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Investigation for infectious markers of blood donors in Blood Establishment Hospital Č.B. a.s.
PÍCHOVÁ, Jana
The theme of the bachelor thesis is ?Infectious Disease Marker Testing of Blood Donors?. According legal rules, donated blood is tested for infectious agents of AIDS, syphilis and HBV, HCV. The risk of transmission of such diseases has been decreased in recent decades nevertheless it is still a threat to transfusion medicine. At the Transfusion Department of the České Budějovice Hospital, Inc., the samples of blood donors are tested in the Blood Donors Analytics Laboratory. At present, all the tests are performed on a single instrument Abbott Architect. Before testing on the Architect analyzer, viral markers were tested on the Abbott AxSYM instrument, while syphilis was tested via TP-PA method using a diagnostic kit by Serodia. The objective of the bachelor thesis is to compare the current testing systems with the previous ones, describe individual testing methods, count the numbers of reactive results and compare results of its confirmation tests, find out the frequency of reactive and positive results in new blood donors, verify results of the second confirmation of control samples. The thesis informs about basic criteria to select blood donors, individual infectious markers which cover the infectious diseases, in which a prolonged presence of the agents in blood occurs, and thus the transmission of the infection is possible. Further, the principles of methods used by the AxSYM analyzer, the Architect analyzer and Serodia TP-PA diagnostic kit are described. The samples showing the reactive results are sent to the National Reference Laboratory to be retested. In the period from 1st January 2011 to 31st December 2011 17,262 blood donors were tested using the AxSYM analyzer to detect HIV, HBsAg and HCV infections. The same number of the tests was performed also by TP-PA method to diagnose syphilis. In the period from 1st February 2012 to 31st January 2013, 18,346 of blood donor samples were tested to detect HIV, HBV, HCV and syphilis. 57 samples from the total number of tests performed on the AxSYM analyzer and TP-PA diagnostic kit were reactive in the year 2011. With the installation of the Architect analyzer the numbers of reactive samples increased more than twice, namely to 121 reactive samples. The largest increase in reactivity is evident in case of syphilis diagnostics. It was the similar with anti-HCV method. In both cases, the methods for antibodies detection are involved, and so it can be taken into account the interference with other non-specific antibodies. In the period of testing by TP-PA method on the AxSYM system, 20 reactive control samples, 10 reactive first time blood donors samples and 27 reactive regular donors samples were detected. After the changeover to Architect system, the numbers of reactive samples were increased unexpectedly in regular donors the most, namely up to 88 samples within the monitored period unlike 8 reactive samples in first time donors or 25 reactive control samples. In most cases, the confirmation test results of control samples are negative regardless the type of analyzer used for screening tests showing reactive results. The largest number of unclear results from the National Reference Laboratory (NRL) was observed with anti-HCV method. The NRL confirmed altogether 5 positive results for the monitored infection diseases, from which 4 were in new donors. This finding confirms the assumption that the first time donors are a risk group while with the regular blood donation the safety of blood transfusion is increasing. Based on all observed parameters the both analyzers can be regard as the safe systems for blood donors testing showing the sufficient specificity. The AxSYM analyzer was replaced by the newer Architect analyzer because of upgrading the machine equipment primarily. The chemiluminiscent test technology is preferred because of its high sensitivity, simplicity and reliability.
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Traditional Measures of Diversity, Their Estimates and Sensitivity to Changes
Horáček, Martin ; Zvárová, Jana
We dealt with the traditional measures of diversity and their sample estimates. We proposed a new estimator of measures of diversity and we studied a way to compare sensitivity to changes of different measures of diversity. The proposed estimator compares favorably to other well established estimators. The sensitivity of measure of diversity to changes could be used to compare behavior of different measures of diversity and to select those that are the most suitable for a given problem.
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Quality measures of classification models and their conversion
Hanusek, Lubomír ; Hebák, Petr (advisor) ; Řezanková, Hana (referee) ; Skalská, Hana (referee)
Predictive power of classification models can be evaluated by various measures. The most popular measures in data mining (DM) are Gini coefficient, Kolmogorov-Smirnov statistic and lift. These measures are each based on a completely different way of calculation. If an analyst is used to one of these measures it can be difficult for him to asses the predictive power of a model evaluated by another measure. The aim of this thesis is to develop a method how to convert one performance measure into another. Even though this thesis focuses mainly on the above-mentioned measures, it deals also with other measures like sensitivity, specificity, total accuracy and area under ROC curve. During development of DM models you may need to work with a sample that is stratified by values of the target variable Y instead of working with the whole population containing millions of observations. If you evaluate a model developed on a stratified data you may need to convert these measures to the whole population. This thesis describes a way, how to carry out this conversion. A software application (CPM) enabling all these conversions makes part of this thesis. With this application you can not only convert one performance measure to another, but you can also convert measures calculated on a stratified sample to the whole population. Besides the above mentioned performance measures (sensitivity, specificity, total accuracy, Gini coefficient, Kolmogorov-Smirnov statistic), CPM will also generate confusion matrix and performance charts (lift chart, gains chart, ROC chart and KS chart). This thesis comprises the user manual to this application as well as the web address where the application can be downloaded. The theory described in this thesis was verified on the real data.
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The CZK yield curve analysis and its application for the ALM analyses
Walos, Michal ; Dvořák, Petr (advisor) ; Tuček, Miroslav (referee)
The diploma thesis deals mostly with interest rate risk issue. It describes the basic methods of interest rate risk measurement with use of analyses executing by Asset Liability Management department in banks. Such analyses as repricing GAP, net interest income analysis, market value of equity and sensitivity analyses to interest rate movements. There is an analysis of Czech crown yield curve as well, in order to deeper insight of its probability behaviour. Results of this analysis are used for advanced techniques in ALM. Especially knowledge of volatilities of particular yield points and theirs relations is used in these methods. There was also a multi equation model for predictions of yield curve development created. One of the variables in the model there is the 2-week repo rate of Czech National Bank included.
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Ethical apects of fatherhood.
ČECHOVÁ, Lenka
The work deals with the theme of faterhood from a historical, sociological, psychological and ethical perspective. The importance of the father in child care is defined as the general terms and in terms of specific developmental stages of the child and his female or male sex. The central part of the work consists of the basic characteristics of the ethical aspects that should be applied when the father is caring the child. Final summary is devoted to issue of gay fathers and the dilemma, which is associated with the possiblity to enable them to educate children.
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Influence of collimator type to scintillation camera parameters
ŠTĚDROVÁ, Veronika
Bachelor work Influence of collimator type to scintillation camera is focused especially on experimental measurement and elaboration of results. In theoretical section I deal with a base principle of scintigraphy, describing of Gamma camera, single types of collimators, splitting of collimators according to a type of construction, and according to spatial resolution and sensitivity. In other sections there are examined exact methodology of measurements sensitivity of collimator and spatial resolution and results of measurements. The results are compared with specification given by producers and values published by other authors. In conclusion I recommend the essential type of collimator for nuclear medicine department.
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