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The application of microorganisms to the degradation of lipids
Vaňásek, Jakub ; Voběrková, Stanislava (referee) ; Omelková, Jiřina (advisor)
This bachelor thesis is focused on the study of commercial products for degradation of lipids in waste water. This commercial products contain microorganisms with lipolytic activity. Theoretical part of this thesis describes lipids, lipases, mikroorganisms which produce lipolytic enzymes and basical principles of cleaning of waste water. In this part analytical methods of lipids isolation are presented too. The practical part of this thesis is concerned with the study of three commercial products. These products were tested for the content of microflora. Subsequently the restriction analysis was performed. Using this method the microorganisms were identified as Bacillus sp. Furthermore the growth curves were determined. This curves show growth of biomass. Then the pH during cultivation and lipolytic activity of microorganisms were determined. Lipolytic activity was determined by spectrofotometric method with using of p-nitrophenyllaurate.
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Microorganisms with lipoplytic activity and their applications
Pavlačková, Jana ; Omelka, Ladislav (referee) ; Omelková, Jiřina (advisor)
Lipases are hydrolytic enzymes that are produced by many types of microorganisms. This thesis describes not only the microorganisms which produce lipases but also different possibilities for the industrial utilization of lipases. Lipases are widely used in reactions where the combination of a lipophilic substrate with a hydrophilic one is necessary – in the synthesis of ascorbic acid fatty esters, sugar esters, lipoaminoacids and in the lipophilization of phenolic derivatives. Lipases are also important in relation to environmental protection. For example, they are used for the purification of waste water. In this thesis, five different preparations containing microorganisms with lipolytic activity were tested for lipolytic activity. There are many ways of determining lipolytic activity. The spectrophotometric determination of lipolytic activity that uses the ability of lipases to divide p-nitrophenyllaurate to p-nitrophenol may serve as an example. After this p-nitophenol is detected spectrophotometricaly.
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