: tourism, tourism industry, Instagram, aesthetic, instagramization, instagrammability, over-tourim, Instagram influencers.
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Sociocultural problems of mass tourism in European context
Šimůnková, Petra ; Sládek, Jan (advisor) ; Mazák, Jaromír (referee)
The goal of this bachelor thesis is to show, how does the tourism industry affects social and culture aspects of local communities and how it affects life at those places in general. For analysis, I have chosen three destinations - Venice, Barcelona and Iceland. Venice is an exemplary case which shows us how much can sociocultural problems escalate. In a case of Barcelona and Iceland we can find different approaches in dealing with this phenomenon and also ways how do these problems emerge. Despite culture differences of those places, we can find some similar patterns - for example mobilization and activation of local civil society. The second part of the thesis is based on results of the survey, which reveals opinions on mass tourism in Prague city centre. Key words: tourism, mass tourism, touristification, authenticity, travel, sociocultural problems, Venice, Barcelona, Iceland, Prague.
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Effect of water deficit on the energy content of individual plant organs in selected species of leafy vegetables
Šimůnková, Petra ; Hnilička, František (advisor) ; Česká, Jana (referee)
This work presents the impact of water deficit on dry mass production and photosynthetic accumulation of energy on every single plant´s organs of selected plant species. As a chosen experimental material were taken plants of Spinacea oleracea "Matador" sort and Tetragonia tetragonioides.
Spinacea oleracea and Tetragonia tetragonioides plants were grown in containers 11x11cm large in mixture of garden substrate A with silicon sand (2:1) in 2 variations placed in FAPPZ greenhouses. One variation was for control and second was exposed to stress reaction. Control variation had been watered for whole time of experiment with 50ml of water 2 times per week. Stressed one had been watered after puncturing and 6. taking until the end of experiment.
During the plant´s ontogenetic evolution had been monitored dry mass´s weight on singular plant´s organs. With usage of burnt calorimetry method had been monitored capacity of photosynteticaly accumulated energy in dry mass. Quantity of netto energy had been found out by dry burning adiabatic calorimeter LAGEST MS 10A.
From the gained results emerge, that plants of Tetragonia tetragonioides have 2 times higher weight growth apart from Spinacea oleracea. Control plants of Tetragonia tetragonioides have higher weight by 25,5% (123 mg) compared to the plants growing in water deficit. Likewise higher weight of roots had been observed on control plants (41 mg) compared to the stressed plants (40 g). Control spinach plants had higher weight of above-ground biomass by 19,5% compared to the stressed ones (43 mg). Roots of control plants had lower weight compared to stressed variant by 2960 mg.
From the results emerge, that plants of Tetragonia tetragonioides have higher weight of roots dry mass and above-ground biomass compared to the Spinacea oleracea plants.
Burning heat without ashes was on above-ground biomass of control plants Tetragonia tetragonioide on 15,96 kJ.g-1 level and on stressed plants 14,93 kJ.g-1 . In case of Spinacea oleracea plants was amount of control plants energy 14,98 kJ.g-1 and on stressed 14,21 kJ.g-1.
Burnt heat without root ashes of Tetragonia tetragonioides control plants was higher by 1.11 kJ.g-1 then on stressed ones (13,19 kJ.g-1). In case of Spinacea oleracea plants was amount of roots energy on control plants 11,90 kJ.g-1 and on stressed 11,72 kJ.g-1 .
Based on measurement were verified differences between species in reaction on water deficit and observed characteristic. From measuring emerged, that Spinacea oleracea plants are more sensitive on effect of water deficit compared to the Tetragonia tetragonioides
plants, which is more tolerant. Next thing observed is, that there are differences in amount of dry mass and rate of burnt heat in relation on effect of water deficit.
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Cell cycle arrest as a hallmark of insect diapause: Changes in gene transcription during diapause induction in the drosophilid fly, Chymomyza costata
ŠIMŮNKOVÁ, Petra
The changes of relative mRNA levels of seven different genes, coding for key cell cycle regulatory factors (Cyclins D and E, kinases Wee1 and Myt1, Phosphatase Cdc25 (String), Dacapo (p27), and Pcna) were performed using qRT-PCR method. Two reference genes (Rp49 and ?-tubulin) served as a background. Significant transcriptional response to photoperiodic transfer were observed for two genes. While the relative levels of Dacapo mRNA increased during the rapid entry into G2 arrest, the Pcna expression was significantly downregulated during the beginning of G0/G1 arrest. Moderate transcriptional upregulations of the genes coding for two cell cycle inhibitory kinases, Wee1 and Myt1 accompanied the entry into diapause. The other genes were expressed equally in all photoperiodic conditions.
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Transcriptional analysis of cell cycle regulators during the induction phase of diapause in Chymomyza costata.
ŠIMŮNKOVÁ, Petra
The changes of relative mRNA levels of seven different genes, coding for key cell cycle regulatory factors (Cyclins D and E, kinases Wee1 and Myt1, Phosphatase Cdc25 (String), Dacapo (p27), and Pcna) were performed using qRT-PCR method. Two reference genes (Rp49 and ?-tubulin) served as a background. Significant transcriptional response to photoperiodic transfer were observed for two genes. While the relative levels of Dacapo mRNA increased during the rapid entry into G2 arrest, the Pcna expression was significantly downregulated during the beginning of G0/G1 arrest. Moderate transcriptional upregulations of the genes coding for two cell cycle inhibitory kinases, Wee1 and Myt1 accompanied the entry into diapause. The other genes were expressed equally in all photoperiodic conditions.
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Transcriptional analysis of genes coding for ion pumps during cold acclimation in drosophilid flies
ŠIMŮNKOVÁ, Petra
The relative abundance of mRNA transcripts of genes coding for {$\alpha$} subunit of Na+K+-ATPase and Vha55 (B) subunit of H+-ATPase were examined (using the qRT-PCR method) in third instar larvae of two fly species, Drosophila melanogaster and Chymomyza costata, that were reared at various acclimation conditions. No differences in mRNA levels were observed in D. melanogaster Malpighian tubules and central nervous system (CNS), when the flies were reared at 25°C or 15°C. C. costata revealed the up-regulation of both ion pump genes in the CNS at 15°C (in comparison to 25°C), and also after the transfer from 25°C to 15°C. This result indicates that compensation of ion pumps function took place at moderately low temperature (still permissive for continuation of activity, growth and development). In contrast, down-regulation of both ion pump genes was seen in the CNS of C. costata larvae at temperature of 5°C. It indicates that channel arrest strategy was adopted at very low temperatures (inducing dormancy).
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