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CO2 reduction with algae
Naider, Jakub ; Rebej, Miroslav (referee) ; Vondál, Jiří (advisor)
The aim of this final thesis is the process of algae cultivation their use for carbon dioxid reduction. The main objective of this work is the mass and heat balance of the photobioreactor and the design of a temperature control system. Within the research work, an overview of certain types of bioreactors and photobioreactors, the current situation around the climate and the impact of the cultivation of algae on carbon dioxide emissions. The model for temperature control and mass transfer of a plate photobioreactor is created in this work.
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Use of corneal endothelium and amniotic membrane for transplantation purposes.
Šmeringaiová, Ingrida ; Jirsová, Kateřina (advisor) ; Netuková, Magdaléna (referee) ; Čejková, Jitka (referee)
Part I: Endothelial cells form the posterior layer of the cornea and are important for maintaining its transparency. Dysfunctional endothelium can only be restored by transplantation. The global shortage of donor corneas requires the search for alternative treatments. The preparation of the graft by tissue engineering methods is complicated by low proliferative capacity of endothelium. To date, no endothelium-specific marker has been defined and the existence of endothelial stem cells has not been confirmed yet. We have prepared a protocol for culturing endothelial cells from research-grade tissue - corneoscleral rims obtained after transplantation or corneas excluded from the transplant process. We monitored localization of selected proteins, including stem cell markers, in native tissue and in primary cell cultures. We prepared up to 6.4 cm2 of endothelium from one cornea/rim, which had cellular features comparable to the native endothelium. This approach can increase the amount of endothelium for research or transplantation purposes. Using indirect immunohistochemistry, we showed that none of the previously proposed endothelial molecular markers is specific for these cells. We detected the expression of stem cell markers throughout the endothelial layer. In the porcine cornea model, we monitored...
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Využití meziřádkové kultivace půdy při pěstování kukuřice v zemědělském podniku
Ambrož, Jaroslav
This master thesis is focused on the use of inter-row cultivation in silage maize. The practical part of the work was realized in the agricultural enterprise, AGRO Zvole. The experiment was aimed at comparing the yield of maize for silage without cultivation, using inter-row cultivation without fertilization and using inter-row cultivation and liquid fertilizer. The results in 2018 demonstrated the effectiveness of using inter-row cultivation with using liquid fertilizer.
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Kultivace Vitis vinifera v podmínkách in vitro a převod do in vivo podmínek
Bechyňová, Soňa
I examined in vitro behaviour three table varieties of grapes and Vitis rootstock Craciunel 2 during different micropropagation stages and different rhizogenezis media composition in vitro conditions. The experiment held the place in Lednice na Morave in 2018. So far have been achieved best results of 90% rooting success with DKW medium with the addition of growth regulators at this concentration of 0,2 mg.l-1 NAA + 0,2 mg.l-1 IAA + 1 mg.l-1 IBA. Multiplication protocol suitable for use in horticultural practice were optimized for Craciunel 2. Different media factors affect rooting have been determined.
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Control of algal bioreactors
Rek, David ; Naď, Martin (referee) ; Máša, Vítězslav (advisor)
The bachelor’s thesis discusses the matter of cultivation of microalgae in an artificially altered environment – bioreactor. The aim of the thesis is to determine and present the factors influencing the growth of microalgae and to find means of automated regulation of such factors. Nowadays, the majority of bioreactors are laboratory ones and are mostly being operated manually. The recent increase in demand for this technology results in many pilot-plant tests with a wide variety of microalgae. Tests such as these will be required to be automated to serve properly in future research. The thesis is based on professional literature and articles. The first part is to familiarize the reader with the wide number of potential applications of microalgae technology in various fields, such as the food industry, water treatment or separation of CO2 from ambient air or waste gas. The next segment presents the factors influencing the growth of microalgae and lastly the system of required equipment and sensors for automated operation is described. The bachelor’s thesis summarizes the important information necessary for the construction and unattended operation of microalgae bioreactor with respect to maximalization of productivity.
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Possibilities of ex situ protection of rare and endangered plant species
Vítová, Jana ; Münzbergová, Zuzana (advisor) ; Dostálek, Tomáš (referee)
The aim of the bachelor's thesis is to present the possibilities, significance and possible problems of ex-situ protection for the future conservation of biodiversity of plants on Earth. In the introductory part of the thesis, the individual processes and programs of ex situ protection are described, with the help of literature search. At the same time, information on international cooperation in the field of seed banks and botanical gardens is presented, which, through their activities and mutual cooperation, create rescue programs to secure as many samples of plant material for possible future use. The work also mentions international plant databases, which provide the accurate and detailed information about the preserved material of rare and endangered plant species for the public. Further in the thesis, the currently known problems of ex situ protection with their impacts on the collected and stored material are mapped. Keywords Ex situ conservation, threatened plants, Ministry of the Environment, database, seed dormancy, inbreeding, outbreed depression, cultivation, seed bank, botanical gardens, tissue cultures, in vitro, genetic drift, mutation, hybridization, wildlife conservation, endangered species, threatened species
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Characterization of yeast and algae metabolites by instrumental techniques
Kurowska, Klára ; Márová, Ivana (referee) ; Němcová, Andrea (advisor)
The presented diploma thesis deals with the analysis and production of selected microbial metabolites of the yeast Rhodotorula kratochvilovae in comparison to various species of microalgae. The theoretical part summarizes the knowledge about the conditions for optimal growth and production of secondary metabolites. Analytical methods, by which metabolites were quantified and evaluated, were also described. In the experimental part of this thesis, the amount of accumulated biomass, exoglycolipids, intracellular lipids, phycobiliproteins and carotenoids according to various nutrient sources in the culture medium was studied. Specifically, the yields of these metabolites were investigated in the utilization of various sources of nitrogen (yeast extract, urea, ammonium sulfate, ammonium chloride and potassium nitrate), carbon (glucose, mannose, xylose, glycerol and lactose) and at variable C / N ratios (20; 40; 70; 100 and 120: 1) ideally selected sources of observed nutrients in partial experiments. The last part of the experiment was focused on comparison of the analysis of metabolites of autotrophic algae (chlorophylls, phycobiliproteins and lipids).
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Optimization of cultivation of carotenogenic yeasts on mixed waste substrates
Holub, Jiří ; Mikulíková, Renata (referee) ; Márová, Ivana (advisor)
The master thesis addresses the issue of cultivation of selected strains of carotenogenic yeasts on waste materials of the food industry using a laboratory bioreactor. Carotenogenic yeasts are able to produce highly valuable metabolites during cultivation, which are located predominantly in the lipid part of the cells. Particularly, they are carotenoids, ergosterol, coenzyme Q and fatty acids. The thesis is divided into two main parts, the theoretical part and the practical part. The theoretical part describes individual yeast strains, types of waste materials, produced metabolites and methods of their analysis. The experimental part deals with the processing of waste materials of the food industry, specifically animal fat, whey and spent coffee grounds into the form of substrates usable as nutrition sources for yeast cultivation. Furthermore, cultivations focused on the recovery of the monitored metabolites and their analysis by using HPLC/PDA and GC/FID assemblies were studied as well. The yeast strains Rhodotorula mucilaginosa (CCY 19-4-6), Rhodotorula kratochvilae (CCY 20-2-26), Rhodosporidium toruloides (CCY 062-002-001), Sporidiobolus pararoseus (CCY 19-9-6) a Cystofilobasidium macerans (CCY 10-1-2) were used in this work. As one of the best producing strains Sporidiobolus pararoseus (CCY 19-9-6) was found, which achieved very high productions of carotenoids, coenzyme Q and ergosterol.
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Use of algae for production of biomass and bioproducts
Slonek, Jaroslav ; Máša, Vítězslav (referee) ; Brummer, Vladimír (advisor)
Submitted bachelor thesis is focused on description of use of algae for biomass and bioproducts production. The first part of the thesis is devoted to the taxonomic classification of algae and a basic summary of the characteristics of the different algal groups. The next part is aimed at bioproducts, which are obtained by algae cultivation and possibilities of further processing for energy purposes are also described. As for the cultivation, the emphasis is on modern methods in this area, i.e. on the possibility of using waste products in the culture process. In the next part, technological parameters of cultivation are discussed, for instance effects of technological parameters on growth and composition of biomass and production of desired products. The next chapter summarizes types of used culture bioreactors in which the growth of biomass occurs in a controlled environment. The information obtained and collected in the thesis is further discussed and summarized in the conclusion.
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Live food organisms for fish - an overview
MILLEROVÁ, Adéla
Feeding organisms are an important part of the fish's nutrition, they are used not only when foddering fish larvae, but also as a supplementary feed for adult fish. The addition of animal and vegetal ingredients in the feed of fish has a proven effect on their growth, reproduction, condition and the intensity of their coloration. To the group of feeding organisms used in fishkeeping belong protozoans, rotifers, crustaceans, nematodes, oligochaetes, molluscs and insects. A specific group of feeding organisms are algae and cyanobacteria, which are used not only as a supplementary feed for aquarium fish, but also as a food for some feeding organisms, for example rotifers, cladocera and copepods. The individual chapters of the thesis contain information about the usage of these specific organisms in fishkeeping, basic characteristics (size, occurrence, eventually also reproduction), breeding in home conditions (inclusive the list of needed things, advantages, disadvantages and the costs of breeding), also the method of obtaining and preservation of this specific type of nutriment.
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