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Cannabinoids and Time Perception
Popova, Anastasia ; Nekovářová, Tereza (advisor) ; Honc, Ondřej (referee)
Interval timing (i.e.the perception of time in the seconds-to-minutes range) allows various animal species including humans to estimate duration of elapsed time. This process can be disrupted by cannabinoids and most often it is subjectively perceived as time deceleration. This work will describe the main models of interval timing, neuroanatomical structures involved in this process, and the main neurotransmitters. The results of studies examining the effect of cannabinoid on the interval timing, possible explanations according to the particular models, and the interaction between the cannabinoid and dopaminergic systems will be described as well. Keywords: interval timing, time perception, cannabinoids, dopamine
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Preparation of anti-dandruff cosmetic products using technical hemp
Posoldová, Kateřina ; Fialová, Lenka (referee) ; Němcová, Andrea (advisor)
The main theme of this bachelor’s thesis is technical hemp and its effects on dandruff. Hemp has been a plant known for years not only because of the hallucinogenic substances which it contains, but also those for that are beneficial to our bodies. The theoretical part characterises hemp and the active substances from it. Then the thesis discusses about skin and a condition called dandruff. The next part describes yeast, which is very often occurs in hair skin disease. The practical part involves preparing ethanol extracts from two varieties of hemp, determining the active substances in the prepared extract and cosmetic products with added hemp extract. Extracts and products were tested using the antimicrobial method. The inhibitory effect was studied on representatives of bacteria and yeast.
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Using of cannabis extracts for the acne preparation of cosmetic products
Pelánová, Lenka ; Langová, Denisa (referee) ; Němcová, Andrea (advisor)
Hemp belongs among important plants with therapeutic effects thanks to its high content of substances. This bachelor thesis brings information about the use of hemp in acne preparations. The theoretical part of this thesis focuses on the characterization of hemp and its active substances. The next chapters deal with acne and Propionibacterium acnes, which is involved in the course. For the experimental part, the extracts of two hemp varieties (Ferimon and Carmagnola) were characterized in terms of antioxidant and cannabinoid presence. The cosmetic preparations with hemp extract content were prepared and commercial products were purchased too. In both the extracts and the cosmetic preparations, their inhibitory effect against Propionibacterium acnes was observed. Micrococcus luteus, Candida glabrata and Escherichia coli were also tested. The results show that dried hemp flower extracts contain the largest amount of active substances. All hemp extracts had effect against gram-positive bacteria, in cosmetic preparations it was minimal effect or no effect.
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Food and cosmetic use of technical hemp
Puškárová, Radka ; Skoumalová, Petra (referee) ; Němcová, Andrea (advisor)
The thesis occupies with the use of hemp in cosmetics and in food processing. In the theoretical part hemp was characterized by the content of active substances. In the experimental part the thesis is concerned with characterization of two different hemp varieties Carmagnola and Ferimon in terms of presence of active substances. The next part focuses on preparation of cosmetic with hemp content. In cosmetic preparations and in the extracts the inhibitory effect against Propionibacterium acnes, Micrococcus luteus, Escherichia coli and Candida glabrata was tested. The content of fatty acids and vitamin E was determined in hemp seed. The results show that dried hemp flower extract contains the largest amount of active substances and has the greatest inhibitory effect against all tested bacteria except Candida there was no effect. Cosmetic preparations did not show almost any inhibitory effect because of low concentration of active substances. The hemp seed contained high amount of PUFA and essential fatty acids. Vitamin E was found out in all of three hemp oils.
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Active components of technical cannabis and their application in cosmetics
Kubáčková, Eliška ; Bokrová, Jitka (referee) ; Němcová, Andrea (advisor)
Hemp is unique plant with many therapeutic effects thanks to its large content of substances. This bachelor thesis brings information about characterization of main biological active substances in cannabis sativa, which are described in theoretical part of this thesis and determined in experimental part of this thesis. As a main active substance were determined antioxidants and cannabinoids for which the liquid chromatography method was optimized. This bachelor thesis also describes the antimicrobial effect of active cannabis substances for which it is used in the cosmetic industry. This antimicrobial activity has been studied in terms of the effect against dandruff, which is primarily caused by the high incidence of Malassezia yeasts on the skin of human hair. For the experimental part, the extracts of the technical hemp and more specific varieties of Bialobrzeskie and Finola were tested, which were further used for the preparation of the anti-dandruff shampoo. By analyzing the active substances in hemp extracts, the uniform result was obtained - the highest content of antioxidants and cannabinoids was reached by preparing of extract from dried hemp flowers. In the last part of the thesis, the antimicrobial effect of hemp extracts and prepared hemp shampoos was determined on selected strains of microorganisms and it was observed antimicrobial activity in extracts and shampoos againts Malassezia furfur yeast.
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Use of technical hemp in acne preparations
Žáčková, Kristýna ; Kovalčík, Adriána (referee) ; Němcová, Andrea (advisor)
Hemp belongs among important plants with a long industrial tradition. However, its first use was originally for healing. In medicine, it is mainly applied thanks to the content of many biologically active substances, such as cannabinoids. This thesis is concerned with the use of hemp in acne preparations. The theory focuses mainly on the characterization of hemp and its active substances. The other chapters deals with cosmetic preparations and acne itself, and Propionibacterium acnes as well, which is involved. Within the experimental part, the extracts of two hemp varieties (Finola and Bialobrzeskie) were characterized in terms of antioxidant and cannabinoid presence. The cosmetic preparations with hemp extract content were prepared including a complete cleansing set for acne skin, concurrently commercial products of this sort were purchased. In cosmetic preparations and the extracts their inhibitory effect against Propionibacterium acnes was studied as well. Also Micrococcus luteus, Escherichia coli and Candida glabrata were tested. The results show that dried hemp flower extracts contain the greatest amount of active substances, so they have a higher antioxidant and antimicrobial effect. The presence of individual cannabinoids differed mainly depending on the variety. All hemp extracts had effect against gram-positive bacteria, in cosmetic preparations it was lower. Nevertheless hemp antibacterial effect has been proven.
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A study of signaling and cytoprotective potential of cannabinoid GPR55 receptors in PC12 cells
Pavluch, Vojtěch ; Novotný, Jiří (advisor) ; Ostašov, Pavel (referee)
At the end of the 20th century it was known that cannabinoid drugs interact with two receptors, CB1 and CB2. Subsequent pharmacological studies have confirmed that there are other receptors interacting with cannabinoids. GPR55 is a transmembrane G protein coupled receptor, which together with the receptor GPR18 and GPR119 belong to a group of new cannabinoid receptors and is involved in the function of the endocannabinoid system. In addition to some of cannabinoid substances, it is stimulated primarily phospholipid lysofosfatidylinositolem. LPI-dependent signaling GPR55 plays an important role in the regulation of many physiological and pathological processes, such as pain, inflammation, cell proliferation, or endothelial function. It was found that LPI confers tolerance to ischemic brain damage and has a cytoprotective effect on the pyramidal cells. The aim of the study was to determine whether the application of five ligands induce phosphorylation of protein kinase ERK 1/2, Akt and activate the GTPase RhoA and whether activation of the receptor GPR55 has cytoprotective effect in model cell line PC12, in which hypoxic conditions were simulated by adding CoCl2. For working methods were used SDS-PAGE, Western bloting and colorimetric measurement. Pharmacological studies in recent years have shown...
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Isolation of compounds from Cannabis sativa and their biological effect
Baranová, Zuzana ; Vespalcová, Milena (referee) ; Šmejkal,, Karel (advisor)
The object of this thesis is a traditional and also controversial plant Cannabis sativa. The theoretical part is focused on botanical side of this plant and its presently used medical forms. All previously isolated substances and their observed biological activity are described in detail. The experimental part of this thesis describes the isolation and identification of substances of the chloroform extract fraction obtained from this plant. Chromatographic methods were used for isolation – analytical and semipreparative HPLC. Obtained substances were identified using UV and IR spectrophotometry. Then the biological effect of obtained substances was observed on chosen microorganisms.
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The technology of cultivation and fertilization hemp (Cannabis) and ways of utilization
JAROŠOVÁ, Markéta
Contemporary is Hemp (Cannabis sativa L.) considered as a very promising plant whose parts can be used almost completely. Hemp is grown especially for fiber and seed. The plant is good biodegradable, so it causes no problems with waste disposal. The root system is deep and loosens the soil and prevents erosion. Hemp accumulate heavy metals from contaminated soils.To achieve the economic benefit are suitable fertile , deep soils with good nutrient supply and neutral or slightly alkaline reaction. Average seed yield in the Czech Republic is 0.6 t ha-1 and 6-7 t ha-1 stem. This study focuses on summarizing information about the cultivation, fertilization and the possibilities of using hemp.The first part is devoted to the origin, botanical characteristics, morphology and anatomy and principles of cultivation. The second part deals with the chemical composition, effective substances and use of cannabis in various industries. In this work was proposed five years crop rotation period for a variety Bialobrzeskie, grown for seed and stem. Than were selected pre-crops suitable for business without animal production and determined doses of nitrogen, phosphorous, potassium and magnesium fertilizers and suitable harvest mechanization.
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Abuse of illegal drugs by student of University of South Bohemia
PLACHÁ, Markéta
The thesis research the frequency of illegal drugs use by student sof Univerzity of South Bohemia in the Czech Budějovice. In the theoretical part are characterized by drugs, illegal drugs and the effects of drug use on health, describes the emergence of drug addiction and its types and developmental stages of drug use. Furthermore, illegal drugs are divided into categories according to their effects and further described. The main aim of the practical part is the analysis of illegal drug use by students of the University of South Bohemia. On the basis of a questionnaire compiled data was found and it was statistically analyzed by the method, compares and conclusions were drawn. Over the past year have used an illegal drug 37.83% of respondents, that 2.91% of the respondents use illegal drugs daily. Earlier experience with illegal drugs has 18.78% of students of the University of South Bohemia. Past experience with drugs is more common in women, while use in the last year is much more common in men. The most commonly used substances are cannabinoids, as well as hallucinogens, opiates and finally stimulanci.
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