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Structure- and sequence-based identification of functionally important amino acids in a protein family
Peclinovská, Iveta ; Novotný, Marian (advisor) ; Pleskot, Roman (referee)
A group of small GTPases consist of over twenty protein families in the super class P-loop. It has a very diverse cell functions. Small GTPases regulate the formation of vesicular follicles, cytoskeleton and nuclear transport. They participate also on cell proliferation and signaling. The aim of my work is to find important amino acids that define family and distinguish each other. I focus on families Arf, Rab, Ran, Ras and Rho. At the Rho family I am also devoted to classes Rho, Rac and Cdc42. Amino acids are identified using bioinformatic programs selected Consurf and Sca5. The objective is also to test P2RANK specialized tool developed at the Charles University in Prague that predict ligand binding sites from protein structure in different families. Founding amino acids can have a big role in the functional divergence of individual families and classes of small GTPases and can be the basis for future study example for the proliferation of cancerous cells. 1.1 Keywords Powered by TCPDF (www.tcpdf.org)
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Application of Diffusion Tensor Imaging to Brain Gray and White Marker
Rulseh, Aaron Michael ; Vymazal, Josef (advisor) ; Hluštík, Petr (referee) ; Hájek, Milan (referee)
Application of Diffusion Tensor Imaging to Brain Gray and White Ma er A In the present work we explore the gray and white ma er applicability of diffusion tensor imaging (DTI). To evaluate effect of ferritin-bound iron on gray ma er contrast in DTI, we created an in vitro model consisting of agarose gel phantoms doped with ferritin, and validated our results in vivo on healthy volunteer subjects - years of age in the basal ganglia. We further explored the application of DTI to amyotrophic lateral sclerosis (ALS) and multiple system atrophy (MSA); neurodegenerative diseases with gray and white ma er pathophysiological components. In the ALS study, patients and age- and sex-matched controls were recruited, while the MSA study included probable MSA subjects ( MSA-P, MSA-C) and age- and sex-matched controls. We found that ferritin-bound iron may make a signi cant contribution to DTI scalars in gray ma er regions of the brain, mediated by eigenvalue repulsion. is has important implications for DTI studies targeting gray ma er regions, especially in adolescence and in diseases associated with altered brain-iron load. In ALS, we found altered diffusion in the corona radiata and callosal body, and changes in R in the caudate nucleus and frontal white ma er. In MSA, we observed widespread white ma er changes associated...
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Production of flanges using the procedure PPAP , and ISO 9001
Prokeš, Tomáš ; Zahradníček, Radim (referee) ; Mouralová, Kateřina (advisor)
In the first part of this thesis is described implementation of quality management system in a small company according to ISO 9001. Next part is about production process by method PPAP. In this section is solved primarily ISO 9001 standard, statistical control of production and certification company according to requirements of ISO 9001. Second part is focused on mass production of flange by using the company facilities Kovobrábění Sobotka. In the end of thesis are reflected weaknesses of production. In the conclusion is described technical and economic evaluation of implementation QMS and machining sample parts.
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Assessment of the process stability
Kostečka, Petr ; Bednář, Josef (referee) ; Kotek, Luboš (advisor)
This master`s thesis is focused on the application of statistic process control in engineering production and it is focused on comparison with method Beta correction. The aim of the diploma thesis is to find suitable regulation of the producing process with respect to provision of sufficiently high level of quality of products at the process of achievement of adequate costs conneced with process monitoring. The basic principles of both methods are solved in the theoretical part of this diploma thesis. Both methods are compared in particular producing process in the practical part of the thesis. Conclusions and suggestions are done on the base of gained data.
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Repeatable Torque Load Equipment Design for Vehicle Steering System Testing
Novák, Pavel ; Ludvík, Jan (referee) ; Ramík, Pavel (advisor)
The object of this work is design braking device with high repeatability and reproducibility that is used to analyse the measurement system. I developed this work in cooperation with TRW-DAS Dačice a.s. At the beginning, I made a search of the chassis system, testing of ball joints and steering. Furthermore, I made search of the MSA and the types of braking devices. The rest of the work is focused on the design and the thermal analysis of braking device. Then there are the actual test.
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Analysis of process for production of small plates
Talanda, Jiří ; Fiala, Alois (referee) ; Koška, Petr (advisor)
Theme of the Master’s thesis is analysis of process for production of small plates. Main parts of the thesis are analysis of the production process, process mapping, data capture, analysis of the data by Pareto analysis and find causes of nonconformities. Causes analysis is based on data analysis and process observation. It is divided to chapters by factors which are influencing the process. Optimization measures are designed with regard to their simplicity of realization and system in organization.
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Optimization and use of standardized manufacturing routines for the Common Rail Flange production of high pressure pumps.
Doležal, Jan ; Prokop, Jaroslav (referee) ; Píška, Miroslav (advisor)
Objective of the Project of optimization and introduction of standardized procedures in flange production is to plan, determine and introduce optimal procedures based on time scan and MTM analysis; to determine standards, to establish standardized procedures for multi -machine operation and visual control and to train personnel according to new procedure. Project is expected to stabilize process, decrease work in process production, improve quality and increase volume of flange production to 2200 pieces per day for one production line. Furthermore to provide comparison of standardization influence on operations in flange production process, to perform quantitative analysis of several working instructions on visual control workplaces by statistics method (R&R study).
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Measurement System Analysis for Attributes
Širjovová, Zuzana ; Martišek, Karel (referee) ; Bednář, Josef (advisor)
Method Measurement system analysis or MSA is very exploited method for determination of the number of same and different produces today. Assesment of rightness of measurement system is often used but on the other hand it is mathematically deficiently catched up. The measurement systems analysis is more necessary, if a production system and the produce are very complex and advanced. Therefore requierement of MSA use in different types of industry is very common. MSA for attributes is the basic subject of my bachelor thesis. Complete MSA for attributes will be shown here with collecting datas and then their processings and evaluations. Software MINITAB 15 will be used for this procedure. The bachelor’s thesis was supported by project from MSTM of the Czech Republic no. 1M06047 Center for Quality and Reliability of Production.
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