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The deposition of Ga and GaN nanostructures with metal core
Čalkovský, Martin ; Jarý,, Vítězslav (referee) ; Mach, Jindřich (advisor)
The presented thesis deals with preparation of GaN nanocrystals with a metal core. In the theoretical part of the thesis GaN with its properties and applications is introduced. Further, some of the preparation methods of GaN are presented, mainly focusing on MBE growth. Deposition of metal NPs from colloidal solution and the state of the art approaches to enhance luminescence of GaN based structures is discussed. The experimental part follows three steps of preparation of GaN crystals with the Ag core. In the first step the Ag NPs are deposited on the Si(111) substrate. In the second step the Ga deposition process is optimized and in the third step the deposited Ga is transformed into GaN. After the Ga deposition the samples were analyzed by SEM/EDX and SAM/AES. The properties of prepared GaN crystals with the Ag core were studied by XPS, photoluminescence and Raman spectroscopy.
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Fabrication and application of graphene-metal heterostructure in biosensing by Surface Enhenced Raman Spectroscopy
Hegrová, Veronika ; Édes, Zoltán (referee) ; Konečný, Martin (advisor)
This thesis deals with fabrication of graphene/metal heterostructures and their application in biosensing by surface-enhanced Raman spectroscopy. The heterostructures are fabricated by selective deposition of gold colloidal nanoparticles onto silicon substrate, where the termination of both the gold colloidal solution and the silicon surface is controlled. Further, the assembled gold colloids are overlaid by graphene monolayer. The experiments have proved, that it is possible to observe the presence of biomolecules at very low concentrations if the parameters of graphene/metal heterostructures are well selected. The combination of both graphene and metal SERS properties have demonstrated their appropriateness for utilization in bisensing.
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Optimization of surface-enhanced Raman scattering spectroscopy for study of biologically important biomolecules and their interactions
Šmídová, Natália ; Procházka, Marek (advisor) ; Vlčková, Blanka (referee) ; Michl, Martin (referee)
Title: Optimization of surface-enhanced Raman scattering spectroscopy for study of biologically important biomolecules and their interactions Author: Natália Šmídová Department: Institute of Physics of Charles University Supervisor of the doctoral thesis: Doc. RNDr. Marek Procházka, PhD. Abstract: The main goal of this thesis was to optimize surface-enhanced Raman scattering (SERS) spectroscopy for study of biologically important biomolecules. For that purpose we focused on substrates based on gold colloidal nanoparticles immobilized to silanized glass plates. Stable, uniform and highly reproducible SERS-active substrates have been prepared by using aminopropyltrimethoxysilane and citrate- reduced gold nanoparticles thermally stabilized after their immobilization. Model biomolecules 5,10,15,20-tetrakis(1-methyl-4-pyridyl)porphyrin (TMPyP) and 5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrin (TSPP) were studied on these substrates by using a classical Raman spectrometer in macro-mode and a confocal Raman microspectrometer. Conditions for SERS spectroscopy of porphyrins were optimized with respect to sensitivity and reproducibility. SERS microspectroscopy showed several advantages over SERS measurements in macromode: possibility of surface spectral mapping, easier manipulation with samples, shorter...
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Optimization of surface-enhanced Raman scattering spectroscopy for study of biologically important biomolecules and their interactions
Šmídová, Natália
Title: Optimization of surface-enhanced Raman scattering spectroscopy for study of biologically important biomolecules and their interactions Author: Natália Šmídová Department: Institute of Physics of Charles University Supervisor of the doctoral thesis: Doc. RNDr. Marek Procházka, PhD. Abstract: The main goal of this thesis was to optimize surface-enhanced Raman scattering (SERS) spectroscopy for study of biologically important biomolecules. For that purpose we focused on substrates based on gold colloidal nanoparticles immobilized to silanized glass plates. Stable, uniform and highly reproducible SERS-active substrates have been prepared by using aminopropyltrimethoxysilane and citrate- reduced gold nanoparticles thermally stabilized after their immobilization. Model biomolecules 5,10,15,20-tetrakis(1-methyl-4-pyridyl)porphyrin (TMPyP) and 5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrin (TSPP) were studied on these substrates by using a classical Raman spectrometer in macro-mode and a confocal Raman microspectrometer. Conditions for SERS spectroscopy of porphyrins were optimized with respect to sensitivity and reproducibility. SERS microspectroscopy showed several advantages over SERS measurements in macromode: possibility of surface spectral mapping, easier manipulation with samples, shorter...
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The deposition of Ga and GaN nanostructures on silicon and graphene substrate
Mareš, Petr ; Hospodková,, Alice (referee) ; Mach, Jindřich (advisor)
Presented thesis is focused on the study of properties of Ga and GaN nanostructures on graphene. In the theoretical part of the thesis a problematics of graphene and GaN fabrication is discussed with a focus on the relation of Ga and GaN to graphene. The experimental part of the thesis deals with the depositions of Ga on transferred CVD-graphene on SiO2. The samples are analyzed by various methods (XPS, AFM, SEM, Raman spectroscopy, EDX). The properties of Ga on graphene are discussed with a focus on the surface enhanced Raman scattering effect. Furthermore, a deposition of Ga on exfoliated graphene and on graphene on a copper foil is described. GaN is fabricated by nitridation of the Ga structures on graphene. This process is illustrated by the XPS measurements of a distinct Ga peak and the graphene valence band during the process of nitridation.
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Analysis of biological significant substances
Maděránková, Denisa ; Rychtárik, Milan (referee) ; Roleček, Jiří (advisor)
Selected methods of Raman spectroscopy, like surface-enhanced Raman spectroscopy and single molecule Raman spectroscopy, are described in this diploma work. The basis of two methods for numerical modelling of optical properties of micro- and nanoparticles are prefaced. The methods are Discrete Dipole Approximation and Finite Difference Time Domain. Micro- and nanoparticles are used in surface enhanced Raman spectroscopy and other nanospectroscopic methods. Further, the main instrumentation needed for Raman spectroscopy is described. The first part of experimental section of this work is numerical modelling of photonic nanojet that occures behind dielectric microparticles. This phenomenon leads to a new technique of confocal microscopy with Raman spectra measuring. The second experimental section contains results of Raman spectra measurement with beta-carotene and surface-enhanced Raman spectra of beta-carotene in silver-sol solution.
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