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Expression of AMPA glutamate receptor subunits in the suprachiasmatic nucleus of the rat
Červená, Kateřina ; Bendová, Zdeňka (advisor) ; Zemková, Hana (referee)
The main mammalian circadian pacemaker stored in suprachiasmatic nuclei of the hypothalamus (SCN) is adapted to changes in the external environement by synchronization of its endogenous period with periodic changes of light and dark during day and night. The information about light travels via glutamatergic retinohypothalamic tract to the ventrolateral part of the SCN. Activation of ionotropic glutamate receptors in this area provably mediates the transfer of information about light on the transcriptional mechanism of light-sensitive cells. The role of the NMDA type of ionotropic glutamate receptors is well studied in this field and it is known that some NMDA receptor subunits show a circadian rhythm and an increased expression after a light pulse. Signalization via AMPA type receptors is much less elucidated. The aim of this thesis was to determine which AMPA receptor subunits are expressed in the SCN of the rat and if these subunits show a daily rhythm of expression and a reactivity to light pulse, as well as to outline the possible roles of distinct AMPA receptor subunits in the SCN. Keywords: circadian rhythms, suprachiasmatic nuclei, glutamate receptors, AMPA
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Circadian clock during ontogenesis
Olejníková, Lucie ; Sumová, Alena (advisor) ; Hock, Miroslav (referee)
Circadian system enables adaptation of organisms to periodic changes in environment on the Earth. In mammals, it consists of central pacemaker in the suprachiasmatic nuclei (SCN) of hypothalamus and of oscillators that reside in other brain areas as well as in the peripheral organs and tissues. Ontogenetic development of the circadian system is a gradual process and the most dramatic changes undergo during the late embryonic and early postnatal stage. For its proper function, not only the morphological development of its individual parts, but also development of their entrainment to external environment and among each other, is important. The oscillations in clock gene expressions in the SCN occur already before birth, but in view of the fact that the levels of their protein products are undetectable, at this developmental stage, the ability of SCN to generate these oscillations in vivo has been discussed. After birth, the levels of these proteins rise and the rhythms in clock genes expression achieve the adult-like level at the postnatal age, when the synaptogenesis in the SCN is completed. The presence of a functional maternal circadian system is not necessary for the endogenous development of the SCN clock in pups, because the maternal SCN only entrains the clock and the circadian oscillations thus...
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Synchronization of circadian system during prenatal and early postnatal development
Houdek, Pavel ; Sumová, Alena (advisor) ; Novotná, Růžena (referee)
One of the few attributes common to almost all living organisms is an ability to generate and maintain endogenous rhythms, which are controlled by a biological clock. The processes, which recur with a period of about 24 hours, are known as the circadian rhythms. The circadian clock controls rhythms of molecular, physiological as well as behavioral processes and adapts their activity to regularly appearing changes in day and night or season. In case of mammals, central oscillator is located in the hypothalamic suprachiasmatic nuclei (SCN). The SCN clock entrains rhythms of peripheral oscillators located in cells of other tissues. The central oscillator itself is synchronized with external environment mainly by a light-dark cycle, however, other cues can entrain the SCN clock as well. For example, during prenatal development, entrainment of a fetal clock is entirely dependent on non-photic cues derived from maternal organism. This study aimed to investigate a mechanism of the communication between the maternal and fetal central oscillators. A hypothesis was tested whether maternal melatonin may play a role in entrainment of the circadian clock in the fetal SCN. Furthermore, a mechanism, how melatonin may entrain the fetal clock was investigated at molecular level. The results provided evidence, that...
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The role of glucocorticoids in circadian system
Tejkal, Karel ; Sumová, Alena (advisor) ; Forman, Martin (referee)
Glucocorticoids are mammalian steroid hormones secreted from the adrenal gland. The basal levels of glucocorticoids show a pronounced diurnal rhythm with maximum at the beginning of the active period and minimum at its end. Glucocorticoids have an influence over a variety of metabolic functions and their secretion is tightly regulated. This regulation also depends on the circadian system, which utilizes glucocorticoids to entrain the peripheral tissues by inducing rhythmic gene expression. The mechanisms by which glucocorticoids influence mammalian circadian system has not yet been precisely defined, especially concerning the influence of glucocorticoid signalling on gene expression in different tissues and the dynamics of glucocorticoid receptor (GR) occupancy. This thesis studies the influence of ablation of glucocorticoid signalization induced by adrenalectomy on the clock gene expression of in the central clock in the suprachiasmatic nucleus and peripheral clocks in the hippocampus and distal colon. The effect of adrenalectomy on gene expression is compared with the effect of restricting the feeding time, which has also been shown to affect glucocorticoid levels in the body. Other experiments were aimed at elucidating impact of changing the activity of GR on gene expression using synthetic GR...
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Phototherapy
Joklík, Vojtěch ; Škoda, Jan (referee) ; Štěpánek, Jaroslav (advisor)
This thesis describes relationship between human body and light. It is focused on perception of light with human eye, skin and their reaction to incident light and its intensity. Then it contains short description of Icterus Neonatorum (yellow-gum), Constitutional Dermatitis, Lepriasis and their treatment. It is followed by description of various sources of light, their use in medicine and reaction of body to them. This work includes measurements and evaluation of five sources of light.
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The Quality of Life Analysis with Regard to Circadian and Diurnal Rhythm of Pubescents and Adolescents in Children's Homes in the Region of Pardubice.
ŠTORKOVÁ, Tereza
This thesis deals with the quality of life of pubescent and adolescent in children's homes in the Pardubice region with regard to circadian and diurnal rhythms. In the theoretical part we define in detail the concept of quality of life, pubescence, and adolescence, biorhythms with a division between circadian and diurnal rhythms, sleep and institutional care. More attention is paid to sleep, its importance, quality and above all sleep cycles that are directly associated with the subsequent research area. In the research section, four research assumptions are made. Subsequently, we process data from eight orphanages acquired in the period from December 2011 to June 2012 in the Pardubice region. The questionnaire survey has 125 respondents of which 59 are teenagers in the age range 10-14 years and 66 adolescents between the age 15 and 18. For this purpose we used a shortened version of the standardized questionnaire for circadian typology CIT - Harada, Tailor (2010). The data was evaluated using basic statistical methods. It was found that on weekdays pubescents averagely go to bed at 09:15 pm and get up at 6:45 am. On weekdays, the average sleeping time is 9.5 hours. On the weekend pubescents averagely gone to sleep at 10:30 pm and get up at 8.30 am. The average weekend sleep is 10 hours. Adolescents go to sleep on average at 10:00 pm and get up at 6:45 am on weekdays. The average sleep on weekdays is 8 ? hours. On the weekend adolescents go to sleep on average at 11:15 pm and get up at 9:15 am. Which means that the weekend sleep is an average of 10 hours. Three assumptions were met, and that, the average sleep time for pubescents on weekdays is longer than the average duration of sleep in adolescents. Adolescents have a bigger problem getting up on weekdays before puberty. Pubescents evaluate their sleep rhythm from a diurnal perspective as sufficient. One assumption was refuted and that is, adolescents sleep on average less during the weekend than their pubescent counterparts. The research was useful as it brings together the issue of the quality of life of adolescents in educational institutions and can serve both for further research and as an indicator of the quality of life.
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Changes in body weight depending on circadian typology of adolescent girls during the year.
ROKOSOVÁ, Monika
This thesis deals with monitoring changes of body weight of adolescent girls at the age of 16 20 years old through one calendar year in the context of a season of the year and chronotypology of a personality. According to this typology the monitored people are divided into so called "larks", who are more active early in the morning and during the day and "owls" whose activity rises in the evening and at night. The theoretical part deals with circadian rhythms, their function, role in the life of an individual and also their disorders. It focuses on a developmental specification of adolescent girls, lifestyle in this period of life, changes of body weight and diet. Another part is the meaning of the sleep, its need and associated with good sleep habits and sleep hygiene. In the practical part there is described a methodology along with the results of the research and employment of this project in practice.
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Quality of life for children at primary school in Zliv.
BENEŠOVÁ, Aneta
In this diploma thesis deals with the issue of quality of life for children in primary school Zliv. The thesis is divided into two parts, theoretical and practical. The theoretical part focuses on characterizing the quality of life, a period of pubescence and the basic needs of children. In the practical part deals with research using questionnaires, which determines the value orientation and other areas of life-style, health and happiness. In the final stage, I present the results obtained, which are evaluated and graphically presented.
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Motion mode and circadian typology of village children in primary schools.(Research in Malenice,Strunkovice nad Blanicí,Vitějovice)
GLÜCKSELIGOVÁ, Lenka
This thesis examines the motion mode and circadian typology of village children under younger school age. In the theoretical part I have summarized findings on circadian rhythms, sleep, its disorders and sleep hygiene. Other chapters deal with physical activity and its relationship to health. Attention is also paid to the characteristics of the younger school age period and its development in the physical and psychosocial context. Research part brings the latest results of a questionnaire survey, to which I have used "Questionnaire of living rhythms and sleep regime 9 - 15 years" (Krejčí, Harada, 2010) and "Time shot of the day" (Mužík, Bártová, 2010). In the thesis there are compared two companies of the younger school age children - boys and girls from the three selected village schools. At the inconsiderable group of children I have found out disrupting of circadian hygiene with limited quality of sleep especially at weekend, irregular slightly and unbalanced food and increased intensity of playing computer and display games, particularly at boys. In the area of physical activity there were not found out worrying deficiencies.
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Analysis of the quality of life of preadolescents adolescents with regard to circadian and diurnal rhythms in children's homes in the South Moravian Region.
PEVNÁ, Radka
The thesis deals with analysis of the quality of life of preadolescents and adolescents with regard to circadian and diural rhythms in children's homes in the South Moravian Region. The first part characterises concepta of quality of life, pubescent, adolescent, circadian and diural rhythms. The work is focused on the temporal area of quality of life. It described the importance and quality of sleep, sleep cycles and sleep typology. The research deals with the questionnaire survey a total of 110 respondents (60 pubescent, 50 adolescents). The research method consists of a selection of questions from a standardized questionnaire circadian typology, CIT Harada, Krejčí, 2010. The data are analyzed using basic statistical methods
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