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Assessment of regional climate models performance in simulating present-day climate over the area of the Czech Republic
Crhová, Lenka ; Kalvová, Jaroslava (advisor) ; Kliegrová, Stanislava (referee)
Title: Assessment of regional climate models performance in simulating present-day climate over the area of the Czech Republic Author: Lenka Crhová Department: Department of Meteorology and Environment Protection Supervisor:doc. RNDr. Jaroslava Kalvová, CSc. Supervisor's e-mail address: Jaroslava.Kalvova@mff.cuni.cz Abstract: Today a great attention is turned to climate changes and their impacts. Since eighties the Regional Climate Models (RCMs) are developed for assessment of future climate at regional scales. But their outputs suffer from many uncertain- ties. Therefore, it is necessary to assess models ability to simulate observed climate characteristics and uncertainties in their outputs before they are applied in consecu- tive studies. In the first chapters of this thesis the sources of uncertainties in climate model outputs and selected methods of climate models performance evaluation are reviewed. Several methods of model performance assessment are then applied to si- mulations of the Czech regional climate model ALADIN-Climate/CZ and selected RCMs from the ENSEMBLES project for the reference period 1961-1990 in the area of the Czech Republic. The attention is paid especially to comparison of simulated and observed spatial and temporal variability of several climatic elements. Within this thesis the...
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Quality control of molecular biology methods in transplantation medicine
Kotrbatý, Jiří ; Kolesár, Libor (advisor) ; Schierová, Michaela (referee)
This work is focused on the description of quality control of molecular-biological methods used in transplant medicine, ie methods of HLA typing. Quality control is part of the quality management system. It consists of several components: validation, verification, internal quality control and external quality assessment. Each medical laboratory, including laboratories engaged in HLA typing, these elements must be applied to its routine operation. Validation is done before the introduction of methods and verifies whether the method is correct for the intended use. Verification is to verify that the validated method is used correctly in laboratory conditions. Internal quality control is based on an analysis of known positive and negative samples and evaluate the accuracy and precision of the whole analytical process. The external evaluation of the quality of the different laboratories analyze the same sample and the results are compared to each other. Key words: HLA typing, validation, verification, internal quality control, external quality assessment
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Enneagram-Development of Personality Type Test
Kilianová, Jana ; Bahbouh, Radvan (advisor) ; Niederlová, Markéta (referee)
The enneagram typology describes nine personality types and their mutual relations. It is a theoretical construct whose psychological validity is still being scientifically verified. The focus of this work is to develop a reliable and valid psychometric tool for recognizing the enneagram type and thereby verify the validity of this typology. The theoretical section describes the enneagram system on the basis of available literature and reviews research that set it in the psychological context. The empirical section deals with the development of the test to determine the ennea-type, with verification of its psychometric characteristics and criterion validity, and at the same time it examines the construct validity of enneagram typology. The criterion to review the validity is The Essential Enneagram Test by David Daniels, type identification by interview and self-identification. A quantitative data analysis was conducted on the sample of 300 persons of both, men and women of all ages using various kinds of statistical methods. The findings indicate that the tool developed to identify the ennea-type is reliable, of good psychometric quality and satisfactory criterion validity. Furthermore, the research supported the existence of nine enneagram types as per the theoretical construct, yet did not...
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Using of quantitative DNA method as a screening tool for effecient genotyping of samples in forensic DNA laboratory.
Koljenšič, Ivana ; Stenzl, Vlastimil (advisor) ; Daňková, Pavlína (referee)
Quantification of human DNA in forensic samples is an important step during STR profiling because the STR genotyping is sensitive to the quantity of DNA used in the PCR reaction. This study focuses on the importance of quantification in the entire process of genetic analysis. Two real time PCR platforms (Roche LightCycler480 System and ABI 7900 RT PCR) were used to compare two commercial kits in terms of DNA quantification. It was found out that accuracy of absolute quantification values in commercial quantification kits is strongly dependent on the construction of calibration curve. Especially low template DNA samples were used to assess whether QuantifilerTM or Plexor® HY System can determinate a minimum quantification value (cut off value) below which STR profiles would consistently fail to be detected. The usage of Plexor® HY System enabled to determine the cut off quantification value more exactly probably due to different molecular background and chemistry used in this kit. Reliability and other issues connected with cut off value are discussed. In order to better understand the relationship between the quantity of DNA and the number of detectable loci series the dilution experiment with standard DNA007 was done. Quantitative and qualitative consequences of input DNA amount in evaluation of...
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The verification of accredited analytical method for the determination of second-generation triglycerides using the biochemical analyzer Advia 1800
JANDOVÁ, Helena
Triglycerides belong to a large group of lipids. They are biologically very important substances for the human body. Chemically they are glycerols which are bound by ester bonds to fatty acids. Triglycerides are feeding, and that our organism has spend triglycerides must be broken down into glycerol and fatty acids, and then they are absorbed and transported to tissues. Determination of triglycerides in serum, respectively plasma, is particularly important for the prevention of atherosclerosis which is the leading cause of cardiovascular diseases, but also to diagnose and prevent such diseases as obesity or diabetes mellitus. To introduce method for the determination of the second-generation triglycerides via the biochemical analyzers Advia 1800 to be used in the ward of clinical biochemistry of Central Laboratories of Hospital Strakonice, a. s. there was need to carry out a verification of the method. This verification is subject ot the requiremets of ČSN EN ISO 15189 Medical laboratories Special requirements for quality and eligibility, according to which the Central Laboratory of Strakonice Hospital, a s. is accredited. It is further governed by the procedures for the validation/verfication methods, SOPO-C-06, drawn up by our laboratory. The results of the verification/validation are used for the evaluation of measurement uncertainties and to establish continuity and comparability of measurement results. On the basis of document mentioned above, the measurement of triglycerides in serum was conducted in two biochemical analyzers Advia 1800. These measurement led to the evaluation of the precision under repeatability conditions, precisions under conditions of reproductibility and accuracy. Precision under repetability condition was measured at twenty patients' samples in two concentration ranges. For evaluation of precisions under reproducibility conditions we used control materials validated in the EHK processes, exactly Liquid Assayed Multiqual 1 Lot 45651, the second check was with the help of Liquid Assayed Multiqual 2 Lot 45662 from the manufacturer BIO-RAD. These reference materials were measured every day in a singlet for twenty days. From the measured values the basic statistical parameters and analytical coefficient of variation were calculated. To evaluate the truthfulness they were also measured with the help of control materials from the manufacturer SEKK, s. r. o. Based on these measurements the basic characteristics were again calculated together with bias and total laboratory analytical error. Comparison of this error with a maximum mistake reccommended by the company SEKK, s. r. o. led to the evaluation of the method for the present purposes.
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Establishment of the method for determination of selected congeners of polychlorinated biphenyls in water using GC-ECD
TALAVÁŇOVÁ, Eliška
This bachelor thesis deals with the determination of selected congeners of polychlorinated biphenyls in water using GC-ECD. Firstly, the development and optimisation of the analytical method was performed. The following parameters of the analytical method were chosen for optimization: carrier gas flow rate, sample volume, injector temperature, splitless time, initial column temperature, temperature gradient and detector temperature. Then, extraction time of the liquid-liquid extraction was optimised. Finally, the method was successfully validated and applied to the analysis of a real tap water sample.
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Verification of basic parameters of complete blood count using analyser Sysmex XT 4000i, result comparison of patients' samples with analyser Sysmex XT 2000i in accredited laboratory.
MOUČKOVÁ, Lenka
Central Laboratories of Strakonice Hospital, joint stock company, are accredited according to CSN EN ISO 15189 Medical laboratories - Particular requirements of quality and competence. Requirements of verification and validation of methods emerge from this accreditation. Results of validation and verification processes are used to evaluate the uncertainty of measurement and assessment for continuity and comparability of measurement results. Introduction of a new type of automated hematology analyzer Sysmex XT-4000i in Central laboratories in Strakonice Hospital, joint stock company, in the hematology and blood storage section, requires verification and validation of basic examined blood count parameters on hematology analyzer Sysmex XT-4000i.The theme of the work is based on the requirements of CSN EN ISO 15189 and SOPO-C-06 Method validation/verification procedure for introducing laboratory hematology analyzer. Measurements of the basic parameters of blood count , namely leukocytes, erythrocytes, platelets, hemoglobin, hematocrit and mean corpuscular volume were done according to this document. Measurements of these parameters were used to evaluate a precision in terms of repeatability, precision in terms of reproducibility and accuracy. Parameters leukocytes, erythrocytes, platelets, hemoglobin, hematocrit and mean corpuscular volume were measured from twenty patient samples at two concentration levels to evaluate the precision in terms of repeatability. Basic parametres of blood count validated in EHK process were measured to evaluate the precision in terms of reproducibility. Reference materials e-check level 1 and e-check level 2 from Sysmex manufacturer were used as hematology control materials which moved within the physiological range. Measurements of these two reference materials were carried out daily during 20 days in a singlet. These measurements were used as the basis to calculate basic statistical parameters, the variational coefficient of repeatability and the variational coefficient of reproducibility from which variational analytical coefficient was calculated. The variational coefficient of reproducibility was used to compare methods of investigated parameters to the data specified by the manufacturer of the analyzer, as the manufacturer of the analyzer does not give the variational coefficient of repeatability. To estimate the validity, basic parametres of blood counts were measured to control materials from the company SEKK, Ltd.These materials are used to measure performance parametres. Validity measurements were used to calculate basic statistical parameters of which bias was calculated. It served to calculate the total analytical error of a laboratory and also it served to be compared to the total maximum error recommended by the company SEKK Ltd. in 2012. Passing-Bablock regression analysis was used to compare hematology analyzer Sysmex XT-2000i and hematology analyzer Sysmex XT-4000i, namely to compare the existing and the introducing analyzer and to compare open and closed mode of the introducing analyzer Sysmex XT-4000i. To compare analyzer Sysmex XT-4000i and Sysmex XT-2000i, which was regarded as referential, 40 patient samples were measured in a closed mode. To compare open and closed modes of analyzer Sysmex XT-4000i, 168 patient samples were measured. The measured values of all basic parameters were evaluated using MedCalc programme. The study showed that the device Sysmex XT-4000i meets the criteria required by CSN EN ISO 15189 Medical laboratories - Particular requirements for quality and competence and SOPO-C-06 Method validation/verification procedure for introducing laboratory hematology analyzer in Central laboratories Strakonice Hospital, Inc. section of hematology and blood storage. Comparison of the new hematology analyzer Sysmex XT-4000i to the current Sysmex XT-2000i showed that both analyzers are comparable.
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Vývoj, optimalizace a validace analytické metody na stanovení neurotoxinu \recke{beta}-N-methylamino-L-alaninu ve vodě pomocí LC/MS
HOŘEJŠÍ, Karel
This bachelor thesis deals with the development, optimization and validation of an analytical method for determination of neurotoxin \recke{beta}-N-methylamino-L-alanine in water using LC/MS. At first, the development and optimization of the analytical method was performed. For the optimization of the analytical method following parameters were selected: temperature of HESI and MS capillary, gases flow rate, voltage on S-lens, F-lens and capillary, collision energy. In addition, mobile phase composition, flow rate of mobile phase and injection volume of sample were optimized too. Then, the analytical method was successfully validated. Finally, the method was applied to the analysis of real samples of surface water.
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Data Backup and Data Storage
Uhlíř, Petr ; Svoboda, Tomáš (referee) ; Kříž, Jiří (advisor)
This thesis aims to clarify developments in backup, recovery and long-term storage of data on a general level and in the specific project NDK. Further notes on global trends in this field and discusses the idea of long-term storage, storage system and life cycles of this repository. Marginal manner the standardization used in this store.
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Proposal of company´s E-marketing
Hadrava, Martin ; Matějka, Jiří (referee) ; Dvořák, Jiří (advisor)
Thesis contains the basic theoretical resources for processing e-marketing of company; it offers two options of problems solving proposals. The first option contains basic web presentation with SEO optimalization. The second option, which is superstructure of the first one, is enriched by advertising activities on internet, which direct the user to the visiting of websites. These suggestions are consequently evaluated from the economical point of view.
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