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Utilization of light scattering techniques in study on protein denaturation
Köbölová, Klaudia ; Kalina, Michal (referee) ; Sedláček, Petr (advisor)
This bachelor thesis is focused on verifying the use of light scattering techniques in the study of protein denaturation. The theoretical part of the thesis describes proteins, their general properties, the process of denaturation and it provides an insight into light scattering. In the experimental part of the study I investigated the denaturation effects of four selected potential denaturing agents – urea, guanidine hydrochloride, sodium dodecyl sulfate, copper (II) sulfate – on two model enzymes: lipase and lysosyme. The denaturation effects of these denaturants were studied with the help of the dynamic light scattering method (DLS). In order to describe the change in particle size during denaturation, I used Z-Average, Volume mean and intensity distribution which were determined by this method.
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Applying foliar aplication of urea solution and bitter salt in growing table potatoes
ZAJÍC, David
The thesis is focused on the application of foliar fertilizers in the cultivation of potatoes. The evaluation was based on one-year small-plot trial plot, which manages Selekta Pacov. An attempt was based on a comparison of revenue, market share tuber size and number of tubers in a bunch of potatoes, which were fertilized solutions bitter mangnesium sulfate and urea. The results obtained in 2015 largely reflected the drought which occurred during potato vegetation. Of backed results showed that foliar fertilization influences crop, size of table potatoes and starch content.
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Vliv hnojení močovinou a UreaStabil na výnos a kvalitu brambor
Pospíšilová, Markéta
The aim of my diploma thesis was to evaluate the effect of different doses of urea and UreaStabil fertilization on yield, tuber size and quality of potato tubers. A field experiment was carried out at the research station Valečov (Valkom Ltd.), the branch of the Potato Research Institute in Havlíčkův Brod during the years 2013 and 2014. Seven variants of fertilization were monitored in four repetitions of various doses of nitrogen fertilizer application as urea and UreaStabil and were compared to the unfertilized check variant. Two potato varieties, each with different lenght of the growing season, an early Karin and mid-early Red Anna, were used for comparison.
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Uplatnění močoviny s inhibitorem ureázy při hnojení brambor
Musilová, Ludmila
The focus of the dissertation thesis called "The use of urea with urease inhibitor in the fertilisation of potatoes" was on the effect of nitrogenous fertilisation with urea and urea with urease inhibitor NBPT (urea stabil) on changes in the content of Nmin in the soil and in nutrients available post-harvest, tuber yields, starch content, macro-elements N, P, K, Ca, Mg and Cd in tubers and tops, content of essential and non-essential amino acids in tubers. To complete the experiment we established a pot trial without the plant where we monitored in regular intervals for 24 days the effect of urea, urea with urease inhibitor (urea stabil) and ammonium nitrate with limestone on changes of the content of Nmin and on the individual forms of nitrogen in the soil. The field trial was conducted at the School Farm in Žabčice (south Moravia; maize-growing region) with potato varieties Karin and Red Anna. The trial involved 7 fertilisation treatments: 1) 100 % urea; 2) 80 % urea; 3) 60 % urea; 4) 100 % urea stabil; 5) 80 % urea stabil; 6) 60 % urea stabil; 7) unfertilised mineral treatment (control). A pot trial was established in the laboratory of Mendel University in Brno using 4 treatments of fertilisation: 1) unfertilised (control); 2) ammonium nitrate with limestone - LAV; 3) urea; 4) urea stabil.
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Dietary supplements in sport nutrition and possibilities of monitoring their effect on efficiency of sportsman.
Vlčková, Lenka ; Hrstka, Miroslav (referee) ; Vránová, Dana (advisor)
This work deals with dietary supplements intake and its impact on the performance rise and muscle mass growth of bodybuilders. Twenty-five students (bodybuilders) were chosen for the purpose of this survey; they were divided into four groups according to the dosage of creatine and other dietary supplements (proteins and maltodextrine). Changes of the measured parameters (weight; bustline; weistline; limb girth; body lipid content; creatine and urea content in the urine) were compared during the survey. The tested dietary supplements impact on the muscle mass growth was evaluated on the basis of the acquired results.
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Technology of Selective Catalytic Reduction
Kudlík, Michael ; Janoušek, Michal (referee) ; Dundálek, Radim (advisor)
This bachelor thesis is focused on technology of selective catalytic reduction. The technology was created as a result of introduction of strict emission limits which manufacturers with this technology reach. First part of this thesis deal with the composition of the exhaust gases, where are dismembered the various elements, and then are given here the European emission limits.Then is thesis focused on ways of reducing emissions of diesel engines which describes the function of exhaust gas recirculation and a function of selective catalytic reduction. Then are described development systems selective catalytic reduction. The conclusion consists of the use and application of Automobile Manufacturers.
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Emission Levels for Tractor Engines
Vaško, Roman ; Beran, Martin (referee) ; Dundálek, Radim (advisor)
This bachelor’s thesis deals with the emission standards of tractor engines. There is a tightening of standards in recent year generally. The former part of the thesis contains description of specific pollutants that are exhaled into the air. The second part is focused on newly presented Tier 4i standards (Stage IV). The third part includes technologies, which provide engines to meet new regulations. The last chapters deal with an overview of the largest manufacturers of tractors, including Zetor, and their approach to emissions.
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Reaction of selected potato varieties to foliar application of urea and bitter salt
HENŽEL, Vít
The effects of foliage nutrition facilitate efficient and immediate supply of nutrient needs during the vegetation period. The objective of this thesis is to assess the effects of foliage application of magnesium sulfate and urea for potatoes, that are intended for processing into starch production. There were two sorts of potatoes with different vegetation period under study, an early variety called as Bernard and semi-late variety called as Ornella. The foliage fertilizers were applied in three different variants. In the first variant the sorts of potatoes were fertilized by solution of magnesium sulfate, in the second variant by solution of urea and in the third variant the solution of magnesium sulfate and urea was used. The assessment of the experiment consists of yield on tubers, contain of the starch, yield on starch, the average amount of tubers per tuft, the average weight of tubers and profitability of used foliage fertilizers. In the year under the study the increased yield on tubers and on starch was detected in all three used variants. Both sorts of potatoes showed the best results when the solution of bitter salt and urea was applied. The increased yield on starch was reached through the increased yield on tubers preserving the high contain of starch. The yield of starch increased by 8 % in the semi-late variety of potatoes Ornella. The early variety of potato Bernard showed the increase of yield on starch by 14%.
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Vliv jízdního režimu nákladního vozidla na spotřebu vodného roztoku močoviny v systému selektivní katalytické redukce
Marek, Vít
This thesis titled Impact of driving mode on urea consumption describes the influence between different drive modes and conditions of urea injection into the selective catalytic reduction system. The first half of the thesis deals with the theoretical part. Introduction deals with the theory of formation of harmful pollution in exhaust emissions, the effects of pollutants and legislative regulations. There are also explained key parts of different systems, reduction of harmful exhaust gas components . The main emphasis is put on the selective catalytic reduction. Other chapters are devoted to the methodology and testing procedures . The aim of this thesis is a presentation of the system regulation, its pros and cons under various operational modes.
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