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Splicing of atypical introns in S. cerevisiae
Cit, Zdeněk ; Půta, František (advisor) ; Pichová, Alena (referee)
Pre-mRNA splicing is a vital process of gene expression important for all eukaryotic organisms. For the proper function of this very complex and dynamic event the presence of few specialized RNA and many proteins that hold a variety of tasks is necessary, not only inside the splicing complex itself, but also beyond this complex. The Prp45 is one of the proteins involved in pre-mRNA splicing in yeast Saccharomyces cerevisiae. Its human homologue, SNW1/SKIP, is involved in splicing but also in other crucial cell processes. The Prp45 protein was reliably reported only to participate in the second transesterification reaction of splicing. But there are also data suggesting its possible involvement in the first transesterification reaction. This work provides further evidences linking protein Prp45 with the first splicing reaction, obtained by the research of cells carrying the mutant allele prp45(1-169). Cells carrying this allele show dropped splicing and accumulation of pre-mRNAs. This thesis therefore also investigated the possible influence of Prp45 protein on the RNA export from the nucleus to the cytoplasm. But no connection between this protein and RNA transport was discovered. Keywords pre-mRNA splicing; Saccharomyces cerevisiae; Prp45; Mer1; Mud2; Prp22; Rrp6; AMA1; SNW1/SKIP
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European lace of renaissance and baroque
Svobodová, Veronika ; Jarošová, Helena (referee) ; Kybalová, Jana (advisor)
Lace is a decorative ajour textile. Real laces can be divided into needle laces and bobbin laces. There are two types of bobbin laces: straight and part-lace. These both techniques were developed in the epoch of renaissance, in the first half of the 16th century. The needle lace was developed from a cutwork. The immediate precursors of the needle lace were "point coupe" and "reticella" techniques. The bobbin lace was developed from a passementerie. The bobbin laces as well as the needle laces appeared probably at once in Italy and Flanders. The first laces have a geometric decor. Linear or plant decors were used in the first half of the 17th century. Fine and light laces with a flower motif rise in the 18th century. In the end of the 18th century, the motifs are very small and the most of the laces fill mesh ground. In the epoch of the renaissance and the baroque, good conditions for complex figurative scenes were allowed. A grateful space for (riche -great) religion scenes was given. The lay scenes, almost genre, symbolic and hunting scenes can be seen on the lace, too. All the 16th century and in the first half of the 17th century appeared a lot of Pattern Books first for needle laces and then for bobbin laces in Germany and Italy. It is a proof (evidence) of a great vogue of the lace.
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The Book of Job. How to deal with suffering?
Millerová, Pavlína ; Heryán, Ladislav (advisor) ; Širka, Zdenko (referee)
Questions concerning evil and suffering are those, which people ask the most often. What is their reason and purpose. The authoress seeks the answer with the aid of the book Job from the Old Testament. At the beginning she acquaints us with the book and analyzes its individual parts. She takes notice to the reflection of the story of Job in theological literature as well as in fine arts. The book of Job depicts with evil and suffering. Authoress therefore afterwards thinks of these terms in a wider connection. According to her, no simple answer likely exists. Instead, the final part of the work brings in burning questions, concerning past and present. It calls appeal for our further thinking about them. Powered by TCPDF (www.tcpdf.org)
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Multilokusová charakteristika symbiontů entomopatogenních hlístovek rodu \kur{Steinernema}
FAKTOROVÁ, Lucie
During the evolution some groups of organisms have become coevolutionary associated with other groups, as is the case of host symbiont systems. To explore coevolutionary history of hosts and their associated symbionts, phylogenetic reconstruction of symbionts and phylogenetic reconstruction of hosts are usually compared. Coevolution is described by coevolutionary events (cospeciation, host switch, duplication, failure to diverge events and linage sorting events). The aim of this work was to test the suitability of MLST method for the complex of entomopathogenic nematodes from the genus Steinernema (with detailed analysis of Steinernema feltiae) and their symbiotic bacteria Xenorhabdus bovienii and subsequently use cophylogenetic comparative analysis to determine their level of cospeciation.
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"And because the previous retired peasant is still alive ... ." The issue of rent-charge on the Třeboň domain in the 18th century
PECKOVÁ, Štěpánka
The presented thesis originated thanks to the support of the project of Grantová agentura Jihočeské univerzity Nr. 130/2010/H (The society of Czech regions in 17th up to 20th century. The area of economical, social, cultural and religious structure of Czech regions and their transformations during the 17th-20th century). The presented thesis is dealing with, on the example of Třeboň domain, the period transformation of the institute of the rent-charge and the position of retired peasants during the 18th century on the Třeboň domain, specifically in years 1725-1735 and 1775-1785. For the research there were used two from four books of rent-charge?s contracts deposited in the Státní oblastní archiv (public regional archive) in Třeboň. There was chosen the micro historical method of the research as the basic methodological starting point for the analysis of particular cases. Also the exercise of certain lifelong strategies in the family environment in the country has become the subject of the explorational interest. The goal of this thesis was to ascertain, which circumstances influenced the origin of the rent-charge and why it was required. The analysis of chosen lifelong advancements of individual groups leaving for the retirement revealed, that in the person?s behavior in the rent-charge were reflected rooted models of behavior and action, but there was also possible to grasp even the individual strategies. It was possible to draw this conclusion even in the case of the investigation of the content aspect of the rent-charge. The research points out the difference of opportunities, which presented themselves to retired peasants and sustains, that the rent-charge did not mean the end of life, but its partial stage.
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The Velvet Revolution's influence on public life in Milevsko during 1989-1990 era
Wimmerová, Lucie ; Dvořáková, Vladimíra (advisor) ; Petřík, Martin (referee)
The thesis describes the political events initiated on November 17, 1989, which, after 42 years, led to the end of one-party rule and started the long process of democratization in Czechoslovakia. It analyzes the similarities and differences between the Milevsko policy and the national policy from November 17, 1989 to the June 1990 elections to the Federal Assembly and the Czech National Council. It describes how the democratization process, started with the Velvet Revolution in Prague, reflected in the public life of a small town. It answers questions like whether the political, economic and social changes in Milevsko were the initiative of its citizens, or those recommended by the revolutionary center in Prague? What was the attitude of Milevsko citizens towards these changes? Did they request replacement of politicians and municipal institutions as in Prague, or did they have specific requirements distinct from Prague?
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"Escape to the woods and for adventure" and his representation in Czech literature from the first half of 20. Century
NĚMCOVÁ, Věra
Bachleor work focuses on adventure stories in the Czech environment in the first half of the 20th century, especially on literature concerning the origin and expansion of tramp movement in our country, which is as a phenomenon. "Escape to the woods and for adventure" reflect in social life and literature influenced by running events. The student will follow different representations of this phenomenon especially in the literature type of "Rodokaps". It is also focused on the work of the German author Robert Kraft. This section includes a brief biography of the author, his work, and characteristics of the nine major novels that are connected by character hero Nobody.
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Prague Faculties of Medicine and Their Clinics in 1938-1945
Svobodný, Petr ; Hlaváčková, Ludmila (advisor) ; Hach, Petr (referee) ; Míšková, Alena (referee)
The developments that took place in Prague in 1939-1945 offer a particularly interesting starting point for analysing the impact which changing political circumstances can have on universities. The progress of events at Prague universities and institutions of higher learning at key points of the period of 1938/39-1945 does not readily lend itself to any sort of 'straightforward' characterisation that would take us from a Nazification of the German (and Austrian) universities after 1933 to their eventual de-Nazification after 1945, or from a closing of universities of the occupied country to their eventual re-opening after 1945 (as was, for example, the case in Poland). The parallel existence of both a Czech and a German university (and technical university), their complementary and at times strongly diverging histories at key moments of the 20th century, supply a rich source of material for a study of the complex interplay between politics, university education, and science. Present intention of this dissertation, however, is not to present these universities as a whole. It focuses just on their faculties of medicine, including their clinical background in Prague hospitals. Faculties of medicine have a unique position not only with respect to other parts of a university, but also in other areas of...
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