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Effect of water stress on gas exchange and fluorescence of selected species of tribe Panicoideae
Hrkotová, Kristýna ; Hnilička, František (advisor) ; Václav, Václav (referee)
Summary
The thesis studied the effect of water deficit on gas exchange in young plants sorghum and foxtail. These crops could replace maize cultivation in some areas, because their use is similar. Our country is currently facing shortage of rainfall. In 2015, this phenomenon was very noticeable. Therefore, it is appropriate to see how the plants of sorghum and foxtail respond to water deficit during their ontogenetic development. On the water deficit are very sensitive plants in the early stages of growth, and therefore the influence of water deficit was observed on young plants of sorghum and foxtail.
Plant genotypes 121, 304, Ruzrok Red and Plant foxtail were divided into four variants: KK (fully irrigated throughout the experiment) KS (14 days full of grout, 10 days water deficit, 4 days full dressing), SK (10 days water deficit, 18 days full of dressing), SS (10 days water deficit, four days filled with grout, water deficit 10 days, four days full of topping). The speed of exchange of gases were measured in the greenhouse device LCpro+ and chlorophyll fluorescence apparatus OS1 - FL. The rate of photosynthesis and transpiration and chlorophyll fluorescence were measured in the morning. The difference between the measured values of the rate of photosynthesis and transpiration value was calculated water use efficiency (WUE).
Most sensitive to water deficit responded genotype 121 which had a value of water use efficiency (WUE) 9.95 (10-3) and also had the genotype and the lowest value of chlorophyll fluorescence and 0.765. Resistant to water deficit seems foxtail plants, which had the lowest photosynthesis difference between control and stressed variant in the range of 14.59 mikromol CO2.m-2.s-1 (SS) to 15.38 mikromol CO2.m-2.s-1 (KK). At the same time it also foxtail lowest difference between chlorophyll fluorescence variant KK (0.814) and SS variant (0.781). From the perspective of transpiration as most resistant to water deficit appeared foxtail (0.08 mmol H2O.m-2.s-1 (SS) to 2.13 mmol H2O.m-2.s-1 (KS)). Conversely, the least resistant genotype was 121, which was in the control variant 1.17 mmol H2O.m-2.s-1 and stressed 1.50 mmol H2O.m-2.s-1.
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The effect of water stress on the content of monosaccharides in potato tubers
Podhorecká, Klára ; Orsák, Matyáš (advisor) ; Hnilička, František (referee)
Bachelor thesis is focused on monitoring of influence on selected metabolites, especially monosaccharides. The first part is devoted to short characterization of potatoes, therefore the importance of this crop, morphology, cultivation and also are described demands of potatoes on environment and nutrition.
In the next section is detailed discussed problems of the chemical composition of tubers with a focusing on carbohydrates. Potatoes are globally consumed and are popular source of carbohydrates. Potatoes are rich in vitamins also, especially in vitamin C, B3 and B6. Potatoes contain a high content of minerals, especially potassium and magnesium, too. As another substances are in potatoes colorants, antioxidants, phenolic and related substances, proteins, and small amount of lipids. It is noted that proteins of the potato tuber are ranked among nutritionally valuable proteins of vegetable origin. Among this proteins is ranked Patatin, which is considered to be a major reserve protein and it is placed in the vacuoles. Substances, which has a negative effect on the human body are called Glycoalkaloids the opposite. Glykoalkaloids are synthesized by plants of the family Solanaceae. The most significant substances in potatoes are alfa-solanine and alfa-chaconine. Value of tubers is primarily determined by their chemical composition, which forms food and raw materials. The contents of sugars in the tubers are relatively low (0,5 % of the original mass), and even though their contents are important both in aspect of manufacturing and in sensory aspect. The main reducing monosaccharides are ranked glucose and fructose, typically with a concentration of 0,15 - 1,5 %. The sugar content significantly affects internal and external quality characteristics especially fried products. Several studies confirm that the accumulation of carbohydrates might help resist extreme stress conditions, especially low temperatures, salinity and water deficiency.
In the next chapter is created summary of stress conditions effects on plants with focusing on potatoes. In plants we can talk about stress, if variability of negative environmental factors exceeds a certain limit. The main theme and also the most limiting factor is deficiency of water in other words water stress. Also were mentioned other factors abiotic and biotic factors marginally.
By effects of stress reaction is created a specific level of adaptive skills. Temporarily it can lead to acclimatization, which increasing the resistance to abiotic stressors. Most of plants are trying to establish the tolerance to stress. In this Chapter were also described changes during storage and during heat treatments of potato, which leads to an undesirable darkening of the tubers. Excessive levels of reducing sugars cause unacceptable browning of fried products.
In the last chapter is described selected methods of determining carbohydrates. These methods are based on reducing properties of sugars, on their condensing reactions by color, measurement of optical activity, measurement of refractive index, separation methods and others.
The methodic of work clarifies the determination of reducing monosaccharides (glucose, fructose), sucrose by the high performance liquid chromatography (HPLC) in four selected varieties of potatoes from harvest of 2015.
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Floristic survey of part natural reserve Sluneční stráň (Ústí nad Labem district)
Štrymplová, Linda ; Česká, Jana (advisor) ; Hnilička, František (referee)
The floristic survey of the part of natural reserve Sluneční stráň (Ústí nad Labem district) was carried out in the vegetation period in 2015.
The studied area is located near the village of Brná in Ústí nad Labem district. Typical is primarily its geobiocenosis of the Bohemian Uplands and xerothermic flora and fauna.
The aim of this thesis was floristic inventory of the given part area and re-inventory of taxa of vascular plants (related to the results of the revision of 1976 and 2012). The main focus was mainly protected and endangered species of studied area. The aim was also the assessment and evaluation of the level of applied and planned management in the context of a valid plan of this natural reserve.
The occurrence of a total of 166 plant species, of which 45 species were determined to be in danger of extinction given in the Red List of vascular plants of the Czech Republic (including 7 kinds of legislatively protected) was detected by inventories in the studied area. Even though, in comparison with previous surveys, there was a partial reduction in species diversity (possible reduction factors are discussed), this location still belongs to very valuable natural areas which must be protected.
Based on the inventory of plant species and its comparison with Habitat Catalogue of the Czech Republic, it was found that mainly a found group formation of secondary grasslands and heathlands represented by the subunits of Rock-outcrop vegetation with Festuca pallens (T3.1) - Association Alysso-Festucion pallentis, and Narrow-leaved dry grasslands (T3.3) - Association Festucion valesiacae (T3.3D - stands without a prominent occurrence of orchids) occurs in the studied area.
To keeping recommended and approved current care plan for the period 2014-2023 can maintain phytodiversity of the communities in this important area from a botanical point of view. In the care plan are itemized in detail suitable and appropriate interventions and protectionist measures.
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Influence of water deficit on physiological characteristics of selected species of green leafy vegetables
Kraus, Kamil ; Hnilička, František (advisor) ; Dana, Dana (referee)
The harmful influences on plants are called stress factors. The stress itself does not slow down only vital functions of plants but influences directly the size and quality of final product as well.
Worldwide, we count the so called abiotic stresses as the most significant crop production stressors. These include in recent years extremely high temperatures and unequal rainfall distribution both areal and during vegetation period.
The water deficit (drought) belongs to the most important stress factors in agriculture. It influences crop production all over the world. Also vegetables are responsive to water deficit, especially the species with huge amount of water in their tissue. Therefore, the goal of this thesis is to assess the impact of water stress on gas exchange and photosynthetic activity of mangold "Beta vulgaris", salad rocket "Eruca sativa" and lettuce "Lactuca sativa", which were placed at water deficit.
The plants - mangold, salad rocket and lettuce - were grown in a partly controlled greenhouse conditions under the Department of Botany and Plant Physiology FAPPZ ČZU in Prague. The plants were grown at a temperature of 25°C in daytime and 18°C at night under natural light conditions. They were grown in containers of size of 11x11 cm in a mixture of garden substrate A and silica sand in the ration 2:1. The experimental scheme included two variants - control and stressed. The control group of plants was watered, and the second half of plants was left under stress for 22 days, when the water deficit was induced by gradual drying for one month, the experiment took place from 1.6 2015 to 22.6 2015. The speed of gas exchange by plants was measured by apparatus LCpro+. From the values of photosynthesis and transpiration water use efficiency (WUE) and chlorophyll fluorescence were calculated.
The gained results proved difference at reaction to water stress between observed kinds of leaf vegetables.
The speed of photosynthesis and transpiration of observed plant species was measured, which decreased due to impact of water deficit. The average speed of photosynthesis of stressed plants was the lowest by lettuce (11,16 "mikro"mol CO2.m-2.s-1), contrarily the highest by mangold (13,2"mikro"mol CO2.m-2.s-1). In case of control plants was the lowest speed of photosynthesis noticed by lettuce (12,03"mikro"mol CO2.m-2.s-1) and the highest by mangold (14,00"mikro"mol CO2.m-2.s-1). In comparison with control group, the speed of photosynthesis of lettuce decreased significantly by impact of water deficit (difference make 7,23 %). On the other hand the lowest decrease of photosynthesis from stressed plants 5,93% was noticed for mangold.
In case of speed of transpiration, it is possible to note, that the lowest transpiration of control group shows mangold with the average speed of transpiration 1,82 mmol H2O.m-2.s-1, by contrast the highest transpiration was measured by lettuce 3,20 mmol H2O. m-2.s-1. In the case of stressed plants moved the average speed of transpiration between 1,54 mmol H2O.m-2.s-1 for lettuce and 1,79 (mmol H2O.m-2.s-1) for mangold. Also, between control and stressed plants of the same species were found differences, and salad rocket reacted to stress of drought more sensitively and mangold less sensitively. For these two plant species was noted decrease of transpiration in the amount of 23,80% and 1,76%.
From the measured value of photosynthesis and transpiration was calculated water use efficiency (WUE). The calculated values show that stressed plants have higher value of WUE in comparison to control plants. The lowest water efficiency from control plants produced salad rocket
(6.51 x 10-3), while the highest mangold (10.31 x 10-3). In the case of stressed plants was the lowest value of WUE noted for salad rocket (8.96 x 10-3), while for mangold was the highest (10.71 x 10-3).
Chlorophyll fluorescence of the experimental plants was also measured. This characteristic was not influenced by plant species, because in case of control plants, there were no differences found between observed plant species. The lowest difference in value of fluorescence had plants of lettuce (0,69) while the highest mangold and salad rocket (0,7). In conclusion it is possible to note, that more sensitive to water deficit are plants of lettuce. As more tolerant to water deficit seems mangold and salad rocket.
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Influence of different lengths and vinification yeast strains on the subsequent conduct of malolactic fermentation
Hornáková, Ľubomíra ; Orsák, Matyáš (advisor) ; Hnilička, František (referee)
The topic of this bachelor thesis is to determine individual organic acids, specifically tartaric, malic and lactic acids in grape must after a final fermentation using high performance liquid chromatography. The reason behind examining the levels of organic acids are various microbiological conditions of fermenting musts with the subsequent influence on the sensoric quality of the resulting wines. In the theoretical section we describe the technological processing of grapes during wine making, characterize the anatomical and chemical structure of the used grapevine, grape microbiota and its influence on the process of organic acid transformations. Separate chapters are dedicated to specific species of the yeasts Saccharomyces cerevisiae, Torulaspora delbrueckii and bacteria Oenococcus oeni for their fundamental place in the fermentation process. The influence of this microbiota is evaluated in the practical section, where the records of individual inoculations are noted and the fermented musts are analyzed using the separational method of high performance liquid chromatography. The levels of organic acids and the length of fermentation gives evidence of the influence of the participating microbiota on the length of wineification and the physico-chemical parameters of fermentation. For the general assesment of efficiency of the used microbiota on individual fermentatated musts, a sensoric evaluation of samples is conducted.
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Influence of growth stimulators on physiological characteristics by hops plants (""Humulus lupulus"" L.)
Bazika, Václav ; Hnilička, František (advisor) ; Josef, Josef (referee)
At growing Saaz variety is the target to reach the highest yield of hop´s cones and high content of alpha acids. The average content of this acids in Saaz variety is in the range from 2,5 to 4,5 %. The growers effort is to reach the higher number of this range because the final selling price is given by content of alpha acids. During the active growth of hops, grower can targeted stimulate the hops to positively affect the physiological processes. Then he can reach better yield of valuable alpha acids and that´s why the target of this diploma thesis was to fix the influence of growth stimulators on physiological processes on the yield of hop´s cones and the yield of alpha acids. And based on the results to identify the suitability of using growth stimulators during the intense hops growing. Following scientific hypothesis were designed: determine the plants reaction diversity on growth stimulator. For reaching the targets and confirm or reject the hypothesis was observed in natural conditions nearby the Chrastany region the plants of Saaz (clone 72) in interaction with the usage of growth stimulators. These stimulators were used: Humastar, Fertileader Vital, Lignoaktivátor. There were four variants: controled with no aplication and three other variants with usage of growth stimulators (for each variant were apllied only one stimulator for three times).
The influence of growth stimulators on hops plants (phase BBCH from 37 to 81) was observed on the basis of the rate of gas exchanges, chlorophyl fluorescence and the leaves chlorophyl content, which were measured by infrared gas analyzer LCpro+ (ADC BioScientific Ltd. Hoddesdon, Great Britain), OS 1-FL (OPTI SCIENCES), Chlorofyl CCM 200 ADC BioScientific Ltd. Hoddesdon, Great Britain) After the crop was evaluated the yield of hop´s cones and the content of alpha acids, beta acids and essentials oils.
The results of measurment indicates that the highest average transpiration rate was observed after Humastar application (+18,3 % against control variant). In the control variant was found the lowest average transpiration rate from all variants. The highest photosynthesis rate was found after Fertileader Vital application (+7 % against control variant). Decrease of photosynthesis rate about 17 % against the control variant was observed after Humastar application. The differences was also observed in chlorophyll fluorescence. The highest Fv/Fm value reached the hop´s plants after Fertileader Vital application, concretely 0,792. On the other hand the lowest Fv/Fm value was observed after Lignoaktivátor application (0,780). After the application of all tested growth stimulators were reached increase of a pigment´s content in hop´s leaves against the control variant. The highest content of pigments were observed after Fertileader Vital application (+19,2 % against control variant). Control variant reached the average content of pigments in hop´s leaves 25,5 chlorophyll units. Differences after the growth stimulation was also observed in yield and quality of dry hop´s cones. The highest yield reached the Lignoaktivátor variant (0,73 t/ha). This is an increase of 17,7 % against the control variant (0,62 t/ha). The yield of hop´s cones was decreased of 8,1 % against the control variant after Humastar application. The similiar trends were also observed in hop´s cones quality. The highest content of alpha acids (3,06 %) was reached after Lignoaktivátor application and at the same time Lignoaktivátor variant also achieved the highest content of essentials oils per 100 g of a sample (0,22 ml). Humastar variant had the lowest content of alpha acids (2,18 %) and also the lowest content of essential oils (0,14 ml).
Based on the results it can be stated that the used growth stimulators had the influence on the physiological characteristics of hop´s plants and also affected the different yield and quality of hop´s cones. Proposed hypothesis were confirmed. Based on the one-year results, application of Fertileader Vital or Lignoaktivátor seems to bee more profitable than Humastar application.
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Factors influencing seed development and maturation
Leová, Phuong Yen ; Pazderů, Kateřina (advisor) ; Hnilička, František (referee)
Cereals are the most common group of grown crops in the world. Winter wheat (Triticum aestivum L.) is the most grown cereal in the Czech republic, represented almost in all growing areas and covers more than a quarter of arable land and over half of grain surfaces. This bachelors's thesis ,,Factors affecting seeds development and maturation" is a compilation based on scientific literature concerning environmental influcences on the development, maturation and germination of seeds. The thesis also describes the basic physiological processes such as germination and dormancy and knowledges about seed vitality and effects of phytohormones.
The main point of this thesis was to determine whether the arrangement of grains per spikelet affect their germination and other seed parametres. The thesis practically deals mainly with the seed germination in 30 ml and 20 ml of water. The experiment was created with the variety of winter wheat named Bohemia and new genotype of winter wheat called MRS or multi row spike.
Seeds were gained by manual scaling out of the spikes and categorized into fractions, according to the position of seed in a spikelet (i. e. first, seconad, third, fourth or fifth).
Seed tests were performed on the folded filter paper Hahnemühle in 30 ml and 20 ml of water. Germination took place at constatnt temperature of 20 °C in air - conditionted box. Measured values were germination percent, mean time of germination and germinating energy. Gained results were statistically evaluated.
Seeds were also analyse with the OmegAnalyzer G, where the point was to determine wheter the position of the grain spikelet affects the content of the seed (nitrogen, gluten, strach). Seed parametres were different not only depending on the position of the seed on the grain spikelet, but also on the variety and amount of water. Comparing the different categories of grains among themselves differences were found. Both in optimal and stress moisture conditions were registred the best results in the Bohemia breed. MRS wheat genotype fared significantly worse in both conditions. The highest average seed germination in 30 ml of water showed third grains of Bohemia breed, which also was the only one able to achieve 100% germination. First grains had the lowest average germination (94 %). MRS in 30 ml of water reached lower values than Bohemia breed. The highest average germination reached the first grains (94%), but neither one grain fraction MRS did not reach 100% germination. The worst levels of seed germination reached second and fifth grains (93%). In 20 ml of water had Bohemia breed lower average germination than average germination in 30 ml of water, even so it still achieved relatively high values. The highest average germination achieved first grains with 98% and the second grains showed the worst germination value (95%). The fastest average germinating had third and fourth grains, second grains had the slowest germination. In 20 ml of water fifth grains germinated the fastest, and also reached the highest germination (95%). Grains of the first fraction were the worst and ended with very low germination (88 %).
Fifth grains had the highest germination energy (90 %) fifth day in 20 ml of water. The lowest values, only 1 % were reached by the first and second grains third day. The fourth and fifth grains showed higher values of germination energy under stressful conditions than in optimal. The first and second grains conversly germinated faster in optimal conditions.
In experimental part of this bachelors's thesis was found that the position of the grains, its weight and size togehter with the different amout of water affects germination. Next the influence of the position of the grains on the grains content wasn't shown.
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Influence of cadmium on the content of selected elements in spring barley (Hordeum sativum L.)
Trsková, Nikol ; Orsák, Matyáš (advisor) ; Hnilička, František (referee)
The theoretical part of the thesis consists of research on the spring barley and chemical composition of its grains. Attention is paid to the stress factors which has inpact on plants such as heavy metals and in particular cadmium and its influence on the plants. The last part deals with the importance of selected trace elements such as copper, iron, manganese and zinc.
The experimental part of the work is devoted to the influence of cadmium contamination on content of selected elements which are copper, iron, manganese and zinc in plants of spring barley. Two experiments were performed. In both experiments, plants were hydroponically cultivated in Knop nutrient solution. Plants were divided into two parts in each experiment, the first part was cultivated without contamination and served as a control samples, the second part was contaminated. The third day of cultivation was added CdCl2.2,5 H2O at a concentration of 10-5 mol/l to the nutrient solution of the second part. In the first experiment the plants were harvested on the 10th day of cultivation and divided into roots, hypocotyls and shoots. In the second experiment were plants harvested on the 13th day of cultivation and separated into roots, hypocotyls, stem base, 1st (oldest), 2nd and 3th (youngest) leaf. From each part of the plant were prepared samples, which were mineralized and consequentely was measured using the ICP-OES method the content of the above mentioned metals in the contaminated and control-sample plants and the content of cadmium in the contaminated plants. The content of cadmium in the control-sample plants was measured by the ETA-AAS. The measured datae were analyzed and described in to the taps and graphs. Highest concentration of cadmiu was discovered in roots of the control sample plants, in case of the first experiment the roots contained 91 %, in the second 95,5 % from the entire amount of cadmium contained in the plant. As a result of the contamination changes in contents of the selected elements ocured. In both of the experimentes the amount of Cu in roots increased, in the upper part of the plants (shoots) the amount was lower comparing
non-contaminated plants. The iron content in the first experiment increased in roots and hypocotyl, in the upper part of the plants (shoots) the content decreased in comparison with control variant. In the second experiment, was the iron content lower in all plant parts. The manganese content was lower in the contaminated plants in almost all parts, only in the first experiment was found minimal increase of manganese content in roots. In both experimentes was found minimal change in content of zinc in roots, in the first experimente increased zinc content in hypocotyls and decreased in the upper part of the plants (shoots), while in the second experiment there was little change of content in hypocotyls but zinc increased in the upper part of the plants (shoots).
The deferences in results of both experimentes may be dependent on varions lenght of the cultivation.
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Natural compounds in foods with anti-cancer effects
Soukup, Josef ; Orsák, Matyáš (advisor) ; Hnilička, František (referee)
In this thesis, I focus on natural substances which are present in commonly available foods and their effects can not only considerably prevent, but also help treat cancer. As this is a very extensive topic, included are only substances which have been studied for this purpose for a long time and which have a large potential in this area. These are phenolic compounds, carotenoids, sulfur compounds, substances found in cruciferous vegetables or green tea, beta-glucans and their sources, and other health beneficial foods such as turmeric, ginger or garlic. I also mention several vitamins (C, E, A, D) which directly show anti-cancer effects or are used in combination with other means to increase the efficiency of the treatment. The deficiency of some other essential vitamins and minerals increase the risk of certain types of cancer as well and a large amount of anti-cancer acting compounds can be also found in herbs, exotic mushrooms, marine organisms and microorganisms, but that area exceeds the content of this thesis. Although nowdays there are substances targeted at only one symptom and can have a certain effect, these medicaments are associated with many side effects and are also expensive. The contemporary paradigm for cancer treatment is either combine several single-targeted medicaments or come up with medicaments that modulate multiple targets. Various types of cancer have similar character, but due to a different etiology of each type and different effect of individual anti-cancer substances (even belonging in the same chemical group), the efficiancy of these compounds against various types of cancer, mostly depending on metabolism, distribution in organism and the target use, differs significantly. Therefore, some substances can show a very strong anti-cancer effect at certain cancer types, while almost none at different types. Other factors such as biological availability or stability make utilization of many compounds more difficult. Basic types of anti-cancer effects are: antioxidant effects (the ability to scavenge free radicals), antiproliferative effects (the inhibition of uncontrolled growth), the ability to induce apoptosis (induction of programmed cell death), the ability to inhibit metastasis and anti-angiogenic effects (prevention of the formation of new blood vessels of the tumor).
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Nootropics
Malík, Matěj ; Orsák, Matyáš (advisor) ; Hnilička, František (referee)
This bachelor thesis deals with the group of substances called nootropics, otherwise known as smart drugs. Nootropics are composed of a diverse group of medicinal substances, which, with their effect, enhance the abilities of human thinking, learning and memorising, especially in cases where these functions are in disorder. In essence and a certain sence, nootropics affect the metabolism of neuronal cells of the CNS. These smart drugs intensify the supply of glucose and oxygen into the brain, increase the synthesis of proteins and nucleic acids in neurons, and enhance the metabolism of phospholipids in neurohormonal membranes. Generally, the desired effect, however, arrives after a longer period of use.
In addition, this work describes the indication of these substances, possible treatments, and their use. Nootropics are divided into four groups: classic nootropic substances, substances increasing brain metabolism, cholinergics and plants with nootropic effect. For each of these groups, the best known members are described more in detail. For the last mentioned group, namely the group of plants with nootropic effect, their collection and processing of herbal drugs is described. Furthermore, the thesis focuses on suitable methods of assessment, mainly the so-called high-performance liquid chromatography technique, known under the abbreviation HPLC. Also mentioned are the legislative measures regarding these substances, such as Act No. 378/2007 Coll. on pharmaceuticals and amendments to some related laws, Decree No. 63/2007 Coll. on payments of pharmaceuticals and foods for special medical purposes, Decree No. 385/2007 Coll. on the establishment of the list of medical substances intended for supporting or additional treatment, and Decree No. 225/2008 Coll. which establishes the requirements for food supplements and food enrichment.
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