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Importance of capillary electrophoresis in Human Genome Project
Klepárník, Karel
The fundamental contribution of capillary electrophoresis to the HumanGenome Project can be demonstrated on the massive application of multicapillary fully automated instruments for DNA sequencing. Sequencing is the most successful application of capillary electrophoresis from commercial point of view. Also in the filed of the research of genome DNA polymorphism plays capillary electrophoresis an important role.
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Podmínky pro vysokou citlivost detekce a separační účinnost při separacích klastrových aniontů boru
Horáková, Hana ; Grüner, Bohumír ; Vespalec, Radim
The knowledge of UV-VIS spectra of investigated compounds is neccessary for the optimisation of detection sensitivity. The symmetry and narrow width of migrating zones require substantially improved dissolving power of the sample solvent and background electrolyte. Sodium borate buffer of pH 9.5 is more proper for separations of boron cluster anions in uncouted capillaries then sodium phosphate buffer of pH of 7. Decrease of mobilities of boron cluster anions with time has been found in chiral separations with .beta-CD if acetonitrile was present in background electrolyte
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