|
|
Possibilities of Laboratory Diagnostics of Lupus Anticoagulant Antibodies in the Special Hematology Laboratory of the Institute for Clinical and Experimental Medicine in Prague
Rybníčková, Soňa ; Fátorová, Ilona (advisor) ; Sadílek, Petr (referee)
Rybníčková Soňa THE POSSIBILITY OF ASSESSMENT OF LUPUS ANTICOAGULANT ANTIBODIES IN HEMATOLOGY LABORATORY IKEM IN PRAGUE Bachelor thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Laboratory Technician Background Diagnosis of the Lupus Anticoagulant is very complicated due to a missing completely reliable screening test and whenever the presence of prolonged clotting times on a routine plasma test is detected, the other potential causes besides LA is needed to exclude. The aim of the thesis is focused on a possibilities of testing the Lupus Anticoagulant in our Clinical Haematology Department in the Institute for Clinical and Experimental Medicine inclusive of ambiguity measured results interpretation and a necessity to abide by the conditions of a preanalytic phase. In addition, clinical manifestation and laboratory criteria assessment as well as a possible interferences in Lupus Anticoagulant testing are also discussed. Methods The theoretical part is focused on a basic mechanism of haemostasis, on an effect of antiphospholipid antibodies, the diagnostic kriteria inclusive of a treatment guidelines. The practical part is focused on importance of the preanalytical phase, principally on necessity of a double centrifugation tube method (to obtain platelet pure plasma). More over is...
|
|
|
Comparison of basic coagulation methods - APTT, PT, FBG by mechanical and optical principle
Hricová, Júlia ; Fátorová, Ilona (advisor) ; Sadílek, Petr (referee)
In my thesis, the basic coagulation methods APTT, PT and fibrinogen were compared with the use of an optical and impedance principle. The coagulation analyser Sysmex CA 7000 was used for measuring coagulation tests using an optical method and the coagulation analyser AMAX Destiny Plus measured the formation of clots using an impedance method. In a theoretical part of my thesis, I described the process of formation of clots as well as the detection principles of the two analysers mentioned. I mainly focused on possible interferences which can influence the values of coagulation tests. I described how these interferences could develop, the mechanism of their influence on measured parameters and how their effects could be removed. In a practical part of my thesis, I described a preanalytic part of blood taking, the coagulation analysers and a principle of their measurement, an internal quality control and the reference values for these tests. For the coagulation tests APTT, PT and fibrinogen were used patient's normal, haemolytic, lipemic and icteric plasma. T-test done in Microsoft Excel was used to assess similarities or differences between the optical and measured impedance results. To see the differences between methods, graphs of mean values of each method were created. For the conclusion of my...
|
|
| |
|
| |
|
|
Implementation of Antiplatelet Therapy Monitoring on the Analyzer Multiplate
Trenčanská, Šárka ; Fátorová, Ilona (advisor) ; Sadílek, Petr (referee)
This study describes the implementation of antiplatelet therapy monitoring on the analyzer Multiplate that works on the principle of impedance aggregometry. We used to determine four tests - ASPItest used for monitoring acetyl salicylic acid (ASA) therapy, ADPtest and ADPtestHS used to monitoring thienopyridines treatment and TRAPtest , which is used to monitoring treatment with GPIIb/IIIa inhibitors. The aim of this study was to verify the accuracy of this method for individual tests, to determine its own reference limits and compare them with literature data and on a group of cardiac patients receiving dual antiplatelet therapy (aspirin 100 mg per day and clopidogrel 75 mg per day) to assess any resistance to these drugs. Accuracy of the method we verified the group of 10 blood donors without any treatment. Each sample was repeatedly measured 5 times and from the measured values we calculated coefficients of variation. Our values of coefficients of variation from the values given by the manufacturer (CV 6 - 10 %) did not differ significantly (ASPItest: CV 8.7 %, ADPtest: CV 8.8 %, ADPtestHS: CV 13.3 % and TRAPtest: CV 12.1 %). We also verified on a group of 32 blood donors without any treatment reference ranges AUC for single tests, which were compared with reference limits from the literature....
|
|
|
Modern Trends in Sample Preparation of Biological Material for Analysis of Selected Biologically Active Compounds
Sadílek, Petr ; Solich, Petr (advisor) ; Nobilis, Milan (referee) ; Barták, Petr (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Petr Sadílek, M.Sc. Supervisor: Prof. RNDr. Petr Solich, CSc. Co-supervisor: Doc. RNDr. Dalibor Šatínský, Ph.D. Title of Doctoral Thesis: Modern Trends in Sample Preparation of Biological Material for Analysis of Selected Biologically Active Compounds This doctoral thesis deals with the determination of selected drugs, their metabolites and other biologically active substances in biological materials. This area of instrumental analysis turns out to be very attractive recently. The reason for this is the need to monitor and optimise treatment and the goal is to achieve the most effective therapy with minimum side effects of drugs for every individual; there are also toxicological reasons. The thesis concentrates on the entire analytical phase of laboratory screening, i.e. not just on development and validation of chromatographical methods of analysis, but also (and especially) on preparation of biological samples prior to the actual analysis. This phase is crucial for analytes assessment, since it determines not only correct results of the laboratory examination, but also influences the lifespan of laboratory equipment, especially chromatographic columns. These days mostly traditional...
|
|
|
The importance of the determination of activated partial thromboplastin time and partial thromboplastin sensitivity compared to the lupus anticoagulant, deficiency of coagulation factors and heparin.
Drábková, Dagmar ; Fátorová, Ilona (advisor) ; Sadílek, Petr (referee)
The importance of the determination of activated partial thromboplastin time and partial thromboplastin sensitivity compared to the lupus anticoagulant, deficiency of coagulation factors and heparin Dagmar Drábková Abstract Activated partial thromboplastin time (aPTT) is coagulation test which detect the disturbances of hemostasis and it is the most common test for monitoring unfractioned heparin therapy. In parcial tromboplastin are present activators of contact phase and phospholipids which can significantly affect the sensitivity of reagents to coagulation factors, lupus anticoagulans and heparin. The objective of this study was firstly to compare the sensitivity of parcial tromboplastins with different origins of fosfolipids and various activators to lupus anticoagulans, to decreased and increased levels of coagulation plasmatic factor VIII and sensitivity to standard heparin. Secondly, according to measured data to determine whether any of the selected reagents is suitable for monitoring heparin while being able to detect deficit of coagulation factors and presence of inhibitor. Determination of aPTT was performed with five different reagents which had various phospholipids and activators. The measurements of aPPT were performed on automatic analyzer BCSXP. From the measured data we have found that...
|
|
|
Search for a suitable housekeeping gene for relative quantification in patients with the chronic lymphocytic leukemia
Ryšková, Barbora ; Vrbacký, Filip (advisor) ; Sadílek, Petr (referee)
5 ABSTRACT Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Barbora Ryšková Supervisor: Mgr. Filip Vrbacký Title of diploma thesis: Search for a suitable houskeeping gene for relative guantification in patients with the chronic lymfocytic leukemia Chronic lymphocytic leukemia (CLL) is the most common form of leukemia found in adults and that is why it is studied very intensively. The cause of CLL is still unknown and it is not known how to cure this lyphoproliferative disorder without high risk of complications that may occur with a bone marrow transplantation. Altered gene expression of angiogenic factors in CLL is still insufficiently known too, so we decided to study this topic in our laboratory. Our task was to establish method for quantification of proper housekeeping gene that can be used for normalization of results of quantification of expression of some angiogenic factors by real-time PCR. We decided to use Abl1 gene but that gene is alternatively spliced. Exon 1 containing variant is housekeeping only and we had to take it into account when choosing proper method. I managed to transform Escherichia coli cells and amplify plasmid containing Abl1 sequence. Plasmid was isolated, quantificated by spectrophotometer and used to...
|
|
| |
|
|
Efficient internet marketing with Google toolbox
Sadílek, Petr ; Stříteský, Václav (advisor) ; Podolka, Luděk (referee)
Quick growth of internet users worldwide as well as significant technological improvements has had an extensive impact on a traditional marketing communication. First part of the thesis focuses on the basic framework that would leverage the internet marketing implementation. In its second part, the Google advertising products (AdWords, Maps, YouTube) and tools(for planning, optimization and implementation) are analyzed and their role in the internet marketing process is explained and demonstrated.
|
guest ::
