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Dynamic Model for Production of Polyhydroxyalkanoates by Thermophilic Bacterium S. thermodepolymerans
Křápková, Monika ; Šafránek, David (oponent) ; Sedlář, Karel (vedoucí práce)
This master's thesis deals with the reconstruction of a dynamic model for production of polyhydroxyalkanoates (PHA) by thermophilic bacterium Schlegelella thermodepolymerans. The first chapter provides readers with a brief introduction into the systems biology and mathematical graph theory. It is followed by Chapter Two dealing with different approaches in dynamic modelling, including the commonly used tools for dynamic analysis of complex systems. The third chapter then pursues further terms and possibilities regarding the model analysis. The following chapter focuses on metabolomics and the frequently used laboratory techniques and the fifth chapter is then occupied with polyhydroxyalkanoates, especially their chemical structure and properties. In Chapter Six, a general Boolean model for PHA production by thermophilic bacteria is proposed. Chapter Seven then aims at model refinement with focus on S. thermodepolymerans. The final dynamic model is analysed and the results are discussed.
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Biotechnological conversion of spent brewery grains into microbial polymers
Bystrická, Klaudia ; Nováčková, Ivana (oponent) ; Obruča, Stanislav (vedoucí práce)
The presented bachelor thesis deals with the study of biotechnological production of polyhydroxyalkoanates (PHA), using hydrolysates from brewer’s spelt grain (BSG) and selected bacterial strains Schlegelella, Halomonas, Tepidimonas and Burkholderia. The thesis aims to determine whether it is possible to use BSG hydrolysates as a substrate for PHA production, with the emphasis on different pretreatment methods of the lignocellulosic material. BSG was submitted to quantitative analysis and the optimum temperature and pH of the commercial enzymatic cocktail used for the hydrolysation was determined. The most effective transformation of sugars to PHA was accomplished by bacteria H. organivorans with the use of a hydrolysate from plasma pretreated BSG. Hydrolysates which were prepared with moderate methods, contained less bacterial inhibitors and sufficient amounts of sugars. These hydrolysates served therefore as preferable substrates for the microorganism growth.
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Biotechnological conversion of spent brewery grains into microbial polymers
Bystrická, Klaudia ; Nováčková, Ivana (oponent) ; Obruča, Stanislav (vedoucí práce)
The presented bachelor thesis deals with the study of biotechnological production of polyhydroxyalkoanates (PHA), using hydrolysates from brewer’s spelt grain (BSG) and selected bacterial strains Schlegelella, Halomonas, Tepidimonas and Burkholderia. The thesis aims to determine whether it is possible to use BSG hydrolysates as a substrate for PHA production, with the emphasis on different pretreatment methods of the lignocellulosic material. BSG was submitted to quantitative analysis and the optimum temperature and pH of the commercial enzymatic cocktail used for the hydrolysation was determined. The most effective transformation of sugars to PHA was accomplished by bacteria H. organivorans with the use of a hydrolysate from plasma pretreated BSG. Hydrolysates which were prepared with moderate methods, contained less bacterial inhibitors and sufficient amounts of sugars. These hydrolysates served therefore as preferable substrates for the microorganism growth.
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Dynamic Model for Production of Polyhydroxyalkanoates by Thermophilic Bacterium S. thermodepolymerans
Křápková, Monika ; Šafránek, David (oponent) ; Sedlář, Karel (vedoucí práce)
This master's thesis deals with the reconstruction of a dynamic model for production of polyhydroxyalkanoates (PHA) by thermophilic bacterium Schlegelella thermodepolymerans. The first chapter provides readers with a brief introduction into the systems biology and mathematical graph theory. It is followed by Chapter Two dealing with different approaches in dynamic modelling, including the commonly used tools for dynamic analysis of complex systems. The third chapter then pursues further terms and possibilities regarding the model analysis. The following chapter focuses on metabolomics and the frequently used laboratory techniques and the fifth chapter is then occupied with polyhydroxyalkanoates, especially their chemical structure and properties. In Chapter Six, a general Boolean model for PHA production by thermophilic bacteria is proposed. Chapter Seven then aims at model refinement with focus on S. thermodepolymerans. The final dynamic model is analysed and the results are discussed.
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