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Chladová odolnost hmyzu a sezónní změny ve složení sterolů a tokoferolů
URBAN, Tomáš
Předkládaná práce se zabývala zkoumáním chladové odolnosti u hmyzu (Pyrrhocoris apterus), zvláště pak kvalitativním a kvantitativním složením a sezónními změnami v obsahu sterolů a tokoferolů v membránách. To zahrnovalo sběr jedinců, extrakci tkání, separaci, připravu vzorků a jejich analýzu. Podařilo se detekovat tři různé steroly (živočišný cholesterol, rostlinný kampesterol a sitosterol). Dále byly zjištěny izomery ?-tokoferolu a ?-tokoferolu. Obsah cholesterolu byl v rozmezí 0,2 - 0,8 molárních %, u fytosterolů bylo rozmezí 1,2 ? 15,2 molárních % a u tokoferolů bylo rozmezí 0,1 ? 31,3 molárních %. Nakonec bylo vyhodnoceno sezónní kolísání sterolů a tokoferolů s minimem v létě a s maximem v zimě.
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Transport of divalent cations across the gel supported phospholipid membranes
Navrátil, Tomáš ; Šestáková, Ivana ; Mareček, Vladimír
This contribution deals with elucidation of principles of transporting processes of heavy metals (mainly lead, cadmium) across the biological membranes. The real membranes were for purposes of described experiments, replaced by model membranes, which were composed of phospholipid bilayers. Two different phorpholipids (1,2-dipalmitoyl-sn-glycero-3-phosphocholine and the mixture of phospholipids obtained from soybeans (under commercial name Asolectin)) were used as the building elements for the formation of these membranes on the surface of the constructed gel electrode. The registered transporting processes have been characterized using voltammetry, and electrochemical impendance spectrometry (EIS). The impact of some parameters on these systems and processes was investigated.
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Purification of Biogas by Means of Membranes
Poloncarzová, Magda ; Vejražka, Jiří ; Izák, Pavel
The aim of this work is to find a way to lower carbon dioxide concentration in biogas and to purify it from undesirable gases by means of membrane. Ideally only one or two steps should be used to make the technology feasible and profitable. Liquid membranes are used because of their high selectivity and good permeability. Successful biogas purification method would open new opportunities in ecological fuels, especially if the methane content in biogas reached more than 95%; biogas could then replace the natural gas. In this work, a new supported ionic liquid has been tested and the first results have been obtained. Sweeping gas permeation was performed with model mixture of CO2 and CH4 1:1.
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Transport of cadmium ions across model supported phospholipid membranes
Navrátil, Tomáš ; Šestáková, Ivana ; Mareček, Vladimír ; Štulík, Karel
These results report on experiments focussed on the formation of supported model phospholipid membranes, formed from phosphatidyl choline in the micrometric pores of hydrophilic polycarbonate supports, their chatacterization using voltammetry and electrochemical impendance spectrometry (EIS). Attention was paid transport of heavy metal ions (ions of cadmium, etc.) across these membranes in the presence or absence of ionophores (valinomysing and calciomycin) or in the presence of oxalic acid. The impact of some parameters on these systems and processes was investigated.
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