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The invasion apparatus of protozoan parasites
Novotná, Veronika ; Petrů, Markéta (advisor) ; Štáfková, Jitka (referee)
Protozoa parasitism has evolved many times independently in the history of eukaryotes. Some organelles were reduced, and specific invasion apparatuses were developed by adapting to the host. These apparatuses help to penetrate host cells and escape the immune system. The thesis summarizes the findings of the invasion apparatus and invasion of four selected unicellular protozoan parasites of genus Plasmodium, Microsporidia, Ichthyobodo and Rozella. Each chapter includes general knowledge of the parasite, its structure and life cycle. Keywords: Apicomplexa, Plasmodium, Microsporidia, Ichthyobodo, Rozella, merozoite, glideosom, moving junction, polar tube, germination, cytostome, zoospore, germ tube;
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Development of dual technology for the treatment of winter rape and spring barley seeds
OLŠAN, Pavel
The dissertation thesis deals with the use of low-temperature plasma and biological treatment of winter rape seed and spring barley as an alternative to chemical dressing in relation to the Decree on Integrated Pest Management 205/2012 Coll. The seed treatment technology is conceived as a two-phase process, where a lowtemperature plasma discharge of the Gliding Arc type ignited in the air at atmospheric pressure is used in the first step and a biological preparation containing spores of Trichoderma virens is applied to the treated seed as the second step. The plasma discharge treatment allows to increase the wettability of the seed surface, which improves the adhesion of the biological preparation in the second treatment step. Plasma also enables to activate selected processes taking place in seeds, which support their germination and subsequent growth. The content of the dissertation is divided into four sub-units which are mutually related: 1. determination of plasma discharge parameters for subsequent applications in the proposed technology, 2. carrying out laboratory experiments to assess the effect of plasma on seed, 3. carrying out field experiments to compare crop yields for different seed treatment options, 4. proposal for a regulation for seed treatment in a continuous mode by plasma discharge followed by application of the preparation and production of a functional sample. The work is markedly interdisciplinary and intervenes in the field of plasma physics, chemistry, plant production and construction of technical equipment. From the results achieved we can give a short summary: 1. Plasma discharge treatment of winter rape and barley seeds results in increased surface wettability. 2. No difference in the absorption of plasma treated and untreated seeds was observed during the experiments. 3. Developed dual seed treatment technology has the potential to increase yield. The dissertation thesis was realized within the project TA04021252 ? "Development of equipment for physical treatment of seeds and malt using low-temperature plasma."
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Ecology of critically endangered \kur{Sedum villosum}-experimental study
DILLINGEROVÁ, Hana
The aim of this diploma thesis was to elaborate a literature survey and especially to conduct an experimental study of ecological demands of the critically endangered species Sedum villosum. The localities, on which S. villosum formely occurred, disappeared largely because of habitat destruction by draining and plowing of wet meadows, or by the abandonment followed by development of high vegetation and biomass accumulation. Therefore, this work could contribute to the knowledge of shadow influence and surrounding vegetation concurency on seed germination and flowering of this species. Retrieved knowledge can be later used for rescue program realization (repatriation options, strengthening populations, habitat requirements). The experiments showed a significant negative effect of the shading and the presence of surrounding vegetation (sedges, brown mosses and peat mosses) on germination and flowering of S. villosum. Furthermore, it was found that S. villosum is able to germinate directly on water surface, with seed germination even higher than on wet soil. The experimental sowing, planting and subsequent monitoring of germination, growth and survival of S. villosum at the rehabilitated area of Knížecí Pláně (NP Šumava) showed that it is possible to strengthen S. villosum population by sowing and planting autochthonous material on prepared areas with a torn down turf. As an acceptable tools seems to be using mulch from mown in the area of the original population. Similar process can be used in the future for restoring historical localities of this species.
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Evaluation of modifications in content of selected bioactive substances in flax (\kur{Linum usitatissimum} L.) seeds during germination
HAŠKOVÁ, Kateřina
This thesis focus on analysing effect of germination on some groups of bioactive compounds in flaxseed. We used flaxseed variety named Libra. Its germination was 10 days long, done in two different conditions: in the dark and fully iluminated for 24 hours daily. Samples were taken on 2nd, 4th, 6th, 8th and 10th day. Following analyses were performed: determination of dry matter content, lipid content, content of nitrogenous compounds, content of proteins and protein spectrum, chlorophyll content, content of total polyphenolic compounds and antioxidant activity. Content of dry mater was proved to decrease during germination as well as lipid content regardless of light conditions. Amount of nitrogenous compounds stayed relatively unchanged during germination. Whereas amount of proteins increased depending on duration of germination and light conditions. Hydrolysis of proteins during germination was proved by protein spectrum analysis. Content of chlorophyll inreased in light-germinated seeds only. Increasing effect of germination on total polyphenolic compounds content and antioxidant activity was proven as well. Moreover aproximately two-fold rise of values was reported after defatting of samples.
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Mechanisms that control physiological seed dormancy
Řezková, Natálie ; Ponert, Jan (advisor) ; Vosolsobě, Stanislav (referee)
Physiological dormancy is an important developmental trait ensuring that seed does not germinate when the environmental factors are appropriate only temporary. The transition from seed dormancy to germination is regulated by a large number of factors and the phytohormone abscisic acid (ABA) plays a crucial role. Enhanced response to ABA and its biosynthesis is a key mechanism in dormancy induction and maintenance. ABA interacts antagonistically with gibberellins (GAs). Therefore GA biosynthesis and ABA catabolism are positive germination regulators. However, other phytohormones are also involved in the regulation of dormancy and germination. The most studied is ethylene which supports germination similarly to GA. Numerous factors affect dormancy at molecular level, namely chromatin remodeling, gene products that function only in dormancy regulation [e.g. DELAY OF GERMINATION 1 (DOG1)] or gene products that mediate seed response to environmental factors. The dormancy, its induction, depth and release, is driven not only by environmental conditions affecting mature seeds, but also by conditions acting during seed maturation in a maternal plant when the primary dormancy is induced. Requirements for dormancy release and germination induction may vary considerably between species. The physiological...
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Effect of climate on germination and selection of different genotypes
Duongová, Thuy Lien ; Münzbergová, Zuzana (advisor) ; Dostál, Petr (referee)
Understanding the response of species to climate change and their ability to adapt is the key to describe the future development of plant communities. The aim of the study is to determinate intraspecific variability in germination of Festuca rubra from different original climates in response to novel climatic regimes. This study also observes if different climatic regimes lead to selection of different genotypes. Festuca rubra is a widespread clonal grass occurring in the Northern hemisphere. The plant material comes from 11 localities distributed along a climatic grid of factorially crossed temperature and precipitation situated in western Norway. The project was carried out in growth chambers, where the germination of seeds was monitored in two different temperature conditions and in two moisture treatments. Germinated seeds were planted into pots remaining in the same treatment where they germinated. Seedlings from one Petri dish grew together in one pot. One population, from the coldest and the driest original locality, growing in the warm-wet and cold-wet treatments was genetically analysed using microsatellites. Germination of the species was higher and faster in warm than in cold conditions, showing that germination of the species is enhanced by higher temperature. Germination was higher in...
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Vitality and germination juniper (\kur{Juniperus communis}) seeds
OKROUHLÁ, Ilona
The thesis focuses on the fertility and vitality of common juniper seeds (Juniperus communis). Random galbula samples from two areas were tested. Amount of seeds per one galbula were compared, seed fullness and vitality were compared via the colouring method, as well as the weight, as well as a try to cancel the dormancy using gibberelinic acid GA,, following the aim to plant more seedlings. The results were compared to seed values and to findings from other authors. If the issue of lower seed fullness and vitality is clarified, more suitable methods of juniper population proliferation and support maybe worked out.
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Structure and function of the invasion apparatus of microsporidia
Dohnálek, Vít ; Doležal, Pavel (advisor) ; Vávra, Jiří (referee)
Microsporidia are obligate intracellular parasites that cause significant economic damage and can infect humans. They had evolved highly specialized invasion apparatus that is unique to them. During the invasion the sporoplasm is released from the spore wall and it is transferred to the host cytoplasm through the polar tube. Current research on the invasion apparatus has been focused mainly on the polar tube that is its most prominent structure. Polaroplast and posterior vacuole remain out of the main interest, although they are necessary for the activation and execution of the invasion. If the right combination of environmental factors occurs, the organization of the polaroplast begins to change, the posterior vacuole starts to swell and the polar tube is discharged. Sporoplasm is eventually pushed through the tube into the host cell by growing posterior vacuole. The mechanism has not been explained yet however plenty of theories are trying to explain the germination. This work summarizes current theories and knowledge of structure and function of particular parts of the invasion apparatus. Key words: Microsporidia, invasion, polar tube, intracellular parasitism, germination
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Study of germination and emergence of industial hemp seeds
ŘEZÁČOVÁ, Veronika
Nowadays hemp is listed among plants with a wide range of potentials. It can be used as a source of fibre, oil, food or medicine. The aim of this work was to investigate optimal conditions for hemp seed germination grown in laboratory as well as field conditions and to propose the application of acquired findings. Hemp seed was split into 2 size groups: above 3 mm and 2 - 3 mm. Divided seeds with the humidity level of 6, 9 and 12% were stored at the temperature of 0, 8 and 20? 1°C for half a year. In 2 months interval, there were evaluated: germination, energy of sprouting, speed of sprouting, further seed viability and laboratory emergence. From next influences were evaluated the influence of dryness, wetting, autotoxicity, exudates of perennial weeds, seed treatment with the infusion of field horsetail and the grown stimulator. The best germination and laboratory emergence had seeds stored at 0°C. Test of seed viability confirmed significant decline in live seeds stored over 4 months at the 0°C temperature. The best germination was achieved by seed size 2 - 3 mm. Hemp requires sufficient amount of water for germination. Leachates from rhizomes of couch grass inhibited germination of hemp by 10%. On the other hand, germination was significantly increased by the stimulator.
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Agrotechnická opatření při pěstování a sklizni Stévie sladké (Stevia Rebaudiana Bertoni) v polních podmínkách
HAUSEROVÁ, Tereza
Stevia or Stevia rebaudina Bertoni is a plant with very intresting but up to this date not very utilised potential. It a subtropical plant with a sweeting power us much as 300 times higher than in case of sucrose. It is often used as a compensation for sugar- sweetener, suitable for diabetics and people who suffer from overweight. There is processed the characteristics of the plant, botanical description, conditions of cultivation and chemical composition. The practical part elaborates on growing stevia, her germination, reproduction, growing in field conditions and hibernation. The purpose of the thesis was to expand the knowledge regarding cultivation, reproduction methods and wintering options of the stevia plants. A field and a greenhouse experiments were staged with a focus on temperature indicators during the growing season and their impact on the total number of wintered plants. The dry matter yield of the plants was rated per an area unit and statistical processing with experiment results.
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