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Coherence-controlled holographic microscope in cell's life cycle research
Bartoníček, Jan ; Chmelík, Radim (referee) ; Uhlířová, Hana (advisor)
The subject of the bachelor thesis is live-cell imaging in a transmitted-light holographic microscope which was designed at the Institute of Physical Engineering BUT and comparing this imaging method with the phase-contrast microscopy. The first part is dedicated to a basic description of used imaging techniques and a cell biology. A description of an experiment preparation follows. In the part dedicated to a data analysis the method of dynamic phase differences is described and the method of growth monitoring is proposed. Both methods were used for the analysis of experiments which are described in the last part of this work. Experiments were focused on acquiring time-lapse data of a cell’s cycle and particularly the mitosis.
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Device for wavefront measurement of microscope objective lenses
Bartoníček, Jan ; Šerý, Mojmír (referee) ; Slabý, Tomáš (advisor)
The wavefront reconstruction of a light wave transformed by a microscope objective is the main subject of this diploma thesis together with the design and assembly of a~measuring device and the development of a computational algorithm. The purpose is to determine optical aberrations and to compare a quality of objectives with identical parameters. The term wavefront is explained and its description using Zernike polynomials is introduced in the first part of the thesis. The following part summarizes possible methods for wavefront reconstrucion. Two methods were chosen for experimental determination of a wavefront shape – shearing interferometry and solution of the transport of intensity equation. For each method a brief characteristic is provided together with possible applications, mathematical apparatus, image processing, computational procedure, setup description and proposition and results of experiments. The suitability of both methods for optical aberration determination and microscope objective comparison is discussed. Based on the obtained results, both methods were found to be suitable for comparison of microscope objectives. The suitability for optical aberration determination is possible with certain restrictions.
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Posterior malleolar fractures of the tibia in fracture-dislocations of the ankle
Kostlivý, Karel ; Bartoníček, Jan (advisor) ; Pilný, Jaroslav (referee) ; Dráč, Pavel (referee)
The dissertation deals with fractures of the posterior malleolus of the tibia in ankle fractures. There is no consensus on the issue in the diagnosis and treatment of tibial posterior malleolus fractures in ankle fractures worldwide. The experimental part of the study focused on the anatomy of the ankle joint, especially on the construction of the fibular incisure of the distal tibia as one of the key areas for ankle stability. The result was the determination of the ideal distance for the correct assessment of the position of the fibula in the incisure of the tibia. In the clinical part of the study, the pattern of the fractures was evaluated on a basic group of patients with ankle fractures with concurrent tibial posterior malleolus fracture using preoperative X-ray and CT examination. This made it possible to determine the basic morphological types of posterior malleolus fractures. The resulting classification was verified on an extended group of patients. In clinical practice, it was used to choose the surgical approach and method of fixation. The correctness of the resulting classification was verified on an extended group of patients. The clinical application of the anatomical 3D CT classification achieved a good anatomical position by direct reduction and stable osteosynthesis from the chosen...
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The hip fracture register, optimalization of hip hemiarthroplasty position
Skála - Rosenbaum, Jiří ; Bartoníček, Jan (advisor) ; Chomiak, Jiří (referee) ; Janeček, Miloš (referee)
The study examines the monocentric epidemiological data of patients with hip fractures from 1997-2007. Adult patients treated for hip fracture between the years 1997-2007 were included in the study. Retrospective statistical assessment of continually gathered data focused on epidemiology and demographics. The second part of the study was concerned on risk factors on protrusion of hemiarthroplasty into the acetabulum. The goal was to specify exact indication, proper operative technique and selection of the implant. The study involved 3 683 patients (2 678 women, 1 005 men). Patients older than 70 years accounted for 82 % of all cases. There was an increase in the number of hip fractures resulting in a significant increase in pertrochanteric fractures (AO-31A1+2) (p<0.001). The ratio of trochanteric to neck hip fractures increased from 0.99 to 1.53. Continual monitoring of patients with hip fracture offers data which allows comparisons between regions and countries. The importace of a continual increase in the number of hip fractures has its importance in macroeconomical planning in traumatology. Hemiarthroplasty was conversed to total hip arthroplasty in 12 patients in our department. The indication, time period to conversion and hemiarthroplasty position were retrospectively assessed. The direction of...
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Scapular fractures: Clinical anatomy, radiodiagnostics, classification-it's importance for operative treatment
Tuček, Michal ; Bartoníček, Jan (advisor) ; Paša, Libor (referee) ; Skála-Rosenbaum, Jiří (referee)
Fractures of the scapula are not very frequent and account for less than 0.5 per cent of all fractures. In most cases they are caused by high-energy trauma and are associated with other injuries. Until recently, fractures of the scapula were rather neglected and treated mostly conservatively. Lately, hover the situation has changed. The number of patients with a scapula fracture is increasing, the interest in this issue is growing and discussion focuses more and more on operative treatment of these fractures. The dissertation thesis provides a comprehensive overview of diagnostics, classification and treatment of scapula fractures. In the anatomical section we have described weak areas in the construction of the scapula that predispose incidence of fracture lines. We have studied topographic relations of individual structures on the posterior aspect of the scapula from the viewpoint of the Judet approach and its modifications in individual types of fractures of the scapular body and neck. In the radiological section we have compared efficiency of individual imaging methods with the aim to evaluate the possibilities of individual radiographic methods, 3D CT reconstructions in particular, from the viewpoint of an exact determination of the type of the fracture, number of fragments and their...
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Scapular fractures: Clinical anatomy, radiodiagnostics, classification-it's importance for operative treatment
Tuček, Michal ; Bartoníček, Jan (advisor) ; Paša, Libor (referee) ; Skála-Rosenbaum, Jiří (referee)
Fractures of the scapula are not very frequent and account for less than 0.5 per cent of all fractures. In most cases they are caused by high-energy trauma and are associated with other injuries. Until recently, fractures of the scapula were rather neglected and treated mostly conservatively. Lately, hover the situation has changed. The number of patients with a scapula fracture is increasing, the interest in this issue is growing and discussion focuses more and more on operative treatment of these fractures. The dissertation thesis provides a comprehensive overview of diagnostics, classification and treatment of scapula fractures. In the anatomical section we have described weak areas in the construction of the scapula that predispose incidence of fracture lines. We have studied topographic relations of individual structures on the posterior aspect of the scapula from the viewpoint of the Judet approach and its modifications in individual types of fractures of the scapular body and neck. In the radiological section we have compared efficiency of individual imaging methods with the aim to evaluate the possibilities of individual radiographic methods, 3D CT reconstructions in particular, from the viewpoint of an exact determination of the type of the fracture, number of fragments and their...
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The hip fracture register, optimalization of hip hemiarthroplasty position
Skála - Rosenbaum, Jiří ; Bartoníček, Jan (advisor) ; Chomiak, Jiří (referee) ; Janeček, Miloš (referee)
The study examines the monocentric epidemiological data of patients with hip fractures from 1997-2007. Adult patients treated for hip fracture between the years 1997-2007 were included in the study. Retrospective statistical assessment of continually gathered data focused on epidemiology and demographics. The second part of the study was concerned on risk factors on protrusion of hemiarthroplasty into the acetabulum. The goal was to specify exact indication, proper operative technique and selection of the implant. The study involved 3 683 patients (2 678 women, 1 005 men). Patients older than 70 years accounted for 82 % of all cases. There was an increase in the number of hip fractures resulting in a significant increase in pertrochanteric fractures (AO-31A1+2) (p<0.001). The ratio of trochanteric to neck hip fractures increased from 0.99 to 1.53. Continual monitoring of patients with hip fracture offers data which allows comparisons between regions and countries. The importace of a continual increase in the number of hip fractures has its importance in macroeconomical planning in traumatology. Hemiarthroplasty was conversed to total hip arthroplasty in 12 patients in our department. The indication, time period to conversion and hemiarthroplasty position were retrospectively assessed. The direction of...
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