|
Analysis of the behavior and decision-making of the Dutch auction and the ascending auction with one price of homogeneous product
Svatošová, Ludmila ; Hudík, Marek (advisor) ; Svoboda, Miroslav (referee)
This work deals with the behavior and decision making of players in the English auction with homogeneous goods. To analyze the behavior and decision making, laboratory experiment was conducted. It contains the description and analysis of laboratory experiment, which was carried out for the purposes of this thesis. The fundamental question that this work tries to answer is whether the frequency of the optimal price varies according to auction environment and whether, in all environments the choice of optimal strategy prevails. The experiment used three types of environments, each of them is characterized by a different probability distribution of values. Analysis produced as follows: first in each auction round the optimal prices was determineds and then it was compared to the price actually achieved. Based on this analysis it was found that the frequency of optimal price choices doesn't differs significantly depending on the environment. However , the optimal price was achieved only in very few rounds of the auction.
|
|
Analysis of the system of assessment of employees in PROF SVAR s.r.o.
Novotná, Barbora ; Jarošová, Eva (advisor) ; Pauknerová, Daniela (referee)
The thesis is dealing with the system of assessment of employees in a firm developing and producing trailer towing devices. The aim of this thesis is to suggest optimization arrangements and eventually recommodations for the improvement of this area of the company's human resource management. The theoretical part of the thesis characterizes the question of work motivation, system of assessment of employees, allocation of manpower, remuneration and further training and development of employees. The practical part of the thesis characterizes these areas of the personnel work in the company. Empirical research then discovers attitude of the employees towards the company's system of assessment. Results of the questionnaire examination are used for suggestion of optimization arrangements for the improvement of the system of assessment and strengthen work motivation and satisfaction of the company's employees.
|
|
Sequential injection chromatography
Chocholouš, Petr ; Solich, Petr (advisor) ; Polášek, Miroslav (referee) ; Blešová, Marie (referee)
This work brings extending of applications of sequential injection analysis in area of pharmaceutical analysis. It is focused on integration of monolithic columns into SIA system that enables simultaneous determination of few analytes in samples with matrix (biologic samples) or simple mixtures (pharmaceutical preparations). A new created method was called Sequential Injection Chromatography (SIC) and represents a new generation of flow methods. Integration of short monolithic column with low back-pressure leads into creation of low pressure flow separation method. Commercially produced monolithic columns with reverse phase sorbent C18 are suitable for determination of active compounds and adjuvants in pharmaceutical preparations. This enables fast determination of samples without or only with simple off-line pre-treatment of sample. Employment of various lengths of monolithic columns used in SIC was studied. First experimental work (appendix I) was first SIC experiment. The aim of it was trouble-free connection of monolithic column with SIA manifold and application on determination of pharmaceutical preparation Diclofenac HBF emulgel (ointment contains diclofenac nitrate, methylparaben and propylparaben). All parts of system were optimized for higher work pressure. Sample of ointment was...
|
|
The innate immunity and circulating monocytes - their significance and function in pathogenesis of coeliac disease.
Němečková, Iva ; Daňková, Pavlína (advisor) ; Palová Jelínková, Lenka (referee)
8 Abstract Introduction: Celiac disease is indentified as the loss of oral tolerance to gluten, it is an organ-specific autoimmune disease in which both, adaptive and innate immunity participate. Monocytes are important part of immune system; they have many functions and express very diverse membrane receptors including Toll-like receptors (TLRs). TLRs are involved in the innate immune response, specifically TLR2 and TLR4 are crucial for recognition of bacterial components and TLR7 recognizes virus's ssRNA. Monocytes also produce prolaktin (PRL), which acts as a cytokine that modulates immune responses. To clarify the role of innate immunity and circulating monocytes in pathogenesis of celiac disease, we focused on changes in expression of selected Toll-like receptors (TLR2, TLR4, TLR7), prolactin, some pro- a anti-inflammatory cytokines (TNF-α, IL-6, IL-12, IL-10). We monitored the influence of the SNP - 1149 G/T on the expression of PRL mRNA. Another objective of this work was the introduction and optimization of in vitro methods for cultivation and stimulation of peripheral monocytes. Material and Methods: This pilot study includes 21 patients with celiac disease and 40 healthy controls. For determination of mRNA levels of the studied genes we isolated RNA from monocytes that were isolated by...
|
|
Superparamagnetic nano- and microparticles with hydrophilic surfaces
Babič, Michal ; Horák, Daniel (advisor) ; Šňupárek, Jaromír (referee) ; Šafařík, Ivo (referee)
This work deals with a preparation of superparamagnetic nano- and microparticles with hydrophilic surfaces for bioapplications. The wok is divided into three parts in consonance with experimentally solved problem. First part describes a choice and an optimalization of synthesis of iron oxides nanoparticles with appropriate toxicological, morphological and physico-chemical properties, which surface can be post synthetically modified. Maghemite - γ-Fe2O3 particles were prepared by consequent oxidation of mangnetite - Fe3O4 as an initial substance for a preparation of materials for diagnostics and separations. A conventional alkaline coprecipitation method of magnetite preparation was modified to produce nanoparticles with narrowed size distribution without use of surfactants during their synthesis. Prepared maghemite nanoparticles were cca. 6 nm in diameter and their saturation magnetization was Ms ~ 70 A·m2 ·kg-1 . Such observed value is far higher in comparison with the state of the art and argues thus a proposition the Ms depends not on the preparation method, but only on the nanoparticles size. Maghemite nanoparticles morphology was evaluated by picture analysis of SEM and TEM micrographs, hydrodynamic size and zetapotential was measured with DLS. The structure of the maghemite was confirmed with...
|
|
Optimalization and validation of HPLC method for the determination of diclofenac sodium and its degradation product in tablets
Birková, Agáta ; Matysová, Ludmila (advisor) ; Chocholoušová Havlíková, Lucie (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Agáta Birková Supervisor: PharmDr. Ludmila Matysová, Ph.D. Title of Diploma Thesis: Optimization and validation of HPLC method for the determination of sodium diclofenac and its degradation product in tablets This diploma thesis deals with the optimisation and the validation of the HPLC method determination of sodium diclofenac (DF) and its degradation product 1-(2,6-dichlorphenyl)-indolin-2-on (DPI) in tablets. DF is a non-steroidal anti-inflammatory drug with analgesic, antipyretic and anti-inflammatory effects. The degradation product DPI has been produced in formulations after a long-term storage, especially after exposure to light or heat. The method development was based on a method created by the Pharmaceutical Faculty of Charles University for the determination of DF, its degradation product and preservatives in topical emulgel and the method was optimised for determination of DF and DPI in tablets. The monolithic column Chromolith® Performance RP-18e (100×3 mm, Merck) and flurbiprofen as an internal standard were chosen. The mobile phase was prepared of methanol and aqueous solution of phosphoric acid (pH 2,5) in the ratio of 65:35 and the flow rate of 1 ml/min. An UV detector...
|
|
Secondary metabolite production in actinomycetes causing and suppressing common scab of potatoes
Komžák, Ondřej ; Bosáková, Zuzana (advisor) ; Slaninová Kyselková, Martina (referee)
This diploma thesis focused on screening for bacterial pathogens and antagonists suppressing common scab mainly caused by Streptomyces scabiei. Common scab affects some agricultural crops causing significant economical losses. Bacterial strains, mostly streptomycetes, were isolated from potato rhizosphere because they belong to most important producents of secondary metabolites and the causative agents of the disease are also members of this genus. The isolated bacteria were characterised by PCR amplification and sequencing of 16S rRNA gene to reveal their phylogenetic relationships. The ability of isolated strains to suppress growth of Streptomyces scabiei was tested by a simple co-cultivation experiment. The strains were tested by PCR for presence of specific genes for biosynthesis of thaxtomin A, a common virulence factor found in all described pathogens causing symptoms of this disease on the surface of affected tubers. Genes for synthesis of thaxtomin belong to pathogenicity island. Standard of phytotoxin thaxtomin A was used to optimize its analysis by mass spectroscopy for further in vivo and in vitro experiments. Phylogenetic analysis of strains harboring one of the genes necessary for thaxtomin A biosynthesis supported the hypothesis of sharing the pathogenicity island by horizontal gene...
|
|
Heterologous expression of human NADPH:cytochrome P450 reductase
Mazurová, Martina ; Martínek, Václav (advisor) ; Ingr, Marek (referee)
Study of carcinogenesis is associated with study of xenobiotics metabolism, which is topic studied in our laboratory. Mixed-function oxygenase system (MFO system) is significantly contributing to the metabolism of xenobiotics. Pure recombinant proteins participating in MFO system are frequently utilized in in vitro metabolic experiments. The heterologous expression method is often used to obtain the pure recombinant enzymes. Heterologous expression was employed to prepare human NADPH:cytochrome P450 oxidoreductase. This membrane enzyme reduces cytochrome P450 and enables its catalytic activity. Vectors with synthetic gene for human NADPH:cytochrome P450 oxidoreductase based on pUC19 and pET22b plasmids were prepared and verified. Recombinant protein was produced in E. coli BL21-Gold and E. coli BL21-CodonPlus-RIL cells. Both cell strains produced high levels of the protein; however the major part of the protein was present predominantly in inclusion bodies. Expression conditions were therefore optimized to obtain higher yields of native protein bound in bacterial membrane fraction. [In Czech]
|
|
Pěvecký formant: Předběžné výsledky vyhodnocení snímků z magnetické rezonance a akustických záznamů při fonaci zpěváků
Švec, J. ; Herbst, C. ; Havlík, R. ; Horáček, Jaromír ; Krupa, P. ; Lejska, M. ; Miller, D.G.
Singerś formant is a desirable quality in operatic singing. This study investigates the strategies of two male operatic singers used for achieving the singerś formant. The subjects were asked to produce voice both in naïve and operatic singing quality at the same fundamental frequency for two different vowels [a] and [e]. Changes in the shape of the vocal tract were investigated using magnetic resonance imaging (MRI), and differences in acoustic voice quality were analyzed using sound spectrography. In operatic voice quality the MR images revealed a) lowered larynx, b) raised soft palate, c) flattened tongue and d)straightened spine. Clustering and lowering the formant frequencies was seen in the spectrograms but the strategy was different for the two vowels and the two singers. The data suggest that different anatomical dispositions in singers lead to different strategies for optimizing the voice quality.
|
|
Multiple sequence alignment
Ferenc, Matej ; Pangrác, Ondřej (advisor) ; Bálek, Martin (referee)
Title: Multiple Sequence Alignment Author: Matej Ferenc Department: Department of Applied Mathematics Supervisor: RNDr. Ondřej Pangrác, Ph.D. Supervisor's e-mail address: pangrac@kam.mff.cuni.cz Abstract: In this work we study multiple sequence alignment problem, for aligning protein or DNA sequences. There is a lot of ways how to solve this problem. Some of them are optimized for speed, while others are optimized for quality of the produced alignment. We implement two methods - progressive and iterative, which are based on creating a phylogeny tree and align sequences according to it. We will also provide a few distance methods. Our aim is to compare the methods and their parameters for creating best alignments and to find out, when to use which methods. Keywords: bioinformatics, alignment, sequences
|
guest ::
