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Možnosti obohacení pekařských výrobků luštěninovými moukami
Lisá, Andrea
The bachelor thesis engages legumes in general, from their cultivation to their final processing. I focused on analysis of the chemical composition of the legume seeds and on the effect of the antinutritional factors on the contained nutrients. I worked in more detail on the use of the legumes for flour, on its use in bakery and on its influence on the resulting bakery products. Legume flours increase in the bakery products content of proteins, lipids, fiber and minerals and they decrease content of carbohydrates and gluten. They increase absorption of water and the development time of the dough. The stability of the dough is affected negatively. The stability is also prolongated by adding 10% of chickpea flour. Lentil flour increases water absorption and the development time of the dough since the addition of 50 % of flour. Final bakery products are less cambered, with the characteristic darker colour of the crust and with the characteristic taste. Legume flours are also often added into the dough of the cookies. Soya flour is even added into the donut dough to reduce the absorption of the fats during frying.
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Ecology of critically endangered \kur{Sedum villosum}-experimental study
DILLINGEROVÁ, Hana
The aim of this diploma thesis was to elaborate a literature survey and especially to conduct an experimental study of ecological demands of the critically endangered species Sedum villosum. The localities, on which S. villosum formely occurred, disappeared largely because of habitat destruction by draining and plowing of wet meadows, or by the abandonment followed by development of high vegetation and biomass accumulation. Therefore, this work could contribute to the knowledge of shadow influence and surrounding vegetation concurency on seed germination and flowering of this species. Retrieved knowledge can be later used for rescue program realization (repatriation options, strengthening populations, habitat requirements). The experiments showed a significant negative effect of the shading and the presence of surrounding vegetation (sedges, brown mosses and peat mosses) on germination and flowering of S. villosum. Furthermore, it was found that S. villosum is able to germinate directly on water surface, with seed germination even higher than on wet soil. The experimental sowing, planting and subsequent monitoring of germination, growth and survival of S. villosum at the rehabilitated area of Knížecí Pláně (NP Šumava) showed that it is possible to strengthen S. villosum population by sowing and planting autochthonous material on prepared areas with a torn down turf. As an acceptable tools seems to be using mulch from mown in the area of the original population. Similar process can be used in the future for restoring historical localities of this species.
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Evaluation of modifications in content of selected bioactive substances in flax (\kur{Linum usitatissimum} L.) seeds during germination
HAŠKOVÁ, Kateřina
This thesis focus on analysing effect of germination on some groups of bioactive compounds in flaxseed. We used flaxseed variety named Libra. Its germination was 10 days long, done in two different conditions: in the dark and fully iluminated for 24 hours daily. Samples were taken on 2nd, 4th, 6th, 8th and 10th day. Following analyses were performed: determination of dry matter content, lipid content, content of nitrogenous compounds, content of proteins and protein spectrum, chlorophyll content, content of total polyphenolic compounds and antioxidant activity. Content of dry mater was proved to decrease during germination as well as lipid content regardless of light conditions. Amount of nitrogenous compounds stayed relatively unchanged during germination. Whereas amount of proteins increased depending on duration of germination and light conditions. Hydrolysis of proteins during germination was proved by protein spectrum analysis. Content of chlorophyll inreased in light-germinated seeds only. Increasing effect of germination on total polyphenolic compounds content and antioxidant activity was proven as well. Moreover aproximately two-fold rise of values was reported after defatting of samples.
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Mechanisms that control physiological seed dormancy
Řezková, Natálie ; Ponert, Jan (advisor) ; Vosolsobě, Stanislav (referee)
Physiological dormancy is an important developmental trait ensuring that seed does not germinate when the environmental factors are appropriate only temporary. The transition from seed dormancy to germination is regulated by a large number of factors and the phytohormone abscisic acid (ABA) plays a crucial role. Enhanced response to ABA and its biosynthesis is a key mechanism in dormancy induction and maintenance. ABA interacts antagonistically with gibberellins (GAs). Therefore GA biosynthesis and ABA catabolism are positive germination regulators. However, other phytohormones are also involved in the regulation of dormancy and germination. The most studied is ethylene which supports germination similarly to GA. Numerous factors affect dormancy at molecular level, namely chromatin remodeling, gene products that function only in dormancy regulation [e.g. DELAY OF GERMINATION 1 (DOG1)] or gene products that mediate seed response to environmental factors. The dormancy, its induction, depth and release, is driven not only by environmental conditions affecting mature seeds, but also by conditions acting during seed maturation in a maternal plant when the primary dormancy is induced. Requirements for dormancy release and germination induction may vary considerably between species. The physiological...
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Effect of climate on germination and selection of different genotypes
Duongová, Thuy Lien ; Münzbergová, Zuzana (advisor) ; Dostál, Petr (referee)
Understanding the response of species to climate change and their ability to adapt is the key to describe the future development of plant communities. The aim of the study is to determinate intraspecific variability in germination of Festuca rubra from different original climates in response to novel climatic regimes. This study also observes if different climatic regimes lead to selection of different genotypes. Festuca rubra is a widespread clonal grass occurring in the Northern hemisphere. The plant material comes from 11 localities distributed along a climatic grid of factorially crossed temperature and precipitation situated in western Norway. The project was carried out in growth chambers, where the germination of seeds was monitored in two different temperature conditions and in two moisture treatments. Germinated seeds were planted into pots remaining in the same treatment where they germinated. Seedlings from one Petri dish grew together in one pot. One population, from the coldest and the driest original locality, growing in the warm-wet and cold-wet treatments was genetically analysed using microsatellites. Germination of the species was higher and faster in warm than in cold conditions, showing that germination of the species is enhanced by higher temperature. Germination was higher in...
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Seed germination and seedling recruitment of Iris sibirica depending on the type of management
HOŘEJŠÍ, Edita
This work continue on a bachelor's work which investigated production characteristics of the plant species Siberian Iris (Iris sibirica). The aims of the presented work are reproductive properties by seeds of Siberian Iris in relation to the seed germination and with various meadow managements. Seeds of Siberian Iris (collected in 2015 and 2016) were investigated in laboratory condition and at meadow near the pond Dolní Stržený (Dlouhá Lhota, of the Tábor District). Seeds were divided into groups according to 4 treatments: without intervention, scarification, thermal stratification and cold stratification. The field experiment runs from September 2016 to August 2017.The aim was to find out the most appropriate type of management (mowing, disturbance) that would promote seed germination and seedling recruitment. Results from the both experiments: a positive effect of germination after the scarification, better recruitment of seeds in square without intervention, seeds probably needs to be stored for a longer period before sowing. These results cannot be generalized, because small number of repetitions were performed. All the obtained data was discussed and recommendations for the plant management were formulated.
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Changes in timing of germination caused by neighbouring seeds and how it is connected with species traits.
Kos, Pavel ; Weiser, Martin (advisor) ; Hadincová, Věroslava (referee)
The time when the seed germinates is very important. Ability to change the time of germination may be very advantageous. It allows the emerging seed to choose the best time according to abiotic conditions, and also to avoid of competition with neighbouring individuals. The seed reacts not only on adult plants and seedlings, but also on other seeds, with which is able to communicate. For a better understanding to this mechanism I conducted an experimental study with twenty-six species. The species were selected according to their position in long succession seres of mesic/xeric abandoned fields in Český kras. The seeds were left to germinate in pairs in all combinations among them. Here I present the results where I show how the time of emergence changes, depending on presence of neighbouring seed. Also, I show how these changes in germination are related to species specific traits. Out of this, I tried to influence communication between seeds by adding activated carbon. Activated carbon should stop the communication by highly absorbing surface. The time of germination of seeds which germinated alone was not proportional to the time of germination of seeds which germinated with neighbours. This correlation showed up like nonlinear, late-emerging seeds emerging unproportionally later when emerging...
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Seed germination of selected crops after low-temperature treatment
PETRÁŠKOVÁ, Kamila
The influence of low-temperature plasma on the different seeds is currently one of the most used techniques in agriculture. The aim of my bachelor thesis is to monitor and evaluate the effect of low-temperature plasma on the seeds of maize sow (Zea mays L.) and oilseed rape (Brassica napus L. napus), particularly on the germination of the seeds, germination rate and germination energy. These characteristics were measured by germination tests and they are expressed as a percentage. The submitted thesis informs whether the stimulation of the seeds by plasma affects these plants and if so, whether the influence is positive or negative.
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