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Evaluation methods of government spending on healthcare in the context of life value
Dryzgalovich, Anton ; Vítek, Leoš (advisor) ; Ochrana, František (referee)
The Diploma thesis is focused on analysis of widespread theoretical and methodical techniques of government spending effectiveness evaluation. Emphasis is as well put on advantages and disadvantages clarification of particular methods, their mutual relations and on possible result interpretation error sources. In the theoretical part are suggested different modifications of traditional techniques, which extends usage abilities of those methods. Practical application is intentionally demonstrated on problematic healthcare area, since its controversy allows to better display different ways of real analytic tasks solving. The second chapter contains alternative evaluation approach suggestion in form of computer model of human life economic value estimation. Because of mutual synthesis of different methods, text provides to a reader a complex evaluation system with wide spectrum of usability.
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Analysis of the composition of selected probiotic products by PCR-HRM
Tomanová, Barbora ; Španová, Alena (referee) ; Trachtová, Štěpánka (advisor)
This work was focused on the detection of probiotic bacteria in four different probiotic products (probiotic cream, probiotic tampons, oral probiotics and soy beverages with probiotics). The viability of the bacteria contained in the products was verified. Complex matrices of the products were used to isolate DNA in a quality suitable for the PCR method, followed by identification of the declared bacterial genus and species. Amplification was achieved with conventional PCR and real-time PCR, genus- and species-specific primers were used. Bacteria, of the genus Lactobacillus and Bacillus and bacterial species Lactobacillus pentosus, Lactobacillus rhamnosus, Lactobacillus fermentum and Lactobacillus gasseri, were proven to be within the products. Subsequently, the DNA from mixed bacterial species in the probiotic tampon were distinguished using PCR-HRM. Five sets of primers were used to test this. Two sets of primers (primers P1V1, P2V1 and V1F-HRM, V1R-HRM) were evaluated as the most suitable for resolution.
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Spatial Decomposition for Differential Equation Constrained Stochastic Programs
Šabartová, Zuzana ; Mrázková, Eva (referee) ; Popela, Pavel (advisor)
Rozsáhlá třída inženýrských optimalizačních úloh vede na modely s omezeními ve tvaru obyčejných nebo parciálních diferenciálních rovnic (ODR nebo PDR). Protože diferenciálních rovnice je možné řešit analyticky jen v nejjednodušších případech, bylo k řešení použito numerických metod založených na diskretizaci oblasti. Zvolili jsme metodu konečných prvků, která umožňuje převod omezení ve tvaru diferenciálních rovnic na omezení ve tvaru soustavy lineárních rovnic. Reálné problémy jsou často velmi rozsáhlé a přesahují dostupnou výpočetní kapacitu. Výpočetní čas lze snížit pomocí progressive hedging algoritmu (PHA), který umožňuje paralelní implementaci. PHA je efektivní scénářová dekompoziční metoda pro řešení scénářových stochastických úloh. Modifikovaný PHA byl využit pro původní přístup prostorové dekompozice. Aproximace diferenciálních rovnic v modelu problému je dosaženo pomocí diskretizace oblasti. Diskretizace je dále využita pro prostorovou dekompozici modelu. Algoritmus prostorové dekompozice se skládá z několika hlavních kroků: vyřešení problému s hrubou diskretizací, rozdělení oblasti problému do překrývajících se částí a iterační řešení pomocí PHA s jemnější diskretizací s využitím hodnot z hrubé diskretizace jako okrajových podmínek. Prostorová dekompozice byla aplikována na základní testovací problém z oboru stavebního inženýrství, který se zabývá návrhem rozměrů průřezu nosníku. Algoritmus byl implementován v softwaru GAMS. Získané výsledky jsou zhodnoceny vzhledem k výpočetní náročnosti a délce překrytí.
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Study of resonance and threshold effects on simple two-channel model
Kocák, Jakub ; Čížek, Martin (advisor) ; Kolorenč, Přemysl (referee)
In this thesis we study simple one-dimensional two-channel scattering model where pointlike coupling between channels is provided by the delta potential. The scattering task can be completely solved analytically. The solution of the Lippmann-Schwinger equation leads to improper scattering eigenvectors, consequently to scattering S matrix elements and eigenphases. We study how the setting of parameters affects threshold and resonant behaviour (presence, position, width) and the mutual relationship between resonances and poles of the S matrix in complex k-plane. Then we apply projection- operator formalism to model with resonance and the on-shell T matrix is separated into the orthogonal, direct and resonant term. We discuss how choice of subspace of quasi-bound states effects the separation. Powered by TCPDF (www.tcpdf.org)
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Comparision of FEM models of joints
Hlaváč, Martin ; Hrůza, Václav (referee) ; Pokorný, Přemysl (advisor)
This thesis deals with the comparison of alternative models of the simplified joints used in the FEM models of complex models with detailed joint details. This is essentially a screw connection with dowel bolt and standard bolt, weld connection "T" and overlap weld connection. Individual connections are compared in the simulation results of matching models.
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Non - covalent interactions in fundamental biological processes
Klusák, Vojtěch ; Rulíšek, Lubomír (advisor) ; Burda, Jaroslav (referee) ; Šponer, Jiří (referee)
Charles University in Prague Faculty of Science Department of Physical and Macromolecular Chemistry Non-covalent interactions in fundamental biological processes Doctoral Thesis Abstract Mgr. Vojtěch Klusák Supervisor: Mgr. Lubomír Rulíšek, CSc. Institute of Organic Chemistry and Biochemistry AS CR Center for Biomolecules and Complex Molecular Systems Praha 2010 2 3 Universita Karlova v Praze Přírodovědecká fakulta Katedra fyzikální a makromolekulární chemie Nekovalentní interakce v základních biologických procesech Souhrn disertační práce Mgr. Vojtěch Klusák Školitel: Mgr. Lubomír Rulíšek, CSc. Ústav organické chemie a biochemie AV ČR Centrum biomolekul a komplexních molekulárních systémů Praha 2010 4 5 Introduction Understanding inter- and intra-molecular interactions is the key for our insight into the properties of the biomolecular systems which, in turn, maintain and govern virtually all the processes in biology. Attempts to draw the structure-function relationship at the atomistic or electronic level bring us quite often beyond experimental resolution and capabilities. Modern tools of computational chemistry enable us to focus with satisfactory degree of reliability on the details of the studied process, gather additional (often complementary) information and ascribe particular structural features to...
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Catalytic and Electronic Properties of Redox-Active Metalloenzymes and Transition-Metal Complexes: Insights from the Computational Chemistry.
Srnec, Martin ; Rulíšek, Lubomír (advisor) ; Himo, Fahmi (referee) ; Papai, Imre (referee)
Charles University in Prague Faculty of Science Department of Modeling of Chemical Properties of Bio- and Nanostructures Catalytic and Electronic Properties of Redox-Active Metalloenzymes and Transition- Metal Complexes: Insights from the Computational Chemistry Dissertation Thesis Abstract RNDr. Martin Srnec Supervisor: Mgr. Lubomír Rulíšek, CSc. Institute of Organic Chemistry and Biochemistry AS CR Center for Biomolecules and Complex Molecular Systems Praha 2010 Introduction Metals and their ions play a key role in maintaining life. They frequently promote protein folding, the stabilization of protein scaffolds, enzymatic activity, energy conversion, intra- and intercellular signals etc. Several decades ago, metalloproteins were considered as a rather small group of proteins. Nowadays, one-third of enzymes are estimated to contain one or more metal ions, whose presence is crucial for their enzymatic functionality. Metalloenzymes participate most often in the catalysis of difficult chemical reactions (e.g. the hydroxylation of methane, decomposition of H2 into protons and electrons, N2 and O2 bond cleavage and many other examples), in oxidation-reduction and electron-transfer reactions, and in the catalysis of spin-forbidden reactions, where relativistic effects (i.e. spin-orbit coupling) are necessary....
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Side-chain Side-chain Interactions in Proteins
Berka, Karel ; Hobza, Pavel (advisor) ; Rulíšek, Lubomír (referee) ; Otyepka, Michal (referee)
Charles University in Prague Faculty of Science Department of Physical and Macromolecular Chemistry Side-chain Side-chain Interactions in Proteins Doctoral Thesis Abstract RNDr. Karel Berka Supervisors: Prof. Ing. Pavel Hobza, DrSc., FRSC RNDr. Jiří Vondrášek, CSc. Institute of Organic Chemistry and Biochemistry AS CR Center for Biomolecules and Complex Molecular Systems Praha 2009 2 Introduction Proteins are the most versatile and useful molecules in the cellular arsenal. They are the best catalysts the nature knows. Proteins cover the biggest amount of the cellular functions with range from metabolism and signaling through cell architecture to DNA replication. Variations of their structure and functions are amazing. And yet, they are built from simple building blocks - amino acids. Each amino acid has many possibilities of interactions with its neighborhood and the sequential context manifested through these possibilities is the main reason for the structure variability. The experimental investigation of the character and relative strength of interactions between amino acid residues is difficult. On the other hand, theoretical chemistry methods and techniques of are well suited for such task. They can provide useful information about structure, stability and nature of these interactions. The aim of the...
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Travelling salesman problem – delivery of postal items
Říha, Vojtěch ; Borovička, Adam (advisor) ; Fábry, Jan (referee)
The aim of this thesis is to propose the process of delivering postal items by using general knowledge of the travelling salesman problem. The first part briefly describes the historical evolution concentrating on solving real problems. The second part describes the travelling salesman problem and the multiple travelling salesman problem. Emphasis is placed on heuristic algorithms, primarily on the nearest neighbour algorithm. Various modifications of the travelling salesman problems are also mentioned. In the part of the application a real problem of delivering postal items in a given area is handled in order to minimize the total time spend on delivery. For this specific type of problem a mathematical model is design as well as its possible modification for minimizing the earliest possible time of vehicle's arrival to the village. For the optimization, computer software MPL and Lingo are used. Interim results of these calculations are described in detail. Due to the complexity of the calculation the modified nearest neighbour algorithm is suggested. Its process is programmed in MATLAB software and is described in detail. In this thesis the intention of reducing the coefficient of the limit in MATLAB is also presented and then these results are applied. In the conclusion the results of heuristic algorithms are carefully analysed and compared with interim results of MPL.
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