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The effect of IDGF2 protein on expression of selected genes in cell line Cl.8+
ROUHOVÁ, Lenka
The imaginal discs growth factors (IDGFs) are proteins able to induce proliferation of Cl.8+ cells proliferation in collaboration with insulin. They are also connected with response to starvation stress and innate immunity. A genome-wide analysis of Cl.8+ cells treated with IDGF2, which was performed in our lab, detected tens of genes transcriptionally induced by this protein. The aim of this work was to confirm this effect with some of these genes using quantitative PCR.
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Methodology for the phenotyping resistance to Plum pox virus in peach
Salava, Jaroslav ; Polák, Jaroslav
Sharka disease, which is caused by the Plum pox virus (PPV), is the most economically important virus disease in fruit trees worldwide. Since it is not possible to completely eradicate PPV in the Czech Republic, it is necessary to find another way to control this pathogen. The most promising seems to be the breeding of new cultivars for resistance. This methodology provides a comprehensive protocol for testing PPV resistance in peach. It is based on visual assessment of disease symptoms on leaves and fruits, and determining the presence of the virus in plants using ELISA and RT-PCR techniques.
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Methodology of hop latent viroid (HLVd) diagnostics
Svoboda, P. ; Patzak, J. ; Matoušek, Jaroslav
Viroids belong to the pathogens, which cause important economic losses in quality and quantity of hop cones. Hop (Humulus lupulus L.) as a perennial crop generally grown for nearly twenty years at the same site is seriously endangered by viroid infection. A foolproof diagnostics of viroid infection is necessary for the assessment of healthy state in hop plants.Molecular hybridization (dot-blot) and RT-PCR methods enable a reliable determination of latent hop viroid (HLVd). The methodology consists of the diagnostics as well as its utilization within the evaluation of hop healthy state.
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Methodics of molecular detection of Barley yellow dwarf virus in vectors by RT-PCR
Jarošová, Jana ; Jaňourová, Blanka ; Kumar, Jiban
Barley yellow dwarf is one of the most important viral diseases of cereals in the world. It can cause significant yield losses in most cereal species, including wheat, barley, rice, corn, oat and rye. In this methodology we provide assays for reverse transcription - polymerase chain reaction (RT-PCR) for sensitive detection of barley yellow dwarf virus directly in the vectors, in aphids. This way it is possible to evaluate infectivity of vectors and from that conclude subsequent measures. The methodology is meant to serve the employees of State Phytosanitary Administration.
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Methodology of resistance evaluation of plum, Prunus domesica L., clone C5 to the Plum pox virus and mixed infections with another two viruses
Polák, Jaroslav ; Kumar, Jiban ; Jarošová, Jana
The methodology encloses the procedure of preparation and ínoculation of transgenic plants of plum with the individual viruses, the maintenance of field orchard, the evaluation of virus symptoms in leaves, serological and electronmicroscopical detection of viruses in leaves of plum trees, determination of relative concentration of PPV by DAS-ELISA, detection of PPV-Rec, ACLSV and PDV by RT-PCR, and determination of risk of recombinations between PPV-Rec and the homologous transgenic transcript in P. domestica, clone C5.
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