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Non-invasive methods for sex and steroid homones determination in gibbons of the genus Nomascus
Bolechová, Petra ; Bartoš, Luděk (advisor) ; Pavel, Pavel (referee)
The gibbon primates of the family Nomascus are classified as critically endangered species, and, to date, basic understanding and information about their biology is missing. With regard to the status of these animals in the wild and captive populations in zoos, being familiar with their reproduction, may improve captive breeding programs. Data collection in this study was to be carried out by the practical use of non-invasive methods (polymerase chain reaction for DNA extraction and enzyme immunoassays), using faecal samples for analysis. The first method, polymerase chain reaction, was used for sex determination in juveniles, because of their coat colour and visual similarity of secondary sex characteristic in both sexes; it is not possible to determinate sex without handling the animal. Another main purpose of this study is to try and answer the hypothesis regarding the ovarian cycle of females, factors influencing their hormone concentration and also the onset of sexual maturity in females and the timing of their fur colour change. Hypotheses were checked by monitoring the concentration of progesterone and oestrogen faecal metabolites and by evaluation of the composition of breeding groups of gibbons with the ZOO influence. During a four year period (from 2010 till 2014), there were a total of 51 animals analysed from 16 different zoos with a faecal sample count totalling 1618 samples. The results confirmed the use of noninvasive methods for sex determination, and thus ensuring the maximum welfare standards. Endocrinological analysis confirmed the hypothesis of the influence of the environment (ZOO) to the hormone concentrations and female´s pregnancy effect in conjunction with her age on the final results of the male - father hormone concentrations. The initial information in this study is the confirmation of the onset of ovarian cycle in young females without connection to their fur colour change and a significant factor of a mother´s impact and her territoriality. This study is the first to present a long term monitoring of ovarian cycles in females and hormone concentrations of other individuals, both male and female, and from various age groups. The results allow us to understand the possible impact of zoo environments on the reproductive status of these gibbons and contribute to the general improvement of breeding management.
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Query-by-Example Spoken Term Detection
Fapšo, Michal ; Matoušek, Jindřich (referee) ; Metze, Florian (referee) ; Černocký, Jan (advisor)
Tato práce se zabývá vyhledáváním výrazů v řeči pomocí mluvených příkladů (QbE STD). Výrazy jsou zadávány v mluvené podobě a jsou vyhledány v množině řečových nahrávek, výstupem vyhledávání je seznam detekcí s jejich skóre a časováním. V práci popisujeme, analyzujeme a srovnáváme tři různé přístupy ke QbE STD v jazykově závislých a jazykově nezávislých podmínkách, s jedním a pěti příklady na dotaz. Pro naše experimenty jsme použili česká, maďarská, anglická a arabská (levantská) data, a pro každý z těchto jazyků jsme natrénovali 3-stavový fonémový rozpoznávač. To nám dalo 16 možných kombinací jazyka pro vyhodnocení a jazyka na kterém byl natrénovaný rozpoznávač. Čtyři kombinace byly tedy závislé na jazyce (language-dependent) a 12 bylo jazykově nezávislých (language-independent). Všechny QbE systémy byly vyhodnoceny na stejných datech a stejných fonémových posteriorních příznacích, pomocí metrik: nesdružené Figure-of-Merit (non pooled FOM) a námi navrhnuté nesdružené Figure-of-Merit se simulací normalizace přes promluvy (utterrance-normalized non-pooled Figure-of-Merit). Ty nám poskytly relevantní údaje pro porovnání těchto QbE přístupů a pro získání lepšího vhledu do jejich chování. QbE přístupy použité v této práci jsou: sekvenční statistické modelování (GMM/HMM), srovnávání vzorů v příznacích (DTW) a srovnávání grafů hypotéz (WFST). Abychom porovnali výsledky QbE přístupů s běžnými STD systémy vyhledávajícími textové výrazy, vyhodnotili jsme jazykově závislé konfigurace také s akustickým detektorem klíčových slov (AKWS) a systémem pro vyhledávání fonémových řetězců v grafech hypotéz (WFSTlat). Jádrem této práce je vývoj, analýza a zlepšení systému WFST QbE STD, který po zlepšení dosahuje podobných výsledků jako DTW systém v jazykově závislých podmínkách.
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The Pump with a Diffuser with Stochastic Blades (Runner and Diffuser)
Dobšáková, Lenka ; Melichar, Jan (referee) ; Veselý, Jindřich (referee) ; Haluza, Miloslav (advisor)
This doctoral thesis studies the non-uniform blade cascade for centrifugal pumps. Influence of non-uniform blade cascade is investigated on the impeller and diffuser. A non-uniform blading impeller of pumps in this doctoral thesis was examined in terms of influence on efficiency characteristic. The idea of using non-uniform blading is to obtain a flat efficiency characteristic. Then the centrifugal pump could be used on larger scale of power with achieving a sufficiently high efficiency. The work is based on experimental measurements and numerical simulations of non-uniform hydraulic pump components. Non-uniform impeller and the diffuser were newly designed for this research. A new measuring track of small submersible pumps was needed to design and build for measurements. Different pump combinations with conventional hydraulics on one working point and hydraulics with non-uniform were measured. All measurements were evaluated and characteristics of efficiency, head and power were obtained for each measured combination. Some of pump combinations (which were measured in experiment) were numerically simulated by program Ansys Fluent. Data needed to evaluate the characteristic of efficiency and specific energy were collected from each numerical simulation. A theoretical consideration concerns the influence of non-uniform blade cascade applied on diffuser. The suggestion how to decrease hydraulic losses in canals was done. The theoretical consideration how to make a flat efficiency characteristic was made.
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Marketing audit of the festival Divadelná Nitra
Surovková, Simona ; JEŽEK, Vlastimil (advisor) ; SYROVÝ, Daniel (referee)
Divadelná Nitra is an international theatre festival with over 20 years of tradition, which annually brings a selection of the best European productions of the season to Nitra. Visitors are offered a presentation of the top contemporary theatre productions, exceeding to dance, physical, visual and music theatre, new and inspirational art forms, rich accompanying and work programme, and many other art and cultural activities. The festival is an internationally recognized event and is of vital importance in the context of the current Slovak theatre. Yet, as many other non-profit organizations, it has been struggling each year to maintain a stable financial situation, and despite an enormous effort and an experienced management team, the festival cannot avoid some organizational problems. Several of them are related to marketing of the organization. The aim of this marketing audit is to analyze the current situation of the festival Divadelná Nitra, to evaluate the state of its own marketing activities, and, along with discovered results, to propose solutions that could help to improve marketing of the festival Divadelná Nitra.
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THE DRAMATICAL SCRIPT AS A NECESSARY BASIS FOR SPACIAL REALIZATOIN
Tretiag, Štěpán ; KORČÁK, Jakub (advisor) ; HRBEK, Daniel (referee)
Using the concrete examples of my practical bachelor work my intent is to retrospectively describe and reflect on our scenic realization of the play Missing by south-African author Reza de Wet. The essence of a modern dramatic direction is to perceive the script of a play as a model which becomes a basis for its interpretation and the subsequent staging of the play. It is the process from the initial interpretation to final scenic realization I wish to focus on in my work.In the first part I will be dealing with creation of a physical space, that being stage design. I will try to capture what lead us to its final shape and how the basis for that can be found in the script of the play as well as the potential capability of the physical space to become a dramatic space. Its ability to create a platform for the actors to proceed from and how can it provoke them into acting, in particular. In the second part I will be dealing with the dramatic space, ergo the spacial expression of relationships and themes. I will proceed again retrospectively, focusing on how we managed to achieve the stage image which is functioning as a materialized metaphor. I will focus mostly on non-verbal actions, but with regard to the fact, that dramatical script is the basis for our realization of the play.
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Specifications of nursering care of patiens with complications after direct SKG/PTCA
BLÁHOVÁ, Ilona
Abstract In the Czech Republic there is no doubt about the tendency of gradual increase in median life expectancy, which is significantly affected by the fact that mortality from cardiovascular diseases, especially from acute coronary syndromes, has been decreasing. Besides the provable effect of a healthy lifestyle, diet and, by all means, a quality and effective pharmacotherapy, a significant development in the field of interventional cardiology contributes to this accomplishment. The number of coronographies, coronary angioplasties and implanted stents have multiplied, and today the invasive coronarographic diagnostics and percutaneous myocardial revascularization belong to the the most common diagnostic and therapeutic methods in treatment of acute forms of ischemic heart diseases. An obvious prerequisite for such a rapid development in the field of intervention coronary angiography was the establishment of a sufficiently dense network of catheter laboratories and specialized facilities, which provide a highly professional and intensive care for patients. This thesis is focused on three basic objectives: ? To survey and characterize differences in nursing care concerning various complications in patients after SKG / PCI ? To survey bio / psycho / social impacts of complications after SKG / PCI on a patient ? To identify and summarize personal and material prerequisites and requirements to ensure quality nursing care for these complicated conditions The research was conducted by using a qualitative methodology. The methods used were observation, non-standardized interviews and medical and nursing records analyses. The research survey samples on which the investigation was focused were patients with the acute coronary syndrome hospitalized in the coronary care unit in the Cardio Center in České Budějovice, their family members and also the nursing staff providing the comprehensive nursing care. The outcomes of this survey were eleven descriptive case reports characterizing the occurrence of the most frequent complications in patients with ACS after SKG / PCI. To ensure clarity, each case study is complemented by a thought map with an account of the most important nursing interventions in the management of specific acute conditions. The paper also contains a framework analysis of bio / psycho / social impacts of complicated situations on patients. It is interesting to compare this matter from the perspective of nurses and patients, which is seen in correlation graphs. The section describing the organizational and personnel provision is introduced with the characteristics of the medical process and it also contains the list of medical personnel with their qualifications and the length of experience in the Coronary care unit in České Budějovice. Summarization of the instrumental medical equipment is also based on the analysis of previous cases and is accompanied by photographs of the equipment typical and indispensable for the care of patients in the Coronary care unit, which primarily has an informative and complementary character to get an integrated view of the Coronary care unit running and the nursing staff work.
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The Importance of the Fundraiser in a Non-profit Organization
STARÁ, Radka
The main theme of the bachelor work is the importance of the fundraiser in a non-profit organization. It defines the term fundraising, its principles, forms and methods, the work of a fundraiser in a non-profit organization.The work characterizes nonprofit organizations, their legal status and field of activity. There are also information about activities, which improve economy and financial situation of these organizations. In the practical part of my work I have analyzed the way of financing a non-profit organization Junak- Czech scout, Centre Pod Kleti Holubov z.s. I am comparing in this part the way of fundraiser work on the local and state platform. The facts here available are drawn from the public web pages of this organization and also from the account keeping documents of its local center. At the conclusion of my work I am assessing the effectiveness of fund raising plan and its strategy and also describing the way of negotiation with donors. I am noticing the need of a fund raising team creation and also rules of fundraising including necessary cooperation between the center of organization and its local branches.
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Estrogen receptor beta (ERβ) in testicular cells and sperm
Dostálová, Pavla ; Žatecká, Eva ; Děd, Lukáš ; Dorosh, Andriy ; Postlerová, Pavla ; Jonáková, Věra ; Dvořáková-Hortová, Kateřina ; Pěknicová, Jana
Estrogen is a steroid hormone that plays an important role during sperm development in the male and female reproductive tract. Estrogen signalling is a complex process that depends on cell milieu and presence of receptors. Thanks to the steroid nature of estrogens, they can pass through the plasmatic membrane and bind to the intracellular estrogen receptors (ERs). Within the cell, there are several pools of ERs. One of them is localized to the cell nucleus and their activation leads to direct or indirect binding to DNA and ultimately to alternation in gene expression (genomic pathway). Other pools of ERs are associated with plasma membrane or are located in cytosol. Activation of membrane associated ERs leads to rapid non-genomic responses. Nowadays, two classical estrogen receptors are known – ERα and ERβ. Since ERβ is a predominant variant in testes, we focused our study on expression of ERβ variants in murine testes and sperm. We detected two variants of ERβ at mRNA level in both, testes and sperm. These variants differ in 54 nucleotids within the ligand binding domain and this variability results in different affinity to estrogen. We analyzed individual testicular cell types (spermatogonia, spermatocytes, spermatids, Sertoli cells) by RT-qPCR. Our results suggest that both ERβ variants are coexpressed in the same cell type and may therefore interact together. This may have consequences in mediating of estrogen signalling. Moreover, ERβ is expressed more in the later stages of spermatogenesis suggesting the role of ERβ in these stages or alternatively in spermatozoa alone. At the protein level, we detected ERβ in nuclear, membrane and cytosolic fraction prepared from testicular tissue suggesting the involvement of both, genomic and non-genomic, pathways of estrogen signaling in testes. In sperm, anti-ERβ antibodies localized ERβ in acrosome region and tail which is in accordance with the known role of estrogen on capacitation, acrosome reaction and motility.
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Monetary Policy and Macroprudential Policy: Rivals or Teammates?
Malovaná, Simona ; Frait, Jan
This paper sheds some light on situations in which monetary and macroprudential policies may interact (and potentially get into conflict) and contributes to the discussion about the coordination of those policies. Using data for the Czech Republic and five euro area countries we show that monetary tightening has a negative impact on the credit-to-GDP ratio and the non-risk-weighted bank capital ratio (i.e. a positive impact on bank leverage), while these effects have strengthened considerably since mid-2011. This supports the view that accommodative monetary policy contributes to a build-up of financial vulnerabilities, i.e. it boosts the credit cycle. On the other hand, the effect of the higher bank capital ratio is associated with some degree of uncertainty. For these and other reasons, coordination of the two policies is necessary to avoid an undesirable policy mix preventing effective achievement of the main objectives in the two policy areas.
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Experiences of health workers with acupressure
ROUBÍČKOVÁ, Radka
The abstract of the diploma thesis is focused on the acupressure and its influence of backache of medical staff. We chose the theme of acupressure because it is a non-invasive treatment method which can be practiced by everyone in the comfort of the home and no aid is necessary to have, just the basic knowledge and training how to perform acupressure. Acupressure is not physically or time consuming and that is why it is considered as a suitable means as an additional treatment of pain and other health disorders. It can improve nursing care. The theoretical part deals with pain and pain of medical staff who are the highest risk group of backache in the lumbar region. The backache is the most frequent cause of incapacity for work. Furthermore, in the theoretical part we deal with acupressure, one of the methods of alternative medicine. For the processing of the theoretical part, we used the available foreign and Czech literary sources. The empirical part of the diploma thesis is a quantitative method. The results obtained from the research were processed in the overview tables and case reports. For the thesis we set two research objectives. The first objective was to find out the experience of medical staff with acupressure. The second objective was to verify the influence of the techniques of acupressure for backache of medical staff. On the basis of the aim two hypotheses were created. The first hypothesis should show whether the backache will be softened by application of the acupressure techniques. The second one shows whether there is the difference in approach of nurses and doctors to perform acupressure.
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