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Hmotnostní spektrometrie v lipidomice
Cvačka, Josef
Podrobně jsou diskutovány současné postupy analytické chemie v lipidomice založené na hmotnostní spektrometrii a chromatografii. Jsou uvedeny příklady kvalitativních a kvantitativních metod včetně hmotnostně-spektrometrického zobrazování.
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Analysis of plant membrane lipids by RP-HPLC/HR-ESI-MS
Zábranská, Marie ; Vrkoslav, Vladimír ; Cvačka, Josef
Monogalactosyldiacylglycerols (MGDGs) and digalactosyldiacylglycerols (DGDGs) are glycolipids forming the major part of biological membranes in photosynthesizing organisms. The individual species differ from fatty acids linked to sn-1 and sn-2 possition on glycerol backbone. Complexity of their mixtures is increased by enzymatic or chemical oxidation of unsaturated species. The aim of this work was to develop and optimize RP-HPLC/HR-ESI-MS2 methods for analysis of oxidized and non-oxidized MGDGs and DGDGs in plant samples. MGDGs and DGDGs were isolated from Arabidopsis thaliana and Melissa officinalis.
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New peptides with antimicrobial activity isolated from the venom of the bumblebee Bombus pascuorum (Hymenoptera: Apidae)
Ježek, Rudolf ; Janechová, D. ; Cvačka, Josef ; Slaninová, Jiřina ; Voburka, Zdeněk ; Hovorka, Oldřich ; Fučík, Vladimír ; Borovičková, Lenka ; Maloň, Petr ; Čeřovský, Václav
Six new heptadecapeptides were isolated and synthesized by standard Fmoc solid phase peptide synthesistogether with several structural analogues. Antimicrobial activities were correlated with the peptides hydrophobicity, hydrophobic moment, alpha-helicity and HPLC retention time.
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