|
|
Raman tweezers: principle and applications
Bernatová, Silvie ; Samek, Ota ; Pilát, Zdeněk ; Ježek, Jan ; Kaňka, Jan ; Šiler, Martin ; Zemánek, Pavel
Raman tweezers combines the optical trap for non-contact micromanipulation of micro-objects with Raman spectroscopy for chemical analysis of the sample. This combination presents an efficient tool for concurrent spectroscopic analysis of chemical composition and micromanipulation allowing sorting or probing of mechanical properties of the sample. Raman tweezers were successfully used for example in characterization of biomolecules like DNA, for sorting of cells and investigation of the link between their chemical and mechanical properties.
|
|
| |
|
|
Classical and advanced methods of optical micromanipulations and their applications
Zemánek, Pavel ; Brzobohatý, Oto ; Šiler, Martin ; Karásek, Vítězslav ; Samek, Ota ; Jákl, Petr ; Šerý, Mojmír ; Ježek, Jan
Optical micro-manipulation techniques have been using for more than 30 years to transfer the momentum from light to microparticles or nanoparticles and influence their movement in liquid, on the surface, or in the air. These days such techniques become more developed and frequently used in physics, chemistry and biology to manipulate, trap, rotate, or sort various types of objects, including living cells in a contactless and gentle way.
|
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
|
Pokročilé techniky optických mikromanipulací
Zemánek, Pavel ; Čižmár, Tomáš ; Šiler, Martin ; Jákl, Petr ; Šerý, Mojmír ; Karásek, Vítězslav ; Brzobohatý, Oto
Nowadays the classical tool of optical micro-manipulations - optical tweezers - found numerous applications in physics, biology and chemistry. However new techniques were developed that used more sophisticated laser beam tailoring and enable to modify positions and number of manipulated objects dynamically, to sort optically objects according to their properties, to deliver them over millimetre long distances or even to let them self-organize after light illumination. We present several results dealing with the above mentioned advanced techniques developed in our laboratory.
|
guest ::
