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Ekologická rozhraní: interakce živého a neživého v povrchových mikroekosystémech půd a antropogenních substrátů
Univerzita Karlova v Praze, Praha ; Mrázek, Jakub ; Vojta, Jaroslav ; Peksa, Ondřej ; Prášil, Karel ; Kubátová, Alena ; Škaloud, Pavel ; Machová, Kateřina ; Neustupa, Jiří ; Soldán, Zdeněk
V roce 2005 byl studován soubor dvanácti lokalit - tři přírodní stanoviště, pět odkališť, dvě výsypky a dvě kulturní plochy. Předběžné výsledky jednotlivých pracovních skupin: algologické, mykologické, bryologicko-lichenologické a geobotanické.
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Ekologická rozhraní: interakce živého a neživého v povrchových mikroekosystémech půd a antropogenních substrátů
Univerzita Karlova v Praze, Praha ; Peksa, Ondřej ; Vojta, Jaroslav ; Machová, Kateřina ; Škaloud, Pavel ; Prášil, Karel ; Kubátová, Alena ; Neustupa, Jiří ; Soldán, Zdeněk
Výsledky řešení projektu jsou uvedeny v kapitolách: 1. Abiotické a ekofyziologické charakteristiky studovaných lokalit, 2. Studium cyanobakterií a řas, 3. Studium hub, 4. Dekompozice listového opadu břízy a modelové celulózy, 5. Studium lišejníků, 6. Studium mechorostů, 7. Inokulace substrátu sinicí rodu Nostoc. V rámci jednotlivých kapitol jsou pak uvedeny i specifické metodiky. Návrh metodiky Monitoring a management antropogenních toxických substrátů rudních a strusko-popílkových odkališť bez souvislého vegetačního pokryvu.
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The effect of low dose of vinclozolin on reproductive tract development in CD1 outbred mice
Elzeinová, Fatima ; Pěknicová, Jana ; Nováková, Vendula ; Buckiová, Daniela ; Kubátová, Alena
The effect of a low dose of vinclozolin within the development of the reproductive tract during gestation (VIN-GD 15-22) and puberty (VIN-PND 23-44) in CD1 mice was tested. We found a decrease in the anogenital distance, prostate weight and pathology of testes in both experimental groups. Sperm counts decreased to 46% (VIN-GD) and to 81% (VIN-PND), and also the acrosomal state (evaluated by antiacrosomal antibody) decreased in both groups to 89% in comparison to the control group (100%). Sperm head abnormalities increased by approximately 18% and 13%, respectively. In this connection, the expression of some genes was changed (arosome-related gene (Acr), apoptosis related genes (p53, p21)). In conclusion, a low dose of vinclozolin affected the reproductive tract, sperm parameters and expression of selected genes in both experimental groups.
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The effect of low dose of vinclozolin on reproductive tract development in CD1 outbred mice
Pěknicová, Jana ; Elzeinová, Fatima ; Nováková, Vendula ; Buckiová, Daniela ; Kubátová, Alena
The effect of a low dose of vinclozolin within the development of the reproductive tract during gestation (VIN-GD 15-22) and puberty (VIN-PND 23-44) in CD1 mice was tested. We found a decrease in the anogenital distance, prostate weight and pathology of testes in both experimental groups. Sperm counts decreased to 46% (VIN-GD) and to 81% (VIN-PND), and also the acrosomal state (evaluated by antiacrosomal antibody) decreased in both groups to 89% in comparison to the control group (100%). Sperm head abnormalities increased by approximately 18% and 13%, respectively. In this connection, the expression of some genes was changed (arosome-related gene (Acr), apoptosis related genes (p53, p21)). In conclusion, a low dose of vinclozolin affected the reproductive tract, sperm parameters and expression of selected genes in both experimental groups.
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Effect of endocrine disruptors on mammalian fertility
Pěknicová, Jana ; Nováková, Vendula ; Buckiová, Daniela ; Boubelík, Michael ; Elzeinová, Fatima ; Kubátová, Alena
Endocrine system is one of the most important regulatory systems in the mammalian body. The endocrine disruptors (ED) can disrupt the function of the endocrine system but their activity is not a result of the physiological regulation. The effect of low dose of selected ED, bisphenol-A (BPA), p-nonylphenol (NP), diethylstilbestrol (DES), genistein (GEN), resveratrol (RES)), hormone (17α-ethynylestradiol (EE2)) and polybrominated compound (tetrabromobisphenol A (TBBPA)) within development of reproductive tract in outbred CD1 mice was tested. BPA and NP had a negative effect on reproductive organs morphology and mouse spermatozoa. The fertility of mice exposed to DES was disrupted. Contrary to that, treatment by GEN and RES had no effect on the fertility of mice but had some effect on their reproductive parameters except for sperm integrity. Synthetic hormone (EE2) and polybrominated compound (TBBPA) also affected on the organ weight, reproductive parameters and sperm quality.
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Dynamic and role of cytosekeletal proteins in mammalian sperm cells
Paleček, J. ; Dvořáková, K. ; Pěknicová, Jana ; Kubátová, Alena ; Moore, H. D. M.
The cytoskeleton of sperm head consists of resistant structural proteins of the nucleus and of perinuclear theca, which are largely rosponsible for the shaping of the nucleus. three mahjor cyteskeletal proteins, actin, tzubnulin and spectrin, are present in the head of mammalian spermatohzoa. Maoreober, the mictotubular cytoskeleton also contains other dynamic proteins, which have a morphological function within the cell and which are responsible for cell signalling processes.
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Dynamic and role of cytosekeletal proteins in mammalian sperm cells
Paleček, J. ; Dvořáková, K. ; Pěknicová, Jana ; Kubátová, Alena ; Dráber, Pavel
The cytoskeleton of sperm head consists of resistant structural proteins of the nucleus and of perinuclear theca, which are largely rosponsible for the shaping of the nucleus. three mahjor cyteskeletal proteins, actin, tzubnulin and spectrin, are present in the head of mammalian spermatohzoa. Maoreober, the mictotubular cytoskeleton also contains other dynamic proteins, which have a morphological function within the cell and which are responsible for cell signalling processes.
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