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Clinical biochemical indicators used in diagnostics of equine diseases
Puldová, Doubravka ; Ptáčková, Zuzana (advisor) ; Krejčířová, Romana (referee)
The thesis aims to compile overview of main biochemical indicators that are important in for diagnostics of equine diseases.
Clinical biochemistry is a medical discipline that uses biochemistry and pathobiochemistry for the determination of diagnosis based on activity changes of analytes observed. Laboratory evaluation of analytes has three phases: pre-analytical (preparation of patient to sampling, sampling and sample storage and transport), analytic (analysis) and post-analytical (evaluation of results, veterinarian also contributes in this part). Pre-analytial phase is the most time consuming and also the most error prone. The errors mostly occur during preparation of the patient, during sampling or sample transportation. The most analyzed materials for biochemical analysis are blood, urine and liquor.
Biochemical indicators are compound that are often influenced by the disease, therefore it is possible to use them for the diagnosis. Nitrogen metabolism indicators include proteins, urea, creatinine and ammonia. Enzymes (aminotransferase, gama-glutamyltransferase, glutamatedehydrogenase, lactatedehydrogenase, alkaline phosphatase, creatinkinase and sorbitoldehydrogenase are the main indicators of liver function (they contribute on the metabolism of another compounds) or of the fitness of horse. Glucose and lactate values indicate energetic metabolism, cholesterol and triglycerides indicate lipid metabolism. Water and electrolyte metabolism indicators are sodium, potassium and chlorides. The mineral profile is shown by calcium, magnesium and phosphorus.
The result of determination of analytes is compared with reference values that are not only species-specific but can be also influenced by age or sex. These values represent the range of the compound concentration in body fluid under physiological conditions. It is important to take the clinical symptoms into the account. Not all indicators that are usually indicated in human medicine or different animals are suitable for diagnosis of equine diseases. Majority of indicators diagnose equine-rare diseases (i.e. diabetes mellitus or hepatitis). Determinations of muscle enzymes and lactate are important for the fitness analysis.
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Monitoring of raccoon dog aktivity in Opava
Staněk, Petr ; Kušta, Tomáš (advisor) ; Ježek, Miloš (referee)
This work deals with invasive predator racoon dog (Nyctereutes procyonoides). It is a comprehensive work consisting of general information about its history and later spread to the whole Word, etology and biology as information collected on the basis of thousands of images and hundreds of videos from scout camera located close to natural burrow at Opava in the hunting district hunting club in Hlavnice. The emphasis here is primarily on the course of the day and night activities throughout the year, food preferences, coexistence with other kinds of beasts and ways of dealing with stressful situations. During the observation we were found that his peak of activity was among the sunrise and the second one lately after sunset. Cohabitation with badger is also possible. As for food preferences we realized that racoon dog did not prioritize any kind of food and eat everything what we gave to him.
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Effect of brassinosteroid and Atonik on growth of onion in different moisture conditions
Pařízková, Lada ; Doležalová, Jitka (advisor) ; Augustinová, Ludmila (referee)
Summary The aim of this study was to investigate the effect of synthetic brassinolide and Atonik, as a growth promoter supporting growth and development of plant that have been grown in different moisture conditions. For experiment were used two species of onion ('Alice'and'Lusy') which had been treated during the growth with synthetic brassinolide solution in concentrations 1.10-9 mol.l-1 and 1.10-11 mol.l-1 and 0.1% concentration of Atonik solution. For controlling was sown untreated variant. Average plant height and later diameter of the neck were observed and recorded during the experiment. Results showed that the product Atonik had in both variants positive influence on plant growth in deficit moisture conditions. Its effects have been later inconclusive or inhibitory. Brassinosteroid had positive effect on growth under optimal moisture conditions in "Lusy's" case and in stressful conditions in "Alice's" case. Brassinostreroid, unlike Atonik product, prevailed in neck diameter efficiency. In both cases, brassinosteroid had more positive effect on average yield in optimal moisture conditions. In deficit moisture conditions had Atonik product more positive effect on the yield of onion in both cases. To verify the results, it is necessary to repeat the experiment several time.
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Performance recording in Charolais beef herd
Jelínek, Petr ; Toušová, Renata (advisor) ; Petr, Petr (referee)
Summary
In this thesis I dealt with verification of performance of beef cattle Charolais Breed on farm: Chov Charolais spol. s r. o. Slabce. The main objective of my diploma work was to assess the growth abilities of calves Charolais breed from birth till weaning in relation to various factors, for instance: gender, order of calving, number of born calves during one calving, month and year of the birth on the respective farm. The relevant data was assessed for the period of years 2012 - 2015. In this period there was born 324 calves of which 162 bulls, 153 heifers and 9 still born calves. The assessment of calf's growth characteristics was related to the average birthweight, average gain from birth to 120 days, and average weight at the age of 120 and 210 days. For the evaluation of calf's growth characteristics was used data gained from the database of performance testing of beef cattle (KUMP) for the given period. Growth parameters related to selected factors were processed with the use of statistical software SAS 9.3 (SAS 9.3, 2011).
The average weight of young bulls at birth was 33,58 kg and average weight of heifers was 32,99 kg. The average weight of bulls at the age of 120 days was 183,66 kg and the average weight of heifers was 175,58 kg which was demonstrated to be statistically significant (P < 0,01). The average weight of bulls at the age of 210 days was 288,28 kg which is again higher than heifers with average weight of 264,21 kg (P < 0,05). To summarize, there was identified statistically significant difference of the gender influence in behalf of bulls.
After comparison of average weight at birth, at the age of 120 and 210 days separately for twins and an only child, there is obvious and also statistically significant finding (P < 0,01) that the observed twins are usually smaller at birth and their growth abilities are worse than that of only child. The average weight at birth for an only child is about 4,48 kg higher compared to twins. Then the average weight at the age of 120 days for an only child is about 38,68 kg higher than average weight of twings. Finally the average weight at the age of 210 days is about 58,26 kg higher for an only child.
Regarding to the influence of order of calving there was identified no significant effect to both average birth weight and weight at the age of 210 days. The only statistical significance was identified between order of calving and the average weight at the age of 120 days in 1st, 2nd, 5th, 7th, 10th order of calving (P < 0,05). Further I found out that calves in the 1st order of calving have significantly lower average weight from the 1st calving mothers at the age of 120 days than the calves in the 2nd, 5th, 7th, 10th order of calving. The average weight of calves at the age of 120 days is about 9,29 kg lower than weight of calves in the 2nd order of calving. On the other hand the average weight of calves at the age of 120 days in the 5th order of calving is about 18,37 kg higher than weight of calves in the 1st order of calving. Similarly, calves in the 7th order of calving have about 13,63 kg higher weight than calves from the 1st calving mothers. And also calves at the age of 120 days in the 10th order of calving have about 15,78 kg higher weight than calves from the 1st calving mothers.
In respect of influence of year of birth was identified statistically significant different level (P < 0,01) of the average birthweight in year 2013 compared to other years. Similarly there was demonstrated the statistically significant difference (P < 0,01) regarding to the average gain from birth in year 2013 compared to other years. The average birthweight in 2013 was 34,91 kg. The lowest average birthweight of 31,65 kg was observed in 2015. And for instance in 2012 the average birthweight was 32,48 kg which is still about 2,43 kg lower than in 2013. Regarding the evaluation of the average gain from birth till the age of 120 days there was only reported value of 1046,78 g in 2013. The best year regarding the average gain from birth till the age of 120 days was year 2014 which represented value of 1214,37 g.
In the observed breed there took place births predominantly from January till June. Regarding the month of birth there was demonstrated statistically significant difference (P < 0,05) in respect of average birthweight of calves born in May 31,98 kg compared calves born in February 32,55 kg and in March 33,07 kg. Further was identified statistically significant difference regarding the average gain from birth for calves born in June 995,28 g compared to calves born in February 1197,87 g (P < 0,01). Similarly in March was reported value of 1181,18 g (P < 0,05). The last statistically significant difference (P < 0,01) was identified in respect of average weight at the age of 210 days of calves born in May 199,55 kg and in June 201 kg in comparison to calves born in January 282,05 kg, in February 284,65 kg, in March 277,21 kg and in April 277,76 kg. To conclude, based on the statistical analysis, conclusive results and literary sources there had been confirmed hypothesis that internal factors positively affect the growth abilities of calves.
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Gas exchange characteristics in relation to genotypes in spinach (Spinacia oleracea) under water stress
Helebrantová, Aneta ; Hnilička, František (advisor) ; Pazderů, Kateřina (referee)
The bachelors dissertation was compiled on theme of: Gas exchange characteristics in relation to genotypes in spinach (Spinacia oleracea) under water stress.
Spinach (Spinacia oleracea) is, similarly as the others leaf vegetables, difficult crop in terms of providing the sufficient level of moisture, therefore the attention is drawn to the varieties of spinach which are resistant to the water stress. Thus the target of cultivation is to find plant which will be resistance to influence of the water stress.
In climabox of department of botanics and physiology was founded experiment with three species of spinach: Misano F1, Monores a Matador. The temperature mode was set to 21 °C during the day and 17 °C during the night. The light mode was set to 16 hours of light and 8 hours of dark per day. Maximum light level in climabox was 800 micromole. The plants were cultivated in 4 recurrences, diagram of experiment is involving two variants: control and stress. The plants in control variant were cultivated in substrate, which was irrigated during the whole time of experiment by 250 ml of water. For the plants in stress variant the supply of water was suspended for 10 days and the substrate was naturally continuously dehydrating. After 10 days the water supply was restored (rehydration) for plants in stress variant, same as level of control variant. The observation was made for the speed of gas exchange (photosynthesis and transpiration) in two-day interval. The speed of gas exchange was measured on leaf area with infrared gasometric gas analyzer Lpro+ (ADC Bioscientific, Hodeson, UK). Measured was conducted in morning hours according. Based on the measured values of photosynthesis and transpiration we calculated water usage effectiveness (WUE).
Based on obtained results is evident that the highest average speed of photosynthesis in control variant was observed at variety of Monores (12,10 micromole CO2.m-2.s-1) and lowest at variety Misano (11,58 micromole CO2.m-2.s-1). The highest average speed of photosynthesis in stress variant was measured at variety of Matador (9,43 micromole CO2.m-2.s-1) and lowest at variety Monores (8,76 micromole CO2.m-2.s-1). There was observed decrease of photosynthesis for each of variety during the water stress.
The highest average values of transpiration were observed at variety Monores (1,97 mmol H2O.m-2.s-1), lowest at variety Matador (1,68 mmol H2O.m-2.s-1). Stressed variety Misano reached level of photosynthesis 1,82 mmol H2O.m-2.s-1. Control variety Matador reached speed of transpiration 1,54 mmol H2O.m-2.s-1 and variety Monores 1,85 mmol H2O.m-2.s-1.Variety Misano was on same level of control variant as stress variant.
The most sensitively reacted variety Monores, which usage of water was 4,43 (10-3). Variety Matador managed the stress well, the usage of water was 5,60 (10-3).
Obtained results confirmed hypothesis of genotype differences depending on water deficit, thus there are differences between gas exchange and WUE in control and stress plants.
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A reaction of grassland to application of liquid fertilizer DAM 390
Netřebová, Lucie ; Hrevušová, Zuzana (advisor) ; Jiří, Jiří (referee)
This theses summarizes results obtained from studying of one-year experimental pot of permanent grassland which was established in Vítězná - Záboří in 2015. There has been observed 4 levels of nitrogen fertilization on the meadow plot (without fertilizing, 30 kg N.ha-1, 60 kg N.ha-1 and 90 survey was influence of nitrogen on the change in species richness by using weight share representation of individual agrobotanical groups in the stand (grasses, legumes, other forbs).
Results of this experiment were considerable affected by unfavourable weather events in the observed year. We should bear in mind the short time of this experiment. Positive unofficial effect of nitrogen on higher yield of above-ground biomass was recorded, especially by using 30 kg N.ha-1 and 60 kg N.ha-1. There is an exception at using 90 kg N.ha-1 with yield off balance. From the perspective of infuence of nitrogen on changes of species richness the increasing of doses of fertilizing has shown especially in increase proportion of grasses while present retreating herbs in the species composition of the reference stand. However results of experiment are not statistically different. We can not confirm studies which state decrease proportion of legumes in the grassland due to nitrogen fertilization from results of this experiment.
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The documentation of the educational trail "Dobrošov" fort (Czech Republic)
Kopecký, Tomáš ; Skalický, Milan (advisor) ; Jan, Jan (referee)
The educational trail Dobrošov fort is situated in the village of the same name. It is an advance specified marked route which leads in the footsteps of the border fortification from 1935 - 1938.
The length of the route is approximately 4 km and visitors are mainly informed about historically interesting places in the surrounding and about the events before World War II. Historical affairs are presented in the form of information panels at individual stops. The panels tell visitors not only about historical military facts of 20th century but also about the building of the fortress fortification Dobrošov.
The educational trail has eight official stops. On the route, visitors can see additional panels with the description of the light fortification and they learn interesting things about the surrounding.
The content of this thesis is a brief characteristic of events in Czechoslovakia before World War II. There is a description of the fortification system of a fort at that time in Europe and Czechoslovakia. Furthermore, this thesis deals with educational trails in general from the viewpoint of their functionality and equipment. This study points out legislation and illustrates new trends of this form of tourism.
The aim of this thesis was to document the educational trail Dobrošov fort, with the focus on historical events of fortification building in Czechoslovakia before World War II. The analysis of the trail and information panels was conducted. A questionnaire survey found out the needs of visitors and highlights the usefulness of this trail. The questionnaire survey had another important role for findings of stated hypotheses. Four scientific hypotheses were formulated. The first one is if the access to more objects increases, its attractiveness and attendance will also increase. The second hypothesis is whether the visitors are satisfied with the infrastructure of the educational trail. Furthermore, the awareness of the educational trail helps to its high attendance, and both citizens and tourists will be happy about a fortification extension which also includes the preservation of decaying objects in the surrounding. The used methods for achieving these aims were the observation and questionnaire survey in the location of the educational trail.
The conclusion shows that the access to more objects will increase the attractiveness and attendance of the trail. However, the visitors are not satisfied with the infrastructure of the trail. The awareness of the educational trail contributes to the high attendance. The hypothesis that local citizens and tourists will be happy about the trail extension which includes the preservation of decaying objects in the surrounding was disproved.
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Assess the current state of nutrition of horses in the specific stables and proposal optimal doses according to the their performance
Burcalová, Helena ; Mudřík, Zdeněk (advisor) ; Václav, Václav (referee)
This work deals with calculations of feed rations of selected thoroughbred horses at the High School of Horse Racing in Prague together with an assessment of economic aspects of horse feeding. The school is mainly focused on the education of jockeys, which means that the work with horses has to conform to the school requirements that need not meet the ideal feeding rhythm of the horses.
United Kingdom norms were compared to the selected Czech norms. Focus was mainly on the racing season, which provides direct comparison of the feed rations with the horses results in the races. The racing season has the highest energetic requirements that need to take into account not only the requirements for the races themselves but also for the resting periods. Feed rations were measured and fed in precise time intervals. Main feeds were oats, hay, bran and Spillers feeds.
During the assessment period a negative side - effect of high temperatures during consecutive heat - wave periods was observed. This aspect may prove important in the future and needs to be taken into account not only for feeding but for the entire horse training.
Out of five examined horses, exceedance of digestible energy requirements was observed with two horses and underestimation with one horse. For two of them the anomaly was in norm. However, the horse with the highest exceedance was experiencing difficulties with feeding, always leaving a part of the ration intact. This made it necessary to adapt the rations to a higher figure than calculated.
Economic aspect of horse feeding needs to be taken into account. The feeding expenses can be decreased by a suitable selection of a feeding mix, however the savings in this regard are not fundamental.
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Abundance of house sparrow (Passer domesticus) and other synantropic species in small settlements around Prague
Řezáčová, Andrea ; Šmejdová, Lucie (advisor) ; Zasadil, Petr (referee)
In the surroundings of Prague, as well as in many other parts of Europe the population numbers of synanthropic species were reduced in recent years. These changes in abundance are attributed to the change of use of agricultural landscapes, the loss of small poultry farms and mainly a change in the character of the construction. The aim of this thesis was to ascertain the abundance of selected species of synanthropic birds and compared their numbers with the data obtained in 2012. In this study were also evaluated differences in the abundance of these species in the localities of old construction area and new construction area. There were selected 3 main leaders, house sparrow (Passer domesticus), tree sparrow (Passer montanus) and eurasian collared dove (Streptopelia decaocto) from all monitored species for the statistical processing of the results. Census was carried out in a total of 40 squares, 20 of which was located in an old construction area and 20 in the construction of the new. All of the site lies in an area with a high degree of suburbanization in the central region an in around of Prague. The study
area covered an area of 200 x 200 meters and were visited a total of twice during the nesting period in the spring of 2015. The results show the preferences of the old buildings for the house sparrow and the eurasian collared dove. Tree sparrow was more numerous in the localities, characterizing the new housing development. For eurasion collared dove was observed increasing trend in abundance, while in both species of sparrows was recorded its decline. The most numerous species was the house sparrow with a total of 209 individuals, on the contrary, at least individuals, 23, was noted for european serin (Serinus serinus).
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Winter Density of House Sparrow in Different types of Rural Settlements along the Czech – Austria Border
Oberpfalzerová, Michaela ; Zasadil, Petr (advisor) ; Šmejdová, Lucie (referee)
From the point of view of the Czech Republic in the last 80 years we can specify two periods, which had a significant impact on the general character and development in agriculture. The first period can be traced to the 50s of the last century, in which the concentration of agricultural production headed towards to factory farming. The second one began at the beginning of the 90s. At that time a sharp downtown of animal production occurred and the sooner concentrated production began, after the regaining by previous owners, to erode into what it is today private agriculture.
This thesis aims tocontribute to a confirmation of rejection of the hypothesis that the above described changes have a negative impact on the selected avian species. These species are represented by significantsynanthropic birds: house sparrow (which is a crucial representative in this thesis), tree sparrow and collared dove. Thirty municipalities were chosen to emphasize the differences in quantity of these species, half of them located on each side of the border with the Czech Republic and Austria. The comparison of quantities of bird populations took place in the surroundings of the cities Znojmo and Retz.In this thesis the Republic of Austria acts as a country which has not been burdened by a substantial economic development and which has applied the continuous market economy since the end of the Second World War.
In the individual squares the numbers of the representatives of the studied species and the environmental characteristics were recorded during the winter period. The significant differences in the numbers of birds at selected locations were demonstrated using a multifactor ANOVA STATISTICA 12. Moreover, the impact of selected environmental variables on the abundance of observed species was also analysed. It was found that the selected species depend on an inclusion into the individual states as well as on a classification into various biotope types. Finally, the dependence on the area of trees and bushes, the proportion of new buildings and the presence of poultry in the square were also observed. The most numerous species in the Republic of Austria was the house sparrow, the most abundant species in the Czech Republic was the collared dove. A slight increase in the occurrence of the collared dove was recorded in both states. Tree sparrow as a species occurred in relatively large numbers, but only locally. It was found that the populationsof the above mentioned species were generally more numerous in the Austrian municipalities. An interesting fact is that most of the observed individuals in the Czech Republic occurred in village built-up areas rather than in agricultural premises, as it was initially estimated.
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