Original title: Cell cycle arrest as a hallmark of insect diapause: Changes in gene transcription during diapause induction in the drosophilid fly, Chymomyza costata
Authors: ŠIMŮNKOVÁ, Petra
Document type: Rigorous theses
Year: 2014
Language: eng
Abstract: The changes of relative mRNA levels of seven different genes, coding for key cell cycle regulatory factors (Cyclins D and E, kinases Wee1 and Myt1, Phosphatase Cdc25 (String), Dacapo (p27), and Pcna) were performed using qRT-PCR method. Two reference genes (Rp49 and ?-tubulin) served as a background. Significant transcriptional response to photoperiodic transfer were observed for two genes. While the relative levels of Dacapo mRNA increased during the rapid entry into G2 arrest, the Pcna expression was significantly downregulated during the beginning of G0/G1 arrest. Moderate transcriptional upregulations of the genes coding for two cell cycle inhibitory kinases, Wee1 and Myt1 accompanied the entry into diapause. The other genes were expressed equally in all photoperiodic conditions.
Citation: ŠIMŮNKOVÁ, Petra. Cell cycle arrest as a hallmark of insect diapause: Changes in gene transcription during diapause induction in the drosophilid fly, Chymomyza costata. České Budějovice, 2014. rigorózní práce (RNDr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/36574

Permalink: http://www.nusl.cz/ntk/nusl-170503


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Rigorous theses