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Feed additives in pig nutrition
KOZINOVÁ, Eliška
Nutrition is one of the most important aspects of pig farming. Each category of pigs is fed by a special compound feed for a given group of animals. New alternatives to these drugs are still being explored due to the 2006 ban on growth-promoting antibiotics. Probiotics, prebiotics and phytobiotics, which have a positive effect on the digestive system of all categories of pigs, are becoming very popular substitutes.
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Level of awareness of university students about the impact of probiotics on human health
ŠTROBLOVÁ, Petra
Probiotics are your microorganisms that, when properly dosed, support the development and function of microbiots that affect their health. They currently create affordable, biological methods in the prevention and treatment of personal illness. They support the physiological function of the gastrointestinal system, which forms the body's main immune system. The bachelor thesis was focused on the possibilities of using probiotics and their effect on human health. The aim of the bachelor's thesis was to compile a questionnaire survey and its request for information of university students in the field of probiotics. The survey found that the awareness of university students is very good, it can be a problem in this problem.
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Probiotics and their importance in terms of human health
ŠTROBLOVÁ, Petra
Probiotics are living microorganisms that, when properly dosed, support the development and function of an individual's microbiota, and thus overall health. The aim of this bachelor thesis was to assess the public's awareness of probiotics through a questionnaire survey and to evaluate the offer of probiotic preparations on our market. Of the 551 respondents, the majority (63 %) knew what probiotics are, what effects they have (96 %) and in which food they are most abundant (81 %). The research of the probiotic market found that the offer of these supplements in our market is very wide. A total of 72 synbiotic and 12 probiotic food supplements from 41 firefighters are available.
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Využití probiotických krmných aditiv ve výživě telat
POLÍVKOVÁ, Denisa
The aim of this study was a brief elaboration of a literature review containing the morphology of the digestive system of calves, the issue of methods of their breeding and feeding, probiotics, prebiotics and phytobotics. The following study aims to confirm the positive effect of probiotic feed additives containing the probiotic strain Lactobacillus Sporogenes on calves. A total of 80 calves were included in the project, divided in half into two groups, control and experimental. Both groups were weighed three times, within two hours after birth and then according to the individuality of the individuals at shifts of an average of 56 and 100 days of age. During breeding, in addition to weight, the general state of health, the frequency of diarrheal diseases. From the evaluation of the collected data, it can be stated that the feeding of probiotic additives containing the Lactobacillus Sporogenes strain has a significant effect not only on the health status but also on the weight gain of calves.
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Význam aditivních látek ve výživě drůbeže
STUNOVÁ, Tereza
Bachelor thesis is about differences in morphology and physiology of poultry as affected by nutrition, with special focus on effects of nutritional additives. The thesis focuses mainly on a group of zootechnical additives. Description of these additives and their effect on poultry is discussed.
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Addition of probiotics to baby food products
Dudrová, Markéta ; Skoumalová, Petra (referee) ; Márová, Ivana (advisor)
This Diploma thesis deals with preparation of probiotic cultures Lactobacillus acidophilus, Lactobacillus casei and Bifidobacterium breve enriched with prebiotics meant for application in baby food products. Natural extracts from matcha, moringa, young beat, young barley, chlorella and spirulina were selected as prebiotics. The theoretical part is focused on probiotic bacteria, their biological effects and their effects on the child´s body. The experimental part deals with the cultivation of probiotic bacteria with plant extracts, monitoring their viability and stabilization in an encapsulated form. Mixtures of probiotic cells with prebiotics were encapsulated into alginate particles to increase stability. Some of the alginate particles were processed by freeze drying. Mixtures of probiotic cultures with plant extracts were subjected to model human digestion by the action of model digestive juices in unencapsulated, encapsulated and lyophilized form. Selected extracts of plant materials were characterized in terms of amount of total and reducing sugars, total phenolic substances, individual phenolic substances and antioxidant activity. Further, two baby commercial dietary supplements containing probiotics were selected, which were characterized in terms of cell number and viability. Probiotic products were also subjected to model digestion.
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Probiotics, their influence on health and probiotic food
Hondlíková, Tereza ; Dostálová, Jana (advisor) ; Plocková, Milada (referee)
Probiotics are living microorganisms which show positive effects on human health if consumed in a sufficient quantity. Probiotics have been under a lot of research recently, however their effectiveness in terms of treatment or prevention of diseases has not been supported by a sufficient number of studies and research yet. The thesis summarizes the existing knowledge about probiotics, characterizes individual types of probiotic organisms, describes possible sources of probiotics and their impact on human health. Theoretical part of the thesis deals with the definition of the concept of probiotics, also describes prebiotics and synbiotics which are closely related to this topic. characteristics of individual groups of probiotics as well as their impact on human health and possible use in the treatment of diseases as a form of biological treatment are also included. Furthermore the sources of probiotics both in the form of foods containing probiotics and in the form of probiotic dietary supplements are described. At the end of the theoretical part, recommendations for receiving and selecting a suitable source of probiotics are summarized. The aim of the practical part was to find out if the general public is aware of probiotics. Questions about preferences in the selection and consumption of...
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Biofilm formation in probiotic cultures and its application in pharmacy
Ryšávka, Petr ; Obruča, Stanislav (referee) ; Vorlová, Lenka (referee) ; Márová, Ivana (advisor)
The work was comprehensively focused on the development of adhesive forms of probiotics in the form of a biofilm on combined carriers with a prebiotic component. The second part dealed with the influence of food on the multiplication and survival of selected types of probiotic bacteria. Subsequently, the effect of individualized probiotic supplements on changes in the human intestinal microbiome was monitored. Suitable adherent probiotic strains for biofilm formation were selected and tested. Methods have been introduced and different variants of carriers for culturing and binding bacteria have been tested. In vitro experiments verified the stability of biofilm stucture and its resistance to low pH, bile and antibiotics in comparison with the planktonic cell form. The antimicrobial effect of probiotic strains in the form of a biofilm was studied. The cultivation of the multispecies biofilm on the combined carrier was optimized and the stability of the biofilm and the final viability of probiotic bacteria were confirmed. Furthermore, the influence of various foods and beverages on the viability of probiotic bacteria was evaluated with emphasis on the simulation of passage through the gastrointestinal tract. Both models, solutions with standardised concentrations of alcohol, sugar, salts, proteins or different pH and different types of real foods and beverages were tested. The effect of food and beverages was tested on monocultures of Lactobacillus acidophilus, Bifidobacterium breve and on probiotic capsules containing a mixed culture of probiotic microorganisms. The survival of probiotics in various food matrices in the simulated gastrointestinal tract was quantitatively different. We managed to define foods suitable for supporting the multiplication of probiotic bacteria. A separate part of the work was focused on the targeted modulation of the intestinal microbiome by individualized probiotics that were prepared on the basis of molecular biological analyzes of the intestinal microbiome aimed at detecting the percentage of lactobacilli, bifidobacteria and phylum Firmicutes and Bacteroidetes. Personalized probiotic supplementation confirmed the positive effect of this approach on microbiome changes, especially on the increase of the content of lactobacilli, bifidobacteria and the overall diversity of the microbiome.
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Changes in microbial colonization of gastrointestinal tract in patients after allogeneic hematopoietic stem cell transplantation
Michková, Petra ; Mrázek, Jakub (advisor) ; Kodešová, Tereza (referee)
Introduction: Physiological microflora is characterised by wide diversity. The microbial community is mostly composed of bacteria, but also includes fungi, archaea and viruses. Anaerobic commensal bacteria (Firmicutes and Bacteroidetes) dominate 90% of the colon. The composition and products of the gut microbiota have a significant effect on an individual's immune system, and their interactions may ultimately promote immune tolerance or inflammatory immune response. Blood cell transplantation (HSCT) and its associated standard procedures of conditioning, antibiotic exposure and dietary prophylaxis represent modification and disruption of the gut microbiota leading to the development of some serious post-transplant complications affecting the OS (overall survival) and TRM (treatment related mortality) of patients. Objectives: The aim of this work was to investigate the representation of individual bacterial strains in patients undergoing allogeneic HSCT, as well as the effect of transplantation on the composition and diversity of their gut microbiota. Methods: Stool samples were obtained from 52 patients who underwent an allogeneic hematopoietic cell transplant at the Institute of Hematology and Blood Transfusion in Prague. A cut-off date for the first sample was set for the start of...
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Probiotics, prebiotics, synbiotics - are they really effective?
Šmídová, Zuzana ; Pejšová, Hana (advisor) ; Pechar, Radko (referee)
Probiotics and prebiotics constitute an important part of our nutrition and possess many positive effects on human organism. Synbiotics represent combination of both of them. The aim of the theses in the theoretical aspect was to create a systematic review of available knowledge on the given topics and in the practical part to assess the level of awareness on these topics with regard to their presence and effects. In the practical part of the theses the questionnaire survey methodology was used. After evaluation of all answers it was found out that most people are familiar with the term probiotics, less people with the term prebiotics and the least respondents with the term synbiotics. Fermented dairy products are consumed by 88,3 % of respondents, mostly 2-3 times a week. The rest not consuming them indicated mostly the reasons of bad flavour or some form of intolerance. The respondents know also other fermented foods (sauerkraut, vegetables), however, these are less consumed than fermented dairy products. The least often the food supplements containing these substances are consumed. Due to the low level of awareness on prebiotics and synbiotics it would be very appropriate to improve public awareness on the given topics in order to increase their consumption, mainly in the form of their natural...
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