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Účinky xenobiotik na oxidační stres, metabolizmus lipidů, integritu DNA a životaschopnost lidských buněk a rybích spermií in vitro
LINHARTOVÁ, Pavla
Pollution of the aquatic environment by inorganic and organic chemicals is a major factor posing a serious threat to the survival of aquatic organisms including fish. In addition balancing risks and benefits of fish consumption is nowadays an intensively discussed public health topic. Spermatozoa of almost all fish species are released into water environment where they can be directly exposed to various compounds, such as xenobiotics including toxic metals, prior to fertilization. In addition, exposure of parental adults to various xenobiotics may affect gamete quality, which may subsequently reduce fertilization success. On the other hand the advantages of eating fish are well-known, not only in the point that fish is a healthy source of protein and other nutrients, but eating contaminated fish may also confer various health benefits. Research over the past few decades has shown that the nutrients and particularly the n-3 fatty acids (FA) found in fish and seafood, are for examples protective against cardiac diseases and have a positive impact on brain development. The thesis provides a focus on two different cell model types. Firstly, human hepatocellular cells (Hep G2, ATCC) were used as in vitro tool for studying the effect of the intake of cadmium (Cd2+) contaminated fish on cytotoxicity, oxidative stress and fatty acid and phospholipid class compositions. Secondly, spermatozoa of one threatened species of fish, sterlet (Acipenser ruthenus) were used as in vitro model for studying effect of potentially hazardous xenobiotic compounds' occurring in open waters. Sperm from sterlet were exposed for 2h to environmentally relevant concentrations of DQ (0-150
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Summary of foreign knowledge about the origin and development of EDZ in crystalline rocks - research
Vavro, Martin ; Souček, Kamil ; Staš, Lubomír ; Vavro, Leona
Presented search summarizes findings of foreign research oriented on the origin and evolution of the excavation damaged zone in crystalline rocks with a particular focus on the essential results of experimental projects which were performed in Canada, Sweden, Finland, and Switzerland. The study is divided, excluding the introduction, into seven main chapters of the text, which gradually deal with: (1) definition of key terms, (2) overview of the main underground research laboratories in the world where EDZ assessment was conducted, (3) methods suitable for EDZ description and characterization, (4) main factors influencing the origin of failure around the excavations and time-dependent evolution of EDZ. An overview of important outcomes of EDZ experiments, focusing on the European hard rock laboratories (Stripa, Äspö, Onkalo/Olkiluoto and Grimsel), and their summary are presented in the final two chapters.\nThe review summarizing the published key findings and results of in situ experiments shows, that for rock in lower stress state, i.e. in no spalling environment, the extent and character of rock mass damage is typically dependent on the excavation method. Using mechanical excavation, rock damage zone with thickness less than 3 centimeters can be originated. The microcracks within this zone contribute to the increase of hydraulic conductivity of the rock mass. At some test sites (Äspö, Grimsel), where the tunnel boring machine technology was used, the damage zone was already detected in depth of less than 5 mm.\nOn the contrary, openings excavated by drilling and blasting are characterized by much more extensive damage zones up to several tens of centimeters in width. The damage progressively diminishes with the distance from the opening.
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Usability of connectivity metrics as predictors of species distribution
Kučera, Michal ; Šímová, Petra (advisor) ; Krčílková, Šárka (referee)
The aim of this thesis is to evaluate whether connectivity indices are usable for bird prediction.
The study area contains of the whole country but is divided into separate squares with area of
133 km 2 each, where the presence or absence of the bird species is known. The dependence is
tested on landscape connectivity indices presented by prof. S. Saura. The land cover contains
of Corine land cover 2012 data. The results will be evaluated with logistic regression approach
on sample data including 4 different dispersal distances (500 m, 1 000 m, 2 000 m and 3 000
m) and 10 specific bird species typical for given land cover. The corelation between land cover
area presence of the bird is also tested.
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Motivation as a leadership
Řezáčová, Kateřina ; Fiedler, Jiří (advisor) ; Horáková, Jana (referee)
The bachelor thesis discusses topic Motivation as a leadership. The work is divided in two main parts, theoretical part and practical part. Theoretical part is based on literature review, which describes theoretical concepts related to issues of leadership and management. Explained in detail here are concepts like management, managerial functions, leadership, leadership styles, motivation, stimuli, personality determinants, incentive mechanism and motivational system. Practical part focuses on management skills. The aim is to compare experiences and stands of four managers, who work at The Ministry of Education, Youth and Sports with assistance of guided interview. Objective of this part is to find out which way these managers motivate and lead their employees.
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Importance of communication elements for management
Nováková, Zdeňka ; Fiedler, Jiří (advisor) ; Stanislav, Stanislav (referee)
The diploma thesis deals with the importance of various elements of communication for work of manager. Communication is important and we use it in private and in working life. This thesis is divided into two main parts.
The first main part includes an overview of the solved problem that is treated on the basis of scientific literature listed in the bibliography. It contains an explanation of the concept and its management, management level, manager, managerial functions, managerial roles and leadership. Next is analyzed in detail the concept of communication, verbal and nonverbal forms and their further division. Finally there are elements of an effective communication, communication channels and the most frequent mistakes in communication.
The second main part includes data obtained from a questionnaire survey of managers. Follow their analysis and statistical surveys. In conclusion these results are evaluated.
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Environmental Impact Assessment Follow up analysis of Pankrác administrative centre
Franclová, Alena ; Keken, Zdeněk (advisor) ; Karel, Karel (referee)
This diploma thesis evaluates issues of environmental impact assessment, focusing on post-project analysis of the Pankrác administrative center. Follow-up evaluation is one of the most important practice through EIA which can fulfil one's potential. Major contribution is mainly the EIA feedback which will be compared between real and predicted impact. Follow-up will provide knowledge to avoid possible non-conformances in EIA evaluation process in the future. Methodical of this thesis is based on international standards of good practice in EIA follow-up, which should always correspond with the specific circumstances of the project and the specific context. The main principles are results-oriented approach, learning, institutional commitment and responsibility. Meaning of the follow-up is the main research question to evaluate the environmental impact assessment. The thesis evaluates the implementation of the main comments from the various point of views. All opinions, changes and views of all participants must be reviewed in implementation and operation phases of the EIA process. Another sub-section provides a comparison of the noise pollution before and after realization of the project and analytical part concludes evaluate compliance with the general principles of good practice. The results collect comprehensive assessment of the EIA process. The follow-up for Pankrác administrative center was important and it proved that positive cooperation with the investor made processes more effective in the terms of the environmental protection of the area. In the future it would be appropriate to dedicate more effort and time for the follow-up. Especially raising awareness of the existence and positive impact of the entire EIA process and also to protect the environmental and public health.
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Clinical biochemical indicators used in diagnostics of equine diseases
Puldová, Doubravka ; Ptáčková, Zuzana (advisor) ; Krejčířová, Romana (referee)
The thesis aims to compile overview of main biochemical indicators that are important in for diagnostics of equine diseases.
Clinical biochemistry is a medical discipline that uses biochemistry and pathobiochemistry for the determination of diagnosis based on activity changes of analytes observed. Laboratory evaluation of analytes has three phases: pre-analytical (preparation of patient to sampling, sampling and sample storage and transport), analytic (analysis) and post-analytical (evaluation of results, veterinarian also contributes in this part). Pre-analytial phase is the most time consuming and also the most error prone. The errors mostly occur during preparation of the patient, during sampling or sample transportation. The most analyzed materials for biochemical analysis are blood, urine and liquor.
Biochemical indicators are compound that are often influenced by the disease, therefore it is possible to use them for the diagnosis. Nitrogen metabolism indicators include proteins, urea, creatinine and ammonia. Enzymes (aminotransferase, gama-glutamyltransferase, glutamatedehydrogenase, lactatedehydrogenase, alkaline phosphatase, creatinkinase and sorbitoldehydrogenase are the main indicators of liver function (they contribute on the metabolism of another compounds) or of the fitness of horse. Glucose and lactate values indicate energetic metabolism, cholesterol and triglycerides indicate lipid metabolism. Water and electrolyte metabolism indicators are sodium, potassium and chlorides. The mineral profile is shown by calcium, magnesium and phosphorus.
The result of determination of analytes is compared with reference values that are not only species-specific but can be also influenced by age or sex. These values represent the range of the compound concentration in body fluid under physiological conditions. It is important to take the clinical symptoms into the account. Not all indicators that are usually indicated in human medicine or different animals are suitable for diagnosis of equine diseases. Majority of indicators diagnose equine-rare diseases (i.e. diabetes mellitus or hepatitis). Determinations of muscle enzymes and lactate are important for the fitness analysis.
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Importance of communication elements for management
Pensdorfová, Tereza ; Fiedler, Jiří (advisor) ; Stanislav, Stanislav (referee)
The diploma thesis Importance of communication elements for management is divided into four main part. The first part is defines aims of the diploma thesis and methodological approach to reach these aims.
The second part is based on theoretical knowledges mainly of management. This part analyse in detail about manager work and especially about interpersonal communication.
The third part contain quantitative research among selected managers and processing primary data. This part provides summarized opinions and experiences of respondent.
The final part compile results of survey. Evaluation is completed by recommendation.
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Analysis of close-to-nature silviculture in the Obora u Kaznějova municipal forests
Blažek, Vojtěch ; Remeš, Jiří (advisor) ; Podrázský, Vilém (referee)
Bachelor thesis describes close-to-nature silviculture at municipal property and its intention is to analyse main silviculture activities practiced here. At the common part there is briefly summarized the historical evolution of close-to-nature silviculture. It also mentions basic nature processes that occur at native forests. The thesis proposes several possible manuals of forest converting. It refers to farming in municipal forests too. In detailed part it analysis farming and nature conditions of the property and it focuses mainly at forest regeneration and stands tending. The results of analysis are being discussed. In conclusion the thesis recommends this type of silviculture mainly for prevention gene pool of forests species.
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Development of two-stroke motocross engines
Přeborovský, Jan ; Miler, Petr (advisor) ; Prikner, Patrik (referee)
The purpose of this thesis is to summarize the development of two-stroke motorcycle engines in the Word. First part is all about history of motocross both in The Czech Republic and the World. This part also includes main milestones of motocross and another sports which are developed from this sport. Second part is devoted to history of two-stroke engines. Third part explains principle of operation of two-stroke engines including description of main parts ant their function. Fourth part counts seven sections. These sections are about development of two-stroke motocross engine from the very beginning to the present, description of main changes in development of these engines. Fifth part evaluates the development of two-stroke motocross engines and considers the next step in the development.
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