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Evaluation of production costs, revenues and quality of potato tuber in organic and conventional farming system
MALECHA, Pavel
In 2009, there was an experiment with strains of potatos conducted in the region of Soběslav, at an altitude of 614 m. The aim of this thesis was to evaluate production costs, revenues and internal tuber quality in terms of organic and conventional farming system. The work observed dry matter, starch, reducing sugar, chlorogenic acid, vitamin C, nitrates and solanine. An attempt was made on two different strains of potatoes which have different growing season (Impala - very early, Adela - early). Adela variant showed an average of 7.51 t / ha higher yield than the Impala. Higher costs per tonne of potato production were in the conventional way of cultivation. From the evaluation of the internal quality of tubers, there was an apparent influence of the strain and cultivation method on the content of controlled substances. The executed test showed the health of potatoes grown in an organic and conventional way.
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The application of AFLP fingerprinting in breeding of Brassica napus
CUŘÍNOVÁ, Petra
AFLP markers are widely used in breeding in some other crops, but their utilization in breeding of Brassica crops is not so frequent. AFLP markers are used for molecular characterization of particular varieties or genotypes and for evaluation of genetic diversity. The aim of this thesis was the application of this method in breeding of rapeseed and in comparative study of genetic variability of different oil seed rape cultivars of Czech, Czechoslovak and German origin. AFLP is based on selective amplification of genomic DNA. Technique consists of three basic steps: 1. restriction of genomic DNA on smaller fragments and ligation of adaptors; 2. selective amplification of fragments with specific primers and 3. electrophoresis of products and computer analysis (Vos et al., 1995). In this thesis 16 Czech (CZ) and Czechoslovak (CZS) and 16 German (G) cultivars of oilseed rape were compared. Two specific primers, EcoRI AGC and fluorescence labeled primer MseI ACC, were chosen for AFLP method. It was possible to distinguish group of CZ/CZS and G cultivars, which have origin in gene resources from Czech Republic and Germany. AFLP technique allows detection of very small differences in Brassica genome. The differences between studied cultivars exist, but they are not very distinct. They show on certain relationship and reduction of genetic basis of genetic resources, which are used in Central Europe.
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