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Methods of DNA tandem repeats analysis
Havlík, Kryštof ; Sedlář, Karel (referee) ; Maděránková, Denisa (advisor)
This work clarifies basics of the repetitive DNA and evaluates thee tandem repeats finders, which are accessible to public free of payment. Second part describes an algorithm used for searching tandem repeats, based on converting DNA string into numerical signal. Then follows signal processing using short-time Fourier transform, formation of spectrogram and analysis for evaluating position and content of repetitive areas. Result of this work is comparison of outcomes provided by public accessible programs with results of created program and review of advantages and disadvantages.
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Isolation and characterization of highly repetitive fraction of codling moth, \kur{Cydia pomonella}
VĚCHTOVÁ, Pavlína
Repetitive DNA comprises substantial part of the eukaryotic genome. ?Junk DNA?, as it was originally understood at the beginning of its discovery has attracted a lot of attention lately due to many studies proving its functional perspectives. Analysis of its dynamics, characteristics and distribution has been widely studied in organisms with monocentric chromosomes. Holokinetic system, however, was left behind in these efforts and whole image of repetitive DNA distribution and dynamics in this system remains to be elucidated. In this thesis various approaches were used to isolate and characterise repetitive DNA in the genome of the codling moth, Cydia pomonella. Satellite DNA CPSAT-1 was successfully isolated, characterised with Dot blot and Southern blot and mapped with FISH in the genome of C. pomonella. 17 microsatellite probes were used to localize microsatellite arrays in the genome of C. pomonella. Method of microsatellite FISH revealed distribution of all tested microsatellites in C. pomonella complement.
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