Národní úložiště šedé literatury Nalezeno 97 záznamů.  začátekpředchozí87 - 96další  přejít na záznam: Hledání trvalo 0.01 vteřin. 
Vernix caseosa and its newly discovered nonpolar lipids
Háková, Eva ; Míková, Radka ; Vrkoslav, Vladimír ; Doležal, A. ; Plavka, R. ; Cvačka, Josef
The aim of this work is a description of newly, so far undescribed nonpolar lipids, which are components of vernix caseosa.
Development and applications of ionization techniques in ambient mass spectrometry
Rejšek, Jan ; Vrkoslav, Vladimír ; Cvačka, Josef
Ambient mass spectrometry is a sharply developing subject area. Desorption electrospray ionization (DESI) and desorption atmospheric pressure photoionization (DAPPI) are two types of ionization techniques in the ambient mass spectometry. Herein, they were investigated in two research areas: (1) TLC analysis. (2) Localization of insect defense glands.
Sborník 14. ročníku Školy hmotnostní spektrometrie
Cvačka, Josef ; Vrkoslav, Vladimír
Sborník plných verzí přednášek zaměřených na základy hmotnostní spektrometrie, ionizační techniky, hmotnostní analyzátory, spojení hmotnostní spektrometrie a chromatografie, kvantifikaci, proteomiku, metabolomiku a interpretaci dat.
Hmotnostní spektrometrie v lipidomice
Cvačka, Josef
Podrobně jsou diskutovány současné postupy analytické chemie v lipidomice založené na hmotnostní spektrometrii a chromatografii. Jsou uvedeny příklady kvalitativních a kvantitativních metod včetně hmotnostně-spektrometrického zobrazování.
Anovel defensin from the mucus of the wood wasp Xiphydria camelus
Monincová, Lenka ; Fučík, Vladimír ; Voburka, Zdeněk ; Cvačka, Josef ; Čeřovský, Václav ; Šrůtka, P.
We have isolated a novel defensin from the mucus of the wood wasp Xiphydria camelus. The Edman degradation in 50 cycles revealed a peptide sequence similar to other insect defensins. The complete 55 amino acid residues sequence of X. camelus defensin was proposed based on the mass spectrometry analysis and by the deduction based on the comparison with the sequences of other hymenopteran defensins. The defensin was tested against both Gram-positive and -negative bacteria.
Imaging mass spectrometry of cuticular lipids of Drosophila melanogaster
Kaftan, Filip ; Vrkoslav, Vladimír ; Cvačka, Josef ; Svatoš, A.
The spatial distribution of cuticular lipids on the surface (head, thorax, abdomen and wings) of Drosophila melanogaster flies has been imaged and studied using MALDI-TOF mass spectrometry and scanning electron microscopy.
Methodology of lipid analysis of vernix caseosa using MALDI-TOF MS
Míková, Radka ; Vrkoslav, Vladimír ; Doležal, A. ; Plavka, R. ; Cvačka, Josef
This project is aimed to lipid analysis of intact molecules and optimization of MALDI-TOF MS method for these lipids.
Analysis of plant membrane lipids by RP-HPLC/HR-ESI-MS
Zábranská, Marie ; Vrkoslav, Vladimír ; Cvačka, Josef
Monogalactosyldiacylglycerols (MGDGs) and digalactosyldiacylglycerols (DGDGs) are glycolipids forming the major part of biological membranes in photosynthesizing organisms. The individual species differ from fatty acids linked to sn-1 and sn-2 possition on glycerol backbone. Complexity of their mixtures is increased by enzymatic or chemical oxidation of unsaturated species. The aim of this work was to develop and optimize RP-HPLC/HR-ESI-MS2 methods for analysis of oxidized and non-oxidized MGDGs and DGDGs in plant samples. MGDGs and DGDGs were isolated from Arabidopsis thaliana and Melissa officinalis.
Separation and identification of monogalactosyldiacylglycerols and digalactosyldiacylglycerols by RP-HPLC/ESI-MS
Krásová, Marie ; Cvačka, Josef
RP-HPLC/ESI-MS methods for analyzing complex mixtures of monogalactosyldiacylglycerols (MGDGs) and digalactosyldiacylglycerols (DGDGs) was developed. The methods allow separation and detection of more than 30 molecular species of MGDGs and more than 40 molecular species of DGDGs in plant samples.
New peptides with antimicrobial activity isolated from the venom of the bumblebee Bombus pascuorum (Hymenoptera: Apidae)
Ježek, Rudolf ; Janechová, D. ; Cvačka, Josef ; Slaninová, Jiřina ; Voburka, Zdeněk ; Hovorka, Oldřich ; Fučík, Vladimír ; Borovičková, Lenka ; Maloň, Petr ; Čeřovský, Václav
Six new heptadecapeptides were isolated and synthesized by standard Fmoc solid phase peptide synthesistogether with several structural analogues. Antimicrobial activities were correlated with the peptides hydrophobicity, hydrophobic moment, alpha-helicity and HPLC retention time.

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