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In vitro and in vivo studies of the neuroprotective peptide humanin using 125I-radiolabeled humanin derivatives
Evangelou, A. ; Zikos, C. ; Benaki, D. ; Pelecanou, M. ; Bouziotis, P. ; Papadopoulos, M. ; Borovičková, Lenka ; Veselá, Iva ; Elbert, Tomáš ; Kunešová, G. ; Pirmettis, I. ; Paravatou-Petsotas, M. ; Slaninová, Jiřina ; Livaniou, E.
Four new HN-derivatives were synthesized: Y-PAGASRLLLTGEIDLP (Y-des-Leu-RG-PAGA, 1), Y-PAGASRLLLLTGEIDLP (Y-RG-PAGA, 2), Y-SALLRSIPAPAGASRLLLTGEIDLP (Y-des-Leu-Colivelin, 3), Y-SALLRSIPAPAGASRLLLLTGEIDLP (Y-Colivelin, 4), their neuroprotective action was evaluated in the T-maze test and the most active ones were monoradioiodinated with 125I and the labelled peptides used for binding and biodistribution studies.
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Specific binding of prolactin-releasing peptide analogues in pituitary cells
Maixnerová, Jana ; Špolcová, Andrea ; Matyšková, Resha ; Blechová, Miroslava ; Veselá, Iva ; Železná, Blanka ; Maletínská, Lenka
We report specific binding of 125I-PrRP31 to the following cell lines: GH3 cells (rat – somatotrophs), AtT20 cells (mouse – adenocorticotrophs) and RC-4B/C cells (rat- somatotrophs, lactrophs, adenocortitrophs and gonadotrophs). These results will serve as a basic knowledge for future structure-activity studie sof PrRP.
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Labeling of peptides by Na125I-IODO-GENtm system
Veselá, Iva ; Elbert, Tomáš
Very high excess of IODO-GEN(tm) reagent (115 nmol in commercially available precoated tubes from Pierce) leads to damage of oxidation sensitive peptides containing tryptophan residues. Using 5 to 23 nmol of IODO-GEN(tm) in a reaction with 1 to 4 mCi of Na[125-I] and 10 to 30 nmol of peptide leads to reproducible results and reproducible yields.
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