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Bilrubin - physicochemical properties and biochemical importance
Kupcová, Kristýna ; Hudeček, Jiří (advisor) ; Martínková, Markéta (referee)
This thesis briefly summarizes the literary data concerning bilirubin. In addititon to this, its experimental part is devoted to comparison of stability of bilirubin and its derivative, ranarubin, towards photolysis in aerobic conditions. The photolytic experiments were carried out at wavelength 366 nm for 4 hours. The resulting photolysates were characterised by absorption spectroscopy (400 - 800 nm) and mass spectrometry. The data obtained show a high degree of similarity for both compounds, which are both relatively stable against photolytic splitting and photooxidation to biliverdin.
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Study of thiol addition to biliverdine, the synthesis of labelled bilirubine
Felklová, Veronika ; Lešetický, Ladislav (advisor) ; Smrček, Stanislav (referee)
Biliverdin and bilirubin are bile pigments which are degradation products of heme. Biliverdin (BV) is greenish-blue pigment and is reduction product of tetrapyrrolic core of heme by influence of the hemoxygenase (HO). The final product of this degradation is yellowish-brown pigment bilirubin (BR) which forms from BV thanks to the biliverdinreduktase (BVR). Normal and slightly raised level of bilirubin in plasma has cytoprotective effects whereas high levels are cytotoxic often. In severe unconjugated hyperbilirubinemia cases (newborn children) unconjugated bilirubin (UCB) accumulates in central nervous system (CNS) and causes bilirubin-induced neurologic dysfunction (BIND). Unfortunately there is a limitation for finding UCB pathophysiology caused by difficult determination of UCB content and distribution in tissues and biological fluids. So the main purpose of this thesis is to find and integrate isolated methods which will serve as the basis of finding bilirubin distribution. This progress would have a significant effect on studies of bilirubin neurotoxicity on newborn children. This method is based on radioactive labeling of UCB. Preferentially atom C 10 used for binding suitable functional group (thiols) because conformation of indicated bilirubin shouldn't change in this position. And then the...
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Development of new method for the determination of bilirubin and its esters
KAMIŠOVÁ, Tereza
This work describes present knowledge of bilirubin, its metabolism in human body and attributes necessary to its determination by high performance liquid chromatography (HPLC). Its similarity with phycobilins is summarized. Bilirubin nonpolar esters could be used to prepare self-assembling light-harvesting antennas. The experimental part involves modification of a mobile phase for HPLC determination of bilirubin and its esters, which were synthesized by Fisher esterification.
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Nursing process in newborn with hyperbilirubinemia
SEKALOVÁ, Monika
The thesis deals with Nursing process with neonates diagnosed with hyperbilirubinemia. The theoretical part focuses on the physiological and premature new-born babies, anatomy and physiology of blood, neonatal hyperbilirubinemia problems, nursing process and NANDA and NIC classification systems. The practical part contains research results. Qualitative method was used for the research and it was carried out at the neonatal department in Nemocnice Ceske Budejovice, a.s. (Ceske Budejovice Hospital). The first objective was to determine the specifics of nursing care for full-term new-borns and premature new-borns diagnosed with hyperbilirubinemia. It was found that the specifics were mainly in monitoring of the physiological functions, measurement frequency of transcutaneous bilirubinometry, in positioning the new-born during phototherapy, in the prescribed temperature inside the incubator and in children's diets. All information necessary was collected through interviews with nurses, from documentation content analysis and by observation. Based on the information, case studies were developed. The research sample consisted of two new-born babies diagnosed with hyperbilirubinemia. The second objective of the thesis was to determine nurses´ position when using NANDA classification systems to diagnose Neonatal jaundice and NIC Phototherapy: new-born. It was found that nurses would change some defining characteristics and related factors to diagnose Neonatal jaundice (00194) as well asinterventions from NIC Phototherapy: new-born (6924). Most of them agreed that they would not like to use these classifications during care of the new-borns. Information was collected using structured interviews with three neonatal nurses and two midwives. The results could be used to improve nursing care for neonates diagnosed with hyperbilirubinemia.
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