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Influence of age and rearing conditions of a fish on the chance to survive in the wild
Lyach, Roman ; Frouzová, Jaroslava (advisor) ; Čech, Martin (referee)
The task of this thesis is to summarize all the most important biotic and abiotic factors that can influence chances of reared fish to survive in the wild. It contains some of the most frequently reared and popular fishes, such as Sander lucioperca (pike perch), Samo trutta (trout), Micropterus salmoides (largemouth bass), Esox lucius (northern pike), Esox masquinongy (muskellunge) and the hybrid of northern pike and muskellunge, the tiger muskellunge. Mainly the factors such as success, survival, rate of growth and mortality have been studied. Survival increases with the size of the fish because of the fact that larger fish can easily avoid predation. Pellet-reared fish show higher mortality than minnow-reared fish because of the loss due to predation and also slightly worse ability to catch living prey and obtain food, even though their diet contains similar species. Temperature can also affect the mortality of the stocked fish, mainly when the temperature difference between the rearing pond or hatchery and the new environment exceeds 10 řC, which brings loss of majority of the stocked fish. It has been revealed that genetics is very important during the process of stocking, while fish from the local population show better abilities to adapt to the environment than those from other geographic...
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The Evolution of Jordanian Political Regime After 1989
Ducháčková, Michaela ; Buben, Radek (advisor) ; Koubek, Jiří (referee)
The aim of this master thesis is to analyze political regime of the Hashemite Kingdom of Jordan and to find out some possible effects, which could have had an influence on its stability and survival in the last 25 years. The Jordanian political regime had gone through several crises in the examined years. Not only did it survive all of them but also became stronger. Which factors caused the survival of the regime? In the first part of the thesis we typologize the regime from three possible perspectives (institutional, formalistic and legitimation). The second part deals with an analysis of factors, which enable the survival of Jordanian political regime. The emphasis is given on the role of institutions.
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Hypercapnia and diffusion of gases in avalanche (Changes in functional parameters of individuals in a crisis situation)
Mašek, Michal ; Bartůňková, Staša (advisor) ; Roubík, Karel (referee) ; Rotman, Ivan (referee)
Název: Hyperkapnie a difuse plynů ve sněhové lavině - (Změny funkčních parametrů jedinců v krizové situaci) Cíle práce: Cílem studie bylo kontinuální sledování kompenzačních mechanismů, zejména změn ventilačně-respiračních ukazatelů, v simulované sněhové lavině. Metoda: K získání dat byl použit experiment a nestandardizovaný dotazník. Výběr souboru byl vzhledem ke sledované problematice přísně selektivní. Výzkumu se účastnilo 22 probandů, ale po analýze dat bylo pro nesplnění požadovaných kritérií 11 osob vyřazeno. Soubor tedy tvořilo 11 zdravých mužů průměrného věku 25,3 let. Před vlastním experimentem byla pomocí osobního spirometru testována senzitivita na hyperkapnii a hypoxii (výdrž v apnoi) a dechová zdatnost (vitální kapacita plic s usilovným výdechem). Experimentální situaci představovalo jak dýchání do uzavřeného objemu (8 l), tak do vytvořené vzduchové kapsy ve sněhu (400 ml). Kontinuální záznamy oběhových funkcí (srdeční frekvence, krevní tlak) a ventilačně-respiračních parametrů (dechová frekvence, dechový objem, minutová ventilace, obsah O2 a CO2 ve vdechovaném a vydechovaném vzduchu, odpor při výdechu a saturace krve kyslíkem) byly snímány pacientským monitorem DATEX Ohmeda. Vzhledem k charakteru dat byla využita analýza rozptylu při opakovaném měření se dvěma faktory (dvoucestná...
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Not So Close That Breck
Smrekovský, Adam ; Jančík, Alexandr (referee) ; Šrámek, Jan (advisor)
How to stop working, and start catching dogs, and live alone on a hill and walk down only when you need to catch a dog. But it also stops entertaining you and you start thinking about village life. And so you run away from the end of the world to the village.
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Usage of formylmethionin derivates in tumor immunotherapy (Model melanoma B16-F10)
VLČKOVÁ, Zuzana
Cancerous diseases and alternatives of their therapy belong to one of the most discussed issues of present day. Cancer is one of the most common lifestyle diseases. It is estimated that external factors cause the origin of 75 % of all cancerous diseases. The most influential external factors are hectic lifestyle, obesity, wrong eating habits, smoking, excess of alcohol intake, inappropriate sexual behaviour and negative approach to preventive check-ups and at last the permanently increasing lifespan of the population. The most serious problem is the occurrence of malignant tumors that are diagnosed so late. Therefore, the aggressive therapy is often the choice of treatment. But this kind of therapy often irretrievably damage the health of the patient. Modern medicine tries to find some new ways of cancer therapy. One of them is treatment of tumors by targeted immunotherapy using the naturally occurring matters. The main aim of this bachelor thesis is to describe and verify the possibility of treatment of malignant melanoma by formylmethionin derivates. The bachelor theses consists of two parts. Theoretical part is focused on describing the issue of cancerous diseases, tumor formation and classification. It focuses on tumors of skin, their diagnostics and possibilities of the therapy, especially of the immunotherapy by innate immunity activation. In experimental part of thesis is described the cultivation of melanoma cell line B16-F10 for in vitro experiments and the transplantation of cells into syngeneic mice strain C57BL/6 from Charles River Laboratories. Melanoma cells B16-F10 in the number of 400 000 cells were transplanted to 8 weeks old mice, which had a weight of 18 - 20 grams. The methods of measuring tumor size and comparing tumor temperature versus mouse body temperature is also described. The result of the first experiment concludes, that therapy of B16--F10 tumor by f-MLF-(K)12 motif bound by charge do not work, because it does not cause phagocytic attack. In second experiment it was found out that B16-F10 tumor therapy by 3mM f-MLF alone does not work, because it does not cause tumor reduction without the LPS in combination. In the third experiment f-MLF motif was used. It was anchored by lipophilic chain with stearic acid, it was used alone or in combination with LPS. In this experiment the complete temporary elimination of tumors occurred, but after therapy termination the tumor growth appeared almost immediately. The mouse treated by this compound survive for more than 52 days than control mice. Due to experiments performed in this bachelor thesis was found out, that anchoring of formylmethionin peptides to tumor cell have positive effect to decrease of tumor volume in successful immunotherapy.
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Intenzivní chov okounka pstruhového (Micropterus salmoides) v RAS s různým světelným režimem
BOŇKO, Dominik
Largemouth bass (Micropterus salmoides) is one of the species of fish that don´t have a significant position among the species of fish breeded in Czech Republic. However the trend of building aquaculture recirculation systems with warm water comes to the fore. Breeding in these conditions opens up the possibility of introducing new kinds of thermophilic fish species. Largemouth bass definitely belongs to this group. The general factors of successful breeding of largemouth bass are the temperature and purity of the water, the suitable-composed diet and the correct stocking density. This work studies another factor that not only participates in the success of the breeding, but also has influence on the energy expenses of the aquaculture system. It is the effect of photoperiod during breeding. Five experimental groups with three repetitions were created according to the time of lighting (8, 12, 16, 20 and 24 hours of light a day). The experiment lasted 16 weeks. The average water temperature was 24,6 °C. The initial stocking density for all tanks was 20.6 kg . m-3. Fish had an initial average weight of 32.6 +- 2.1 grams. The feed ration was set up at 1% of biomass per day. As a diet were used a floating dry pellets Skretting R-2 Europa 15 F of 2 mm. The feed contained 55 % of protein and 16 % of fat. Fish were fed manually by hand. All groups showed high survival during the experiment (99.4 - 100 %). The best results were achieved by a group of 8 hours with the highest feed conversion (FCR = 0.94 +- 0.06) and the highest growth rate (SWGR = 0.6 +- 0.04 % . day-1). The average individual fish weight of the 8 hour group at the end of the experiment was 64.07 +- 2.82 g. On the other hand, group of 12 hours was the worst with the lowest average individual weight (W= 57.51 +- 1.51 g), growth rate (SWGR = 0.49 +- 0.04 % . day-1) and the lowest feed conversion (FCR = 1.16 +- 0.08). These two groups were significantly different in most production indicators. The pervisceral fat index grew with biomass. The longer the photoperiod, the higher the plasma and ammonia content of lactate. In contrast, glucose content decreased. A longer photoperiod also influenced the higher production of antioxidants.
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Vliv teploty na udržení schopnosti oplození a líhnivosti při přechovávání neoplozených jiker u lína obecného
ANDONIU, Andreas
This theses deals with the storage length of artificially spawn of hard roes of Tench (Tinca tinca) during different temperatures at the time before the semen discharging and activation to fertilization, hatching and consequent survival of fish hatchery throughout changeover from the embryonic to larval life period (beginning of active food intake). Homogeneous assortment of hard roes obtained from hormonally induced artificial hatching of 6 spawners has been used for this experiment. Samples of hard roes were put into plastic bowls and covered, immediately after artificial hatching. Subsequently, they were placed into tempered, thermo-isolating containers with temperatures of 5, 10, 15, 20, 25 and 30 °C. In time intervals of 0.5; 1; 1,5; 2; 3; 4; 6; 8 and 10 hours, a small amount of hard roes were taken away from each temperature (estimated 50 -100 pieces) and put into dry, glass beakers (in 3 repetitions in each temperature combination and length of storage). Subsequently, the semen discharging from 6 milters was carried out and activation by water was performed. Incubation took place in non-sticking environment. During the incubation, or more precisely during the consequent storage of embryos through temperatures between 19-20.5 °C, water was changed daily. Fertilization was evaluated 48 hours after fertilizing. Hatchery was determined 48 hours after beginning of hatching of first specimen. After changeover from embryonic to larval period of ontogenetic development, living food was offered to hatching fish (artemia sp.). Thereafter, the amount of hatched fish with filled intestines was counted. Ascertained values were depicted as a percentage from the total number of seeded hard roes as well as fertilized hard roes with the use of statistic methods (two factors Anovy with the repetition). The highest level (in statistic evaluation on the importance level alfa = 0.05) of hard roe hatchery was accomplished throughout the length of possession and temperature 1 hour/ 25 °C (68.0 +- 3.1 %). The high level of hatchery was maintained by hard roes stored for 2 hours, afterwards a gradual value decrease was registered. Similarly, that was achieved with hatching parameter, where the high level of hatching was achieved with hard roes possessed for the period of 3 hours (except temperature of 30 °C), afterwards the hatchery was decreased. Pursued survival and food intake parameters of hatched fish (from the practical point of view) confirmed above stated dispositions. The high hatchery from placed hard roes was maintained for 1.5 - 3 hours (except 30 °C), thereafter there was its gradual decrease. In the time of 8 hours (temperatures 5 - 20 °C), the survival of 1.2 +- 1.8 %, was found out, with the rest, the survival was nearly zero.
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Selected aspects of intensively cultured European whitefish (Coregonus maraena, Bloch) and peled (Coregonus peled, Gmelin)
ŠEBESTA, Roman
Maraena whitefish and peled are considered to be economically important fish species. This Ph.D. thesis deals with testing of selected aspects which show potential for improvement of quality and effectivity of larvae and juveniles of both fish species in recirculating aquaculture systems. The whole Ph.D. thesis includes 9 chapters described in 125 pages. In chapters 2-8, there are described the individual factors which can influence quality of intensive rearing of mentioned fish species. The effect of light intensity and tank wall colour on growth and survival of peled larvae is tested in chapter 2. The effect of different temperature on growth and survival of maraena whitefish larvae is presented in chapter 3. The effect of various stocking density on growth and survival of maraena whitefish larvae is given in chapter 4. The influence of different feeding strategy on survival, growth, and intestine and liver development of maraena whitefish larvae is being shown in chapter 5. The effect of various weaning time and co-feeding duration on growth and survival of peled larvae is described in chapter 6. The effect of variable water oxygen saturation on growth and haematological profile of peled juveniles is shown in chapter 7. The prevalence of deformities in intensively reared peled and comparative morphometry in intensively reared fish compared to pond-reared fish is described in chapter 8. Ph.D. thesis contains important advices which can be used in fishery practise and at the same time they can prevent from elevated fish mortality.
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