Original title: Isolation and characterization of highly repetitive fraction of codling moth, \kur{Cydia pomonella}
Translated title: Isolation and characterization of highly repetitive fraction of codling moth, \kur{Cydia pomonella}
Authors: VĚCHTOVÁ, Pavlína
Document type: Master’s theses
Year: 2011
Language: cze
Abstract: Repetitive DNA comprises substantial part of the eukaryotic genome. ?Junk DNA?, as it was originally understood at the beginning of its discovery has attracted a lot of attention lately due to many studies proving its functional perspectives. Analysis of its dynamics, characteristics and distribution has been widely studied in organisms with monocentric chromosomes. Holokinetic system, however, was left behind in these efforts and whole image of repetitive DNA distribution and dynamics in this system remains to be elucidated. In this thesis various approaches were used to isolate and characterise repetitive DNA in the genome of the codling moth, Cydia pomonella. Satellite DNA CPSAT-1 was successfully isolated, characterised with Dot blot and Southern blot and mapped with FISH in the genome of C. pomonella. 17 microsatellite probes were used to localize microsatellite arrays in the genome of C. pomonella. Method of microsatellite FISH revealed distribution of all tested microsatellites in C. pomonella complement.
Keywords: codling moth; Cydia pomonella; FISH; GSP-PCR; Lepidoptera; Repetitive DNA
Citation: VĚCHTOVÁ, Pavlína. Isolation and characterization of highly repetitive fraction of codling moth, \kur{Cydia pomonella}. České Budějovice, 2011. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/12831

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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Master’s theses