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Methodology for detecting the presence of cranberries and lingonberries in processed products using molecular SSR markers: methodology for practice
Kučera, Ladislav ; Ovesná, Jaroslava
The subject of the methodology is a procedure for diagnosing the presence of lingonberries (Vaccinium vitis-idaea L.) and cranberries (Vaccinum macrocarpon L.) in processed products such as tea or fruit ingredients of many food products (jams, jelly, purree, tea). The methodology exploit DNA sequences that are typical for each plant species. Thus, molecular DNA markers that characterize the plant species by SSRs (single sequence repeats) are used. The methodology describes the extraction of DNA from the assumed matrix, the use of selected SSR markers and the analytical data evaluation procedure. The method can be used in any solid equipped molecular-genetic laboratory. The method is applicable for the characterization of DNA isolated from any part of the plant, fruit, and derived food or food supplements. Method describes the procedure and necessary equipment to perform the analysis. Protocol recommends how to evaluate the results.
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Chosen problems of genetic diversity of dog
CHLUPATÁ, Lucie
The bachelor thesis is worked out in the form of the research on the theme "Selected questions from the genetic diversity of dogs". The aim of this work was processing of the current situation of scientific knowledge in the field of genetic diversity of dogs. Firstly, the origin is described in the thesis, domestication of dogs, an organisation dealing with dogs breeding and selected breeds of dogs. From the genetic site point of view, the basic terms in genetics are described genetic markers, DNA profile of dog and the methods of evaluation of genetic diversity. In the end of my bachelor thesis I outlined possible recommendations for dog breeders.
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Genetic diversity of cattle in Czech Republic
ČERVINKA, Václav
The thesis deals with genetic diversity in the Czech Republic. Firstly domestication of livestock in general and then domestication of cattle is decribed. History of cattle in the Czech Republic, the importance of cattle breeding and last but not least the condition of cattle in the Czech Republic is also introduced. The thesis is also focused on biodiversity, threats to biodiversity and its conservation and the levels of diversity are also specified. Genetic markers,microsatellites, minisatellites, SNP, polymorphic structural genes and also mutations are described. The cattle breeds farmed in the Czech Republic are specified next. The National Programme, genetic resources and genetic resources in the Czech Republic are evaluated. Finally genetic resources of " česká červinka" and "český strakatý skot" are described.
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Genetic diversity of chosen horse breeds
JANKOVSKÁ, Tereza
The objective of the thesis is to clarify the findings about genetical biodiversity of horses, evaluation of the diversity and description of current state of genetic resources preservation. Firstly, the history is described, domestication of horses, breeds and biodiversity. From the genetic point of view, the thesis contains description of the genetical diversity, microsatellites, markers and mitochondria. There are described genetic sources and their preservation. Current quantities and thein progress are also mentioned.
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Edited final report of project QH81052
Patzak, Josef ; Matoušek, Jaroslav
Cílem projektu bylo vyvinout molekulárně-genetické markery odvozené od genetických elementů a sekvencí genů kodeterminujících metabolom chmele. Zdrojem specifických nových sekvencí byly expresní knihovny hlávek a lupulinových žlázek chmele, genomová a BAC knihovna, které byly získány nebo připraveny v rámci řešení projektu.
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