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Probiotics and prebiotics - a study of interactions, effects and co-encapsulation
Vrtná, Monika ; Němcová, Andrea (referee) ; Márová, Ivana (advisor)
This diploma thesis is focused on encapsulation probiotics and co-encapsulation with some types of prebiotics. In theoretical part is aimed to probiotics, their general characteristics and application of probiotics in food industry. There are described prebiotics and their classification, there is described principles of encapsulation and encapsulation techniques. Methods, which are used for analysis of particles and encapsulation components were introduced too. The experimental part describes methods of prebiotics characterization by high performance liquid chromatography, thin layer chromatography and spectrophotometric methods. Cultivation of probiotics with prebiotics - hydrolyzed and non-hydrolyzed wad tested. Using flow cytometry cell viability was measured too. Finally probiotics and prebiotics were encapsulated, mainly by encapsulator machine. Long-term stability of particles during 6 week storage was observed. The particles were exposed to effect of artificial intestinal, gastric and bile juices.
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Probiotics and prebiotics and their co-encapsulation
Šnajdarová, Karolína ; Bokrová, Jitka (referee) ; Márová, Ivana (advisor)
This bachelor thesis is focused on study of probiotic cultures and options of coencapsulation with specific substrates called prebiotics. Lactobacillus acidophilus and Bifidobacterium breve were used as encapsulated microorganisms. As the specific substrates inulin, psyllium, apple fiber and hemp fiber were tested. Prebiotics was added to probiotics both in untreated form and in hydrolyzed form. The stability and viability of the cells have been studied by microscopy for the period of 6 weeks. Particles were stored in the enviroment of 2.5% citric acid. As the best substrate for cell growth the hemp fiber was found. The hemp fiber exhibited the highest increase of cells count. During observation of long-term stability in the model digestive conditions, particles with hemp fiber were destroyed in instestinal juices. For that reason, particles with hemp fiber could be used in food supplements with targeted transport. The bachalor thesis is also focused on characterisation of prebiotics components. The highest amount of total sugars in the group of non-hydrolyzed prebiotics exhibited psyllium. The highest content of reducing sugars both in the groups of non-hydrolyzed and hydrolyzed prebiotic had hemp fiber. By the method of thin-layer chromatography was found out that the main components of hydrolyzed prebiotics are glucose and fructose. Further, some disaccharides and oligosaccharides were obtained by TLC analysis. For characterisation of prebiotics by HPLC the column Rezex exhibited better sensitivity. HPLC analysis confirmed that the main components of prebiotics are glucose and fructose, some more complex carbohydrates were found too.
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Paraprobiotics, postbiotics and other -biotics
Crha, Richard ; Šálek, Petr (referee) ; Trachtová, Štěpánka (advisor)
The theoretical part of the thesis deals with a wide range of microorganisms and substances derived from probiotics. Based on recent publications, probiotics, prebiotics, synbiotics, paraprobiotics, postbiotics, pharmabiotics, psychobiotics and oncobiotics are defined. Furthermore, the purpose and use of the mentioned -biotics is specified for the current understanding of their function. In the experimental part, DNA was isolated from a sample of probiotic capsules using a commercially available bacterial kit and magnetic particles. Its purity and concentration were then determined spectrophotometrically. Using the isolated DNA as a matrix for conventional PCR and detection on agarose gel, the composition of the probiotic product as declared by the manufacturer was demonstrated. Finally, the PMA-PCR method was optimized.
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Probiotics, prebiotics, synbiotics and postbiotics in breast milk and formulas.
ŠEBÍKOVÁ, Lucie
Intestinal microbiotics make up about 80 % of our immunity. The biggest colonization by these microorganisms happens in the first 1000 days in the life of a child, which is why it is important to maintain a balanced diet, to properly support growth and diversity of an intestinal flora. In the theoretic part of my thesis I will look closely on composition of breast milk and on other kinds of substitution of dairy nutrition. You will learn that many factors affect the composition of intestinal microbiotics. For if it comes to dysmicrobia, for example as a result of long-term use of antibiotics, it could lead to development of metabolic, immunologic and psychic issues. The goal of my bachelor thesis was to learn the contents of probiotics, prebiotics, postbiotics and synbiotics in breast milk, and in the formulas in the 1st, 6th, 12th and 18th month. My sources were professional literature and the websites of chosen dairy nutrition. For learning the exact intake of the observed substances I evaluated menus from 10 respondents. Breast milk is the most natural form of nutrition, which is why it is used as a template for making the substitution of dairy nutrition. The closest to breast milk in contents of synbiotics are brands Hipp BIO combiotik and BEBA COMFORT HM-O. Nutrilon advance has the most similar count of prebiotics, which was confirmed by observing the specific intake from menus. Nutrilon and Hami are only brands containing postbiotics thanks to the process of directed fermentation Lactofidus. This data should help in deciding what substitution of dairy nutrition to choose, if a child cannot be breastfed. But since this thesis does not evaluate the complete contents of the formulas, but rather the contains of the observed substances, we cannot presume the brands with the best results are the best option of nutrition. The choice of nutrition should be discussed with a pediatrician or a nutritionist.
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Feed additives in pig nutrition
KOZINOVÁ, Eliška
Nutrition is one of the most important aspects of pig farming. Each category of pigs is fed by a special compound feed for a given group of animals. New alternatives to these drugs are still being explored due to the 2006 ban on growth-promoting antibiotics. Probiotics, prebiotics and phytobiotics, which have a positive effect on the digestive system of all categories of pigs, are becoming very popular substitutes.
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Level of awareness of university students about the impact of probiotics on human health
ŠTROBLOVÁ, Petra
Probiotics are your microorganisms that, when properly dosed, support the development and function of microbiots that affect their health. They currently create affordable, biological methods in the prevention and treatment of personal illness. They support the physiological function of the gastrointestinal system, which forms the body's main immune system. The bachelor thesis was focused on the possibilities of using probiotics and their effect on human health. The aim of the bachelor's thesis was to compile a questionnaire survey and its request for information of university students in the field of probiotics. The survey found that the awareness of university students is very good, it can be a problem in this problem.
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Probiotics and their importance in terms of human health
ŠTROBLOVÁ, Petra
Probiotics are living microorganisms that, when properly dosed, support the development and function of an individual's microbiota, and thus overall health. The aim of this bachelor thesis was to assess the public's awareness of probiotics through a questionnaire survey and to evaluate the offer of probiotic preparations on our market. Of the 551 respondents, the majority (63 %) knew what probiotics are, what effects they have (96 %) and in which food they are most abundant (81 %). The research of the probiotic market found that the offer of these supplements in our market is very wide. A total of 72 synbiotic and 12 probiotic food supplements from 41 firefighters are available.
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Využití probiotických krmných aditiv ve výživě telat
POLÍVKOVÁ, Denisa
The aim of this study was a brief elaboration of a literature review containing the morphology of the digestive system of calves, the issue of methods of their breeding and feeding, probiotics, prebiotics and phytobotics. The following study aims to confirm the positive effect of probiotic feed additives containing the probiotic strain Lactobacillus Sporogenes on calves. A total of 80 calves were included in the project, divided in half into two groups, control and experimental. Both groups were weighed three times, within two hours after birth and then according to the individuality of the individuals at shifts of an average of 56 and 100 days of age. During breeding, in addition to weight, the general state of health, the frequency of diarrheal diseases. From the evaluation of the collected data, it can be stated that the feeding of probiotic additives containing the Lactobacillus Sporogenes strain has a significant effect not only on the health status but also on the weight gain of calves.
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Význam aditivních látek ve výživě drůbeže
STUNOVÁ, Tereza
Bachelor thesis is about differences in morphology and physiology of poultry as affected by nutrition, with special focus on effects of nutritional additives. The thesis focuses mainly on a group of zootechnical additives. Description of these additives and their effect on poultry is discussed.
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Addition of probiotics to baby food products
Dudrová, Markéta ; Skoumalová, Petra (referee) ; Márová, Ivana (advisor)
This Diploma thesis deals with preparation of probiotic cultures Lactobacillus acidophilus, Lactobacillus casei and Bifidobacterium breve enriched with prebiotics meant for application in baby food products. Natural extracts from matcha, moringa, young beat, young barley, chlorella and spirulina were selected as prebiotics. The theoretical part is focused on probiotic bacteria, their biological effects and their effects on the child´s body. The experimental part deals with the cultivation of probiotic bacteria with plant extracts, monitoring their viability and stabilization in an encapsulated form. Mixtures of probiotic cells with prebiotics were encapsulated into alginate particles to increase stability. Some of the alginate particles were processed by freeze drying. Mixtures of probiotic cultures with plant extracts were subjected to model human digestion by the action of model digestive juices in unencapsulated, encapsulated and lyophilized form. Selected extracts of plant materials were characterized in terms of amount of total and reducing sugars, total phenolic substances, individual phenolic substances and antioxidant activity. Further, two baby commercial dietary supplements containing probiotics were selected, which were characterized in terms of cell number and viability. Probiotic products were also subjected to model digestion.
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