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Cellulolytic fungi and their diversity on plant litter
Gálová, Diana ; Baldrian, Petr (advisor) ; Kolařík, Miroslav (referee)
Litter decomposition requires the presence of corresponding degradative enzymes, produced mainly by fungi. Forest soils show considerable spatial heterogeneity of distribution of these enzymes at diferent scales. Moreover, enzyme pruduction varies during the year, usually accompanied by the change in fungal community composition. In this work I examined if this spatial heterogeneity can be seen even at a scale of an individual leaf and whether the fungal community differs among enzyme activity hotspots and inactive parts of the leaves. Another goal was isolation of celulytic fungi from cellulose litterbags incubated on forest floor using particle filtration and dilution-to-extinction method. In a broadleaved forest dominated by oak leaves at different stages of decay were collected: senescent leaves on twigs, and leaves after 2, 10 and 22 months of decomposition. Ten leaves per season were taken for analysis of cellobiohydrolase activity over the leaf surface. Leaves were attachmed onto melted agarose plate and leaf surface was covered with low melting point agarose containing fluorescently labelled substrate. For each leaf a map of enzyme activity was created and area with the high and low enzyme activity was identified. From both sites a square of approx. 1 cm2 was cut out, DNA was extracted and fungal...
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Ecology of deadwood-associated fungi in the ecosystems of nature-like forests
Zrůstová, Petra ; Baldrian, Petr (advisor) ; Konopásek, Ivo (referee)
Dead wood plays an important role in forest ecosystems in the context of C dynamics, nutrient cycling, forest regeneration and biodiversity. Decaying wood sustains biodiversity by providing habitats and energy for fungi, bacteria, invertebrates, and many other organisms. Dead wood is resistant to decomposition and its decay is driven mainly by filamentous fungi. Community structure of wood- inhabiting fungi changes during decomposition, but the relationship between substrate quality and decomposer community is still poorly understood. This work studied fungal community composition with respect to tree species, stage of decay, volume and physico-chemical properties (such as pH, carbon and nitrogen content) of dead wood. Fungi were identified using next generation sequencing approaches - 454-pyrosequencing and Illumina MiSeq sequencing. Tree species, volume of dead wood (branches x logs) and stage of decay were the main variables affecting fungal community composition. Higher enzyme activities and content of fungal biomass indicate faster colonization of small branches than tree trunks by fungi. Fungal community composition, wood chemical properties and enzyme activities changed during decomposition. Both content of nitrogen and fungal biomass increased during decomposition. Enzyme activites peaked...
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Molecular biology of soil fungi participating in litter decomposition in forest ecosystems
Voříšková, Jana ; Baldrian, Petr (advisor) ; Gryndler, Milan (referee) ; Koukol, Ondřej (referee)
In forest ecosystems, substantial part of carbon enters soil in the form of plant litter. The decomposition of litter and soil organic matter represents an important process affecting nutrient cycling and carbon balance in soils. Fungi are considered the primary decomposers in terrestrial ecosystems due to the production of wide range of extracellular enzymes that allow them to attack the lignocellulose matrix in litter. Even if fungi represent key players in organic matter decomposition, the information about the structure and diversity of their communities is still limited and the roles of individual fungal taxa in forest soils remain unclear. This Ph.D. thesis focused on the characterization of fungal communities in forest soils and their potential to decompose plant litter. The method for in-depth analysis of complex microbial communities from environmental samples was established and used. In addition, single eukaryotic functional gene was analysed in soil for the first time at a depth that allowed reliable estimation of diversity. It was demonstrated that microbial community composition differs among horizons of forest soil profile. Despite similar diversity, significant differences in microbial community composition were observed between the DNA and RNA. Several microbial groups highly...
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Ecological roles of bacteria associated with plant litter
Tláskal, Vojtěch ; Baldrian, Petr (advisor) ; Seydlová, Gabriela (referee)
Leaf litter in temperate forests represents an important input of carbon into the soil. Main players in the decomposition of leaf litter are fungi and bacteria. While the role of fungi in litter decomposition was repeatedly addressed, there are just a few field studies where litter-associated bacteria were also considered. The aim of this work was to summarize available literature studying the structure and function of bacterial community during litter degradation. The nature of different possible substrates is discussed. Genetic approach is briefly outlined. Factors such as diversity, spatially distribution and abiotic factors that can influence community are also considered. It can be supposed that community composition changes with the change of litter chemistry and nutrient availability. The development of bacterial community might be driven by the decreasing availability of nutrients in litter. Labile compounds are utilised at the beginning of decomposition, while recalcitrant substrates are utilised later by specialist taxa. Members of the phyla Actinobacteria, Acidobacteria, Bacteroidetes and Proteobacteria seem to be dominant during decomposition. Different types of relationships between fungi and bacteria also most likely influence the composition of community. This review shows that the bacterial...
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Molecular biology of soil fungi participating in litter decomposition in forest ecosystems
Voříšková, Jana
In forest ecosystems, substantial part of carbon enters soil in the form of plant litter. The decomposition of litter and soil organic matter represents an important process affecting nutrient cycling and carbon balance in soils. Fungi are considered the primary decomposers in terrestrial ecosystems due to the production of wide range of extracellular enzymes that allow them to attack the lignocellulose matrix in litter. Even if fungi represent key players in organic matter decomposition, the information about the structure and diversity of their communities is still limited and the roles of individual fungal taxa in forest soils remain unclear. This Ph.D. thesis focused on the characterization of fungal communities in forest soils and their potential to decompose plant litter. The method for in-depth analysis of complex microbial communities from environmental samples was established and used. In addition, single eukaryotic functional gene was analysed in soil for the first time at a depth that allowed reliable estimation of diversity. It was demonstrated that microbial community composition differs among horizons of forest soil profile. Despite similar diversity, significant differences in microbial community composition were observed between the DNA and RNA. Several microbial groups highly...
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Mortality in the czech countries in the years 1920-1937 with emphasis on selected infectious diseases
Skalák, Zdeněk ; Fialová, Ludmila (advisor) ; Kurtinová, Olga (referee)
Mortality in the Czech countries in the years 1920-1937 with emphasis on selected infectious diseases Abstract The aim of this work is to analyze mortality rates in the czech countries in the years 1920-1937. We focus on a group of infectious diseases that had in this period in terms of cause of death still a high proportion. The rate of mortality due to infectious diseases is dependent on many aspects, such as the correct detection of the disease, effective vaccines and the level of medicine. It is these causes that brought about the sharp decline in mortality due to infectious diseases in the late 19th century. Hovewer, the First World War interupted this permanent decline and the newly created Czechoslovak state had to deal with relatively high mortality due to these diseases. The inter-war period saw recurrent epidemies of infectious deseases, nevertheless until the Second World War we can see the change in mortality due to causes. The infectious diseases are gradually replaced by modern diseases, especially cancers and diseases of the circulatory system. Key words: mortality, causes of death, infectious diseases, decomposition, classification of causes of death, medical discoveries, the level of health
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The Study of ZnO Crystal Growth in Supersaturated Alkaline Solution
Bannert, Tomáš ; Máca, Josef (referee) ; Chladil, Ladislav (advisor)
This thesis deals with the study of growth of zinc oxide crystals from supersaturated solutions using a scanning electron microscope and XRD methods. The work is divided into a theoretical and an experimental part. The theoretical part analyses issues related to the principle of operation of electrochemical cells with an emphasis on Ni-Zn batteries and phenomena limiting the life and efficiency of Ni-Zn batteries with a focus on phenomena that affect precipitation processes of zinc oxide crystals. It also analyses and compares the appropriateness of the methods that have been used to study the growth of the prepared zinc oxide crystals. The experimental part describes the preparation of a supersaturated solution, the process of each analysis and its evaluation. The results of each method are compared with the theoretical assumptions and among themselves. The experimental part also contains a chapter dealing with the design of in situ X-ray diffraction analysis methods of the obtained zinc oxide crystals.
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Decomposition and Forecast in Time Series
Šramková, Kristína ; Holec, Tomáš (referee) ; Bednář, Josef (advisor)
Bachelor thesis is focused on statistic methods for analysis and modelling time series. In particular decomposition techniques, which decompose time series into particular components and model trend component to predict future values. First part of thesis is focused on summary of essential knowledge of regression, basic properties of time series and their decomposition models. Hereinafter are discussed models of trend curves. In conclusion the mentioned methods are applied on real data and its modeling in software Statistica.
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Effect of surface electrode type on quality of decopmposition of surface EMG signal
Strusková, Edita ; Otáhal, Jakub (advisor) ; Michalec, Radim (referee)
Title: Effect of surface electrode type on quality of decomposition of surface EMG signal Objectives: The aim of this thesis is to evaluate the effect of surface electrode type, which was used for EMG record, on the quality of decomposition of surface EMG signal using decomposition software EMGlab. Methods: The form of the thesis is an experimental essay. It was detected an EMG signal from one healthy volunteer during mild cyclic contraction. It was used three different types of electrodes (standard surface electrode, tetrode, tetrode with saw-off spikes) for EMG signal detection. These signals were decomposed in program EMGlab. The results were processed in program MS Excel, compared with each other and graphically displayed. Results: The measurement verified a hypothesis which claimed that the used type of electrode affects the quality of automatic decomposition. The best results of automatic decomposition provide the signal gained from the standard surface electrode. Key words: action potential, decomposition, surface electrodes, EMG signal, tetrode, electromyography
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Analysis of Mortality Development in Russia using various decompositon methods
Kocová, Markéta ; Hulíková Tesárková, Klára (advisor) ; Burcin, Boris (referee)
Analysis of mortality development in Russia using various decomposition methods Abstract The aim of this thesis is to analyze and evaluate mortality development in Russia in last 50 years by using various decomposition methods. The first part presents the method of decomposition of the difference between two demographic indicators (E. Kitagawa's method or methods from E. Arriaga, R. Pressat and J. Pollard). In the second part mortality development in Russia is analyzed by using methods that decomposed the value of demographic indicators in a given year. Mortality is divided into senescent and background component by using the Gompertz-Makeham formula and by using the logistic model. Afterwards, avoidable and unavoidable mortality and mortality due to endogenous and exogenous causes of death is analyzed. Hypotheses, set out in the introductory chapter, are verified by using different decomposition methods. Using multiple methods of decomposition enable to obtain a more complex view of the evolution of mortality in the observed period so that could be viewed from multiple perspectives and identify specific population trends in Russia. Keywords: mortality, Russia, decomposition, senescent and background mortality, avoidable mortality, endogenous, exogenous, causes of death, mortality crisis
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