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Cultivation of Limnospira maxima and utilization of its fresh biomass in human nutrition
Vásquez, Claudia
This work aims to enhance productivity, efficiency and sustainability of L. maxima cultivation as well as to determine the safe microbiological conditions for the nutritional use of fresh spirulina biomass. Various parameters such as light intensity, light spectral composition, temperature, pH, CO2, and culture media composition can be optimized. A series of experiments were performed to find the most suitable configuration to cultivate L. maxima, defined by cyanobacterium productivity and yield as well as other metabolites production such as phycocyanin (C-PC) and carotenoids.
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Application of hydrodynamic cavitation in brewing
Štěrba, Jiří
The aim of this work is to apply these advantages in the brewery industry. In the field of beer brewing, we would like to investigate several phenomenona, such as the isomerization of bitter hop acids, the reduction of gluten concentration or the cell-walls breakdown.
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Chiral anion exchanger based on α-methylbenzylamine for enantioselective separation of racemic acids
Čížek, Jan
In this work, new enantioselective material based on α-methylbenzylamine as the chiral selector (recently utilized by our group in the preparation of chiral\nmembranes) covalently attached to Merrifield resin beads is proposed and discussed as a potentially cost-effective alternative to typically expensive stationary phase materials. It is shown that charged chiral methylbenzylamine works as an enantioselective anion exchanger potentially suitable for the separation of chiral acids, such as N–protected amino acid, or non-steroidal anti-inflammatory drugs (NSAID), such as ibuprofen. In a glass column chromatography experiment, racemic N–(tert–butoxycarbonyl) tryptophan was partially separated, reaching enantiomeric excess in eluates of around 10%.\n
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Synthesis and cytotoxicity of fluorinated mannosamines
Krčil, Aleš
The aim of the project is to synthesise mono-, di- and trifluoroanalogs of N-acetylmannosamines. The cytotoxicity of the final products towards selected cancer cells will be determined. The key intermediates are the corresponding multiply fluorinated mannosazidethioglycosides prepared from deoxyfluorinated 1,6-anhydro-2-azidoβ-D-hexopyranose precursors by ring-opening reactions with phenyltrimethylsilyl sulfide.
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Utilization of electrochemically induced cleavage of various sulfonimides
Salvadori, Karolína
In this work, we describe the unexpected electrochemical cleavage of sulfonimides, providing a direct pathway to corresponding sulfonamides. For this purpose, 16 substances were synthesized and tested, reflecting the influence of different electron effects of -SO2Ph-R substituents, and the impact of the number of nosyl units present in molecules. In the case of octakis-nosylated calixarenes, the electrochemical cleavage via direct potential-controlled electrolysis was compared with conventional organic synthetic procedures. After electrolysis, all prepared sulfonamides were isolated in excellent yields.
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Detailed Investigation of Human Galectins -1 and -3 by employing selectively deoxyfluorinated N-acetyllactosamines
Kurfiřt, Martin
This work is focused on a detailed investigation of supramolecular recognition events between human galectins -1 and -3 and monodeoxyfluorinated N-acetyllactosamine probes, which were previously prepared in our laboratory. The recognition was studied using a combination of X-ray crystallography, isothermal titration calorimetry and several advanced NMR techniques, such as 19F T2-filter, 1H–1H STD, 1H–15N CSP or 19F EXSY. These techniques provided insight into the thermodynamics and kinetics of recognition and enabled us to uncover of subtle differences between both investigated galectins. Such differences could be potentially exploited in the selective targeting of individual galectins with important implications for the galectins-focused\npharmaceutic industry.
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Organoruthenium glycomimetics as selective galectin-1 inhibitors
Hamala, Vojtěch
The prepared organoruthenium glycomimetics demonstrated sin gle unit nanomolar affinity to Gal-1 and over four orders of magnitude\nhigher selectivity to Gal-1 in comparison to Gal-3. The organoruthe nium galectin inhibitors were found to be nontoxic to both cancer and\nnoncancerous cell lines. Selected complexes showed an in vitro an timigratory effect against invasive cancer cell lines MDA-MB-231 and\nSK-OV-3.
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How can we best eliminate pharmaceuticals from wastewater?
Bourassi, Mahdi
Persistent pharmaceutical occurrence in different lakes and rivers usually originates from live stocks, agriculture, aquaculture, households, and hospitals. The presence of these compounds in the ecosystems leads to fatal consequences. Pharmaceuticals can threaten microorganisms in the environment, either causing its deterioration or allowing pathogens to gain resistance against antibiotics and medical treatments. These occurrences in the environment questions the ability of conventional treatment to remediate pharmaceutical wastewater.
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Ambient organic aerosol origin at rural background site in the Czech Republic
Lhotka, Radek
Atmospheric aerosols (AA) are ubiquitous particles in the atmosphere that influence the Earth’s climatic system, environmental interactions, and human health. Among AA, great interest is dedicated to organic aerosols (OA) since it can represent from 20 to 90% of total submicron mass. Source apportionment of OA at background sites is one of the important tasks of current air quality protection.
Plný tet: PDF
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