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Influence of meadow management on the migration behavior of selected species of butterflies
Bubová, Terezie ; Langrová, Iva (advisor) ; Farkač, Jan (referee)
Migrations are the key process and one of the basic biological features, which allow survival of endangered species in fragmented landscape. In the last decades, agriculture intensification, industry and urbanization caused that number of natural butterfly habitats has significantly decreased. The localities originally suitable for butterflies are currnetly converted to farmland or building plots. This PhD thesis aimed to find a compromise solution of meadow management, which should allow a commercial use and concurrently do not jeopardize the continued existence of endangered or threatened species. The main idea of this study was in the intentional emigration causing in the middle of flight period, which should lead into resettlement of the population to another (potentially more suitable) locality. Possibility of aimed manipulation with butterfly emigrations would be considered as a major breakthrough in their protection.
The investigated species Phengaris nausithous (Bergsträsser, 1779) and Phengaris teleius (Bergsträsser, 1779) are both considered to be flagship species for protection of European butterflies at open meadows. This study is based on research, which was carried out in 2013-2015 at 17 patch in localities Dolní Labe, Czech Republic. In above mentioned years, one selected meadow was mowed in the middle of flight season and the emigrations were then monitored using mark-release-recapture method. Results of population and migration characteristics were calculated in MARK 8.1 software. Subsequently, the effect of mowing on emigration (comparison of emigrations from the meadow before and after mowing) was evaluated using Statistica 13.
The hypotheses of this study were based on the assumption, that the loss of resources after well-timed mowing should increase the activity of adult butterflies. During the monitoring, number of emigrations was increased only in flight season 2015. Unfortunately, the statistical evaluation did not show significant effect of mowing on migrations. On the other hand, the hypothesis dealing with colonization of nearest meadows was confirmed.
Effect of intentional emigration caused by management interventions was unfortunately not proved. However, we found out, that even though the population at one mowed meadow decreased clearly, the metapopulation size at the locality remained more or less stable.
At investigated (mowed) meadow, there was observed a sharp decline of P. nausithous abundance. On the other hand, P. teleius population was only slightly reduced. It is therefore conceivable, that P. teleius are not as susceptible to innapropriate term of mowing. The results obtained in our research conclusively displayed no possible positive effect of mowing in the middle of flight season and a necessity of suitable management maintenance at localities inhabited by Phengaris butterflies.
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Properties of Aerosol, Produced by Laser Ablation of Standard Materials for ICP-MS Analysis.
Holá, M. ; Nováková, H. ; Ondráček, Jakub ; Vojtíšek, M. ; Kanický, V.
Laser ablation (LA), together with inductively coupled plasma mass spectrometry (ICP-MS) as a detection system, has become a routine method for the direct analysis of various solid samples. The product of laser ablation contains a mixture of vapour, droplets and solid particles. All components are finally transported to a plasma by a carrier gas as a dry aerosol including mainly agglomerates of primary nanoparticles. In general, characterisation of aerosols by their particle size distribution (PSD) represents indispensable tool for fundamental studies of the interaction of laser radiation with various materials. The particle size distribution of dry aerosol originating from laser ablation of standard material was monitored by two aerosol spectrometers – Fast Mobility Particle Sizer (EEPS) and Scanning Mobility Particle Sizer (SMPS) simultaneously with laser ablation - ICP-MS analysis.\n
Fulltext: content.csg -  PDF
Plný tet: SKMBT_C22016102412241 - PDF
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The nursing process in a patient with chronic pain - usage of non-pharmacological techniques
Janůšová, Veronika ; Voleníková, Ivana (advisor) ; Horká, Eva (referee)
My thesis deals with chronic pain and how a nurse should handle it. This thesis consists of three parts. The first one focuses on pain in general, both on the history and the pain origin mechanisms. It also deals with pain classification. The main topic is chronic pain. Here I reflect basic characteristics of this pain, its influence on human psyche and also necessary cooperation with medical personnel. The end of the first part concentrates on the psychosocial factors playing a very important role in the pain perception: understanding the patients, their behaviour and also accepting their responses to their pain. In the second part I list the pain handling therapeutic procedures. I start with the most widely and frequently used methods, then I deal with the newer ones, which are being introduced nowadays. The focus of the last part of my thesis is the patient suffering from chronic pain. I deal with all aspects of human nature, with the problems and difficulties according to M. Gordon. I specify the relevant nursing diagnosis based on the nursing case history. I make a nursing plan containing the goal, nursing intervention, implementation, evaluation, and also education. The purpose of my thesis was detecting the problems of the patients suffering from chronic pain, coming to the solution together and...
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On-line Data Analysis Based on Visual Codebooks
Beran, Vítězslav ; Honec, Jozef (referee) ; Sojka, Eduard (referee) ; Zemčík, Pavel (advisor)
Práce představuje novou adaptabilní metodu pro on-line vyhledávání videa v reálném čase pomocí vizuálních slovníků. Nová metoda se zaměřuje na nízkou výpočetní náročnost a přesnost vyhledání při on-line použití. Metoda vychází z technik využitých u statických vizuálních slovníků. Tyto běžné techniky jsou upraveny tak, aby byly schopné se adaptovat na proměnlivá data. Postupy, které toto u nové metody řeší, jsou - dynamická inverzní frekvence dokumentů, adaptabilní vizuální slovník a proměnlivý invertovaný index. Navržený postup byl vyhodnocen na úloze vyhledávání videa a prezentované výsledky ukazují, jaké vlastnosti má adaptabilní metoda ve srovnání se statickým přístupem. Nová adaptabilní metoda je založena na konceptu plovoucího okna, který definuje, jakým způsobem se vybírají data pro adaptaci a ke zpracování. Společně s konceptem je definován i matematický aparát, který umožňuje vyhodnotit, jak koncept nejlépe využít pro různé metody zpracování videa. Praktické využití adaptabilní metody je konkrétně u systémů pro zpracování videa, kde se očekává změna v charakteru vizuálních dat nebo tam, kde není předem známo, jakého charakteru vizuální data budou.
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Extensions to Probabilistic Linear Discriminant Analysis for Speaker Recognition
Plchot, Oldřich ; Fousek, Petr (referee) ; McCree,, Alan (referee) ; Burget, Lukáš (advisor)
Tato práce se zabývá pravděpodobnostními modely pro automatické rozpoznávání řečníka. Podrobně analyzuje zejména pravděpodobnostní lineární diskriminační analýzu (PLDA), která modeluje nízkodimenzionální reprezentace promluv ve formě \acronym{i--vektorů}. Práce navrhuje dvě rozšíření v současnosti požívaného PLDA modelu. Nově navržený PLDA model s plným posteriorním rozložením modeluje neurčitost při generování i--vektorů. Práce také navrhuje nový diskriminativní přístup k trénování systému pro verifikaci řečníka, který je založený na PLDA. Pokud srovnáváme původní PLDA s modelem rozšířeným o modelování neurčitosti i--vektorů, výsledky dosažené s rozšířeným modelem dosahují až 20% relativního zlepšení při testech s krátkými nahrávkami. Pro delší testovací segmenty (více než jedna minuta) je zisk v přesnosti menší, nicméně přesnost nového modelu není nikdy menší než přesnost výchozího systému. Trénovací data jsou ale obvykle dostupná ve formě dostatečně dlouhých segmentů, proto v těchto případech použití nového modelu neposkytuje žádné výhody při trénování. Při trénování může být použit původní PLDA model a jeho rozšířená verze může být využita pro získání skóre v případě, kdy se bude provádět testování na krátkých segmentech řeči. Diskriminativní model je založen na klasifikaci dvojic i--vektorů do dvou tříd představujících oprávněný a neoprávněný soud (target a non-target trial). Funkcionální forma pro získání skóre pro každý pár je odvozena z PLDA a trénování je založeno na logistické regresi, která minimalizuje vzájemnou entropii mezi správným označením všech soudů a pravděpodobnostním označením soudů, které navrhuje systém. Výsledky dosažené s diskriminativně trénovaným klasifikátorem jsou podobné výsledkům generativního PLDA, ale diskriminativní systém prokazuje schopnost produkovat lépe kalibrované skóre. Tato schopnost vede k lepší skutečné přesnosti na neviděné evaluační sadě, což je důležitá vlastnost pro reálné použití.
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Intrusion Detection in Network Traffic
Homoliak, Ivan ; Čeleda, Pavel (referee) ; Ochoa,, Martín (referee) ; Hanáček, Petr (advisor)
Tato práce se zabývá problematikou anomální detekce síťových útoků s využitím technik strojového učení. Nejdříve jsou prezentovány state-of-the-art datové kolekce určené pro ověření funkčnosti systémů detekce útoků a také práce, které používají statistickou analýzu a techniky strojového učení pro nalezení síťových útoků. V další části práce je prezentován návrh vlastní kolekce metrik nazývaných Advanced Security Network Metrics (ASNM), který je součástí konceptuálního automatického systému pro detekci průniků (AIPS). Dále jsou navrženy a diskutovány dva různé přístupy k obfuskaci - tunelování a modifikace síťových charakteristik - sloužících pro úpravu provádění útoků. Experimenty ukazují, že použité obfuskace jsou schopny předejít odhalení útoků pomocí klasifikátoru využívajícího metriky ASNM. Na druhé straně zahrnutí těchto obfuskací do trénovacího procesu klasifikátoru může zlepšit jeho detekční schopnosti. Práce také prezentuje alternativní pohled na obfuskační techniky modifikující síťové charakteristiky a demonstruje jejich použití jako aproximaci síťového normalizéru založenou na vhodných trénovacích datech.
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Biochemical methods as tool for study of reproductive proteins
Postlerová, Pavla ; Zigo, Michal ; Pohlová, Alžběta ; Jonáková, Věra
Study of molecular mechanisms in reproduction is essential for the understanding of this outstanding process. Our lab studies proteins secreted by reproductive organs and sperm using various biochemical methods for a long time. We have expertise in protein extraction from spermatic cells using different approaches, and by kits for proteins from the sperm surface and distinct subcellular compartments. The proteins of reproductive organ fluids are separated by chromatographic methods, such as size exclusion chromatography, high-performance liquid chromatography with reverse phase (RP-HPLC) and affinity chromatography on matrices with various ligands. Proteins are subjected to SDS- or 2D-electrophoresis for their characterization and comparison of various extraction methods, different mammalian species, and sperm in different functional development. Electrophoretically separated proteins may be transferred onto nitrocellulose membrane (Western blot) for antibody detection or binding studies with lectin-labelled ligands (lectins, polysaccharides, zona pellucida glycoproteins). We use immunoprecipitation method with specific antibody for protein determination followed by the MALDI identification. Proteins are localized by immunofluorescent techniques on/in spermatic cells and tissue sections of reproductive organs. Isolation of proteins from reproductive tissues and fluids, and the antibody detection is crucial for the studying of reproductive protein origin.
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Dynamics of mouse sperm capacitation and acrosome reaction
Dvořáková-Hortová, Kateřina ; Frolíková, Michaela ; Děd, Lukáš ; Šebková, Nataša
Capacitation followed by the acrosome reaction (AR), is a very complex event of molecular changes, including acrosome matrix rearrangement and actin polymerization, which mammalian sperm must undergo in the female reproductive tract in order to obtain the ability to penetrate and fertilize the egg. CD46 and β1-integrin belong to specific proteins, which are predicted to interact during molecular reorganization of capacitating sperm. The IZUMO1 as the primary fusion protein of the mammalian sperm is also involved in this dynamic network. We investigated the relationship between the Izumo, CD46 and β1 integrin relocation in the sperm head during the capacitation and AR in vitro. We have already successfully monitored by immunofluorescent labelling the dynamics of proteins CD46 and β1-integrin. The changes in the localization of these proteins associated with the AR and their mutual co-localization was observed. The original β1-integrin location in the freshly released epididymal sperm is in the acrosome and it relocates during the AR further through the sperm head compartments into the equatorial segment and over the whole sperm head. Its density over the equatorial segment is decreasing with the extended time of the AR. Also its presence in the perforatorium of the mouse sperm head is very prominent. The pattern for protein CD46 is extremely similar if not identical in both aspects such as compartment localization and time progress during capacitation and AR in vitro. The molecular interaction of CD46 and β1-integrin was investigated using the Proximity Ligation Assay and Super resolution microscopy STED. The data were statistically analysed. The newly obtained results from CD46 and β1-integrin relocation are in correlation with IZUMO1 dynamics and giving a substantial knowledge on the studied protein network rearrangement during capacitation and AR in mouse spermatozoa.
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A historical view of the army nurse profession.
PAVLÍKOVÁ, Andrea
The thesis deals with the profession of a military nurse. We can assert that the official study of military, non-medical health-care related qualifications and its use in practice not only for military purposes dates back to the fifties of the last century. However, women as health care professionals, consequently in uniforms, have always been an actual issue. The development of health care activities always existed, however, we must not neglect influence of scientific and medical findings. The historical timeline of development of medical care in the battle field was recorded and ranges from pre-historical healing. The way of Christian medicine was negatively influenced by the Catholic Church. The dark period of the Middle Ages had actually wrested medical performance from the hands of specialists and the suffering were left to the "tender" mercies of the priests. The following part of the thesis concerns modern medicine where enlightenment reforms were exercised. Some of the original six-month courses for military surgeons were organised in this period of time; they weretransformed into Military Surgical Academy, out of which the famous Josefinum originated. A expression of humanity which supported a form of health care of war veterans was demonstrated by constructing buildings for invalid people. The second half of the nineteenth century is characterized by the phenomenon of the presence of nuns in military hospitals. This study goes further in depth of professionally organized healthcare right in the battle field, which dates back from the era of wars in the Crimea.This stage describes the transformation of caring into nursing which requires the necessity of specialized preparation of persons providing nursing practice. In addition, Henry Dunant engraved himself into the history of the profession of a nurse, as the founder of the Red Cross, who first trained health care professionals for working within armed intentions of the state. The continuation of this action sketches out the process of providing first aid in principal armed conflicts of the twentieth century. It describes the functioning of commander operated military health service of that time. WWI drove Czech medical staff to both sides of the front where they provided medical help to the injured. Nurses of the WWII already performed their duties in uniforms as the rightful members of the army. The thesis also mentions post-war usage of military health care professionals in secret foreign missions and their specialized erudition.Last but not least, it marginally discusses contemporary possibilities of modern health care service of the Army of the Czech Republic. The goal of the endeavours of writing this thesis was to chart out clearly the development of the profession of a military nurse within the historical context, focusing mainly on women coming from Czech regions. In order to make the history background concerning specific female participants of the world war conflicts understandable to the reader, this paper reports, in broader extension, peculiar medical and nursing findings, already from the era of prehistoric civilisations. The history study method were applied for the purpose of the theoretical thesis. The methodology is based on searching, studying, sorting and the consecutive systematic objective describing of the events from the past times with the intention to propose comprehensive report on the subject given. It was necessary to study inexhaustible amount of sources to obtain awareness of historical connections. Factual information was derived by analysing of primary and mainly secondary documents. The thesis sketches out histories of military health care and the profession of a military health care professional stemmed from the exploration of appropriate written sources and conclusion of the basic findings. The intentions of the work are not only to clarify the past but also to contribute to solutions of every-day issues.
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Role of physical activity in the prevention of childhood obesity
VRTALOVÁ, Jana
The thesis deals with physical activity in the prevention of childhood obesity. It is focused on children, namely pupils of the eighth and ninith grades of elementary school. Obesity is responsible for more human lives than both of the world wars and if we want this epidemic of obesity and problems associated with inactivity minimized, we have to start where it is most efficient and that is with children. The main objective is to explore physical activity in a selected group of pupils. Partial aim is to determine the prevalence of overweight and obesity in the investigated group of students using somatometric measurements in the selected location. Another objective is to determine how many of childrens parents did sports in the past or actualy do sports. The final goal is to prepare educational material for students about the importance of physical activity. The work is divided into two parts theoretical and practical. The theoretical part was compiled as a secondary data analysis. Sources of data and information originated from the Czech Republic and abroad in the form of scholarly books, journals and electronic resources. The contents of the theoretical part is focused on explenation of terms such as obesity, its consequences, complications, ways to prevent obesity, physical activity, appropriate kinds of physical activity and its effects on the human body. Distinctive part is the chapter dealing with the myths of obesity some of them are really surprising. The second part is practical part and includes its own research. The investigation was conducted in a primary school Komenského alej in Žatec for pupils of the eighth and ninth grades. File featured 107 pupils. The research was conducted as a quantitative survey complemented by measurements of somatometric indicators. The data were collected using a custom non-standardized questionnaire. The questionnaire contained 14 questions and two columns separated by line for recording the exact weight and height. We checked these data at pre-agreed periods.
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