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Card of Cryptosporidium infections by humans and animals
BLÁHOVEC, Ondřej
The representatives of the Cryptosporidium genus are one of the causes of gastrointestinal tract diseases in humans and animals. In animals the host may even be a respiratory apparatus. The disease is called cryptosproridiosis. In majority of cases this infection can bypass without any major problems, but in immunosuppressed individuals it can cause serious health problems. Cryptosporidium has a monoxenous development cycle, which basically means that the entire development takes place in a single host. Exogenous stage is represented by oocysts, which are in case of a gastrointestinal disease excreted in faeces. In case of a respiratory disease the oocysts make they way out via respiratory and nasal secretions. This leads to contamination of the environment or water. In general, it is expected that Cryptosporidium isolates, which are present in one class of vertebrates, are not infectious to a non-specific host from other classes. It is also expected that cryptosporidia have low host specificity. But this does not exclude that some kinds have gradually extended its specificity to more species. It is also apparent that cryptosporidium infections are common in animals that inhabit the external environment, so even a human can be endangered by this zoonosis, although the incidence in the Czech Republic is low. The reason for the low numbers may as well be that parasitological examination is not performed very often, so the estimated prevalence in the population is probably much higher. Therefore to reveal the originator of this disease it would be appropriate to perform a parasitological examination in persons who are in contact with animals, this way the cryptosporidium infection would be excluded or proven.
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How to prevent the proliferation of Legionella pneumophylis in hot water in the hospital Czech Krumlov
TUREK, Jan
This diploma thesis deals with microbiological quality of hot water in a hospital in Cesky Krumlov as well as hot water piping condition with respect to Legionella. It also deals with arrangements for improving the quality of hot water in this hospital. The subject and target of this thesis are the findings of the mentioned parameters based on own observations, technical documentation and analysis of warm water performed by an accredited laboratory. The hospital in Cesky Krumlov, the construction of which began in 1909, was built in 1911 as the 'Nemocnice Císaře Františka Josefa I.' As the time went by, new buildings were built up. The hospital was renamed to 'Všeobecná veřejná nemocnice' in 1942. In the following years the hospital expanded until the current condition. Water quality and presence of Legionella is a world-wide issue, which we must fight against. The thesis is divided into two parts. First, theoretical part, which includes both general and specific knowledge about Legionella, its health risks, the environment in which it occurs, possibilities of eliminating, suitability of the material for hot water pipes, gained in professional literature and a part of the Czech legislation dealing with public health protection, the law 'No. 258/2004 Coll.' in actual version and quality of drinking water and hot water. In the second, empirical part, I focus on the situation of the hospital in Cesky Krumlov from 2009 till 2015, concerning newly built boiler room and the whole distribution system of hot water regarding Legionella. Particularly I focused on materials used for ecologisation of the gas boiler room and materials used for hot water pipes and actual state of water distribution in individual buildings of this health care facility. I mentioned the analysis of the water samples performed by an accredited laboratory with corresponding technical documentation, which concerns the water distribution system, provided by the medical facility. I used secondary data analysis to evaluate these documents. Regarding the results from 2009 to 2015 the microbiological quality of warm water doesn't meet the limits defined in the Attachment No. 3 of the above mentioned law Order 252/2004 Coll. Limit value for colony forming units of Legionella is set to maximum of 100 CFU. In most of the samples this limit was exceeded by series of units up to hundred thousands of units. The last results in 2015 prove improvement of the unfavorable situation. In that year the analysis was tested three times. First in February, where the CFU units were 'only' in hundreds, with the top value of 550 CFU in the Postacute Care ward. Second analysis was performed in June that same year. Results were acceptable. The highest value was 64 CFU. Last analysis in the hospital was performed in December. Increased concentration of Legionella was again in the Surgery building. There were established of 1400 colony forming units. There were 300 CFU in the Postacute Care ward and the limit value of 100 CFU was in the ward of Internal medicine. The answers to research questions 'How to prevent reproduction of Legionella peumophylis in the water distribution system of the hospital in Cesky Krumlov?' and 'How to ensure a sufficient amount of quality hot water in the medical facility?' are included at the end of the stated recommendations. A risk factor causing the fast reproduction of Legionella is the old water distribution system in most of the buildings and low water temperature of water leaving the boiler, which is 55°C. All mentioned results and evaluations are included in this thesis as well as attached pictures, which show the environment and situation within the Health Care Institutions.
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Účinky xenobiotik na oxidační stres, metabolizmus lipidů, integritu DNA a životaschopnost lidských buněk a rybích spermií in vitro
LINHARTOVÁ, Pavla
Pollution of the aquatic environment by inorganic and organic chemicals is a major factor posing a serious threat to the survival of aquatic organisms including fish. In addition balancing risks and benefits of fish consumption is nowadays an intensively discussed public health topic. Spermatozoa of almost all fish species are released into water environment where they can be directly exposed to various compounds, such as xenobiotics including toxic metals, prior to fertilization. In addition, exposure of parental adults to various xenobiotics may affect gamete quality, which may subsequently reduce fertilization success. On the other hand the advantages of eating fish are well-known, not only in the point that fish is a healthy source of protein and other nutrients, but eating contaminated fish may also confer various health benefits. Research over the past few decades has shown that the nutrients and particularly the n-3 fatty acids (FA) found in fish and seafood, are for examples protective against cardiac diseases and have a positive impact on brain development. The thesis provides a focus on two different cell model types. Firstly, human hepatocellular cells (Hep G2, ATCC) were used as in vitro tool for studying the effect of the intake of cadmium (Cd2+) contaminated fish on cytotoxicity, oxidative stress and fatty acid and phospholipid class compositions. Secondly, spermatozoa of one threatened species of fish, sterlet (Acipenser ruthenus) were used as in vitro model for studying effect of potentially hazardous xenobiotic compounds' occurring in open waters. Sperm from sterlet were exposed for 2h to environmentally relevant concentrations of DQ (0-150
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Nové postupy biomonitoringu cizorodých látek ve vodním prostředí
ČERVENÝ, Daniel
Within the presented work, several approaches of biomonitoring were studied. A well-established method for human health risk assessment was applied to evaluate the quality of fish from open waters in the Czech Republic. As this work is targeted on sport fishermen, who frequently consume their own catches, besides the publication in scientific journal, a brochure with results of this study was distributed via the Czech Fishing Union. Health risks for consumers related to the consumption of wild fish were found strongly species and locality dependent. As there is no risks related with the consumption of carp (Cyprinus carpio) at all investigated sites, frequent consumption of predatory fish should be avoided at some fishing grounds. The use of passive samplers has become more and more attractive in the last two decades. As these devices are able to mimic the biological uptake of chemicals, their potential for replacing fish as bioindicators in routine biomonitoring programmes is evident. In our study, the comparison between analysis of fish tissues and POCIS extracts from selected sampling sites confirms this idea about interchangeability of these indicators in the case of PFASs as target pollutants. Moreover, no metabolic transformation of contaminants present in water take place in passive samplers contrary to living organisms, thus these reflect the actual contamination at the locality more precisely. The use of passive samplers completely fulfils the internationally accepted principles of Replacement, Reduction, Refinement (the three R´s). Another promising approach using YOY fish as bioindicators was evaluated within the present study. Multispecies samples of YOY fish homogenate was found as a better indicator for wide range of pollutants than muscle tissue of adult fish within one species from the same locality. As all inner organs and tissues are presented in analysed samples, more target pollutants in higher concentrations are detected compared to the muscle of adults. Besides the higher sensitivity in pollutants detection and quantification, practical and economic benefits are of concern in the case of the YOY fish approach. The YOY fish approach also particularly fulfil the three R´s strategy, as sampling of this age group of fish has a smaller impact on the environment than the sampling of adults. In case of mercury, that was found as a limiting contaminant for the consumption of fish from open waters in the Czech Republic, a nonlethal method of finclips sampling can be used for the monitoring of this compound in aquatic environments. Besides the monitoring, our optimised method for the estimation of muscle tissue concentrations could be used for human health risk assessment as well. The difference between real muscle concentrations and concentrations estimated using our method differs less than 10% in most of the investigated localities. Benthic organisms are an important part of the food web in aquatic environments, but insufficient information about its contamination is available. In another study included in this thesis, first evidence about bioaccumulation of certain pharmaceuticals in benthic organisms was described. Although, pharmaceuticals are generally considered to be not accumulative in organisms, our study proved that certain pharmaceuticals have bioaccumulation potential. From this finding, it is evident that not only the concentrations of pharmaceutical dissolved in water but also the diet represents an important exposure pathway of contamination by these compounds for organisms (fish) inhabiting aquatic environments.
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Occurrence of PBDE compounds and Bisphenol A in rivers with the occurrence of cryfish
Svobodová, Kateřina ; Vach, Marek (advisor)
The thesis deals with result evaluation of a project under the auspices of the EAA and Norway Grants, which ran from 2015 to 2016. The project's goal was to evaluate water quality at sites with native and invasive crayfish. The project also included monitoring organic substances such as Polybrominated diphenyl ethers (PBDEs). I mainly focused on a subset of PBDEs, the brominated flame retardants (BFRs). These substances have been monitored since the 1970s, when food was accidentally contaminated leading to the contamination of the nearby area in the US. What is alarming is their persistence in the environment, which leads to their constantly increasing levels. Information about the transformation and biodegradation of PBDEs in the environment is still limited and is continually analyzed in new studies. These substances are poorly volatile and insoluble, which makes dust and particles arising from the products containing BPAs the main sources of contact. Another agent, whose presence in the environment of the Czech Republic I analyzed, is Bisphenol A (BPA). BPA is used in the production of epoxy resins and polycarbonate plastics. BPA is a toxic substance with the ability to disrupt hormone function. Baby feeding bottles have been confirmed to contain these compounds. In April 2004, the Czech government approved a (program to reduce pollution of surface waters by dangerous harmful substances). In August 2004, the EU enacted a ban on the sale and use of penta-BDE and octa-BDE in all fields. Since 2011, Bisphenol A may not be used in the manufacture of baby feeding bottles. The goal of this thesis is to analyze and evaluate the amount and possible consequences of these substances in our environment, which are alarming even after full or partial bans on the use of these substances.
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Scattering of Sun Radiation on Microparticles in a Closed Environment of Water Bath
Jelínek, Marek ; Pecen, Josef (advisor) ; Zeman, Josef (referee)
This thesis deals with an alternative technology used to illuminate spaces without access to daylight. Technology uses primarily scattering and also reflection and refraction of sunlight in the optical environment of the PET bottles with water and microparticles. The base of technology consists of a plastic bottle filled with water with microparticles and positioned so that it vertically penetrates the roof interior. The effect of scattering of sunlight in the bottle is amplified by using a suitable material of the microparticles.
Review of literature deals with areas that the technology is primarily designed for and the current state of expansion. The principle of technology and factors affecting its efficiency are explained.
The aim of the experiment was limited testing and selection of suitable material of microparticles and their concentration for the possibility of practical use within the technology described. Several measurements were performed of the intensity ratio of incident and scattered light using selected materials of microparticles of various concentrations. Based on the obtained values and their comparison a solution containing 0.5 g of limestone into 1.5 l of water was selected as the most suitable optical environment for the solution.
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Postproject analysis of the Plzeň highway bypass
Borkovec, Martin ; Martiš, Miroslav (advisor) ; Karel, Karel (referee)
First part of diploma thesis outlines road transport, its development and social significance. It describes issues of road transport in relationship to environment and influence on human health. It further informs about process of assessment influences on environment (EIA) and about post-project analysis of influences to environment. Discusses goals and issues within these two interdependent processes.
Second part is focused on the city Pilsen itself and its constructed motorway bypass. It evaluates efficiency of applied measures moderating negativ impact of construction on evironment and human health. Further examines efficiency of motorway bypass, if it fulfilled its purpose. So, whether managed divert significant part of transport from motorway D5 outside city Pilsen. Other area of research is noise and emission ballast around previous motorway route through the city Pilsen.
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Use of urban greenery for lecturing on biology, forestry and environment
Šlechta, Rostislav ; Kuneš, Ivan (advisor)
This thesis aims to develop the concept of use of urban greenery in Znojmo for lecturing on biology, forestry and environment. Perform basic records of trees selected in urban green area; identify the target group of pupils and suggest appropriate topic fieldwork in accordance with the content of the curriculum taught. Choose a suitable, safe excursion route, develop a concise educational materials for teachers and propose a method for testing the effectiveness of fieldwork.
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Impacts of anthropogenic pollution of lead on the environment
Grund, Daniel ; Francová, Anna (advisor) ; Ouředníček, Petr (referee)
The scope of this bachelor´s thesis is to define the lead as a toxic element, its occurence and impact on the environment (water, soil, atmosphere), the causes and effects of pollution on living organisms, to summarize information of bio-indication and bio-indicators and describe the method for detection metal concentrations in environment from collected specimens of mosses, lichens and snow in the Ostrava area. Part of this bachelor´s thesis is an experimental evaluation of the environment by using the measured concentrations of lead and comparing the measured values with other authors.
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