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National Repository of Grey Literature

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	Physical principles of transmission and scanning electron microscopy BÍLÝ, Tomáš This bachelor thesis focuses on the description of transmission and scanning electron microscopy. It describes physical principles and defects arising during image formation in electron microscopes as well as the methodology of adjustment. The basic parts of electron microscopes are looked into more closely herein with consideration to the lectures. Practical implementation is demonstrated on following microscopes JEOL: JEM-1010, JSM-7401F, JDM-6300. Detailed record
	Collection solid angle of backscattered electrons in environmental scanning electron microscopy Wandrol, Petr This paper deals with an influence of the collection solid angle on a magnitude of detected signal. The presented results of measurement were gained by an observation of the specimen of carbon covered with a thin layer of gold. The collection solid angle was changed by application of aluminium masks mounted on the scintillator. As a working environment in the specimen chamber air and saturated water steam were used. Detailed record
	Observation of biological material in low voltage transmission electron microscope Vancová, Marie ; Nebesářová, Jana Different methods of specimen preparation for low voltage transmission electron microscopy (LVEM) were evaluated. The contrast of the image in LVEM, contrary to high voltage TEM, is strongly influenced by the thickness of the specimen. The resin embedding media used and the process of sectioning employed contributed to image quality and resolution. Detailed record
	Comparison of the cell structure of tissues prepared by different methods for transmission electron microscopy Vancová, Marie ; Nebesářová, Jana The aim of this work is to show how methods which are able to retain antigenicity of tissues can influence the structural appearance of the specimen. We compared the ultrastructure of tissues conventionally embedded in hydrophobic epoxides, with tissues embedded in hydrophilic acrylic resins (polymerized by heat or UV light) and cryo-sections. Detailed record
	The first experience with the specimen preparation for low-voltage transmission electron microscope Nebesářová, Jana ; Vancová, Marie The low-voltage transmission electron microscope, working in the TEM mode, requires ultrathin sections with a thickness 20-30nm. This demand brings problems with a section cohesion caused by insufficient resin infiltration and with a chatter caused by unequal thickness of the section. The contrast of unstained biological specimens in LV TEM is comparable with the contrast obtained in HV TEM of stained specimens. Detailed record
	Electron microscopical study of embryonic envelopes in the tapeworm Proteocephalus torulosus (Cestoda: Proteocephalidea) Bruňanská, Magdaléna ; Nebesářová, Jana ; Scholz, Tomáš The ultrastructure of the embryonic envelopes of the cestode Proteocephalus torulosus (Batsch, 1786), a parasite of cyprinid fish, was examined by transmission electron microscopy. Detailed record
	The importance of fixation procedures for transmission electron microscopy of haemocytes of Pyrrhocoris apterus Slavíčková, K. ; Berger, Josef ; Nebesářová, Jana ; Němec, Václav The importance of fixation procedures for transmission electron microscopy of haemocytes of Pyrrhocoris apterus. Detailed record
	Scanning Slow- and Auger Electron Miscroscopy Hrnčiřík, Petr Detailed record
	Electron Spectro-Microscopy with Multichannel Detection of the Signal Čižmár, Petr Detailed record
	Autoemisní katody pro elektronové mikroskopy Delong, Armin ; Kolařík, V. Field emission emitters have significantly improved the coherence of electron guns in electron microscopes and allowed to reach atomic-scale resolution in the case of crystal imaging. Even now, thirty years after the first application of field emission emitters, one can hardly say that the development of electron emitters has been finalized Detailed record

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