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Study of the composition and organization of cytochrome P450 system by covalent crosslinking
Koberová, Monika ; Hodek, Petr (advisor) ; Hansíková, Hana (referee)
The system of mixed function oxygenase (MFO system) participates in significant roles in the metabolism of endogenous compounds and xenobiotics. This system contains cytochrome P450, NADPH:cytochrome P450 reductase, and also there are assigned NADH: cytochrome b5 reductase and cytochrome b5. It was proved that cytochrome b5 can stimulate or inhibit cytochrome P450 (CYP)-dependent reactions and even change the ratio of resulting metabolites. The mechanism of cytochrome b5 action has not been fully elucidated yet. Elucidation of protein-protein interactions in MFO system and determination of topology of this system could explain the mechanism of cyt b5 action. The covalent cross- linking technique is suitable method for identifying protein-protein interactions within the membrane. Cytochrome b5 contains 3 methionines and in 2 cases the methionines are localized in a short hydrophobic C-terminal membrane anchor. Interactions with cytochrome P450 in the membrane environment can be identified by substitution of two methionine for photoactivatable analogue of methionine (photo-methionine) and subsequent photoactivation. This work is focused on expression and isolation of photo-cytochrome b5 (photo- cyt b5), cytochrome b5 analogue with incorporated photo-methionine. Conditions for photo-methionine...
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Serge Reggiani, the importance of inconspicuousness
Hrzina, Václav ; VALAK, Radim (advisor) ; Slavíková, Nataša (referee)
I would like to understand the importance of an actor in a movie. I choosed Serge Reggiani, a french actor, who never became a star number one. Reggiani went through a lot of important movies in principal or small roles. What it really means acting in a movie, what brings freedom to an actor in front of a camera and is one actor able to play different characters in every other movie. A diversity and length of his filmography, different directing styles he went through will help me to concretize attitude of an actor and perception of his „different“ characters by audience. I would like to bring up some acting metodes in cinematography in my work too.
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Mask, Disguise and Role Play in Worldwide Contemporary Self-Portrait
Helfertová, Žofie ; SILVERIO, Robert (advisor) ; DVOŘÁK, Tomáš (referee)
This bachelor thesis deals with the role of mask, disguise and playing with the roles through self-portrait in worldwide contemporary photography. In the first chapters it follows the general development of the self-portrait and a brief history of photography and its forms and tendencies from beginning to the present, it is also focused on the mask in general and role of masks and disguises in the history of photography. Main part of this work is the last chapter, which is devoted to the youngest generation of contemporary photographers. In their self-portraits we can follow tendency which reveals use of mask and disguise for change of their identity. However, they do not create a new identity but takes over, or form the illusion of existing already defined identities. The aim of this thesis is to present the mask as an instrument for role play through a self-portrait.
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The Role of Nurses in Colorectal Surgeries
ŠULCOVÁ, Jana
Theoretical basis: The problems in rectal and anal diseases have had rising tendency, especially haemorrhoidal diseases, on which we put emphasis in our bachelor thesis. 37% of adult population suffers from this disease so we may line it up to civilization illnesses. Their treatment involves wide range of medical methods, from the conservative therapy to semi invasive ways of treatment. The semi invasive treatment is well withstood by patients and it can help up to 90% of clients. This bachelor thesis is concerned with specifics of nursing duties in a colorectal surgery. It links with topics like taking faecal specimen, basic methods of endoscopy, communication with patients and giving them information about lifestyle. The theoretical basis describes haemorrhoidal disease and gives brief introduction into causes of its developing, most common symptoms and possible treatments. The aim of the thesis: From the practical point of view we set two tasks. The first one is aimed at specifics of nursing job in a colorectal surgery and the second one tries to monitor how nurses in colorectal surgeries inform patients about healthy lifestyle. We asked two research questions regarding these aims. The first research question is: What are the specifics of the nursing job in the colorectal surgery? The second question concerns how nurses in colorectal surgeries provide information to their patients about healthy lifestyle. Methodology: Regarding the bachelor thesis we decided to choose a form of a qualitative research with using semi structural interviews with practical nurses working in colorectal surgeries. The interviews took place from February 28 2014 to March 15 2014 in five colorectal surgeries in following towns: České Budějovice, Písek, Strakonice, Jindřichův Hradec a Třemošná u Plzně. The focus group was created of 6 nurses working in colorectal surgeries. The interviews were performed and recorded with the consent of all practical nurses involved in the research. All extracted data was arranged and categorised into groups that were consequently analysed. The interviewer´s questions were prepared in advance and some extra questions were asked during the interview. The interview was divided into five areas: Identification questions, role of a nurse, examination methods, recommendation and giving information about lifestyle. Results: The results show the specifics of nursing in colorectal surgeries. We found out that nurses working in colorectal surgeries deal mainly with preparing special medical instruments and devices needed for endoscopic examination. Then they assist doctors during a medical examination and with treatment methods of RBL (Barron´s rubber band ligation) and Hemoron, they manipulate with devices in a surgery, check hygiene measures, do administrative work and keep surgery office running. In other conversation areas practical nurses mostly mentioned sampling of biological materials, mainly blood and faecal specimen taking to be given up to histological examination, preparing patients for an endoscopic exam, communication with patients and attending to the intimacy of patients. Consequently we found out that more than half of the practical nurses pay attention to psychological conditioning of the patient before rectoscopy. The research also revealed some weak spots. More than half of the nurses in the focus group do not inform patients about preparations before anoscope exam. Barely one third of the group said that they inform patients about the natural emptying before the exam. Regarding the issue of giving information about lifestyle, we came to the conclusion that practical nurses, even though they do not have enough time, try to inform patients about physical activities, hygiene care and quality of eating and they do it in sufficient rate. But because of the time constrain they put emphasis only on the essential information.
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Comparison of cytokine production by leukocytes from newborns of healthy and allergic mothers
Dusilová, Adéla ; Hrdý, Jiří (advisor) ; Janatková, Ivana (referee)
The increasing incidence of children suffering from allergic diseases could be caused by sensitization of immature immune system during the intrauterine development. Several important scientific papers have demonstrated the ability of cord blood cells to respond by elevated proliferation activity after stimulation by common allergens. Following these findings, present study follows the production of cytokines which play a role in the pro- and anti-allergenic tuning of the immune system. Umbilical cord blood cells were stimulated with polyclonal activators (phytohaemagglutinin) and common allergens (ovalbumin, timothy grass, birch, mite). Subsequently, cytokine production was monitored using selected methods that reflect different stages of cell activation - at the level of mRNA by quantitative real time PCR (qRT-PCR), by flow cytometry detection of the presence of intracellular cytokines in different cell subpopulations and by ELISA measurement of cytokines in CBMC culture supernatants. The results obtained point to a very weak ability of these common allergens (timothy grass, birch, mite, ovalbumin) to stimulate CBMC to produce cytokines observed by all of these methodological procedures. Although we did not observe significant differences in CBMC cytokine production (IL-2, IL-4, IL-10, IL-12,...
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Role of Psb28 proteins in the biogenesis of the Photosystem II complex in the cyanobacterium Synechocystis sp. PCC 6803
BEČKOVÁ, Martina
The thesis focuses on the role of Psb28 proteins, namely the Psb28-1 and its homolog Psb28-2, in the biogenesis of the Photosystem II complex (PSII) in the cyanobacterium Synechocystis PCC 6803. The aims of this work were to localize the proteins within the cells, and to determine their function. A fraction of both Psb28 proteins was identified in the monomeric PSII core complexes but most proteins were found in the unassembled protein fraction associated with thylakoid membranes. Psb28-1 was mostly detected as a dimer while Psb28-2 as a monomer. Psb28-1 also differed from Psb28-2 by its higher affinity to the PSII core complex lacking CP43 antenna. Characterization of Psb28-less mutants suggested regulatory function of the proteins in PSII biogenesis in connection with chlorophyll biosynthetic pathway. Analysis of preparations isolated using FLAG-tagged versions of Psb28 proteins showed their association with Photosystem II - Photosystem I supercomplexes, especially under increased irradiance, and supported a role of Photosystem I in the PSII biogenesis.
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Nursing care for long-term bedridden patients with urinary incontinence.
KUBÍKOVÁ, Nikola
Bachelor thesis titled ,,Nursing care for long-term bedridden patients with urinary incontinence" deals with the nursing care of clients with urinary incontinence has long restrained on a bed on the aftercare department. Urinary incontinence is a condition of involuntary leakage of urine, which is a major health and social problem (Kalvach and team, 2004). According to the International Continence Society (ICS) urinary incontinence is classified into extraurethal and urethral. Extraurethal incontinence can be divided into innate and fistulas. The congenital disorder contains atrophy and ectopic urethra. We can divide fistulas into uretral, vesicular and ureteral fistulas. Urethral incontinence are further divided into stress, reflex, urgent and paradoxical ischurii (Locksmith and Macek, 2012). From a nursing perspective is urinary inkontinence divided into nursing diagnoses : Functional incontinence 00020, then Urinary incontinence with overflow 00176, Reflex incontinence 00018, Stress urinary inkontinence 00017, Urge urinary incontinence 00019, Impaired excretion of urine 00016, Urinary retention 00023, Risk of urge urinary incontinence 00022, Efforts to improve the excretion of urine 00166 (Herdman, 2013). The theoretical part of the thesis is talking about the definition of the concept of urinary incontinence and its further division. Also is solving the role of nurses in the diagnosis of myocardial infarction, therapy, nursing process for clients with urinary inkontinence and classification of urinary incontinence by nursing diagnoses with nursing interventions. The practical part of my thesis contains the results of qualitative research, which were obtained by interviews, in-depth interview technique, then content analysis of nursing documentation and comparison of two standards of nursing care of selected hospitals of the Sought Bohemia. The practical outcome of the thesis will be finished client's care plan which is dealing patient long-term bedridden with urinary incontinence.
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The Media Influence on Transformation of Democracy
Půlpánová, Barbora ; Pehe, Jiří (advisor) ; Slačálek, Ondřej (referee)
Mass media has always played the key role in democratic process. They are not only the indicator of its right functioning, but also the iniciator. From historical point of view is obvious, that technological progress and changes in the way of informing people were related to the political area. But with the increasing role of media, they changed their position from pure mediators of informations to the main actors of social and political life. The ‚old' media, such as newspapers, radio and television, are therefore partly responsible for commercialization of the public sphere and also for its depolitization. With contribution of the globalization effect on today's world, which causes besides other things the weakening of the nation state, there are only a little of the key democratic aspects left. Some find the solution in the ‚new' media represented by the Internet and social media, which are more interactive so they could get the citizens back to the game. And right in the increase of civic engagement see the theorists the potencial for creating a new democratic order based on high-level participation. KEY WORDS Mass media, Democracy, Public Sphere, Civic Engagement, Internet, Social media, Participative Democracy, Deliberative Democracy
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Evaluation of the effectiveness of teaching vocational subjects depending on physical activity before schooling.
Janošíková, Martina ; Hanušová, Marie (advisor)
The period of transition to secondary school brings substantial changes in the requirements for study obligations, autonomy, communication, expression, or separated from their families in the form of staying at the dormitory. Students enrolled in sport centers, in addition to these changes, still struggling with the responsibilities associated with sport. Because they are students first through fourth grades of secondary schools and secondary vocational schools, an age when image self-I am, what I believe in, arises in interactions with the outside world and their own experience, plays a very important role to influence the environment, educators, teachers and trainers, with which students spend most of their time in practice. This study looks at students comprehensively in terms of personal development as a multifactorial biopsychosocial unit, in terms of cognitive and motivational processes, states and properties from the perspective of social psychology dealing with the interactions, relationships and communication, psychology of individuals within the social group and its importance for the development of the individual . It monitors conditions and educational outcomes in terms of educational psychology, dealing with forming properties, opinions, attitudes and value orientation from the perspective of educational psychology.
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The occurrence of domestic and wild forms of the camels in the old world and their economic use
Burgerová, Michaela ; Gardiánová, Ivana (advisor) ; Jan, Jan (referee)
Genus camel (Camelus) includes two species, the Dromedary camel (Camelus dromedarius) and Bactrian camel (Camelus bactrianus). Camels have a wide range of utilization. People in Arabia countries but also in India and Egypt are using camels as a milk and meat animal, or even as a transport and companion animals and riding animals too. The aim of this thesis was to find sources about camel keeping and using and his role of farm animal in different cultures and countries. And about conditions and requirements in captivity. The first part of thesis contains information about phylogeny and origin of the camel family, taxonomy of camels, short information about the history of domestication and using of camels. There are also described the two species in terms of species biology etc. plus a brief mention of camel hybrids and camel breeds. The second part focuses on the use of camels. The preffered and most common use is in the production of milk, other dairy products from camel milk, fermentation of milk. Another use is for meat performance, meat composition, describes products. His utilization i also useful for other parts such as hair wool and mane, they come mainly from bactrian camel (Camelus bactrianus). The last is working utilization of camels, carrying baggage. Then hobby use. The thesis describes the conditions of keeping camels in captivity, keeping camels in Somalia, requirements for the supply of water and food, housing conditions in the Czech Republic, it is particularly widespread keeping camels in zoos. In Czech Republic they are small private camel breeders. For comparison they are conditions for keeping camels in captivity in Australia.
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