|
Antioxidant, anti-proliferative and immunomodulatory effect of fruits, vegetables and mushrooms in vitro
Doskočil, Ivo ; Havlík, Jaroslav (advisor) ; Orsák, Matyáš (referee)
Recently there has been an increasing interest in discovering of new species of plants and mushrooms which have antioxidant or anti proliferative activity. The interest is caused by the fact that these species have medicinal and food utilization. These properties of plants and mushrooms can be used when dealing with many diseases which may be connected with oxidative stress (inflammatory bowel disease, cardiovascular disease, hypertension, and tumour disease, etc.).
The thesis propounds characteristics of in vitro antioxidant and anti proliferative activity of 19 types of juices and 28 methanol extracts of fruits and vegetables, which are common parts of a diet. In the thesis there are also characteristics of 13 ethanol extracts of edible mushrooms of the order of Polypore (Popyporales). Antioxidant activity was quantifie by 2, 2 diphenyl 1 picrylhydrazyl (DPPH), oxygen radical absorbance capacity (ORAC), and inhibition of nitric oxide (NO) production. Cytotoxicity was measured by MTT (3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyltetrazolium bromide. Immunomodulatory activity was determined by an assay of phagocytic activity of human neutrophil granulocytes. Further a total phenolic content (TPC) and total beta glucan content were investigated; these are considered to be the potentially active constituents participating in mentioned activities.
From the obtained results it is evident that juices (TPC = 1603.2 mg GAE/L; ORAC = 438.5 umol TE/g)
and a bilberry (Vaccinium myrtillus) fruit extract (ORAC 836.6 umol TE/g; DPPH = 404.6 umol TE/g) showed
the highest antioxidant activity of all tested samples of fruits and vegetables. Capsicum (Capsicum L.)
juices (TPC = 642.1 mg GAE/L; ORAC = 127.9 umol TE/g) and a radish (Raphanus sativus L.) extract
(ORAC 724.5 umol TE/g; DPPH = 52.2 umol TE/g) also proved to have the high values of antioxidant activity. From the tested fruits and vegetables the following inhibited to produce a nitric oxide: onion (Allium cepa L) juices (lowered the NO production by 57%), tangerine juices (Citrus reticulata Blanco)(by 52%), broccoli (Brassica oleracea var. botrytis italic) extract (by 21%), and orange (Citrus sinensis Pers.) extract (by 10%). Concerning edible
mushrooms, Lentinus tigrinus (Bull.) Fr. (TPC = 216.2 umol GAE/g of extract), Ganoderma lucidum (Curtis) P. Kras (TPC = 257.9 umol GAE/g of extract), and Royoporus badius (Pers.) A.B. De (TPC = 257.8 umol GAE/g of extract) presented the highest phenolic content. Sparassis crispa (Wulfen) Fr. (117.4 mg/g of extract) had the highest content of beta glucan. Substantial effect on phagocytic activity of granulocytes was noticed in connection with Neolentinus lepideus (Fr.) Redhead & Ginns, Polyporus squamosus (Huds.) Fr., and S. crispa. In connection with the latter of mentioned mushrooms, a moderate inhibitory effect towards HT 29 human colorectal adenocarcinoma cell line
(IC50 = 107 ug/ml of extract) was noticed.
The results indicate that the tested plants and mushrooms can be beneficial when reducing the negative effects of oxidative stress. The oxidative stress has been recognized as a contributing factor to a whole range of diseases and the reduction of the oxidative stress may lead to the decrease in the possibility of the diseases´ progression. The results show the possible favourable effects on the human health. When evaluating the results, the following fact should be taken into consideration; the in vitro tests and screenings are considered to be the first phase of systematic research of the effects and serve for the choice of respondents for further detailed studies.
|
|
Quantitative correlation of textural data obtained with CIP and EBSD method
Slunská, Petra ; Lexa, Ondrej (advisor) ; Machek, Matěj (referee)
Since 2011, the Institute of Petrology and Structural Geology, Charles University in Prague, worked with CIP - Computer Integrated Polarization microscopy as fast, inexpensive measurement of c-axis orientation of uniaxial minerals, mostly quartz in high definition. CIP method is developed from the early nineties in Switzerland (Heilbronner & Pauli 1993) and later in many other workplaces. The aim of this work was testing and calibration of optical and camera equipment to verify the accuracy and reliability of data obtained. Served as an independent measurement of EBSD (Electron Backscatter Diffraction) data obtained from the same part of the studied thinsections. The data obtained were analyzed by quantitative analysis of microstructures (PolyLX - MATLABTM toolbox; Lexa 2003). The samples used for testing the methodology mentioned were taken on the profile of Hvězdná and Zdobnice near Rokytnice in the Eagle Mountains by contact orlica-snieznik complex and its mantle. Field studies showed the existence of west dipping shear zone along the said contact and deformed orthogneiss show a macroscopic superposition of several deformation events. The resulting frequency histograms similarities and differences of angles c-axes and angles misorientace grains have a high consensus in the azimuthal criterion, axes...
|
|
Packet Classification Algorithms
Puš, Viktor ; Lhotka,, Ladislav (referee) ; Dvořák, Václav (advisor)
Tato práce se zabývá klasifikací paketů v počítačových sítích. Klasifikace paketů je klíčovou úlohou mnoha síťových zařízení, především paketových filtrů - firewallů. Práce se tedy týká oblasti počítačové bezpečnosti. Práce je zaměřena na vysokorychlostní sítě s přenosovou rychlostí 100 Gb/s a více. V těchto případech nelze použít pro klasifikaci obecné procesory, které svým výkonem zdaleka nevyhovují požadavkům na rychlost. Proto se využívají specializované technické prostředky, především obvody ASIC a FPGA. Neméně důležitý je také samotný algoritmus klasifikace. Existuje mnoho algoritmů klasifikace paketů předpokládajících hardwarovou implementaci, přesto však tyto přístupy nejsou připraveny pro velmi rychlé sítě. Dizertační práce se proto zabývá návrhem nových algoritmů klasifikace paketů se zaměřením na vysokorychlostní implementaci ve specializovaném hardware. Je navržen algoritmus, který dělí problém klasifikace na jednodušší podproblémy. Prvním krokem je operace vyhledání nejdelšího shodného prefixu, používaná také při směrování paketů v IP sítích. Tato práce předpokládá využití některého existujícího přístupu, neboť již byly prezentovány algoritmy s dostatečnou rychlostí. Následujícím krokem je mapování nalezených prefixů na číslo pravidla. V této části práce přináší vylepšení využitím na míru vytvořené hashovací funkce. Díky použití hashovací funkce lze mapování provést v konstantním čase a využít při tom pouze jednu paměť s úzkým datovým rozhraním. Rychlost tohoto algoritmu lze určit analyticky a nezávisí na počtu pravidel ani na charakteru síťového provozu. S využitím dostupných součástek lze dosáhnout propustnosti 266 milionů paketů za sekundu. Následující tři algoritmy uvedené v této práci snižují paměťové nároky prvního algoritmu, aniž by ovlivňovaly rychlost. Druhý algoritmus snižuje velikost paměti o 11 % až 96 % v závislosti na sadě pravidel. Nevýhodu nízké stability odstraňuje třetí algoritmus, který v porovnání s prvním zmenšuje paměťové nároky o 31 % až 84 %. Čtvrtý algoritmus kombinuje třetí algoritmus se starším přístupem a díky využití několika technik zmenšuje paměťové nároky o 73 % až 99 %.
|
|
(Arbo)viruses in high European Arctic
ELSTEROVÁ, Jana
Since an ongoing climate change covers strongly the polar areas. Higher temperatures and related climate parameters bring the emergence of new parasites and their pathogens to higher latitudes. This may influence zoonotic diseases including arthropod-transmitted diseases. The tick species Ixodes uriae, parasitizing seabirds in the Arctic, may transmit many pathogens including various arboviruses, Borrelia spirochetes and Babesia apicomplexans. In the study we diagnosed 89 individuals of seabird tick Ixodes uriae and searched for arthropod-borne viruses from the genera Flavivirus, Alphavirus, Orthobunyavirus, Phlebovirus and Orbivirus using genus-specific primers. Moreover we searched for presence of Borrelia spp. and Babesia spp. DNA in the ticks. All samples were negative after PCR amplification for all tested pathogens. The result signalizes that tested pathogens might not be present in such high latitudes of European Arctic. Though, the possibility of introduction of these pathogens may be observable in near future due to quick changing of the Arctic ecosystem with the rising of migration of vertebrate hosts including humans to the polar areas. It is though important to continue to study potential presence of pathogens in polar areas. We are working on increase of the number of tick samples to confirm the presence or absence of the tick-borne pathogens in the European Arctic.
|
|
Právní a zdravotně sociální aspekty činnosti OSPOD jako ustanovených opatrovníků v zámu nezletilých dětí
BORSKÁ, Jana
The Czech Republic as a signatory of the Convention on the Rights of the Child has vested the practice of state administration in the field of care for minor children to municipalities with extended powers, where the protection of the rights and legitimate interests of minor children is safeguarded by state authorities of social and legal protection of children (ASLPC hereinafter). These authorities are incorporated to the extent laid down by the Act on Social and Legal Protection of Children (Act No 359/1999 Coll., as amended). The position and roles of ASLPC and their competence and jurisdiction are also regulated by the same act. The position of workers of these authorities and its legal embodiment is equally important. Regarding professionality, there are high demands on these workers. In the Czech Republic, significant changes in legislature have been made over the past three years. These amendments have led to the strengthening of the rights of minor children and to the establishing of new tools for their protection. In connection with the adoption of new substantive regulation of family law, which is now exhaustively dealt with by the Act No 89/2012 Coll., the Civil Code, new procedural rules have been established regarding protection of rights of minor children. Such procedural legislation includes (apart from the long-existent Act No. 99/1963, the Code of Civil Procedure) the Act No 292/2013 Coll., on Special Civil Proceedings. The decision-making regarding minor children has been vested mainly by the state into the competence of courts. The courts appoint the locally relevant ASLPC as a guardian who then represents the interests of minor children. The purpose, interwoven with and derived from the first one, was to explore the opinions of selected social workers of ASLPC and of district court judges regarding the defined problems occurring during the work of ASLPCs and courts while protecting the interests of minor children. These particularly include: the use of the tool of precautionary measure; problems of different territorial jurisdiction of courts and ASLPCs; processing of complaints regarding bias in various phases of the proceedings; professional training of workers of ASLPC It is evident from the proposals of both judges and ASLPC workers that it is necessary to unify territorial jurisdiction. The courts are suggesting the priority criterion of the address where the child mainly resides. ASLPC workers suggest the official permanent residence as the prime criterion. Bias causes problems in all phases of proceedings. It is obvious that courts take differing approaches towards the solution of this problem (some courts solve the problem of bias complaints filed by the parties while other courts do not) and for ASLPC workers it is difficult to assess how to act when such complaint is raised against them. The training of ASLPC workers - workers are obliged to educate themselves and their employer is obliged to finance such training. Not all ASLPCs are able to provide training in the required extent - due to financial reasons (the average costs of education of one employee pose from 9,169 to 13,400 CZK annually and they cover approximately 6 days of training). The heavy work-load caused by insufficient numbers of ASLPC employees prevents them from completing such compulsory training. As part of the examination of the "participation of a collision guardian in court proceedings" it has been found out that irregular participation of the worker in the proceedings (absence from participation in appeals proceedings); incomplete reports (directed to courts) from surveys in families. Based on comprehensive examination of the problems of territorial jurisdiction, it was recommended that requests are independently recorded and financial compensation is provided for ASLPCs who are requested to represent minors in front of courts, to draft proposals for precautionary measures.
|
|
Adolescent overweight and obesity
JINDROVÁ, Kateřina
My bachelor´s thesis deal with obesity of adolescents and problems with overweight. These problems are described in details, and after that divided into specific parts. The main goal of my bachelor´s thesis is to chart problems with obesity and overweight in age group from 15 to 18 (adolescents) and primary describe their eating habits and physical activity. Theoretical part is focused on occurrence of overweight and obesity in the Czech Republic and in the world, causes of its origin, risk factors, classification and diagnosis, types, therapy and prevention. This bachelor´s work dealing with rational diet, describing basic principles of healthy catering and explaining right or wrong foodstuffs. I point mainly on prevention of overweight and obesity by healthy eating and physical activity. Methodical part is processed thanks to quantitative research. Data were gained by questionnaires, which were given to high school students (1st - 3rd grade). The research group contains 150 respondents from different schools in České Budějovice and close neighborhood. Final results are written down primary in graphs with commentary and data charts. In case of right use of qualitative research, there were determined two hypotheses. First hypothesis: Overweight and obesity is more occurred at adolescents with wrong eating habits. Second hypothesis: Overweight and obesity is more occurred at adolescents with low physical activity. For developing exploratory part, there was used quantitative research, which was provided by questionnaires given to students of age from 15 to 18. Entire data were processed by the use of program Microsoft Excel, and its calculations and functions. The answers of respondents were registered into database, and, by using specific excel functions, I was able to report data in determined categories. The research was mainly concentrated on physical activity and eating habits. Final results are put into well-arranged graphs, which are always enriched by comments, eventually completed by data tables, and, subsequently, statistically evaluated. In results, there might be seen two main differences between adolescents with standard weight and adolescents with overweight or obesity problems. One of those is irregularity in eating and wrong eating habits; big difference is also proved in physical activity. Adolescents with obesity or overweight have often irregular food intake, they drink a lot of high caloric beverages such as Coca-Cola, Sprite, Fanta etc., deficient income of fruit, vegetables, legumes, fish, or low physical activity. Only few of obese adolescents is different, because their problem is caused genetically. Adolescents with standard weight eat more regularly, but there are also some special cases, who have similarly wrong eating habits as obese people; however, all of these adolescents have one typical quality regular physical activity. Both hypotheses are confirmed. The results show that obesity is mainly caused by genetic predispositions, but these factors can be positively affected by periodic physical activity or appropriate eating habits. At the end of the thesis, it should be mentioned that it is necessary to be focused on right eating habits and regular physical from the very young age, when the basic customs and habits are assumed. That should include parents' action around their children, as they are usually the first example and they have the biggest influence.It is important to develop knowledge about all kinds of prevention, which are in most cases major in defending against formation of overweight and obesity.
|
|
Matematická analýza regularizovaného modelu viskoelastické nenewtonovské tekutiny
Šalom, Pavel ; Pokorný, Milan (advisor) ; Bulíček, Miroslav (referee)
In this thesis we provide an existence result for a regularized model of viscoelastic non- newtonian fluid. We consider incompressible fluid with shear rate dependent viscosity and with Cauchy stress tensor capable to describe stress relaxation. An elastic part of the Cauchy stress tensor is governed by Oldroyd-type differential equation. In particular, we are interested in fluids with strong shear thinning effect. We prove that if the viscosity function µ (D) is such that tensor µ (D) D is p-coercive, monotone and has (p − 1)-growth for p > 6 5 and some other additional assumptions are satisfied, then there exists a solution to the system of PDEs describing the flow in a bounded domain. The proof is not simple because the convective term is not integrable with a high power. The problem is solved using Lipschitz truncation method for evolution PDEs. 1
|
|
High normal pressure and hypertension of children and adolescents
KRÁLOVÁ, Kateřina
In children and adolescents, high normal blood pressure is often present. People with high normal blood pressure show a short time prerequisite to the formation of arterial hypertension. Hypertension occurs most often in children as asymptomatic. Increased blood pressure in children is most often discovered during preventive examinations by a general practitioner for children and adolescents. This thesis contains information about the anatomy and physiology of the cardiovascular system, a blood pressure measurement sister, evaluation of blood pressure, high normal pressure, high blood pressure in children, the role of nurses in primary care pediatricians in the area of communication and education, and treatment of high normal pressure and hypertension. Objectives The first goal was to explore how children adhere regime measures for the prevention of high normal pressure and hypertension. It used this research question: How children adhere regime measures for the prevention of high normal pressure and hypertension? The second objective was to determine the role of nurses in the care of children with high normal pressure and hypertension. The second research question is: What is the role of the nurses in taking care of children with a normal high pressure and hypertension? Methods used In the research part of the thesis, we used the method of qualitative research, questioning method and technique-depth interviews. The interviews were recorded on a Dictaphone and then transcribed literally. Four sisters and three mothers did not wish to be recorded, so they were interviewed at polling literally written on paper. For research, two research files were created. The first sample consisted of six nurses, who were working in GP surgery for children and adolescents in the South Region. Second survey consisted of six people selected from among parents of children with high normal pressure and hypertension, also from South Bohemia. Results The transcribed interviews were investigated by open coding method, pencil and paper. Resulted in 9 categories: Cardiovascular disease, role of the nurse, pressure measurement, blood pressure, hypertension, pressure measurement, recommendation, Lifestyle, Leisure. Individual categories were assigned to subcategories, which are encoded core data. Conclusion The first research confirms nurses working in a pediatrician's office for children and adolescents, the most common cardiovascular disease in the general hypertension. They argue that the role of nurses in primary care pediatricians is properly measure blood pressure and make the education of children and parents in the area. The respondents concurred in the principles of how to properly measure blood pressure measurement are available for at least three cuff sizes. Informants have basic information about high normal pressure and hypertension, and said that, in the offices of primary care pediatricians to conduct checks blood pressure of preventive examinations or troubled child. Informants reported that blood pressure control and education on blood pressure occurs, but in the second research study showed that children and adolescents do not comply with the recommended recommendations in this area, perhaps because they are less motivated. Neither sister nor the parents have not mentioned, they would receive educational aids such as brochures and sample menus.The second research showed that parents most often found elevated blood pressure in primary care pediatricians, either for reasons of preventive examinations or difficulty a child. On the control measurements came at least three times. Respondents agreed on the recommendations they received in the office GPCY such as reducing the weight of the child, regular physical activity, restriction of salt and proper diet the child, but not all children and adolescents comply with these recommendations. The research results will be provided to the journal Pediatrics practice.
|
|
Nursing care in a hygienic and epidemiological regime from the patient's perspective.
KŘEPELOVÁ, Lenka
Considering contemporary lifestyle and possibility of travelling imported infections accrued and some illnesses come back that have not occurred nearly at us more. Not only haemorrhagic fevers and other highly infectious illnesses but also hospital multiresistant bacterial strains that mean high risk for patients constitute a threat. The number of nosocomial infectious grows still. The patient is in the case of suspicion or already diagnosed infectious illness isolated and treated in the infectious department or in the tribal department in the room with increased hygienic-epidemiologic regime. The patient is saved in a single room, the possibility of visits is reduced and the staff uses personal protective equipment. The man is holistic being and it can come due to isolation to lack of satisfaction of his/her bio-psycho-social needs. The aim of this thesis is to identify unsatisfied bio-psycho-social needs of patients in increased hygienic-epidemiologic regime and to describe changes in needs of the patient depending on the time of hospitalisation. The theoretic part describes the problematics of infectious illnesses, system of the care for the patient with infectious illness and changes in human needs in the period of the illness. The empiric part of the thesis deals with unsatisfied bio-psycho-social needs of isolated individual through quantitative research. The research suite was made up of 10 patients who were hospitalised in the room with increased hygienic-epidemiologic regime. The survey was carried out in the Hospital Jihlava by semistructured interview and the results of the survey were processed by opened coding by method paper and pencil. It is emphasized that the most needs of the patient have biological, psychical, social and even spiritual aspects. The rate of satisfaction with caregiving depends on the health condition and personality of the patient. Not small influence has the length of the hospitalisation, too. It was found out during the survey that the patients have not enough information about specifics of the care in the infectious department and that is why there is often a misunderstanding of necessity of barrier measures. It is possible to avoid useless misunderstanding through timely and suitable education. It was created an educational material with the title "Guide for patients hospitalised in the infectious department of Hospital Jihlava" for this purpose. Employees of the infectious department of Hospital Jihlava were informed with results of the research during a workshop, too.
|
|
Poliomyelitis and contemporary issues of its eradication
PETRÁŇOVÁ, Monika
This diploma thesis deals with the issue of the eradication of infectious polio and its current problems. Polio is a highly infectious disease of viral origin. The most effective form of protection from the disease is vaccination. The main aim of this work was to determine the major issues in polio surveillance in the Pilsen region at present. Intermediate goals were determined in relation to the main aim: to ascertain the awareness of practicing paediatricians about the principles of poliomyelitis surveillance with an emphasis on diagnosing and reporting cases of acute palsy and further to ascertain the quality of the cooperation between the locally competent public health authority and practicing paediatricians in the field of poliomyelitis surveillance. The work is divided into two parts, theoretical and practical. The practical part was elaborated using a qualitative method based on semi-structured interviews with practicing paediatricians and with the locally competent public health authority. The research was participated in by 9 practicing paediatricians and one public health authority worker from the Pilsen region, with an average length of practice of 20 years. The resulting data was then evaluated by a coding method and divided into schemes according to Švaříček and Šeďová (2007). Three research questions were defined based on the set aims: RQ1: What do practising paediatricians see as the prime problems in polio surveillance? RQ2: How many cases of acute palsy do practising paediatricians record and subsequently report to the competent public health authority? RQ3: What is the quality of the cooperation between practising paediatricians and the locally competent public health authority? The research revealed that, according to the practising paediatricians, the problem in poliomyelitis surveillance is refusal of the vaccination and the associated decreasing immunisation coverage of the population. The next most commonly reported problem was population migration. Furthermore, it was found that not one practising paediatrician recorded or reported cases of acute palsy in their surgery. Only one respondent encountered acute palsy 10 years ago. The answer to the last research question is also apparent from the information obtained. More than half of the respondents agreed that so far there is no cooperation on this issue. Although four respondents stated that the cooperation is at a high level and is of very good quality.
|
guest ::
