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Symposium molekulární biologie sinic /7./
Komenda, Josef ; Flores, E. ; Nixon, P. J. ; Waleron, K. ; Maršálek, Blahoslav
The conference was devoted to the recent advances in the field of molecular biology of cyanobacteria, namely in the structure and function of important proteins and their complexes, in the regulation of gene expression, in cell differentiation, in cellular metabolism and in molecular ecology and taxonomy. There were 170 participants presenting 101 posters and 12 invited and 29 selected lectures including a computer bioinformatics session
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Nanoporézní vrstvy TiO2 a jejich využití
Krejčíková, Simona ; Matějová, Lenka ; Cajthaml, Tomáš ; Matěj, Z. ; Strýhal, Z. ; Šolcová, Olga
Titania that is promising as catalytic agent for removing of organic impurities from waste water and air, photovoltaic cell and pigment, were studied. Doped Titania layers deposited on various substrates were prepared by sol-gel technique. Sol was prepared by using nonionic surfactants Triton X, organic solvent, water and metal precursors. Deposited gel layers were converted to anatase layers by calcination at 400°C for 4 hours or by subcritic H2O/ supercritic CO2 extraction/ subcritic CH3OH extraction (SWE/SFE/PFE). For deeper textural and structural characterization of prepared layers nitrogen physical adsorption, XRD, AFM and SEM analyses were performed.
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Příprava TiO2 pro fotokatalytické aplikace
Matějová, Lenka ; Cajthaml, Tomáš ; Matěj, Z. ; Strýhal, Z. ; Benada, Oldřich ; Šolcová, Olga
Powder nanoporous TiO2 with narrow monodisperse pore-size distribution in the range from 2 to 30 nm were prepared by self-assemble micelle system in nonpolar and polar solvents at laboratory temperature and pressure. Nonionic surfactants Triton X-102, 100, 114, 45, 15 were used for preparation of micelle systems. Various approaches were applied for removing of residual organic matter from prepared titania gels: a) calcination, b) improved extraction by supercritical (CO2) and subcritical fluids (H2O, CH3OH) and c) the combination of both forenamed approaches.
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Vliv rekombinantní bakterie Lactobacillus plantarum na vývoj pylové alergieL
Schwarzer, Martin ; Repa, A. ; Wiedermann, U. ; Hrnčíř, Tomáš ; Daniel, C. ; Pot, B. ; Štěpánková, Renata ; Hudcovic, Tomáš ; Tlaskalová, Helena ; Součková, Martina ; Kozáková, Hana
Germfree mice were monocolonized with recombinant Lactobacillus plantarum producing Bet v 1. Subsequently mice were sensitized by intraperitoneal injections to Bet v 1. We found out that monocolonization with recombinant L. plantarum reduced the level of specific Bet v 1 specific IgE antibody and stimulated production of INF-gamma in spleen cells supernatants. We conclude that monocolonization by recombinant L. plantarum shifts the immune response towards Th-1 direction and that it is a promising vaccine candidate against type I allergy
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Fluorescenční kinetická mikroskopie: 2D zobrazování variabilní fluorescence chlorofylu jednotlivých buněk řas se spektrálním rozlišením
Prášil, Ondřej ; Šetlíková, Eva ; Veselka, D.
The fluorescence kinetic microscopy (FKM) provides new dimension in application of chlorophyll fluorescence techniques. It combines classical fluorescence microscopy with 2D kinetic detection systems. FKM allows to examine photosynthesis of individual algal cells or on the cellular level of macroscopic structures. FKM is well suited to study heterogeneity in photosynthetic response among individual cells in algal cultures or in natural mixed populations. Special microscopic chambers allow to maintain live cells under optimal conditions for several hours or days and to follow evolution of photosynthesis in individual cells during the course of their growth cycle. Recent technical developments have allowed to determine FKM fluorescence kinetic with spectral resolution
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