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Palliative care is provided by patient's family at home
KUČEROVÁ, Tereza
The thesis, titled Palliative Care Provided by Patient's Family in Home Environment, discusses the specific care needs of a terminally ill patient in his/her own home. Caring for an ill person in a home environment is beginning to be a growing trend in home care, but also it is an increasingly common wish of the patients themselves. The family is therefore required to have enough theory and practical knowledge in order to deliver a highly demanding personal care for their close family members. The aim of the thesis is to summarize the current situation of delivering palliative care in home environment in Czech Republic whilst concentrating on the specific needs of the patient and the needs of their family. The objectives of the research were to determine whether family members were introduces to the specifics of palliative care of terminally ill patients; to map their skills in delivering palliative care in home environment and to reveal the most common issues that family members come across in that home environment. The final objective was to map out services in regions of Central Bohemia and Liberec that are accessible to family members whilst caring for a terminally ill patient. The research part of the thesis was completed employing a method of qualitative investigation using semi-structured interviews with patient's family members and nurses providing hospice care in home environment. It emerged from the analytical interviews that the family's knowledge level regarding support for palliative care in home environment is very limited and in hospitals families are only given minimal or no support or information. As a final document for the thesis an informative educational brochure was created based on the results of aforementioned research, primarily targeting patient's family; its core objective is to improve information and collate the specifics of care that would allow the patient a dignified end to their life.
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How to prevent the proliferation of Legionella pneumophylis in hot water in the hospital Czech Krumlov
TUREK, Jan
This diploma thesis deals with microbiological quality of hot water in a hospital in Cesky Krumlov as well as hot water piping condition with respect to Legionella. It also deals with arrangements for improving the quality of hot water in this hospital. The subject and target of this thesis are the findings of the mentioned parameters based on own observations, technical documentation and analysis of warm water performed by an accredited laboratory. The hospital in Cesky Krumlov, the construction of which began in 1909, was built in 1911 as the 'Nemocnice Císaře Františka Josefa I.' As the time went by, new buildings were built up. The hospital was renamed to 'Všeobecná veřejná nemocnice' in 1942. In the following years the hospital expanded until the current condition. Water quality and presence of Legionella is a world-wide issue, which we must fight against. The thesis is divided into two parts. First, theoretical part, which includes both general and specific knowledge about Legionella, its health risks, the environment in which it occurs, possibilities of eliminating, suitability of the material for hot water pipes, gained in professional literature and a part of the Czech legislation dealing with public health protection, the law 'No. 258/2004 Coll.' in actual version and quality of drinking water and hot water. In the second, empirical part, I focus on the situation of the hospital in Cesky Krumlov from 2009 till 2015, concerning newly built boiler room and the whole distribution system of hot water regarding Legionella. Particularly I focused on materials used for ecologisation of the gas boiler room and materials used for hot water pipes and actual state of water distribution in individual buildings of this health care facility. I mentioned the analysis of the water samples performed by an accredited laboratory with corresponding technical documentation, which concerns the water distribution system, provided by the medical facility. I used secondary data analysis to evaluate these documents. Regarding the results from 2009 to 2015 the microbiological quality of warm water doesn't meet the limits defined in the Attachment No. 3 of the above mentioned law Order 252/2004 Coll. Limit value for colony forming units of Legionella is set to maximum of 100 CFU. In most of the samples this limit was exceeded by series of units up to hundred thousands of units. The last results in 2015 prove improvement of the unfavorable situation. In that year the analysis was tested three times. First in February, where the CFU units were 'only' in hundreds, with the top value of 550 CFU in the Postacute Care ward. Second analysis was performed in June that same year. Results were acceptable. The highest value was 64 CFU. Last analysis in the hospital was performed in December. Increased concentration of Legionella was again in the Surgery building. There were established of 1400 colony forming units. There were 300 CFU in the Postacute Care ward and the limit value of 100 CFU was in the ward of Internal medicine. The answers to research questions 'How to prevent reproduction of Legionella peumophylis in the water distribution system of the hospital in Cesky Krumlov?' and 'How to ensure a sufficient amount of quality hot water in the medical facility?' are included at the end of the stated recommendations. A risk factor causing the fast reproduction of Legionella is the old water distribution system in most of the buildings and low water temperature of water leaving the boiler, which is 55°C. All mentioned results and evaluations are included in this thesis as well as attached pictures, which show the environment and situation within the Health Care Institutions.
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The effect of pyrethroid based pesticides on fish
RICHTEROVÁ, Zuzana
Pyrethroids are ones of the most used pesticides worldwides. The widespread use and high stability of pyrethroids lead to the assumption of that their occurrence in the environment could be quite frequent. They can reach water ecosystem as pollutants. Residues of pyrethroids are not only detected in the water column, but also in sediments and in fish tissues. The first study was devoted to the product Nexide containing 60 g.l-1 of active substance gamma-cyhalothrin. Tested Nexide concentrations were 5, 25, 50, 100, and 250 µg.l-1. Early life stage test was used.Common carp (Cyprinus carpio) was tested. There were significant mortalities in all concentrations except the lowest concentration during the trial. The lowest concentration tested 5 µg.l-1 only caused a slightly increased mortality. This lowest concentration influenced the growth in length and weight negatively, decelerated ontogenetic development, and made the body surface of the individuals darker. Histopathology of individuals from this concentration revealed dystrophy in liver. Examination of kidney, intestine and gills did not show significant histopathological differences compared with control. The evaluation of selected parameters of oxidative stress demonstrated a significantly higher activity of detoxification enzyme glutathione-S.transferase (GST) and a significantly lower activity of defensive enzyme glutathione peroxidase (GPx) compared with the control group. The other examined parameters of oxidative stress such as catalase (CAT), glutation reductase (GR), and lipid peroxidation determined by using the thiobarbituric acid-reactive substances (TBARs) were comparable to the control group. Changes in oxidative stress parameters suggest that exposure of the organism to the product Nexide in the given concentration leads to dysbalance of defensive enzymes. The second study was devoted to the product Cyperkill 25 EC containing 250 g.l-1. Tested Cyperkill 25 EC concentrations were 7.2, 36, 72, 144, and 360 µg.l-1. The procedure of the trial was the same as the preceded one. There were 100% mortalities in all concentrations except the lowest concentration during the trial. The lowest tested concentration 7.2 µg.l-1 allowed 90% of individuals to stay alive till the end of experiment. The lowest concentration influenced the growth in length and weight negatively and decelerated ontogenetic development compared with the control. Any individual exposed to this concentration did not reach juvenile stage until the end of the trial. Dark pigmentation was visible in 68% of these exposed individuals on the last day. Similar darkening was visible in individuals from higher concentrations shortly before death too. Histological examination did not revealed significant changes in intestine, liver, kidney, and gills compared with the control group. Evaluation of selected parameters of oxidative stress demonstrated significantly lower activities of GST, GR, and GPx. Activities of CAT and TBARS were comparable with the control group. Changes in oxidative stress parameters suggest that exposure of the organism to the product Cyperkill 25 EC in the given concentration could induce oxidative stress and interfere with the activities of antioxidant enzymes. The presented thesis summarises actual data about pyrethroids and their influence on fish. The demonstrated effects confirm high susceptibility of early developmental stages of fish to tested pesticides. When interpreting the results, we have to take into account the fact that studies showed this risk even on single pyrethroid substances. But water organisms are exposed to many other more or less toxic products and substances in a real environment. These xenobiotics could react with each other and their mixture could even potentiate negative effects. The performed studies also clearly show the significant differences in the sensitivity of embryonic and embryolarval tests.
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Iniciace pohybu byčíku, signalizace a regulace pohyblivosti spermií ryb: fyzikální a biochemické řízení
PROKOPCHUK, Galina
The current study attempted to shed light on the regulatory processes and response arrangements of fish spermatozoa during the course of maturation and motility initiation. The first intent of this study was to improve the understanding of the mechanism underlying the acquisition of potential for sperm motility in sturgeon. Up to present work, the physiological process underlying sperm maturation in this species has not been described at all. Our results showed that sperm maturation in sturgeon occurs outside the testes because of dilution of sperm by urine and involves the participation of high molecular weight substances as well as calcium ions present in seminal fluid and/or urine. The second aim of the present study was to investigate the coping mechanisms in fish spermatozoa with osmotic and ionic activating mode, as well as in spermatozoa of euryhaline fishes, to various environmental conditions. We showed that alteration of environmental osmolality might affect the fish sperm in different ways, depending on fish species and modes of spermatozoa motility activation either osmotic or ionic mode. In response to osmotic stress caused by hypotonicity, carp spermatozoa regulated the flow of water across their cell membrane and increased their cytoplasmic volume during their short motility period. In contrast, no indications of sperm volume changes were observed neither in sterlet nor in brook trout spermatozoa, both of which having an ionic mode of motility activation. We also examined the mechanism by which sperm motility triggering in euryhaline fishes can adapt to a broad range of environmental salinity. Our results demonstrated that spermatozoa of euryhaline tilapia, Sarotherodon melanotheron heudelotii, reared in fresh-, sea- or hypersaline water can be activated in hypotonic, isotonic or hypertonic conditions of swimming milieu, provided Ca2+ ions are present at various levels. It was established that the higher the fish rearing salinity or the more hypertonic ambient media at spermatozoa activation, the higher extracellular concentration of Ca2+ ions is required. The results obtained in the present study allow suggesting that osmolality is not the main factor inhibiting sperm motility inside the testis in the S. melanotheron heudelotii. A third aim of this study was investigation of the regulation of motility initiation process and description of flagellar beating initiation in chondrostean spermatozoa. We detected that K+ inhibition of sperm motility in sturgeon can be by-passed due to the pre-exposure of sperm cells to a high osmolality shock prior to its transfer to K+-rich swimming media. Thus, we hypothesized that sturgeon spermatozoa may be activated by use of an unexpected signaling pathway, independent from regular ionic stimulation. The successive activation steps in sturgeon spermatozoa were investigated by high-speed video microscopy, using specific experimental situation, where sperm motility initiation was delayed in time up to several seconds. At motility initiation, the first couple of bends formed at the basal region begins to propagate towards the flagellar tip, but gradually fades when reaching the mid-flagellum. This behavior repeats several times until a stage where the amplitudes of bends gradually reach similar value, what eventually leads to sperm progressive displacement. The total period needed for the flagellum to switch from immobility with rigid shape to full activity with regular propagating bends ranges from 0.4 to 1.2 seconds. In conclusion, the results of the current study bring valuable pieces of information into the general understanding of the processes of maturation of fish spermatozoa, their adaptability to different physical and biochemical circumstances, the extra- and intra-cellular signaling as well as the regulatory mechanisms of motility activation in fish spermatozoa.
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Comparison of HPLC a GC-MS method for pesticide determination in soil
Chalbia Václavíková, Alena ; Kočárek, Martin (advisor) ; Havlík, Jaroslav (referee)
Physical and chemical properties of pesticides may vary significantly. Pesticides can be acidic, neutral or alkaline. Some compounds containing phosphorus, other sulfur, nitrogen or halogens. These atoms can be important for the detection of pesticides. Some of the compounds are volatile, others on the contrary not evaporate, which is also one of the important properties for chromatography. The diversity of pesticides actually makes it impossible to create a universal method, which is very impractical in terms of quality and time determination. Because that pesticides are one of the most chemicals in most countries. In the food and drinking water are limits on pesticides in order to avoid negative impact on public health. For residues in soil or surface water limits are set. (Lutz et al., 2006)
The objective of this thesis is to evaluate the possibility of using two types of chromatographs with different detectors for the analysis of pesticide. Pendimethalin which was chosen for this work, will be analyzed usinga liquid chromatograph with UV detektor and gas chromatography with mass detector. Both this method will be compared as well two extraction methods (Soxhlet extraction and shaking), and two extraction agents (methanol and acetonitrile).
Another point of the work is to provethe hypothesis that, for the determination of pendimethalin in soil is by gas chromatography using a mass spectrometer in comparison with the high performance liquid chromatography time and more expensive, but will achieve a higher yield and a lower detection limit of the pesticide.
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The Public Procurement for the Supplies
Holzäpfelová, Vlasta ; Civínová, Denisa (advisor)
The thesis deals with the theme of procurement of supplies in the Czech Republic from its beginnings to the present in the context of legal and economic developments. The current situation is analyzed on the basis of the Public Procurement Act, in connection with monitoring the preparation and adoption of new legislation. Legal issues will be extended by an overview of relevant laws and evaluate the problems in the formation of tender documents for the conclusion of framework agreements for the supply and reviewing compliance with the principles of the Act on financial control in public administration. Theme in the application of profiling on public procurement, focusing on a framework agreement designed to capture recurring delivery. The study is supplemented with practical experience in the field of public procurement for the conclusion of framework agreements, provides an analysis of the positive and negative aspects of this method of procurement, including proposed solutions. The practical part is elaborated on the basis of a comparison of the specific tender documents with the statutory legislation, qualitative research and secondary data analysis of tender documents for contracts to supply a valid framework contracts signed by the selected organization in the years 2012-2016.
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The introduction of quality management system according to ISO 9001
Kunzová, Kateřina ; Štůsek, Jaromír (advisor)
The thesis describes the design process and the documents required to meet the standards associated with the effective implementation of a quality management system according to DIN EN ISO 9001 in organization Zinková slévárna - Ing. Karel Dítě.
In the theoretical part of the work I explain the basic concepts and issues relating to the use of literature associated with the ISO 9001 Quality Management System. This section also describes the general process for implementing the standards within an organization. Following on from this, I have used the knowledge gained from the theoretical section within the practical part of the thesis.
The practical part of my work includes a description of the selected organization from several perspectives - it is a focus, organizational structure, customer and supplier portfolio. This section also analyses the current state of the organization and includes a proposal to meet the ISO standards. The final section contains the documents necessary for the implementation of ISO 9001 within the organization, so that the quality management system corresponds to the prescribed standards.
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The introduction of quality management system according to ISO 9001
Kunzová, Kateřina ; Štůsek, Jaromír (advisor)
The thesis describes the design process and the documents required to meet the standards associated with the effective implementation of a quality management system according to DIN EN ISO 9001 in organization Zinková slévárna - Ing. Karel Dítě.
The theoretical part of the work focuses on the history, where I explain the basic concepts and issues relating to the use of literature associated with the ISO 9001 Quality Management System. This section also describes the general process for implementing the standards within an organization. Following on from this, I have used the knowledge gained from the theoretical section within the practical part of the thesis.
The practical part of my work includes a description of the selected organization from several perspectives - it is a focus, organizational structure, customer and supplier portfolio. This section also analyses the current state of the organization and includes a proposal to meet the ISO standards. The final section contains the documents necessary for the implementation of ISO 9001 within the organization, so that the quality management system corresponds to the prescribed standards.
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Individual production of caravan
Kollar, Jan ; Lachnit, František (advisor) ; Kotek, Martin (referee)
The topic of this thesis is individual construction of the O1 category caravan on
mass produced cargo trailer chasiss. For simplifying administrative processes, the whole
construction is done as a rebuild. Thesis is about workflow of construction, legislative
requirements and documents that need to be present to all neccesary autorities. In thesis the
whole idea of building a caravan is covered. It starts from documentation, then buing of mass
produced chasiss is described, the build itself is described and in the end there is information
about registering of completed caravan according to czech law 56/2001 Sb. - o podmínkách
provozu vozidel na pozemních komunikacích (law about requirements for road driven
vehicles). In the thesis the choice of ideal mass produced chasiss is described, materials used
for construction are defined and necessary homologated parts needed for legal registration are
labeled.
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Use of Festuca arundinacea for decorative lawns
Doskočil, Jan ; Svobodová, Miluše (advisor) ; Hrevušová, Zuzana (referee)
Use of Festuca arundinacea for decorative lawns
Summary
Festuca arundinacea is briefly bentgrass with broader and coarser leaves. Hardly perennial grass, native to Europe, is well known for its high resistance of dry weather, low intensity of maintenance and resistance of high use. These qualities gains thanks to its well-developed and deep root system, which is capable to gain water and nutrients from deeper parts of soil. Its use is in places with high use, like a race-course, edges of roads or vineyards alleyway. Today, in time of global warming, its usage becomes more important for park purposes and low-input turfs, where is its higher resistance against biotic and abiotic stresses, mainly against drought, utilized. Festuca arundinacea complement well with Poa pratensis in turf.
Goal of this work was to evaluate turfs with Festuca arundinacea and chosen grass species under different mowing frequency. According to hypothesis frequency shouldn´t affect turf coverage. Coverage should be same with different mixture composition with Festuca arundinacea.
The experiment was performed on experimental land of Czech university of Life Sciences Prague, Suchdol in 2015. Mixtures of Festuca arundinacea with Festuca rubra, Poa pratensis and Lolium perenne were seeded together with monocultures of these species in 2012. Mixture composition was 50/50 except mixture of Fesruca arundinacea and Poa pratensis, where the mixture was 75/25. There were conducted 63 plots, in 3 repetitions and in 3 different frequencies of mowing (by 14, 30 and 45 days). Each plot was 6 m2 big. The results were evaluated by analysis of variance ANOVA (P is smaller than 0.05) in program Statgraphics, version XV.
Influence of mixture composition to level of growth was significant. The highest level of growth has monoculture of Festuca arundinacea (10,6 cm). The lowest level of growth has monoculture of Lolium perenne (5,4 cm). Monoculture of Festuca arundinacea had the highest intensity of grow (0,07 cm/day) at 14-day frequency of moving. Lolium perenne has the lowest intensity of grow (0,02 cm/day). At 30-day frequency of mowing mixture of Festuca arundinacea with Poa pratensis has the highest intensity of grow (0,24 cm/day). The lowest intensity of grow has Lolium perenne (0,01 cm/day). The highest intensity of grow was at 45-day mowing frequency. The highest intensity of grow has mixture of Festuca arundinacea with Festuca rubra (0,75 cm/day). The lowest intensity of grow has monoculture of Lolium perenne (0,06 cm/day). Influence of mixture composition to coverage was statistically significant. The highest coverage has mixture of Festuca arundinace with Festuca rubra (81,3 %). The lowest coverage has monoculture of Lolium perenne (58,6%). Influence of frequency to level of growth was statistically significant. The highest level of growth has 45-day frequency of mowing (6 cm) and the lowest level of growth has 14-day frequency of mowing (3,8 cm). Influence of frequency to coverage was statistically significant. The highest coverage 14-day frequency of moving (76,5 %) and the lowest has 45-day frequency of growing (66,7 %).
The knowledge gained will be used for further compilation grass mixtures for park lawns. It turned out that it is better to prefer seeding mixtures with Festuca arundinacea than monocultures. To achieve high coverage and optimal intensity of growth with Festuca arundinacea was recommended to follow 30-day frequency of mowing.
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