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Phylogeography of temperate plant species with the focus on Central Europe
Daneck, Hana
Phylogeography of temperate plant species with the focus on Central Europe Hana Daneck Charles University Prague Faculty of Science Department of Botany Supervisor: Prof. RNDr. Karol Marhold, CSc. Consultant: Mgr. Tomáš Fér, Ph.D. Praha 2012 Abstract This thesis presents contribution to clarification of postglacial history of temperate plant taxa in Europe with the focus on especially interesting region of Central Europe, for which diverse roles in postglacial plant histories were suggested. The first part of the thesis summarises general phylogeographical views and methodological approaches with the respect to species history after the last ice age in Europe. Further, the most important aspects of phylogeography of European temperate plant taxa are discussed. The second part contains a set of papers dealing with selected European temperate plant species, for which phylogeographical patterns throughout their present distribution area were inferred, including assumptions on the origin of their contemporary Central European populations and comparisons with another previously studied species.
Genetic and Morphological Variability of the European Genus Cochlodina (Mollusca: Gastropoda: Clausiliidae) with Focus on Species C. laminata (Montagu, 1803)
Szalontayová, Veronika ; Juřičková, Lucie (advisor) ; Drozd, Anna (referee)
This thesis focuses on the genetical and morphological diversity of plaited door snail (Cochlodina laminata). While small distribution ranges are typical for most species belonging to genus Cochlodina, the distribution range of C. laminata covers most of the European continent, except for its coolest and warmest parts. It has been previously suggested that this species might in fact be a complex of several species and large genetical as well as morphological diversity has been mentioned - however, yet undescribed - in previous studies. Sequences of two mitochondrial genes were used (16S rDNA, COI) and thirteen morphological characteritics have been assessed to investigate this diversity. I discovered that the current concept of C. laminata as a species is not in accordance with the discovered genetical nor morphological variability. The original species C. laminata/C. dubiosa form a common species complex and also interpretation of C. fimbriata will need to be assessed in more detail in the future. Other Central European species are valid species.
Phylogeography and genetic variation of benthic crustaceans in European inland waters
Pešek, Pavel ; Petrusek, Adam (advisor) ; Sacherová, Veronika (referee)
This thesis summarises published information about geographic distribution of genealogical lineages and genetic variability of benthic crustaceans of European continental waters, and evaluates the historical factors that affected their distribution. it is focused mainly on species for which multiple or in-depth studies have been published. A particular attention is given to crayfish of the genus Austropotamobius. Present distribution of genealogical lineages has been affected by changes in connectedness and availability of aquatic habitats. Species lineages often split in consequence to geological and climatic processes during the Tertiary, as exemplified on species complexes of Austropotamobius crayfish, species lineages of the amphipod Gammarus fossarum species complex, and the isopod Asellus aquaticus. The distribution of intraspecific lineages has been strongly affected by Quaternary cycles of glaciation, which not only destroyed many habitats by moving glaciers but also created new colonisation routes. Such routes were peri-glacial lakes, which had been used by Gammarus lacustris, or retreat of sea level, which enabled white-clawed crayfish to colonise England, and a freshwater crab to colonise Italy. Changes in sea levels are also the likely reasons for colonisation of continental waters by the...
Fylogeography and intraspecific variability of the cetoniid beetle Potosia Cuprea
Fuchsová, Aneta ; Šípek, Petr (advisor) ; Růžička, Jan (referee)
The presented thesis deals with the intraspecific variability of flower beetles species complex Potosia cuprea (Fabricius, 1795), which is a complex of taxa at the species and subspecies level. Flower beetles from species complex Potosia cuprea are among the most variable Palaearctic Cetoniinae at all. Taxa included in the complex produces chromatic range varieties which were, and still are, perceived differently by different authors. Taxa included in the analyses come primarily from western Palaearctic region, with the main emphasis on European species and subspecies. The aim of this work is the use of molecular genetics methods to verify the justification of their species or subspecies level. At all, there were obtained sequences for two mitochondrial genes (cytochrome b and cytochrome oxidase I) from 14 taxa species complex Potosia cuprea and three related species P. angustata (Germar, 1817), P. fieberi (Kraatz, 1931) and P. marginicollis (Ballion, 1870). Molecular analyzes based on COI dataset and concatenate of CytB and COI revealed the existence of a "European" clade P. cuprea, which includes subspecies: Potosia cuprea bourgini (Ruter, 1967), P. c. brancoi (Barraud, 1992), P. c. cuprea (Fabricius, 1775), P. c. metallica (Herbst, 1790) a P. c. obscura (Mikšić, 1954), to which also fit two...
Speciation of the genus Lethrus (Coleoptera: Geotrupidae) of the eastern mediterranean
Drožová, Dana ; Janšta, Petr (advisor) ; Bezděk, Aleš (referee)
Earth-boring dung beetles of the genus Lethrus Scopoli, 1777 belong to the family Geotrupidae, classified into three subfamilies (Geotrupinae, Lethrinae a Taurocerastinae). The description of about 120 species, divided into nine subgenera, is based on morphology and geographic distribution. Shape of mandibles, ventral mandible processes, pronotum and structure of external male genitalia are used as diagnostic morphological characters for taxa. The richest species diversity is known from Central Asia. The distribution area reaches, in the east, up to Mongolia and China, and in the west, up to Southeast Europe. All species are robust, relatively large flightless beetles with low dispersial ability. They feed on parts of fresh plants instead of feces like other dung beetles. Master thesis is focused on the species distributed in the Eastern Mediterranean and Central Europe. This area is inhabited only by nominotypical subgenus Lethrus. The main goal of thesis is to clear up phylogenetic relationships between species of subgenus and speciation events using molecular genetic methods. We have examined 91 samples of the genus Lethrus using two mitochondrial genes - cytochrome b (382 bp, 80 sequences), cytochrome oxidase I (815 bp, 87 sequences) and nuclear gene 28S rDNA (D2-D5) (1100 bp, 11 sequences)....
Phylogeography of Rousettus aegyptiacus in the Mediterranean region
Dundarova, Cheliana ; Hulva, Pavel (advisor) ; Janko, Karel (referee)
The genus Rousettus has distributional pattern unique among fruitbats comprising both Asia and Africa and reaching northern distributional limits of the family in Persia, Arabia and Mediterranean basin. This could be ascribed to the ability of echolocation, consequent cave dwelling, and presumably other site-specific adaptations, which enabled dispersal independent of forest block and surviving in Mediterranean type of climate. Using fastly evolving mitochondrial marker, we aimed to assess genetic variability, its geographic distribution and demography of northern populations of the Egyptian fruitbat (Rousettus aegyptiacus). Mitochondrial network indicates deep genetic divergence between disjunct Mediterranean and eastern African parts of the range. Basal position of Sinaic and Jordanian haplotypes within northern clade indicate important role of these regions in colonization of eastern Mediterranean. Generally, the northern haplogroup is moderately diversified with partial geographic localization of particular haplotypes. Significant isolation by distance pattern suggests relatively pronounced site fidelity of particular colonies, at least in terms of maternal gene flow. Landscape genetics analyses indicate discontinuities in distribution of mitochondrial genetic variability, in some cases correlating with...
Phylogeography of temperate plant species with the focus on Central Europe
Daneck, Hana ; Marhold, Karol (advisor) ; Štech, Milan (referee) ; Tremetsberger, Karin (referee)
Phylogeography of temperate plant species with the focus on Central Europe Ph.D. Thesis Hana Daneck Charles University Prague Faculty of Science Department of Botany Supervisor: Prof. RNDr. Karol Marhold, CSc. Consultant: Mgr. Tomáš Fér, Ph.D. Praha 2012 2 Summary This thesis presents contribution to clarification of postglacial history of temperate plant taxa in Europe with the focus on especially interesting region of Central Europe, for which diverse roles in postglacial plant histories were suggested. The first part of the thesis summarises general phylogeographical views and methodological approaches with the respect to species history after the last ice age in Europe. Further, the most important aspects of phylogeography of European temperate plant taxa are discussed. The second part contains a set of papers dealing with selected European temperate plant species, for which phylogeographical patterns throughout their present distribution area were inferred, including assumptions on the origin of their contemporary Central European populations and comparisons with another previously studied species. Paper 1: Phylogeographic pattern of the European forest grass species Hordelymus europaeus: cpDNA evidence. This paper presents phylogeographical pattern based on chloroplast haplotype variation covering the...
Phylogeography of the genus Squalius in Albania
Lerch, Zdeněk ; Švátora, Miroslav (advisor) ; Kalous, Lukáš (referee)
The thesis is focused on the issue of the Squalius genus phylogeography in Albania in the Balkan region. Phylogenetic analyses of sequence variation at mitochondrial genes (cytochrome b) were used to examine these issues for the freshwater fish genus Squalius from various river systems in the Adriatic Sea region. There were identified 3 genetic lineages of vague taxonomic position where the genetic variation amount to 1.6-2.1 %. The first lineage is distributed in north of Albania and in the whole Periadriatic region, the second lineage is especially spread to northern part of Albania and the third lineage covers especially the zone of the European ancient lake systems on the Balkan Peninsula (Lake Ohrid and Prespa), from where expands to the southern part of Albania. There sympatrically coincide the both lineage in the hydrological river-lake system of Drin and Skadar. The phylogenetic and taxonomic position of the Squalius genus in the region seems to be interesting topic for subsequent and more detailed study. Keywords: Squalius, Albania, cytochrome b, phylogeography
Colonisation of Central Europe by benthic frehwater crustacean Asellus aquaticus (Isopoda, Crustacea)
Střížek, Antonín ; Sacherová, Veronika (advisor) ; Špaček, Jan (referee)
Longterm climate changes are an inseparable part of the evolution of Earth. In the last few milions of years the changing of glacials and interglacials was as ordinary and regular phenomenon as changing from day to night or from spring to summer. These cycles also have similar influance on evolution of nature on Earth. Eventhough the state of nature appears to us stable for the few last human generations, the reality from the long term point of view is differnt. During these cycles, the location of climatic zones, size of glaciers, deserts, savannahs, steppes or rainforests have changed. Organisms changed locations of their areas of distribution, many nowadays widespread species were pushed into isolated local populations. This Master's thesis reveals the impact of glacial cycles on a freshwater crustacean aquatic Isopod (Asellus aquaticus). Very variable mitochondrial COI gene was sequenced within 139 individuals of this species from 62 different localities in Europe. This data were included into an extensive scope of an already known phylogeographic structure of the continent. An Aquatic Sowbug shows a quite high rate of a genetic heterogenity (maximum Nucleotide Divergence discovered is 0,132 and average is 0,016) in the area of the Czech Republic. Where there are found representatives of two...
Diversity, distribution and genetic structure of the tribe Pipistrellini
Eliášová, Kristýna ; Hulva, Pavel (advisor) ; Fornůsková, Alena (referee)
This literature review discusses phylogenetic relationships within the family Vespertilionidae with a detailed focus on tribus Pipistrellini relations and then the individual genera and species within this tribe. A historical summary of efforts to resolve the phylogenetic relationships within the taxon first on the basis of morphological studies, comparison of karyotype and most recently on the basis of genetic markers. Mitochondrial genetic markers are useful in studying phylogeography, as this paper shows on the example of the Pipistrellus species complex and on species Nyctalus azoreum, Nyctalus leisleri, Nyctalus noctula and Pipistrellus abramus. Furthermore, this work describes the current distribution of individual taxa of tribe, the fossil record and migration behavior of European temperate species in the context of their population structure (also studied in some species using mtDNA and microsatellites) and Annex shows the areas extending all five genera Pipistrellini tribe. Key words: Pipistrellini, phylogenenetics, phylogeography, distribution, population structure

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